{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:37:59Z","timestamp":1791549479937,"version":"4.3.4"},"reference-count":47,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T00:00:00Z","timestamp":1616630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Cervical cancer is the third most common in Brazilian women. The chemotherapy used for the treatment of this disease can cause many side effects; then, to overcome this problem, new treatment options are necessary. Natural compounds represent one of the most promising sources for the development of new drugs. In this study, 13 different species of 6 families from the Brazilian Cerrado vegetation biome were screened against human cervical cancer cell lines (CCC). Some of these species were also evaluated in one normal keratinocyte cell line (HaCaT). The effect of crude extracts on cell viability was evaluated by a colorimetric method (MTS assay). Extracts from Annona crassiflora, Miconia albicans, Miconia chamissois, Stryphnodendron adstringens, Tapirira guianensis, Xylopia aromatica, and Achyrocline alata showed half-maximal inhibitory concentration (IC50) values &lt; 30 \u03bcg\/mL for at least one CCC. A. crassiflora and S. adstringens extracts were selective for CCC. Mass spectrometry (Electrospray Ionization Fourier Transform Ion Cyclotron Resonance Mass Spectrometer (ESI FT-ICR MS)) of A. crassiflora identified fatty acids and flavonols as secondary compounds. One of the A. crassiflora fractions, 7C24 (from chloroform partition), increased H2AX phosphorylation (suggesting DNA damage), PARP cleavage, and cell cycle arrest in CCC. Kaempferol-3-O-rhamnoside and oleic acid were bioactive molecules identified in 7C24 fraction. These findings emphasize the importance of investigating bioactive molecules from natural sources for developing new anti-cancer drugs.<\/jats:p>","DOI":"10.3390\/ijms22073383","type":"journal-article","created":{"date-parts":[[2021,3,25]],"date-time":"2021-03-25T14:51:12Z","timestamp":1616683872000},"page":"3383","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Bioprospecting of Natural Compounds from Brazilian Cerrado Biome Plants in Human Cervical Cancer Cell Lines"],"prefix":"10.3390","volume":"22","author":[{"given":"Marcela N.","family":"Rosa","sequence":"first","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa R. V.","family":"e Silva","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5828-8957","authenticated-orcid":false,"given":"Giovanna B.","family":"Longato","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"},{"name":"Research Laboratory in Molecular Pharmacology and Bioactive Compounds, S\u00e3o Francisco University, Bragan\u00e7a Paulista 12916-900, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4731-2082","authenticated-orcid":false,"given":"Adriane F.","family":"Evangelista","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izabela N. F.","family":"Gomes","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8672-3768","authenticated-orcid":false,"given":"Ana Laura V.","family":"Alves","sequence":"additional","affiliation":[{"name":"Molecular Oncology Research Center, Barretos Cancer Hospital, Barretos 14784-400, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8561-126X","authenticated-orcid":false,"given":"Bruno G.","family":"de Oliveira","sequence":"additional","affiliation":[{"name":"Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Esp\u00edrito Santo, Vit\u00f3ria 29075-910, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fernanda E.","family":"Pinto","sequence":"additional","affiliation":[{"name":"Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Esp\u00edrito Santo, Vit\u00f3ria 29075-910, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanderson","family":"Rom\u00e3o","sequence":"additional","affiliation":[{"name":"Petroleomic and Forensic Laboratory, Chemistry Department, Federal University of Esp\u00edrito Santo, Vit\u00f3ria 29075-910, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allisson R.","family":"de Rezende","sequence":"additional","affiliation":[{"name":"Higher Institute of Education and Research of Ituiutaba, University of the State of Minas Gerais (UEMG), Ituiutaba 38302-192, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arali A. C.","family":"Ara\u00fajo","sequence":"additional","affiliation":[{"name":"Higher Institute of Education and Research of Ituiutaba, University of the State of Minas Gerais (UEMG), Ituiutaba 38302-192, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lohanna S. F. M.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology, Federal University of S\u00e3o Jo\u00e3o del Rei\u2014CCO\/UFSJ, Divin\u00f3polis 35501-296, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandra A.","family":"de M. Souza","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology, Federal University of S\u00e3o Jo\u00e3o del Rei\u2014CCO\/UFSJ, Divin\u00f3polis 35501-296, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology, Federal University of S\u00e3o Jo\u00e3o del Rei\u2014CCO\/UFSJ, Divin\u00f3polis 35501-296, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosy Iara M.","family":"de A. Ribeiro","sequence":"additional","affiliation":[{"name":"Laboratory of Experimental Pathology, Federal University of S\u00e3o Jo\u00e3o del Rei\u2014CCO\/UFSJ, Divin\u00f3polis 35501-296, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5919-5242","authenticated-orcid":false,"given":"Viviane A. 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