{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:13:57Z","timestamp":1790856837952,"version":"4.1.0"},"reference-count":37,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,26]],"date-time":"2021-03-26T00:00:00Z","timestamp":1616716800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Janet and Edward Gildea Charitable Foundation","award":["none"],"award-info":[{"award-number":["none"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We studied cell proliferation in the postnatal mouse brain between the ages of 2 and 30 months and identified four compartments with different densities of proliferating cells. The first identified compartment corresponds to the postnatal pallial neurogenic (PPN) zone in the telencephalon; the second to the subpallial postnatal neurogenic (SPPN) zone in the telencephalon; the third to the white matter bundles in the telencephalon; and the fourth to all brain parts outside of the other three compartments. We estimated that about 3.4 million new cells, including 0.8 million in the subgranular zone (SGZ) in the hippocampus, are produced in the PPN zone. About 21 million new cells, including 10 million in the subependymal zone (SEZ) in the lateral walls of the lateral ventricle and 2.7 million in the rostral migratory stream (RMS), are produced in the SPPN zone. The third and fourth compartments together produced about 31 million new cells. The analysis of cell proliferation in neurogenic zones shows that postnatal neurogenesis is the direct continuation of developmental neurogenesis in the telencephalon and that adult neurogenesis has characteristics of the late developmental process. As a developmental process, adult neurogenesis supports only compensatory regeneration, which is very inefficient.<\/jats:p>","DOI":"10.3390\/ijms22073449","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T22:09:01Z","timestamp":1616969341000},"page":"3449","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Proliferative Capacity of Adult Mouse Brain"],"prefix":"10.3390","volume":"22","author":[{"given":"Mikhail","family":"Semenov","sequence":"first","affiliation":[{"name":"The Department of Pathology and Laboratory Medicine, Boston University School of Medicine, Boston University, Boston, MA 02215, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1007\/s10072-006-0682-z","article-title":"The end of the central dogma of neurobiology: Stem cells and neurogenesis in adult CNS","volume":"27","author":"Bonavita","year":"2006","journal-title":"Neurol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1002\/bies.20703","article-title":"Neurogenesis in adult CNS: From denial to opportunities and challenges for therapy","volume":"30","year":"2008","journal-title":"Bioessays"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1002\/cne.901240303","article-title":"Autoradiographic and histological evidence of postnatal hippocampal neurogenesis in rats","volume":"124","author":"Altman","year":"1965","journal-title":"J. Comp. Neurol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1016\/j.stem.2015.09.003","article-title":"Adult Mammalian Neural Stem Cells and Neurogenesis: Five Decades Later","volume":"17","author":"Bond","year":"2015","journal-title":"Cell Stem Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"44","DOI":"10.3389\/fnins.2016.00044","article-title":"Adult Neurogenesis 50 Years Later: Limits and Opportunities in Mammals","volume":"10","author":"Bbonfanti","year":"2016","journal-title":"Front. Neurosci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1002\/cne.902400204","article-title":"Ultrastructural characterization of synaptic terminals formed on newly generated neurons in a song control nucleus of the adult canary forebrain","volume":"240","author":"Burd","year":"1985","journal-title":"J. Comp. Neurol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1046","DOI":"10.1126\/science.6474166","article-title":"Neurons generated in the adult brain are recruited into functional circuits","volume":"225","author":"Paton","year":"1984","journal-title":"Science"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1707","DOI":"10.1126\/science.1553558","article-title":"Generation of neurons and astrocytes from isolated cells of the adult mammalian central nervous system","volume":"255","author":"Reynolds","year":"1992","journal-title":"Science"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"8591","DOI":"10.1073\/pnas.89.18.8591","article-title":"De novo generation of neuronal cells from the adult mouse brain","volume":"89","author":"Richards","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"703","DOI":"10.1016\/S0092-8674(00)80783-7","article-title":"Subventricular Zone Astrocytes Are Neural Stem Cells in the Adult Mammalian Brain","volume":"97","author":"Doetsch","year":"1999","journal-title":"Cell"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"7153","DOI":"10.1523\/JNEUROSCI.21-18-07153.2001","article-title":"Astrocytes Give Rise to New Neurons in the Adult Mammalian Hippocampus","volume":"21","author":"Seri","year":"2001","journal-title":"J. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Bordiuk, O.L., Smith, K., Morin, P.J., and Sem\u00ebnov, M.V. (2014). Cell Proliferation and Neurogenesis in Adult Mouse Brain. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0111453"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1001\/archneur.64.5.639","article-title":"Contribution of Intermediate Progenitor Cells to Cortical Histogenesis","volume":"64","author":"Noctor","year":"2007","journal-title":"Arch. Neurol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"20994","DOI":"10.1073\/pnas.0807059105","article-title":"Neurogenesis and widespread forebrain migration of distinct GABAergic neurons from the postnatal subventricular zone","volume":"105","author":"Inta","year":"2008","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1038\/nn1479","article-title":"Neuronal fate determinants of adult olfactory bulb neurogenesis","volume":"8","author":"Hack","year":"2005","journal-title":"Nat. Neurosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"173","DOI":"10.1016\/0896-6273(93)90281-U","article-title":"Restricted proliferation and migration of postnatally generated neurons derived from the forebrain subventricular zone","volume":"11","author":"Luskin","year":"1993","journal-title":"Neuron"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"381","DOI":"10.1126\/science.1144914","article-title":"Mosaic Organization of Neural Stem Cells in the Adult Brain","volume":"317","author":"Merkle","year":"2007","journal-title":"Science"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3201","DOI":"10.1172\/JCI90602","article-title":"Microglia in steady state","volume":"127","author":"Kierdorf","year":"2017","journal-title":"J. Clin. Investig."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Verkhratsky, A., Ho, M.S., Zorec, R., and Parpura, V. (2019). Physiology of Oligodendroglia. Neuroglia in Neurodegenerative Diseases, Advances in Experimental Medicine and Biology, Springer.","DOI":"10.1007\/978-981-13-9913-8"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"8173","DOI":"10.1523\/JNEUROSCI.0928-12.2012","article-title":"Properties and Fate of Oligodendrocyte Progenitor Cells in the Corpus Callosum, Motor Cortex, and Piriform Cortex of the Mouse","volume":"32","author":"Clarke","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1016\/j.brainres.2015.08.013","article-title":"Lineage, fate, and fate potential of NG2-glia","volume":"1638","author":"Nishiyama","year":"2016","journal-title":"Brain Res."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.stem.2011.03.010","article-title":"Division-Coupled Astrocytic Differentiation and Age-Related Depletion of Neural Stem Cells in the Adult Hippocampus","volume":"8","author":"Encinas","year":"2011","journal-title":"Cell Stem Cell"},{"key":"ref_23","first-page":"84","article-title":"A Cell Atlas for the Mouse Brain","volume":"12","author":"Gewaltig","year":"2018","journal-title":"Front. Aging Neurosci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"i103","DOI":"10.1093\/cercor\/bhk027","article-title":"Composition and Organization of the SCZ: A Large Germinal Layer Containing Neural Stem Cells in the Adult Mammalian Brain","volume":"16","author":"Seri","year":"2006","journal-title":"Cereb. Cortex"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"599","DOI":"10.1111\/j.1460-9568.2010.07557.x","article-title":"Evidence for the spontaneous production but massive programmed cell death of new neurons in the subcallosal zone of the postnatal mouse brain","volume":"33","author":"Kim","year":"2011","journal-title":"Eur. J. Neurosci."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Williams, R.W. (2000). Mapping Genes that Modulate Mouse Brain Development: A Quantitative Genetic Approach. Mouse Brain Development, Springer.","DOI":"10.1007\/978-3-540-48002-0_2"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"225","DOI":"10.1038\/s41593-020-00759-4","article-title":"Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging","volume":"24","author":"Bottes","year":"2021","journal-title":"Nat. Neurosci."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Harris, L., Rigo, P., Stiehl, T., Gaber, Z.B., Austin, S.H., Masdeu, M.D.M., Edwards, A., Urb\u00e1n, N., Marciniak-Czochra, A., and Guillemot, F. (2021). Coordinated changes in cellular behavior ensure the lifelong maintenance of the hippocampal stem cell population. Cell Stem Cell.","DOI":"10.1016\/j.stem.2021.01.003"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"159","DOI":"10.3389\/fnins.2019.00159","article-title":"Adult Hippocampal Neurogenesis Is a Developmental Process Involved in Cognitive Development","volume":"13","year":"2019","journal-title":"Front. Neurosci."},{"key":"ref_30","unstructured":"Hebb, D. (1949). The Organization of Behavior, John Wiley & Sons, Inc."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.1038\/nn.2185","article-title":"Roles of continuous neurogenesis in the structural and functional integrity of the adult forebrain","volume":"11","author":"Imayoshi","year":"2008","journal-title":"Nat. Neurosci."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"a018846","DOI":"10.1101\/cshperspect.a018846","article-title":"Noncanonical Sites of Adult Neurogenesis in the Mammalian Brain","volume":"7","author":"Feliciano","year":"2015","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1038\/nrn2147","article-title":"How widespread is adult neurogenesis in mammals?","volume":"8","author":"Gould","year":"2007","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1001","DOI":"10.1242\/dev.110.4.1001","article-title":"Stem cells: Attributes, cycles, spirals, pitfalls and uncertainties. Lessons for and from the crypt","volume":"110","author":"Potten","year":"1990","journal-title":"Development"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1016\/j.brainres.2009.12.092","article-title":"Evaluation of 5-ethynyl-2\u2032-deoxyuridine staining as a sensitive and reliable method for studying cell proliferation in the adult nervous system","volume":"1319","author":"Zeng","year":"2010","journal-title":"Brain Res."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_37","first-page":"10","article-title":"Using the Student\u2019s t-test with extremely small sample sizes. Practical Assessment","volume":"18","year":"2013","journal-title":"Res. Evaluat."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/7\/3449\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T13:25:19Z","timestamp":1760361919000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/7\/3449"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,3,26]]},"references-count":37,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["ijms22073449"],"URL":"https:\/\/doi.org\/10.3390\/ijms22073449","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,3,26]]}}}