{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T20:42:01Z","timestamp":1781901721362,"version":"3.54.5"},"reference-count":127,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T00:00:00Z","timestamp":1617667200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CHU de Qu\u00e9bec Research Center","award":["N\/A"],"award-info":[{"award-number":["N\/A"]}]},{"DOI":"10.13039\/501100000156","name":"Fonds de Recherche du Qu\u00e9bec - Sant\u00e9","doi-asserted-by":"publisher","award":["262093"],"award-info":[{"award-number":["262093"]}],"id":[{"id":"10.13039\/501100000156","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003151","name":"Fonds de recherche du Qu\u00e9bec \u2013 Nature et technologies","doi-asserted-by":"publisher","award":["289637"],"award-info":[{"award-number":["289637"]}],"id":[{"id":"10.13039\/501100003151","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ebola virus (EBOV) is a virulent pathogen, notorious for inducing life-threatening hemorrhagic fever, that has been responsible for several outbreaks in Africa and remains a public health threat. Yet, its pathogenesis is still not completely understood. Although there have been numerous studies on host transcriptional response to EBOV, with an emphasis on the clinical features, the impact of EBOV infection on post-transcriptional regulatory elements, such as microRNAs (miRNAs), remains largely unexplored. MiRNAs are involved in inflammation and immunity and are believed to be important modulators of the host response to viral infection. Here, we have used small RNA sequencing (sRNA-Seq), qPCR and functional analyses to obtain the first comparative miRNA transcriptome (miRNome) of a human liver cell line (Huh7) infected with one of the following three EBOV strains: Mayinga (responsible for the first Zaire outbreak in 1976), Makona (responsible for the West Africa outbreak in 2013\u20132016) and the epizootic Reston (presumably innocuous to humans). Our results highlight specific miRNA-based immunity pathways and substantial differences between the strains beyond their clinical manifestation and pathogenicity. These analyses shed new light into the molecular signature of liver cells upon EBOV infection and reveal new insights into miRNA-based virus attack and host defense strategy.<\/jats:p>","DOI":"10.3390\/ijms22073792","type":"journal-article","created":{"date-parts":[[2021,4,6]],"date-time":"2021-04-06T10:34:12Z","timestamp":1617705252000},"page":"3792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Altered microRNA Transcriptome in Cultured Human Liver Cells upon Infection with Ebola Virus"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6259-5850","authenticated-orcid":false,"given":"Idrissa","family":"Diallo","sequence":"first","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9983-0113","authenticated-orcid":false,"given":"Jeffrey","family":"Ho","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Laffont","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Laugier","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9504-3120","authenticated-orcid":false,"given":"Abderrahim","family":"Benmoussa","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3749-920X","authenticated-orcid":false,"given":"Marine","family":"Lambert","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeinab","family":"Husseini","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geoff","family":"Soule","sequence":"additional","affiliation":[{"name":"Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3B 3M9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Kozak","sequence":"additional","affiliation":[{"name":"Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3B 3M9, Canada"},{"name":"Division of Microbiology, Department of Laboratory Medicine &amp; Molecular Diagnostics, Sunnybrook Health Sciences Centre, Toronto, ON M4N 