{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:29:57Z","timestamp":1782188997999,"version":"3.54.5"},"reference-count":40,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,2,28]],"date-time":"2021-02-28T00:00:00Z","timestamp":1614470400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["NRDIO-EDIOP-2.3.2-15-2016-00021"],"award-info":[{"award-number":["NRDIO-EDIOP-2.3.2-15-2016-00021"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["NRDIO-K\/115394\/2015"],"award-info":[{"award-number":["NRDIO-K\/115394\/2015"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["NRDIO-KA-2016-04"],"award-info":[{"award-number":["NRDIO-KA-2016-04"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although non-invasive pre-implantation genetic testing for aneuploidy (NIPGT-A) is potentially appropriate to assess chromosomal ploidy of the embryo, practical application of it in a routine IVF centre have not been started in the absence of a recommendation. Our objective in this study was to provide a comprehensive workflow for a clinically applicable strategy for NIPGT-A based on next-generation sequencing (NGS) technology with the corresponding bioinformatic pipeline. In a retrospective study, we performed NGS on spent blastocyst culture media of Day 3 embryos fertilised with intracytoplasmic sperm injection (ICSI) with quality score on morphology assessment using the blank culture media as background control. Chromosomal abnormalities were identified by an optimised bioinformatics pipeline applying copy number variation (CNV) detecting algorithm. In this study, we demonstrate a comprehensive workflow covering both wet- and dry-lab procedures supporting a clinically applicable strategy for NIPGT-A that can be carried out within 48 h, which is critical for the same-cycle blastocyst transfer. The described integrated approach of non-invasive evaluation of embryonic DNA content of the culture media can potentially supplement existing pre-implantation genetic screening methods.<\/jats:p>","DOI":"10.3390\/ijms22052443","type":"journal-article","created":{"date-parts":[[2021,2,28]],"date-time":"2021-02-28T20:43:32Z","timestamp":1614545012000},"page":"2443","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["NGS-Based Application for Routine Non-Invasive Pre-Implantation Genetic Assessment in IVF"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0388-0942","authenticated-orcid":false,"given":"Katalin","family":"Gombos","sequence":"first","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"},{"name":"Department of Laboratory Medicine, Medical School, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 13., 7624 P\u00e9cs, Hungary"},{"name":"MTA-PTE Human Reproduction Scientific Research Group, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bence","family":"G\u00e1lik","sequence":"additional","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"},{"name":"Department of Clinical Molecular Biology, Medical University of Bialystok, ul. Jana Kilinskiego 1, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina Ildik\u00f3","family":"Kal\u00e1cs","sequence":"additional","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krisztina","family":"G\u00f6d\u00f6ny","sequence":"additional","affiliation":[{"name":"MTA-PTE Human Reproduction Scientific Research Group, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Department of Obstetrics and Gynaecology, Medical School, University of P\u00e9cs, \u00c9desany\u00e1k \u00fatja 17, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c1kos","family":"V\u00e1rnagy","sequence":"additional","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"},{"name":"MTA-PTE Human Reproduction Scientific Research Group, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Department of Obstetrics and Gynaecology, Medical School, University of P\u00e9cs, \u00c9desany\u00e1k \u00fatja 17, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2638-5418","authenticated-orcid":false,"given":"Don\u00e1t","family":"Alp\u00e1r","sequence":"additional","affiliation":[{"name":"MTA-SE Molecular Oncohematology Research Group, 1st Department of Pathology and Experimental Cancer Research, Semmelweis University, \u00dcll\u0151i \u00fat 26., 1085 Budapest, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f3zsef","family":"B\u00f3dis","sequence":"additional","affiliation":[{"name":"MTA-PTE Human Reproduction Scientific Research Group, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"},{"name":"Department of Obstetrics and Gynaecology, Medical School, University of P\u00e9cs, \u00c9desany\u00e1k \u00fatja 17, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Attila","family":"Gyenesei","sequence":"additional","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"},{"name":"Department of Clinical Molecular Biology, Medical University of Bialystok, ul. Jana Kilinskiego 1, 15-089 Bialystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor L.","family":"Kov\u00e1cs","sequence":"additional","affiliation":[{"name":"Szent\u00e1gothai Research Center, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 20., 7624 P\u00e9cs, Hungary"},{"name":"Department of Laboratory Medicine, Medical School, University of P\u00e9cs, Ifj\u00fas\u00e1g \u00fatja 13., 7624 P\u00e9cs, Hungary"},{"name":"MTA-PTE Human Reproduction Scientific Research Group, University of P\u00e9cs, 7624 P\u00e9cs, Hungary"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,2,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology (2011). Istanbul consensus workshop on embryo assessment: Proceedings of an expert meeting. Reprod. Biomed. 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