{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:35:57Z","timestamp":1790634957542,"version":"4.1.0"},"reference-count":78,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T00:00:00Z","timestamp":1616889600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100006108","name":"National Center for Advancing Translational Sciences","doi-asserted-by":"publisher","award":["UL1TR000117"],"award-info":[{"award-number":["UL1TR000117"]}],"id":[{"id":"10.13039\/100006108","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["P20GM103434"],"award-info":[{"award-number":["P20GM103434"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100001797","name":"Pharmaceutical Research and Manufacturers of America Foundation","doi-asserted-by":"publisher","award":["218218"],"award-info":[{"award-number":["218218"]}],"id":[{"id":"10.13039\/100001797","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Activation of trimethylation of histone 3 lysine 27 (H3K27me3) by EZH2, a component of the Polycomb repressive complex 2 (PRC2), is suggested to play a role in endometriosis. However, the mechanism by which this complex is dysregulated in endometriosis is not completely understood. Here, using eutopic and ectopic tissues, as well as peritoneal fluid (PF) from IRB-approved and consented patients with and without endometriosis, the expression of PRC2 complex components, JARID2, miR-155 (known regulators of EZH2), and a key inflammatory modulator, FOXP3, was measured. A higher expression of EZH2, H3K27me3, JARID2, and FOXP3 as well as miR-155 was noted in both the patient tissues and in endometrial PF treated cells. Gain-or-loss of function of miR-155 showed an effect on the PRC2 complex but had little effect on JARID2 expression, suggesting alternate pathways. Chromatin immunoprecipitation followed by qPCR showed differential expression of PRC2 complex proteins and its associated binding partners in JARID2 vs. EZH2 pull down assays. In particular, endometriotic PF treatment increased the expression of PHF19 (p = 0.0474), a gene silencer and co-factor that promotes PRC2 interaction with its targets. Thus, these studies have identified the potential novel crosstalk between miR-155-PRC2 complex-JARID2 and PHF19 in endometriosis, providing an opportunity to test other epigenetic targets in endometriosis.<\/jats:p>","DOI":"10.3390\/ijms22073492","type":"journal-article","created":{"date-parts":[[2021,3,28]],"date-time":"2021-03-28T22:09:01Z","timestamp":1616969341000},"page":"3492","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Peritoneal Modulators of EZH2-miR-155 Cross-Talk in Endometriosis"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7850-1807","authenticated-orcid":false,"given":"Sarah","family":"Brunty","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristeena","family":"Ray Wright","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenda","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3343-6081","authenticated-orcid":false,"given":"Nalini","family":"Santanam","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, Huntington, WV 25755, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,3,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1016\/j.fertnstert.2012.06.029","article-title":"Pathogenesis and pathophysiology of endometriosis","volume":"98","author":"Burney","year":"2012","journal-title":"Fertil. Steril."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2389","DOI":"10.1056\/NEJMcp1000274","article-title":"Clinical practice. Endometriosis","volume":"362","author":"Giudice","year":"2010","journal-title":"N. Engl. J. Med."},{"key":"ref_3","first-page":"656571","article-title":"Pathogenesis of endometriosis and uterine fibroids","volume":"2013","author":"Ciarmela","year":"2013","journal-title":"Obs. Gynecol. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1097\/GCO.0000000000000084","article-title":"Novel agents for the medical treatment of endometriosis","volume":"26","author":"Platteeuw","year":"2014","journal-title":"Curr. Opin. Obstet. Gynecol."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Rowlands, I.J., Abbott, J.A., Montgomery, G.W., Hockey, R., Rogers, P., and Mishra, G.D. (2020). Prevalence and incidence of endometriosis in Australian women: A data linkage cohort study. BJOG.","DOI":"10.1111\/1471-0528.16447"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1093\/molehr\/gap064","article-title":"Epigenetics of endometriosis","volume":"15","author":"Guo","year":"2009","journal-title":"Mol. Hum. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"683","DOI":"10.1111\/j.1447-0756.2011.01663.x","article-title":"Aberrant DNA methylation status of endometriosis: Epigenetics as the pathogenesis, biomarker and therapeutic target","volume":"37","author":"Nasu","year":"2011","journal-title":"J. Obstet. Gynaecol. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1134","DOI":"10.1177\/1933719115578924","article-title":"H3K27me3 is an Epigenetic Mark of Relevance in Endometriosis","volume":"22","author":"Monteiro","year":"2015","journal-title":"Reprod. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1089\/acm.2012.0623","article-title":"Western herbal medicine, epigenetics, and endometriosis","volume":"19","author":"Stephens","year":"2013","journal-title":"J. Altern. Complem. Med."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Brunty, S., Mitcell, B., Bou-Zgheib, N., and Santanam, N. (2020). Endometriosis and ovarian cancer risk, an epigenetic connection. Ann. Transl. Med.","DOI":"10.21037\/atm-20-2449"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1159\/000052856","article-title":"Pathogenesis of endometriosis: The role of peritoneal fluid","volume":"47","author":"Koninckx","year":"1999","journal-title":"Gynecol. Obs. Investig."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/s41574-019-0191-9","article-title":"Genesis, genes and epigenetics of endometriosis-associated infertility","volume":"15","author":"Guo","year":"2019","journal-title":"Nat. Rev. Endocrinol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"396","DOI":"10.1038\/nature05913","article-title":"Perceptions of epigenetics","volume":"447","author":"Bird","year":"2007","journal-title":"Nature"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"249","DOI":"10.1016\/j.ydbio.2016.06.005","article-title":"Epigenetics: The origins and evolution of a fashionable topic","volume":"416","author":"Deichmann","year":"2016","journal-title":"Dev. Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"340","DOI":"10.1038\/nrm3789","article-title":"What are memories made of? How Polycomb and Trithorax proteins mediate epigenetic memory","volume":"15","author":"Steffen","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1039","DOI":"10.1126\/science.1076997","article-title":"Role of histone H3 lysine 27 methylation in Polycomb-group silencing","volume":"298","author":"Cao","year":"2002","journal-title":"Science"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"2893","DOI":"10.1101\/gad.1035902","article-title":"Histone methyltransferase activity associated with a human multiprotein complex containing the Enhancer of Zeste protein","volume":"16","author":"Kuzmichev","year":"2002","journal-title":"Genes Dev."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2876","DOI":"10.1242\/dev.120030","article-title":"Trithorax and Polycomb group-dependent regulation: A tale of opposing activities","volume":"142","author":"Geisler","year":"2015","journal-title":"Development"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.gde.2005.08.002","article-title":"DNA methylation and histone modifications: Teaming up to silence genes","volume":"15","author":"Fuks","year":"2005","journal-title":"Curr. Opin. Genet. Dev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"871","DOI":"10.1038\/nature04431","article-title":"The Polycomb group protein EZH2 directly controls DNA methylation","volume":"439","author":"Vire","year":"2006","journal-title":"Nature"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"455","DOI":"10.3349\/ymj.2009.50.4.455","article-title":"Epigenetic cross-talk between DNA methylation and histone modifications in human cancers","volume":"50","author":"Kondo","year":"2009","journal-title":"Yonsei Med. J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1093\/biolre\/ioy030","article-title":"Effects of histone methyltrasnferase inhibition in endometriosis","volume":"99","author":"Young","year":"2018","journal-title":"Biol. Reprod."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1812","DOI":"10.1007\/s43032-020-00257-9","article-title":"Inhibition of Histone Methyltransferase EZH2 Suppresses Endometriotic Vesicle Development in a Rat Model of Endometriosis","volume":"27","author":"Luna","year":"2020","journal-title":"Reprod. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1016\/j.mce.2015.03.023","article-title":"Selective inhibition of prostaglandin E2 receptors EP2 and EP4 modulates DNA methylation and histone modification machinery proteins in human endometriotic cells","volume":"409","author":"Arosh","year":"2015","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1101\/gad.1886410","article-title":"Jarid2 and PRC2, partners in regulating gene expression","volume":"24","author":"Li","year":"2010","journal-title":"Genes Dev."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"715","DOI":"10.1038\/nrg1945","article-title":"JmjC-domain-containing proteins and histone demethylation","volume":"7","author":"Klose","year":"2006","journal-title":"Nat. Rev. Genet."