3M5, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary P.","family":"Kobinger","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"},{"name":"Special Pathogens Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3B 3M9, Canada"},{"name":"D\u00e9partement de Microbiologie M\u00e9dicale, Universit\u00e9 du Manitoba, Winnipeg, MB R3E 0J9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6099-6562","authenticated-orcid":false,"given":"Patrick","family":"Provost","sequence":"additional","affiliation":[{"name":"CHU de Qu\u00e9bec Research Center, Department of Microbiology, Infectious Diseases and Immunology, Faculty of Medicine, Universit\u00e9 Laval, Quebec, QC G1V 4G2, Canada"},{"name":"CHUQ Research Center\/CHUL Pavilion, 2705 Blvd Laurier, Room T1-65, Quebec, QC G1V 4G2, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,6]]},"reference":[{"key":"ref_1","unstructured":"CDC (2020, December 06). 2014\u20132016 Ebola Outbreak in West Africa|History|Ebola (Ebola Virus Disease)|CDC, Available online: https:\/\/www.cdc.gov\/vhf\/ebola\/history\/2014-2016-outbreak\/index.html."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Coltart, C.E.M., Lindsey, B., Ghinai, I., Johnson, A.M., and Heymann, D.L. (2017). The Ebola Outbreak, 2013\u20132016: Old Lessons for New Epidemics. Philos. Trans. R. Soc. Lond. B Biol. Sci., 372.","DOI":"10.1098\/rstb.2016.0297"},{"key":"ref_3","first-page":"1229","article-title":"Virus Nomenclature below the Species Level: A Standardized Nomenclature for Filovirus Strains and Variants Rescued from CDNA","volume":"159","author":"Kuhn","year":"2014","journal-title":"Arch. Virol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Cantoni, D., Hamlet, A., Michaelis, M., Wass, M.N., and Rossman, J.S. (2016). Risks Posed by Reston, the Forgotten Ebolavirus. mSphere, 1.","DOI":"10.1128\/mSphere.00322-16"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1084","DOI":"10.1038\/s41564-018-0227-2","article-title":"Discovery of a New Ebolavirus (Bombali Virus) in Molossid Bats in Sierra Leone","volume":"3","author":"Goldstein","year":"2018","journal-title":"Nat. Microbiol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"102375","DOI":"10.1016\/j.jaut.2019.102375","article-title":"Ebola Virus Disease: An Emerging and Re-Emerging Viral Threat","volume":"106","author":"Rojas","year":"2020","journal-title":"J. Autoimmun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1189\/jlb.4RI0316-099RR","article-title":"Molecular Mechanisms of Ebola Pathogenesis","volume":"100","author":"Rivera","year":"2016","journal-title":"J. Leukoc. Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"S957","DOI":"10.1093\/infdis\/jir326","article-title":"Ebola Virus Enters Host Cells by Macropinocytosis and Clathrin-Mediated Endocytosis","volume":"204","author":"Aleksandrowicz","year":"2011","journal-title":"J. Infect. Dis."},{"key":"ref_9","first-page":"323","article-title":"Mechanisms of Filovirus Entry","volume":"411","author":"Davey","year":"2017","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2347","DOI":"10.1016\/S0002-9440(10)63591-2","article-title":"Pathogenesis of Ebola Hemorrhagic Fever in Cynomolgus Macaques: Evidence That Dendritic Cells Are Early and Sustained Targets of Infection","volume":"163","author":"Geisbert","year":"2003","journal-title":"Am. J. Pathol."},{"key":"ref_11","first-page":"39","article-title":"Ebola Haemorrhagic Fever Virus: Pathogenesis, Immune Responses, Potential Prevention","volume":"54","author":"Marcinkiewicz","year":"2014","journal-title":"Folia Med. Cracov."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1586\/1744666X.2014.908705","article-title":"Characterization of Host Immune Responses in Ebola Virus Infections","volume":"10","author":"Wong","year":"2014","journal-title":"Expert Rev. Clin. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"122","DOI":"10.1016\/j.virol.2015.03.030","article-title":"Innate Immune Evasion by Filoviruses","volume":"479\u2013480","author":"Basler","year":"2015","journal-title":"Virology"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1146\/annurev-pathol-052016-100506","article-title":"The Pathogenesis of Ebola Virus Disease","volume":"12","author":"Baseler","year":"2017","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"13469","DOI":"10.1128\/JVI.01097-07","article-title":"Ebola Virus VP24 Proteins Inhibit the Interaction of NPI-1 Subfamily Karyopherin Alpha Proteins with Activated STAT1","volume":"81","author":"Reid","year":"2007","journal-title":"J. Virol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1073\/pnas.0807854106","article-title":"Structure of the Ebola VP35 Interferon Inhibitory Domain","volume":"106","author":"Leung","year":"2009","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","first-page":"293","article-title":"Filovirus Strategies to Escape Antiviral Responses","volume":"411","author":"Olejnik","year":"2017","journal-title":"Curr. Top. Microbiol. Immunol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.cell.2018.03.006","article-title":"Metazoan MicroRNAs","volume":"173","author":"Bartel","year":"2018","journal-title":"Cell"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most Mammalian MRNAs Are Conserved Targets of MicroRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1038\/leu.2011.356","article-title":"MicroRNAs in Inflammation and Immune Responses","volume":"26","author":"Contreras","year":"2012","journal-title":"Leukemia"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"279","DOI":"10.1038\/nri.2016.40","article-title":"MicroRNAs as Regulatory Elements in Immune System Logic","volume":"16","author":"Mehta","year":"2016","journal-title":"Nat. Rev. Immunol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wang, Z., Li, J., Fu, Y., Zhao, Z., Zhang, C., Li, N., Li, J., Cheng, H., Jin, X., and Lu, B. (2018). A Rapid Screen for Host-Encoded MiRNAs with Inhibitory Effects against Ebola Virus Using a Transcription- and Replication-Competent Virus-Like Particle System. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19051488"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1007\/s11427-014-4742-y","article-title":"Hsa-MiR-1246, Hsa-MiR-320a and Hsa-MiR-196b-5p Inhibitors Can Reduce the Cytotoxicity of Ebola Virus Glycoprotein in vitro","volume":"57","author":"Sheng","year":"2014","journal-title":"Sci. China Life Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Duy, J., Koehler, J.W., Honko, A.N., Schoepp, R.J., Wauquier, N., Gonzalez, J.-P., Pitt, M.L., Mucker, E.M., Johnson, J.C., and O\u2019Hearn, A. (2016). Circulating MicroRNA Profiles of Ebola Virus Infection. Sci. Rep., 6.","DOI":"10.1038\/srep24496"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Oliver, G.F., Orang, A.V., Appukuttan, B., Marri, S., Michael, M.Z., Marsh, G.A., and Smith, J.R. (2019). Expression of MicroRNA in Human Retinal Pigment Epithelial Cells Following Infection with Zaire Ebolavirus. BMC Res. Notes, 12.","DOI":"10.1186\/s13104-019-4671-8"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"626","DOI":"10.3855\/jidc.7127","article-title":"Inhibition of Ebola Virus by Anti-Ebola MiRNAs in Silico","volume":"10","author":"Golkar","year":"2016","journal-title":"J. Infect. Dev. Ctries."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1016\/j.virol.2011.01.002","article-title":"Virus-Encoded MicroRNAs","volume":"411","author":"Grundhoff","year":"2011","journal-title":"Virology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"6480","DOI":"10.1038\/s41598-018-23916-z","article-title":"Virus-Encoded MiRNAs in Ebola Virus Disease","volume":"8","author":"Duy","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"9912","DOI":"10.1038\/srep09912","article-title":"Systematic Genome-Wide Screening and Prediction of MicroRNAs in EBOV During the 2014 Ebolavirus Outbreak","volume":"5","author":"Teng","year":"2015","journal-title":"Sci. Rep."