{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T12:18:48Z","timestamp":1775737128553,"version":"3.50.1"},"reference-count":31,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T00:00:00Z","timestamp":1616025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP19K07041"],"award-info":[{"award-number":["JP19K07041"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Plasmabio consortium","award":["01221910"],"award-info":[{"award-number":["01221910"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cold atmospheric plasma (CAP) has attracted much attention in the fields of biotechnology and medicine owing to its potential utility in clinical applications. Recently accumulating evidence has demonstrated that CAP influences protein structures. However, there remain open questions regarding the molecular mechanisms behind the CAP-induced structural perturbations of biomacromolecules. Here, we investigated the potential effects of CAP irradiation of amyloid \u03b2 (A\u03b2), an amyloidogenic protein associated with Alzheimer\u2019s disease. Using nuclear magnetic resonance spectroscopy, we observed gradual spectral changes in A\u03b2 after a 10 s CAP pretreatment, which also suppressed its fibril formation, as revealed by thioflavin T assay. As per mass spectrometric analyses, these effects were attributed to selective oxidation of the methionine residue (Met) at position 35. Interestingly, this modification occurred when A\u03b2 was dissolved into a pre-irradiated buffer, indicating that some reactive species oxidize the Met residue. Our results strongly suggest that the H2O2 generated in the solution by CAP irradiation is responsible for Met oxidation, which inhibits A\u03b2 amyloid formation. The findings of the present study provide fundamental insights into plasma biology, giving clues for developing novel applications of CAP.<\/jats:p>","DOI":"10.3390\/ijms22063116","type":"journal-article","created":{"date-parts":[[2021,3,18]],"date-time":"2021-03-18T11:41:06Z","timestamp":1616067666000},"page":"3116","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Cold Atmospheric Plasma Modification of Amyloid \u03b2"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8144-740X","authenticated-orcid":false,"given":"Maho","family":"Yagi-Utsumi","sequence":"first","affiliation":[{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"Institute for Molecular Science (IMS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7442-4912","authenticated-orcid":false,"given":"Tomohiro","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"Department of Plasmabio Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Minato-ku, Tokyo 105-0001, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2401-4808","authenticated-orcid":false,"given":"Yoko","family":"Otsubo","sequence":"additional","affiliation":[{"name":"Department of Plasmabio Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Minato-ku, Tokyo 105-0001, Japan"},{"name":"National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, Japan"},{"name":"National Institute for Fusion Science, National Institutes of Natural Sciences, Toki, Gifu 509-5292, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1805-1434","authenticated-orcid":false,"given":"Akira","family":"Yamashita","sequence":"additional","affiliation":[{"name":"Department of Plasmabio Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Minato-ku, Tokyo 105-0001, Japan"},{"name":"National Institute for Basic Biology, National Institutes of Natural Sciences, Okazaki, Aichi 444-8585, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0602-0665","authenticated-orcid":false,"given":"Shinji","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Department of Plasmabio Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Minato-ku, Tokyo 105-0001, Japan"},{"name":"National Institute for Fusion Science, National Institutes of Natural Sciences, Toki, Gifu 509-5292, Japan"},{"name":"Center for Low-temperature Plasma Sciences, Nagoya University, Nagoya 464-8601, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2587-5458","authenticated-orcid":false,"given":"Motohiro","family":"Nishida","sequence":"additional","affiliation":[{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"National Institute for Physiological Sciences, National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"Department of Plasmabio Science, Center for Novel Science Initiatives, National Institutes of Natural Sciences, Minato-ku, Tokyo 105-0001, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7187-9612","authenticated-orcid":false,"given":"Koichi","family":"Kato","sequence":"additional","affiliation":[{"name":"Exploratory Research Center on Life and Living Systems (ExCELLS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"},{"name":"Institute for Molecular Science (IMS), National Institutes of Natural Sciences, Okazaki, Aichi 444-8787, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2019\/3873928","article-title":"Plasma Medicine: Applications of Cold Atmospheric Pressure Plasma in Dermatology","volume":"2019","author":"Bernhardt","year":"2019","journal-title":"Oxidative Med. 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