{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T07:44:33Z","timestamp":1775634273667,"version":"3.50.1"},"reference-count":61,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,13]],"date-time":"2021-03-13T00:00:00Z","timestamp":1615593600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2017\/25\/B\/NZ9\/00090"],"award-info":[{"award-number":["2017\/25\/B\/NZ9\/00090"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>An electromagnetic field (EMF) may affect the functions of uterine tissues. This study hypothesized that EMF changes the estrogenic activity of pig myometrium during the peri-implantation period. Tissue was collected on days 15\u201316 of the gestation and incubated in the presence of EMF (50 and 120 Hz, 2 and 4 h). The cytochrome P450 aromatase type 3 (CYP19A3) and hydroxysteroid 17\u03b2 dehydrogenase type 4 (HSD17B4) mRNA transcript abundance, cytochrome P450arom (aromatase), and 17\u03b2 hydroxysteroid dehydrogenase 17\u03b2HSD) protein abundance and estrone (E1) and estradiol-17\u03b2 (E2) release were examined using Real-Time PCR, Western blot and radioimmunoassay. Selected myometrial slices were treated with progesterone (P4) to determine whether it functions as a protector against EMF. CYP19A3 mRNA transcript abundance in slices treated with EMF was less at 50 Hz (2 h) and greater at 120 Hz (2 and 4 h). HSD17B4 mRNA transcript was greater in slices treated with EMF at 120 Hz (2 h). Progesterone diminished EMF-related effects on CYP19A3 and HSD17B4. When P4 was added, EMF had suppressive (50 and 120 Hz, 2 h) or enhancing (50 Hz, 4 h) effects on aromatase abundance. The E1 release was lower after 4 h of EMF treatment at 50 Hz and P4 did not protect myometrial E1 release. In conclusion, EMF alters the synthesis and release of E1 and did not affect E2 release in the myometrium during the peri-implantation period.<\/jats:p>","DOI":"10.3390\/ijms22062920","type":"journal-article","created":{"date-parts":[[2021,3,14]],"date-time":"2021-03-14T22:13:10Z","timestamp":1615759990000},"page":"2920","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Electromagnetic Field (EMF) Radiation Alters Estrogen Release from the Pig Myometrium during the Peri-Implantation Period"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1328-0190","authenticated-orcid":false,"given":"Ewa Monika","family":"Drzewiecka","sequence":"first","affiliation":[{"name":"Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wiktoria","family":"Kozlowska","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0661-130X","authenticated-orcid":false,"given":"Agata","family":"Zmijewska","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pawel Jozef","family":"Wydorski","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anita","family":"Franczak","sequence":"additional","affiliation":[{"name":"Department of Animal Anatomy and Physiology, Faculty of Biology and Biotechnology, University of Warmia and Mazury in Olsztyn, Oczapowskiego 1A, 10-719 Olsztyn, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"925","DOI":"10.1095\/biolreprod27.4.925","article-title":"Establishment of pregnancy in the pig: I. Interrelationships between preimplantation development of the pig blastocyst and uterine endometrial secretions","volume":"27","author":"Geisert","year":"1982","journal-title":"Biol. Reprod."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1111\/j.1439-0531.2011.01843.x","article-title":"Mechanisms for the establishment of pregnancy in the Pig","volume":"46","author":"Ziecik","year":"2011","journal-title":"Reprod. Domest. Anim."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1530\/REP-17-0662","article-title":"Regulation of the porcine corpus luteum during pregnancy","volume":"156","author":"Ziecik","year":"2018","journal-title":"Reproduction"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.diff.2013.11.005","article-title":"Pig blastocyst-uterine interactions","volume":"87","author":"Bazer","year":"2014","journal-title":"Differentiation"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"842","DOI":"10.1002\/mrd.22835","article-title":"Embryo-maternal dialogue during pregnancy establishment and implantation in the pig","volume":"84","author":"Waclawik","year":"2017","journal-title":"Mol. Reprod. Dev."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S1642-431X(12)60013-8","article-title":"Endometrial and myometrial secretion of androgens and estrone during early pregnancy and luteolysis in pigs","volume":"8","author":"Franczak","year":"2008","journal-title":"Reprod. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.theriogenology.2007.09.023","article-title":"Secretion of estradiol-17\u03b2 by porcine endometrium and myometrium during early pregnancy and luteolysis","volume":"69","author":"Franczak","year":"2008","journal-title":"Theriogenology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"232","DOI":"10.1016\/j.theriogenology.2009.09.004","article-title":"Androgens and estradiol-17\u03b2 production by porcine uterine cells: In vitro study","volume":"73","author":"Franczak","year":"2010","journal-title":"Theriogenology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"253","DOI":"10.3409\/fb61_3-4.253","article-title":"Novel aspects of cytokine action in porcine uterus\u2014Endometrial and myometrial production of estrone (E1) in the presence of interleukin 1\u03b2 (IL1\u03b2), interleukin 6 (IL6) and tumor necrosis factor (TNF\u03b1)\u2014In vitro study","volume":"61","author":"Franczak","year":"2013","journal-title":"Folia Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1016\/j.theriogenology.2013.09.024","article-title":"The effect of interleukin-1\u03b2, interleukin-6, and tumor necrosis factor-\u03b1 on estradiol-17\u03b2 release in the myometrium: The in vitro study on the pig model","volume":"81","author":"Franczak","year":"2014","journal-title":"Theriogenology"},{"key":"ref_11","first-page":"443","article-title":"Effect of adiponectin on the steroidogenic acute regulatory protein, P450 side chain cleavage enzyme and 3\u03b2-hydroxysteroid dehydrogenase gene expression, progesterone and androstenedione production by the porcine uterus during early pregnancy","volume":"67","author":"Smolinska","year":"2016","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1016\/j.theriogenology.2016.11.028","article-title":"The effect of orexin A on CYP17A1 and CYP19A3 expression and on oestradiol, oestrone and testosterone secretion in the porcine uterus during early pregnancy and the oestrous cycle","volume":"90","author":"Kiezun","year":"2017","journal-title":"Theriogenology"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"102","DOI":"10.1016\/j.theriogenology.2018.10.026","article-title":"Expression of vitamin D receptor in the porcine uterus and effect of 1,25(OH)2D3 on progesterone and estradiol-17\u03b2 secretion by uterine tissues in vitro","volume":"125","author":"Grzesiak","year":"2019","journal-title":"Theriogenology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"851","DOI":"10.1093\/jas\/sky458","article-title":"The effect of orexin B on steroidogenic acute regulatory protein, P450 side-chain cleavage enzyme, and 3\u03b2-hydroxysteroid dehydrogenase gene expression, and progesterone and androstenedione secretion by the porcine uterus during early pregnancy and the est","volume":"97","author":"Kisielewska","year":"2019","journal-title":"J. Anim. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.theriogenology.2019.10.012","article-title":"The effect of orexin a on the StAR, CYP11A1 and HSD3B1 gene expression, as well as progesterone and androstenedione secretion in the porcine uterus during early pregnancy and the oestrous cycle","volume":"143","author":"Rytelewska","year":"2020","journal-title":"Theriogenology"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Waszkiewicz, E.M., Kozlowska, W., Zmijewska, A., and Franczak, A. (2020). Expression of insulin-like growth factor 1 (IGF-1) and epidermal growth factor (EGF) receptors and the effect of IGF-1 and EGF on androgen and estrogen release in the myometrium of pigs\u2014In vitro study. Animals, 10.","DOI":"10.3390\/ani10050915"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1200","DOI":"10.1071\/RD20148","article-title":"Effects of LH and FSH on androgen and oestrogen release in the myometrium of pigs during the oestrous cycle and early pregnancy","volume":"32","author":"Waszkiewicz","year":"2020","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1016\/j.theriogenology.2017.12.041","article-title":"Extremely low-frequency electromagnetic field (EMF) generates alterations in the synthesis and secretion of oestradiol-17\u03b2 (E2) in uterine tissues: An in vitro study","volume":"110","author":"Koziorowska","year":"2018","journal-title":"Theriogenology"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"106465","DOI":"10.1016\/j.anireprosci.2020.106465","article-title":"Consequences of electromagnetic field (EMF) radiation during early pregnancy\u2014Androgen synthesis and release from the myometrium of pigs in vitro","volume":"218","author":"Franczak","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"106694","DOI":"10.1016\/j.anireprosci.2021.106694","article-title":"Effects of electromagnetic field (EMF) radiation on androgen synthesis and release from the pig endometrium during the fetal peri-implantation period","volume":"226","author":"Kozlowska","year":"2021","journal-title":"Anim. Reprod. Sci."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Gaj\u0161ek, P., Ravazzani, P., Grellier, J., Samaras, T., Bakos, J., and Thur\u00f3czy, G. (2016). Review of studies concerning electromagnetic field (EMF) exposure assessment in Europe: Low frequency fields (50 Hz\u2013100 kHz). Int. J. Environ. Res. Public Health, 13.","DOI":"10.3390\/ijerph13090875"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"106258","DOI":"10.1016\/j.anireprosci.2019.106258","article-title":"In vitro study of effects of ELF-EMF on testicular tissues of roe deer (Capreolus capreolus)\u2014FTIR and FT-Raman spectroscopic investigation","volume":"213","author":"Koziorowska","year":"2020","journal-title":"Anim. Reprod. Sci."