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Bosworth, A., Dowall, S.D., Armstrong, S., Liu, X., Dong, X., Bruce, C.B., Ng, L.F.P., Carroll, M.W., Hewson, R., and Hiscox, J.A. (2019). Investigating the Cellular Transcriptomic Response Induced by the Makona Variant of Ebola Virus in Differentiated THP-1 Cells. Viruses, 11.","DOI":"10.3390\/v11111023"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Marzi, A., Feldmann, F., Hanley, P.W., Scott, D., G\u00fcnther, S., and Feldmann, H. (2015). Delayed Disease Progression in Cynomolgus Macaques Infected with Ebola Virus Makona Strain. Emerg. Infect. Dis. J. CDC, 21.","DOI":"10.3201\/eid2110.150259"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"43144","DOI":"10.1038\/srep43144","article-title":"A Comparison of Host Gene Expression Signatures Associated with Infection in Vitro by the Makona and Ecran (Mayinga) Variants of Ebola Virus","volume":"7","author":"Bosworth","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3009","DOI":"10.1128\/JVI.80.6.3009-3020.2006","article-title":"Global Suppression of the Host Antiviral Response by Ebola- and Marburgviruses: Increased Antagonism of the Type I Interferon Response is Associated with Enhanced Virulence","volume":"80","author":"Kash","year":"2006","journal-title":"J. Virol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"265","DOI":"10.12688\/f1000research.5666.2","article-title":"Correlating the Ability of VP24 Protein from Ebola and Marburg Viruses to Bind Human Karyopherin to Their Immune Suppression Mechanism and Pathogenicity Using Computational Methods","volume":"3","author":"Chakraborty","year":"2014","journal-title":"F1000Research"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"764","DOI":"10.1093\/femsre\/fuv031","article-title":"Ebolavirus Comparative Genomics","volume":"39","author":"Jun","year":"2015","journal-title":"Fems. Microbiol. Rev."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1418","DOI":"10.1056\/NEJMoa1404505","article-title":"Emergence of Zaire Ebola Virus Disease in Guinea","volume":"371","author":"Baize","year":"2014","journal-title":"N. Engl. J. Med."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Olejnik, J., Forero, A., Deflub\u00e9, L.R., Hume, A.J., Manhart, W.A., Nishida, A., Marzi, A., Katze, M.G., Ebihara, H., and Rasmussen, A.L. (2017). Ebolaviruses Associated with Differential Pathogenicity Induce Distinct Host Responses in Human Macrophages. J. Virol., 91.","DOI":"10.1128\/JVI.00179-17"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1136\/jcp.31.3.201","article-title":"Ultrastructure of Ebola Virus Particles in Human Liver","volume":"31","author":"Ellis","year":"1978","journal-title":"J. Clin. Pathol."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Liu, X., Speranza, E., Mu\u00f1oz-Fontela, C., Haldenby, S., Rickett, N.Y., Garcia-Dorival, I., Fang, Y., Hall, Y., Zekeng, E.-G., and L\u00fcdtke, A. (2017). Transcriptomic Signatures Differentiate Survival from Fatal Outcomes in Humans Infected with Ebola Virus. Genome Biol., 18.","DOI":"10.1186\/s13059-016-1137-3"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1002\/path.4456","article-title":"Tissue and Cellular Tropism, Pathology and Pathogenesis of Ebola and Marburg Viruses","volume":"235","author":"Martines","year":"2015","journal-title":"J. Pathol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Logue, J., Vargas Licona, W., Cooper, T.K., Reeder, B., Byrum, R., Qin, J., Deiuliis Murphy, N., Cong, Y., Bonilla, A., and Sword, J. (2019). Ebola Virus Isolation Using Huh-7 Cells Has Methodological Advantages and Similar Sensitivity to Isolation Using Other Cell Types and Suckling BALB\/c Laboratory Mice. Viruses, 11.","DOI":"10.3390\/v11020161"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Jankeel, A., Menicucci, A.R., Woolsey, C., Fenton, K.A., Mendoza, N., Versteeg, K., Cross, R.W., Geisbert, T.W., and Messaoudi, I. (2020). Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques. J. Virol., 94.","DOI":"10.1128\/JVI.00250-20"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5348","DOI":"10.1128\/JVI.00215-08","article-title":"Whole-Genome Expression Profiling Reveals That Inhibition of Host Innate Immune Response Pathways by Ebola Virus Can Be Reversed by a Single Amino Acid Change in the VP35 Protein","volume":"82","author":"Hartman","year":"2008","journal-title":"J. Virol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"849","DOI":"10.1016\/S0140-6736(10)60667-8","article-title":"Ebola Haemorrhagic Fever","volume":"377","author":"Feldmann","year":"2011","journal-title":"Lancet"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1136\/jcp.43.10.813","article-title":"Use of Immunoelectron Microscopy to Show Ebola Virus during the 1989 United States Epizootic","volume":"43","author":"Geisbert","year":"1990","journal-title":"J. Clin. Pathol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"9060","DOI":"10.1128\/JVI.00659-11","article-title":"Functional Genomics Reveals the Induction of Inflammatory Response and Metalloproteinase Gene Expression during Lethal Ebola Virus Infection","volume":"85","author":"Cilloniz","year":"2011","journal-title":"J. Virol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1618","DOI":"10.1086\/379724","article-title":"Mechanisms Underlying Coagulation Abnormalities in Ebola Hemorrhagic Fever: Overexpression of Tissue Factor in Primate Monocytes\/Macrophages Is a Key Event","volume":"188","author":"Geisbert","year":"2003","journal-title":"J. Infect. Dis."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"309","DOI":"10.1007\/978-1-4939-2074-7_23","article-title":"Measurement of Blood Coagulation Factor Synthesis in Cultures of Human Hepatocytes","volume":"1250","author":"Heinz","year":"2015","journal-title":"Methods Mol. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Gene Ontology Consortium (2019). The Gene Ontology Resource: 20 Years and Still Going Strong. Nucleic Acids Res., 47, D330\u2013D338.","DOI":"10.1093\/nar\/gky1055"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"25","DOI":"10.1038\/75556","article-title":"Gene Ontology: Tool for the Unification of Biology","volume":"25","author":"Ashburner","year":"2000","journal-title":"Nat. Genet."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1093\/nar\/28.1.27","article-title":"KEGG: Kyoto Encyclopedia of Genes and Genomes","volume":"28","author":"Kanehisa","year":"2000","journal-title":"Nucleic Acids Res."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"1149","DOI":"10.2217\/fon.11.95","article-title":"Targeting the PI3K\/Akt\/MTOR Pathway in Hepatocellular Carcinoma","volume":"7","author":"Zhou","year":"2011","journal-title":"Future Oncol."},{"key":"ref_53","first-page":"3060","article-title":"Tropomodulin 3 Promotes Liver Cancer Progression by Activating the MAPK\/ERK Signaling Pathway","volume":"41","author":"Jin","year":"2019","journal-title":"Oncol. Rep."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Sequera, C., Manzano, S., Guerrero, C., and Porras, A. (2018). How Rap and Its GEFs Control Liver Physiology and Cancer Development. C3G Alterations in Human Hepatocarcinoma. Hepat. Oncol., 5.","DOI":"10.2217\/hep-2017-0026"},{"key":"ref_55","first-page":"667","article-title":"Importance of cellular microRNAs in the regulation of viral infections","volume":"35","author":"Girardi","year":"2019","journal-title":"Med. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Girardi, E., L\u00f3pez, P., and Pfeffer, S. (2018). On the Importance of Host MicroRNAs During Viral Infection. Front. Genet., 9.","DOI":"10.3389\/fgene.2018.00439"},{"key":"ref_57","doi-asserted-by":"crossref","unstructured":"Bruscella, P., Bottini, S., Baudesson, C., Pawlotsky, J.