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1038\/nrm3327","article-title":"Molecular mechanisms and potential functions of histone demethylases","volume":"13","author":"Kooistra","year":"2012","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5587","DOI":"10.1158\/0008-5472.CAN-19-0428","article-title":"An Allosteric PRC2 Inhibitor Targeting EED Suppresses Tumor Progression by Modulating the Immune Response","volume":"79","author":"Dong","year":"2019","journal-title":"Cancer Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.molcel.2014.12.020","article-title":"Jarid2 Methylation via the PRC2 Complex Regulates H3K27me3 Deposition during Cell Differentiation","volume":"57","author":"Sanulli","year":"2015","journal-title":"Mol. Cell"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1038\/nature08788","article-title":"JARID2 regulates binding of the Polycomb repressive complex 2 to target genes in ES cells","volume":"464","author":"Pasini","year":"2010","journal-title":"Nature"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1016\/j.tcb.2010.10.004","article-title":"Inactive yet indispensable: The tale of Jarid2","volume":"21","author":"Landeira","year":"2011","journal-title":"Trends Cell Biol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.freeradbiomed.2015.01.028","article-title":"Oxidative stress alters global histone modification and DNA methylation","volume":"82","author":"Niu","year":"2015","journal-title":"Free Radic. Biol. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"956","DOI":"10.1016\/j.redox.2017.04.037","article-title":"Redox regulation of microRNAs in endometriosis-associated pain","volume":"12","author":"Wright","year":"2017","journal-title":"Redox Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1186\/1476-4598-13-79","article-title":"MicroRNA-155 as an inducer of apoptosis and cell differentiation in Acute Myeloid Leukaemia","volume":"13","author":"Palma","year":"2014","journal-title":"Mol. Cancer"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"865","DOI":"10.1016\/j.immuni.2014.03.014","article-title":"miR-155 activates cytokine gene expression in Th17 cells by regulating the DNA-binding protein Jarid2 to relieve polycomb-mediated repression","volume":"40","author":"Escobar","year":"2014","journal-title":"Immunity"},{"key":"ref_36","first-page":"4545","article-title":"Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155","volume":"8","author":"Yao","year":"2015","journal-title":"Int. J. Clin. Exp. Pathol."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Jablonski, K.A., Gaudet, A.D., Amici, S.A., Popovich, P.G., and Guerau-de-Arellano, M. (2016). Control of the Inflammatory Macrophage Transcriptional Signature by miR-155. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0159724"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"2578","DOI":"10.4049\/jimmunol.0803162","article-title":"Cutting edge: The Foxp3 target miR-155 contributes to the development of regulatory T cells","volume":"182","author":"Kohlhaas","year":"2009","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.jri.2010.11.004","article-title":"Regulatory T cells and other leukocytes in the pathogenesis of endometriosis","volume":"88","author":"Berbic","year":"2011","journal-title":"J. Reprod. Immunol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1111\/j.1600-0897.2012.01173.x","article-title":"CD4(+) CD25(high) Foxp3(+) cells increased in the peritoneal fluid of patients with endometriosis","volume":"68","author":"Podgaec","year":"2012","journal-title":"Am. J. Reprod. Immunol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1093\/humrep\/des346","article-title":"CD4(+) CD25(+) FOXP3(+) regulatory T cells in peripheral blood and peritoneal fluid of patients with endometriosis","volume":"28","author":"Bocian","year":"2013","journal-title":"Hum. Reprod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"27708","DOI":"10.18632\/oncotarget.25523","article-title":"FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1","volume":"9","author":"Brown","year":"2018","journal-title":"Oncotarget"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1016\/j.bbamcr.2013.05.014","article-title":"Downregulation of Ezh2 methyltransferase by FOXP3: New insight of FOXP3 into chromatin remodeling?","volume":"1833","author":"Shen","year":"2013","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"34372","DOI":"10.1074\/jbc.M111.325332","article-title":"Polycomb antagonizes p300\/CREB-binding protein-associated factor to silence FOXP3 in a Kruppel-like factor-dependent manner","volume":"287","author":"Xiong","year":"2012","journal-title":"J. Biol. Chem."