},{"key":"ref_23","first-page":"468","article-title":"Effects of exposure to extremely low frequency electromagnetic fields on reproduction of female mice and development of offsprings","volume":"24","author":"Cao","year":"2006","journal-title":"Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi"},{"key":"ref_24","first-page":"60","article-title":"An overview on the effects of power frequency electromagnetic field exposure on the female reproduction system, pregnancy outcome and fetal development","volume":"3","author":"Mansuori","year":"2020","journal-title":"J. Med. Chem. Sci."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1088\/2399-6528\/ab66d2","article-title":"An extremely low-frequency magnetic field can affect CREB protein conformation which may have a role in neuronal activities including memory","volume":"4","author":"Darwish","year":"2020","journal-title":"J. Phys. Commun."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"24","DOI":"10.15171\/ijoem.2020.1572","article-title":"Effects of Various Densities of 50 Hz Electromagnetic Field on Serum IL-9, IL-10, and TNF-\u03b1 Levels","volume":"11","author":"Mahaki","year":"2020","journal-title":"Int. J. Occup. Environ. Med."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1210\/er.2003-0030","article-title":"Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones","volume":"25","author":"Payne","year":"2004","journal-title":"Endocr. Rev."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1016\/0093-691X(91)90403-Z","article-title":"The extent and timing of prenatal loss in gilts","volume":"36","author":"Lambert","year":"1991","journal-title":"Theriogenology"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"61","DOI":"10.1016\/0090-6980(94)90075-2","article-title":"Luteal maintenance during early pregnancy in the pig: Role for prostaglandin E2","volume":"47","author":"Christenson","year":"1994","journal-title":"Prostaglandins"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3823","DOI":"10.1210\/en.2008-1499","article-title":"Estradiol-17\u03b2, prostaglandin E2 (PGE2), and the PGE2 receptor are involved in PGE2 positive feedback loop in the porcine endometrium","volume":"150","author":"Waclawik","year":"2009","journal-title":"Endocrinology"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0739-7240(02)00162-5","article-title":"Old, new and the newest concepts of inhibition of luteolysis during early pregnancy in pig","volume":"23","author":"Ziecik","year":"2002","journal-title":"Domest. Anim. Endocrinol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1095\/biolreprod.103.024133","article-title":"Progesterone and placental hormone actions on the uterus: Insights from domestic animals","volume":"71","author":"Spencer","year":"2004","journal-title":"Biol. Reprod."},{"key":"ref_33","first-page":"D4395","article-title":"Effectiveness of progesterone in pregnancy complications","volume":"163","author":"Hoorn","year":"2019","journal-title":"Ned. Tijdschr. Geneeskd."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"108","DOI":"10.1016\/j.bpobgyn.2020.06.001","article-title":"Progesterone, Reproduction, and Psychiatric Illness","volume":"69","author":"Standeven","year":"2020","journal-title":"Best Pract. Res. Clin. Obstet. Gynaecol."},{"key":"ref_35","unstructured":"Berg, J.M., and Tymoczko, J.L. (2002). 28.2 Eukaryotic Transcription and Translation Are Separated in Space and Time. Biochemistry, W H Freeman."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1016\/0039-128X(86)90002-4","article-title":"Cytochromes P-450 and the regulation of steroid synthesis","volume":"48","author":"Hall","year":"1986","journal-title":"Steroids"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1210\/er.2010-0013","article-title":"The molecular biology, biochemistry, and physiology of human steroidogenesis and its disorders","volume":"32","author":"Miller","year":"2011","journal-title":"Endocr. Rev."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1093\/humupd\/dmu008","article-title":"Androgens in pregnancy: Roles in parturition","volume":"20","author":"Makieva","year":"2014","journal-title":"Hum. Reprod. Update"},{"key":"ref_39","first-page":"342","article-title":"Aromatase cytochrome p450, the enzyme responsible for estrogen biosynthesis","volume":"15","author":"Simpson","year":"1994","journal-title":"Endocr. Rev."},{"key":"ref_40","first-page":"733","article-title":"Interleukin 1\u03b2-induced synthesis and secretion of prostaglandin E2 in the porcine uterus during various periods of pregnancy and the estrous cycle","volume":"61","author":"Franczak","year":"2010","journal-title":"J. Physiol. Pharmacol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"487","DOI":"10.1016\/j.cellsig.2015.01.004","article-title":"Hedgehog signaling stimulates the conversion of cholesterol to steroids","volume":"27","author":"Tang","year":"2015","journal-title":"Cell. Signal."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1210\/me.2007-0443","article-title":"Progesterone receptor inhibits aromatase and inflammatory response pathways in breast cancer cells via ligand-dependent and ligand-independent mechanisms","volume":"22","author":"Hardy","year":"2008","journal-title":"Mol. Endocrinol."