-M., Feray, C., and Trabucchi, M. (2017). Viruses and MiRNAs: More Friends than Foes. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00824"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.1038\/s41467-018-05053-3","article-title":"MicroRNA-122 Amplifies Hepatitis C Virus Translation by Shaping the Structure of the Internal Ribosomal Entry Site","volume":"9","author":"Schult","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Pereira, A., Moreira, F., Vinasco-Sandoval, T., Cunha, A., Vidal, A., Ribeiro-dos-Santos, A.M., Pinto, P., Magalh\u00e3es, L., Assump\u00e7\u00e3o, M., and Demachki, S. (2019). MiRNome Reveals New Insights Into the Molecular Biology of Field Cancerization in Gastric Cancer. Front. Genet., 10.","DOI":"10.3389\/fgene.2019.00592"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1093\/biostatistics\/4.3.465","article-title":"The Efficiency of Pooling MRNA in Microarray Experiments","volume":"4","author":"Kendziorski","year":"2003","journal-title":"Biostatistics"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"4252","DOI":"10.1073\/pnas.0500607102","article-title":"On the Utility of Pooling Biological Samples in Microarray Experiments","volume":"102","author":"Kendziorski","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1098","DOI":"10.1271\/bbb.69.1098","article-title":"Representation of Individual Gene Expression in Completely Pooled MRNA Samples","volume":"69","author":"Glass","year":"2005","journal-title":"Biosci. Biotechnol. Biochem."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"610","DOI":"10.1101\/gr.7179508","article-title":"Application of Massively Parallel Sequencing to MicroRNA Profiling and Discovery in Human Embryonic Stem Cells","volume":"18","author":"Morin","year":"2008","journal-title":"Genome Res."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1120","DOI":"10.1261\/rna.055509.115","article-title":"Evaluation of MicroRNA Alignment Techniques","volume":"22","author":"Ziemann","year":"2016","journal-title":"RNA"},{"key":"ref_65","doi-asserted-by":"crossref","unstructured":"Lambert, M., Benmoussa, A., and Provost, P. (2019). Small Non-Coding RNAs Derived From Eukaryotic Ribosomal RNA. Noncoding RNA, 5.","DOI":"10.3390\/ncrna5010016"},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Diallo, I., and Provost, P. (2020). RNA-Sequencing Analyses of Small Bacterial RNAs and Their Emergence as Virulence Factors in Host-Pathogen Interactions. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21051627"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1016\/j.ygeno.2017.03.001","article-title":"Evaluation and Assessment of Read-Mapping by Multiple next-Generation Sequencing Aligners Based on Genome-Wide Characteristics","volume":"109","author":"Sen","year":"2017","journal-title":"Genomics"},{"key":"ref_68","doi-asserted-by":"crossref","unstructured":"Lindner, R., and Friedel, C.C. (2012). A Comprehensive Evaluation of Alignment Algorithms in the Context of RNA-Seq. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0052403"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1261\/rna.036475.112","article-title":"Differences in MicroRNA Detection Levels Are Technology and Sequence Dependent","volume":"19","author":"Leshkowitz","year":"2013","journal-title":"RNA"},{"key":"ref_70","doi-asserted-by":"crossref","unstructured":"Wauquier, N., Becquart, P., Padilla, C., Baize, S., and Leroy, E.M. (2010). Human Fatal Zaire Ebola Virus Infection Is Associated with an Aberrant Innate Immunity and with Massive Lymphocyte Apoptosis. PLoS Negl. Trop. Dis., 4.","DOI":"10.1371\/journal.pntd.0000837"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1002\/hep.1840130226","article-title":"Effects of Cytokines on the Liver","volume":"13","author":"Andus","year":"1991","journal-title":"Hepatology"},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1016\/j.jhep.2016.02.004","article-title":"IL-6 Pathway in the Liver: From Physiopathology to Therapy","volume":"64","year":"2016","journal-title":"J. Hepatol."