},{"key":"ref_45","first-page":"595","article-title":"The peritoneum\u2014An important factor for pathogenesis and pain generation in endometriosis","volume":"92","author":"Mechsner","year":"2014","journal-title":"J. Mol. Med."},{"key":"ref_46","unstructured":"Feingold, K.R., Anawalt, B., Boyce, A., Chrousos, G., de Herder, W.W., Dungan, K., Grossman, A., Hershman, J.M., Hofland, H.J., and Kaltsas, G. (2000). Endometriosis. Endotext, MDText.com.Inc."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1093\/humrep\/17.2.426","article-title":"Prediction of endometriosis with serum and peritoneal fluid markers: A prospective controlled trial","volume":"17","author":"Bedaiwy","year":"2002","journal-title":"Hum. Reprod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1191","DOI":"10.1016\/j.fertnstert.2017.03.013","article-title":"Peritoneal fluid cytokines related to endometriosis in patients evaluated for infertility","volume":"107","author":"Jorgensen","year":"2017","journal-title":"Fertil. Steril."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2292","DOI":"10.1093\/humrep\/dev204","article-title":"Peritoneal fluid modifies the microRNA expression profile in endometrial and endometriotic cells from women with endometriosis","volume":"30","author":"Arroyo","year":"2015","journal-title":"Hum. Reprod."},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Braza-Boils, A., Gilabert-Estelles, J., Ramon, L.A., Gilabert, J., Mari-Alexandre, J., Chirivella, M., Espana, F., and Estelles, A. (2013). Peritoneal fluid reduces angiogenesis-related microRNA expression in cell cultures of endometrial and endometriotic tissues from women with endometriosis. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0062370"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e1032476","DOI":"10.1080\/23723556.2015.1032476","article-title":"Epigenetic synthetic lethality in ovarian clear cell carcinoma: EZH2 and ARID1A mutations","volume":"3","author":"Bitler","year":"2016","journal-title":"Mol. Cell. Oncol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"e58675","DOI":"10.7554\/eLife.58675","article-title":"Structural basis for histone variant H3tK27me3 recognition by PHF1 and PHF19","volume":"9","author":"Dong","year":"2020","journal-title":"Elife"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"287","DOI":"10.1038\/nature23881","article-title":"Polycomb-like proteins link the PRC2 complex to CpG islands","volume":"549","author":"Li","year":"2017","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"2157","DOI":"10.1038\/s41467-019-09882-8","article-title":"miR-155 harnesses Phf19 to potentiate cancer immunotherapy through epigenetic reprogramming of CD8(+) T cell fate","volume":"10","author":"Ji","year":"2019","journal-title":"Nat. Commun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1097\/01.j.pain.0000460321.72396.88","article-title":"Oxidation-sensitive nociception involved in endometriosis-associated pain","volume":"156","author":"Ray","year":"2015","journal-title":"Pain"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"163","DOI":"10.5301\/je.5000199","article-title":"Power over pain: A brief review of current and novel interventions for endometriosis-associated pain","volume":"6","author":"Ray","year":"2014","journal-title":"J. Endometr. Pelvic Pain Disord."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/S0015-0282(02)03333-2","article-title":"Induction of monocyte chemotactic protein-1 in peritoneal mesothelial and endometrial cells by oxidized low-density lipoprotein and peritoneal fluid from women with endometriosis","volume":"78","author":"Rong","year":"2002","journal-title":"Fertil. Steril."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"189","DOI":"10.1016\/j.trsl.2012.05.001","article-title":"Antioxidant supplementation reduces endometriosis-related pelvic pain in humans","volume":"161","author":"Santanam","year":"2013","journal-title":"Transl. Res."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1711","DOI":"10.3892\/ol.2017.6345","article-title":"Preliminary study on decreasing the expression of FOXP3 with miR-155 to inhibit diffuse large B-cell lymphoma","volume":"14","author":"Zhang","year":"2017","journal-title":"Oncol. Lett."