},{"key":"ref_43","doi-asserted-by":"crossref","unstructured":"Gry, M., Rimini, R., Str\u00f6mberg, S., Asplund, A., Pont\u00e9n, F., Uhl\u00e9n, M., and Nilsson, P. (2009). Correlations between RNA and protein expression profiles in 23 human cell lines. BMC Genomics, 10.","DOI":"10.1186\/1471-2164-10-365"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/0090-6980(77)90185-X","article-title":"Theory of maternal recognition of pregnancy in swine based on estrogen controlled endocrine versus exocrine secretion of prostaglandin F2\u03b1 by the uterine endometrium","volume":"14","author":"Bazer","year":"1977","journal-title":"Prostaglandins"},{"key":"ref_45","first-page":"3342","article-title":"Biochemistry of aromatase: Significance to female reproductive physiology","volume":"42","author":"Ryan","year":"1982","journal-title":"Cancer Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4149\/endo_2014_04_201","article-title":"Impact of endocrine disruptors on ovarian steroidogenesis","volume":"48","author":"Mlynarcikova","year":"2014","journal-title":"Endocr. Regul."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.ygcen.2016.03.003","article-title":"Immune-endocrine crosstalk during pregnancy","volume":"242","author":"Nair","year":"2017","journal-title":"Gen. Comp. Endocrinol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1530\/jrf.0.0870125","article-title":"Direct luteotrophic effect of oestradiol-17\u03b2 on pig corpora lutea","volume":"87","author":"Conley","year":"1989","journal-title":"J. Reprod. Fertil."},{"key":"ref_49","first-page":"131","article-title":"Maternal recognition of pregnancy signal or endocrine disruptor: The two faces of oestrogen during establishment of pregnancy in the pig","volume":"62","author":"Geisert","year":"2006","journal-title":"Soc. Reprod. Fertil. Suppl."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1071\/RD15430","article-title":"Expression of 17\u03b2-hydroxysteroid dehydrogenase and the effects of LH, FSH and prolactin on oestrone and 17\u03b2-oestradiol secretion in the endometrium of pigs during early pregnancy and the oestrous cycle","volume":"29","author":"Wojciechowicz","year":"2017","journal-title":"Reprod. Fertil. Dev."},{"key":"ref_51","first-page":"1512","article-title":"Global signatures of protein and mRNA expression levels","volume":"5","author":"Penalva","year":"2009","journal-title":"Mol. Biosyst."},{"key":"ref_52","first-page":"1953","article-title":"Gross ovarian changes during estrous cycle of swine","volume":"29","author":"Akins","year":"1968","journal-title":"Am. J. Vet. Res."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1016\/0093-691X(94)90379-W","article-title":"Follicular development during early pregnancy and the estrous cycle of the sow","volume":"42","author":"Ryan","year":"1994","journal-title":"Theriogenology"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1007\/BF00318976","article-title":"Electron microscopy of the initial stages of placentation in the pig","volume":"172","author":"Dantzer","year":"1985","journal-title":"Anat. Embryol."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1439-0531.2009.01482.x","article-title":"From zygote to implantation: Morphological and molecular dynamics during embryo development in the pig","volume":"44","author":"Oestrup","year":"2009","journal-title":"Reprod. Domest. Anim."},{"key":"ref_56","first-page":"1067","article-title":"The impact of electromagnetic field at a frequency of 50 Hz and a magnetic induction of 2.5 mT on viability of pineal cells in vitro","volume":"30","author":"Koziorowska","year":"2016","journal-title":"J. Biol. Regul. Homeost. Agents"},{"key":"ref_57","unstructured":"Grandolfo, M. (1994). Protection of workers from power frequency electric and magnetic fields: A practical guide. Occupational Safety and Health Series, International Labour Office."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1373\/clinchem.2008.112797","article-title":"The MIQE guidelines: Minimum information for publication of quantitative real-time PCR experiments","volume":"55","author":"Bustin","year":"2009","journal-title":"Clin. Chem."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_60","unstructured":"Przala, J. (2009). Radioimmunoassay of Steroid Hormones in Biological Fluids. Demonstrations and Methods, University of Warmia and Mazury Press."},{"key":"ref_61","first-page":"187","article-title":"Primary antisera against selected steroids or proteins and secondary antisera against gamma-globulins\u2014An available tool for studies of reproductive processes","volume":"2","author":"Ziecik","year":"2002","journal-title":"Reprod. Biol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/6\/2920\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:35:14Z","timestamp":1760160914000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/6\/2920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,13]]},"references-count":61,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,3]]}},"alternative-id":["ijms22062920"],"URL":"https:\/\/doi.org\/10.3390\/ijms22062920","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,13]]}}}