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1097\/AIA.0b013e318034194e","article-title":"Cytokines, Inflammation and Pain","volume":"45","author":"Zhang","year":"2007","journal-title":"Int. Anesth. Clin."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1016\/j.bcmd.2004.09.001","article-title":"Blood Coagulation Factors as Inflammatory Mediators","volume":"34","author":"Schoenmakers","year":"2005","journal-title":"Blood Cells Mol. Dis."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"3399","DOI":"10.1161\/01.ATV.17.12.3399","article-title":"Effect of Human Recombinant Interleukin-6 and Interleukin-8 on Monocyte Procoagulant Activity","volume":"17","author":"Neumann","year":"1997","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_76","first-page":"967","article-title":"Cytokines and Hemostasis","volume":"85","author":"Grignani","year":"2000","journal-title":"Haematologica"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"326","DOI":"10.3109\/10409238.2015.1050550","article-title":"How It All Starts: Initiation of the Clotting Cascade","volume":"50","author":"Smith","year":"2015","journal-title":"Crit. Rev. Biochem. Mol. Biol."},{"key":"ref_78","doi-asserted-by":"crossref","unstructured":"Smither, S.J., O\u2019Brien, L.M., Eastaugh, L., Woolley, T., Lever, M., Fletcher, T., Parmar, K., Hunt, B.J., Watts, S., and Kirkman, E. (2019). Haemostatic Changes in Five Patients Infected with Ebola Virus. Viruses, 11.","DOI":"10.3390\/v11070647"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"20140035","DOI":"10.1098\/rstb.2014.0035","article-title":"The Role of Cellular Adhesion Molecules in Virus Attachment and Entry","volume":"370","author":"Bhella","year":"2015","journal-title":"Philos. Trans. R. Soc. Lond. B Biol. Sci."},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"8426","DOI":"10.1073\/pnas.1019030108","article-title":"T-Cell Immunoglobulin and Mucin Domain 1 (TIM-1) Is a Receptor for Zaire Ebolavirus and Lake Victoria Marburgvirus","volume":"108","author":"Kondratowicz","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"327","DOI":"10.4049\/jimmunol.0901231","article-title":"Mechanisms and Consequences of Ebolavirus-Induced Lymphocyte Apoptosis","volume":"184","author":"Bradfute","year":"2010","journal-title":"J. Immunol."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1038\/ni1519","article-title":"Immunopathology of Highly Virulent Pathogens: Insights from Ebola Virus","volume":"8","author":"Zampieri","year":"2007","journal-title":"Nat. Immunol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1016\/j.virol.2004.11.018","article-title":"A Reconstituted Replication and Transcription System for Ebola Virus Reston and Comparison with Ebola Virus Zaire","volume":"332","author":"Boehmann","year":"2005","journal-title":"Virology"},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1126\/science.1113329","article-title":"Modulation of Hepatitis C Virus RNA Abundance by a Liver-Specific MicroRNA","volume":"309","author":"Jopling","year":"2005","journal-title":"Science"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1016\/j.chom.2014.07.006","article-title":"Hepatitis C Virus Subverts Liver-Specific MiR-122 to Protect the Viral Genome from Exoribonuclease Xrn2","volume":"16","author":"Sedano","year":"2014","journal-title":"Cell Host Microbe"},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"217","DOI":"10.1016\/j.chom.2014.12.014","article-title":"MiR-122 Stimulates Hepatitis C Virus RNA Synthesis by Altering the Balance of Viral RNAs Engaged in Replication versus Translation","volume":"17","author":"Masaki","year":"2015","journal-title":"Cell Host Microbe"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1111\/jcmm.14045","article-title":"Inhibition of MiR-148a-3p Resists Hepatocellular Carcinoma Progress of Hepatitis C Virus Infection through Suppressing C-Jun and MAPK Pathway","volume":"23","author":"Deng","year":"2019","journal-title":"J. Cell Mol. Med."