{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,4]],"date-time":"2026-07-04T00:05:02Z","timestamp":1783123502611,"version":"3.54.6"},"reference-count":63,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T00:00:00Z","timestamp":1616630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union funds, POWER 2014-2020","award":["\u2116 POWR.03.02.00-00-I051\/16  and \u2116. 05\/IMSD\/G\/2019"],"award-info":[{"award-number":["\u2116 POWR.03.02.00-00-I051\/16  and \u2116. 05\/IMSD\/G\/2019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Jasione montana L. (Campanulaceae) is used in traditional Belarusian herbal medicine for sleep disorders in children, but the chemical composition and biological activity have not been investigated. In this study, the activities of J. montana extracts, their fractions and main compounds were evaluated in amelanotic melanoma C32 (CRL-1585) cells and normal fibroblasts (PCS-201-012). The extracts and fractions were analyzed using liquid chromatography\u2013photodiode array detection\u2013electrospray ionization\u2013mass spectrometry (LC\u2013PDA\u2013ESI\u2013MS\/TOF) to characterize 25 compounds. Further, three major and known constituents, luteolin (22) and its derivatives such as 7-O-glucoside (12) and 7-O-sambubioside (9) were isolated and identified. The cytotoxic activities against fibroblasts and the amelanotic melanoma cell line were determined using the fixable viability stain (FVS) assay. The influence of diethyl ether (Et2O) fraction (JM4) and 22 on apoptosis induction was investigated using an annexin V binding assay. The obtained results showed significant cytotoxicity of JM4 and 22 with IC50 values of 119.7 \u00b1 3.2 and 95.1 \u00b1 7.2 \u03bcg\/mL, respectively. The proapoptotic potential after 22 treatment in the C32 human amelanotic melanoma cell line was comparable to that of vinblastine sulfate (VLB), detecting 29.2 \u00b1 3.0% apoptotic cells. Moreover, 22 displayed less necrotic potential against melanoma cells than VLB. In addition, the influences of JM4 and 22 on the dysfunction of the mitochondrial membrane potential (MMP), cell cycle and activity of caspases 3, 8, 9, and 10 were established. The effects of JM4 on MMP change (74.5 \u00b1 3.0% of the cells showed a reduced MMP) corresponded to the results obtained from the annexin V binding assay and activation of caspase-9. JM4 and 22 displayed a significant impact on caspase-9 (40.9 \u00b1 2.4% of the cells contained active caspase-9 after JM4 treatment and 16.6 \u00b1 0.8% after incubation with 22) and the intrinsic (mitochondrial) apoptotic pathway. Moreover, studies have shown that JM4 and 22 affect the activation of external apoptosis pathways by inducing the caspase-8 and caspase-10 cascades. Thus, activation of caspase-3 and DNA damage via external and internal apoptotic pathways were observed after treatment with JM4 and 22. The obtained results suggest that J. montana extracts could be developed as new topical preparations with potential anticancer properties due to their promising cytotoxic and proapoptotic potential.<\/jats:p>","DOI":"10.3390\/ijms22073345","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T14:51:12Z","timestamp":1616683872000},"page":"3345","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["In Vitro Anticancer Potential of Jasione montana and Its Main Components against Human Amelanotic Melanoma Cells"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3072-9869","authenticated-orcid":false,"given":"Aleksandra Maria","family":"Juszczak","sequence":"first","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bia\u0142ystok, ul. Mickiewicza 2a, 15-230 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7148-6111","authenticated-orcid":false,"given":"Robert","family":"Czarnomysy","sequence":"additional","affiliation":[{"name":"Department of Synthesis and Technology of Drugs, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bia\u0142ystok, ul. Kili\u0144skiego 1, 15-089 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7133-1817","authenticated-orcid":false,"given":"Jakub W\u0142adys\u0142aw","family":"Strawa","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bia\u0142ystok, ul. Mickiewicza 2a, 15-230 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8787-6667","authenticated-orcid":false,"given":"Marijana","family":"Zovko Kon\u010di\u0107","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Maruli\u0107ev trg 20\/II, 10000 Zagreb, Croatia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Bielawski","sequence":"additional","affiliation":[{"name":"Department of Synthesis and Technology of Drugs, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bia\u0142ystok, ul. Kili\u0144skiego 1, 15-089 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4063-1048","authenticated-orcid":false,"given":"Micha\u0142","family":"Tomczyk","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Faculty of Pharmacy with the Division of Laboratory Medicine, Medical University of Bia\u0142ystok, ul. Mickiewicza 2a, 15-230 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,25]]},"reference":[{"key":"ref_1","unstructured":"World Health Organization (2020, January 28). Radiation: Ultraviolet (UV) Radiation and Skin Cancer. 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