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"G989","DOI":"10.1152\/ajpgi.00032.2016","article-title":"Foxp3 promoter methylation impairs suppressive function of regulatory T cells in biliary atresia","volume":"311","author":"Li","year":"2016","journal-title":"Am. J. Physiol. Gastrointest. Liver Physiol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1705","DOI":"10.1007\/s10753-019-01030-0","article-title":"The Hypermethylation of Foxp3 Promoter Impairs the Function of Treg Cells in EAP","volume":"42","author":"Chen","year":"2019","journal-title":"Inflammation"},{"key":"ref_62","first-page":"55","article-title":"Disruption of FOXP3-EZH2 Interaction Represents a Pathobiological Mechanism in Intestinal Inflammation","volume":"7","author":"Bamidele","year":"2019","journal-title":"Cell. Mol. Gastroenterol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"3801","DOI":"10.12659\/MSM.905226","article-title":"Role of Interleukin-6 and Its Receptor in Endometriosis","volume":"23","author":"Li","year":"2017","journal-title":"Med. Sci. Monit."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1016\/S0092-8674(02)00976-5","article-title":"Histone methyltransferase activity of a Drosophila Polycomb group repressor complex","volume":"111","author":"Muller","year":"2002","journal-title":"Cell"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1172","DOI":"10.1182\/blood-2015-03-634428","article-title":"Ezh2 loss in hematopoietic stem cells predisposes mice to develop heterogeneous malignancies in an Ezh1-dependent manner","volume":"126","author":"Aoyama","year":"2015","journal-title":"Blood"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1016\/j.molcel.2008.10.016","article-title":"EZH1 mediates methylation on histone H3 lysine 27 and complements EZH2 in maintaining stem cell identity and executing pluripotency","volume":"32","author":"Shen","year":"2008","journal-title":"Mol. Cell"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"2663","DOI":"10.1101\/gad.225888.113","article-title":"Nucleosome-binding activities within JARID2 and EZH1 regulate the function of PRC2 on chromatin","volume":"27","author":"Son","year":"2013","journal-title":"Genes Dev."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1016\/j.bbrc.2012.11.116","article-title":"Tudor domains of the PRC2 components PHF1 and PHF19 selectively bind to histone H3K36me3","volume":"430","author":"Qin","year":"2013","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1273","DOI":"10.1038\/nsmb.2449","article-title":"Polycomb PHF19 binds H3K36me3 and recruits PRC2 and demethylase NO66 to embryonic stem cell genes during differentiation","volume":"19","author":"Brien","year":"2012","journal-title":"Nat. Struct. Mol. Biol."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1182\/blood.2019000578","article-title":"PHF19 promotes multiple myeloma tumorigenicity through PRC2 activation and broad H3K27me3 domain formation","volume":"134","author":"Ren","year":"2019","journal-title":"Blood"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1634","DOI":"10.4161\/cc.20095","article-title":"PHF19 and Akt control the switch between proliferative and invasive states in melanoma","volume":"11","author":"Ghislin","year":"2012","journal-title":"Cell Cycle"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"A28","DOI":"10.1158\/1538-7445.CEC13-A28","article-title":"Abstract A28: Targeting PCL3\/PHF19 as an alternative therapeutic strategy to EZH2 inhibition in PRC2-deregulated cancers","volume":"73","author":"Brien","year":"2013","journal-title":"Cancer Res."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1038\/s41389-019-0122-6","article-title":"Dihydroartemisinin inhibits prostate cancer via JARID2\/miR-7\/miR-34a-dependent downregulation of Axl","volume":"8","author":"Paccez","year":"2019","journal-title":"Oncogenesis"},{"key":"ref_74","first-page":"1103","article-title":"Establishment of a new human endometrial adenocarcinoma cell line, Ishikawa cells, containing estrogen and progesterone receptors","volume":"37","author":"Nishida","year":"1985","journal-title":"Nihon Sanka Fujinka Gakkai Zasshi"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1016\/j.mce.2005.11.041","article-title":"Progesterone resistance in endometriosis: Link to failure to metabolize estradiol","volume":"248","author":"Bulun","year":"2006","journal-title":"Mol. Cell. Endocrinol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1016\/j.fertnstert.2016.05.020","article-title":"Aromatase inhibitor regulates let-7 expression and let-7f-induced cell migration in endometrial cells from women with endometriosis","volume":"106","author":"Cho","year":"2016","journal-title":"Fertil. Steril."},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","article-title":"Protein measurement with the Folin phenol reagent","volume":"193","author":"Lowry","year":"1951","journal-title":"J. Biol. Chem."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1007\/978-1-4939-2694-7_47","article-title":"Protein detection by Simple Western analysis","volume":"1312","author":"Harris","year":"2015","journal-title":"Methods Mol. 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