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1226","DOI":"10.1111\/liv.14112","article-title":"Activation of the Oncogenic MiR-21-5p Promotes HCV Replication and Steatosis Induced by the Viral Core 3a Protein","volume":"39","author":"Sobolewski","year":"2019","journal-title":"Liver Int."},{"key":"ref_89","first-page":"S21","article-title":"Course and Outcome of Hepatitis C","volume":"36","author":"Hoofnagle","year":"2002","journal-title":"Hepatology"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"396","DOI":"10.3389\/fmicb.2018.00396","article-title":"Molecular Basis of Encapsidation of Hepatitis C Virus Genome","volume":"9","author":"Shi","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Shabman, R.S., Hoenen, T., Groseth, A., Jabado, O., Binning, J.M., Amarasinghe, G.K., Feldmann, H., and Basler, C.F. (2013). An Upstream Open Reading Frame Modulates Ebola Virus Polymerase Translation and Virus Replication. PLoS Pathog., 9.","DOI":"10.1371\/journal.ppat.1003147"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"46374","DOI":"10.1038\/srep46374","article-title":"Structure of the Ebola Virus Glycoprotein Spike within the Virion Envelope at 11 \u00c5 Resolution","volume":"7","author":"Beniac","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_93","doi-asserted-by":"crossref","unstructured":"Niepmann, M., and Gerresheim, G.K. (2020). Hepatitis C Virus Translation Regulation. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21072328"},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1126\/science.2523562","article-title":"Isolation of a CDNA Clone Derived from a Blood-Borne Non-A, Non-B Viral Hepatitis Genome","volume":"244","author":"Choo","year":"1989","journal-title":"Science"},{"key":"ref_95","doi-asserted-by":"crossref","first-page":"1055","DOI":"10.1099\/vir.0.008300-0","article-title":"Hepatitis C Virus Cell Entry: Role of Lipoproteins and Cellular Receptors","volume":"90","author":"Burlone","year":"2009","journal-title":"J. Gen. Virol."},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1126\/science.1178178","article-title":"Therapeutic Silencing of MicroRNA-122 in Primates with Chronic Hepatitis C Virus Infection","volume":"327","author":"Lanford","year":"2010","journal-title":"Science"},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"2378","DOI":"10.1002\/hep.26302","article-title":"A C-Myc-MicroRNA Functional Feedback Loop Affects Hepatocarcinogenesis","volume":"57","author":"Han","year":"2013","journal-title":"Hepatology"},{"key":"ref_98","doi-asserted-by":"crossref","unstructured":"Drobna, M., Szarzy\u0144ska, B., Jaksik, R., S\u0119dek, \u0141., Kuchmiy, A., Taghon, T., van Vlierberghe, P., Szczepa\u0144ski, T., Witt, M., and Dawidowska, M. (2020). Hsa-MiR-20b-5p and Hsa-MiR-363-3p Affect Expression of PTEN and BIM Tumor Suppressor Genes and Modulate Survival of T-ALL Cells In Vitro. Cells, 9.","DOI":"10.3390\/cells9051137"},{"key":"ref_99","doi-asserted-by":"crossref","unstructured":"Ayoubian, H., Ludwig, N., Fehlmann, T., Menegatti, J., Gr\u00f6ger, L., Anastasiadou, E., Trivedi, P., Keller, A., Meese, E., and Gr\u00e4sser, F.A. (2019). Epstein-Barr Virus Infection of Cell Lines Derived from Diffuse Large B-Cell Lymphomas Alters MicroRNA Loading of the Ago2 Complex. J. Virol., 93.","DOI":"10.1128\/JVI.01297-18"},{"key":"ref_100","doi-asserted-by":"crossref","unstructured":"Zhang, F., Lin, X., Yang, X., Lu, G., Zhang, Q., and Zhang, C. (2019). MicroRNA-132-3p Suppresses Type I IFN Response through Targeting IRF1 to Facilitate H1N1 Influenza A Virus Infection. Biosci. Rep., 39.","DOI":"10.1042\/BSR20192769"},{"key":"ref_101","doi-asserted-by":"crossref","unstructured":"Zheng, H., Xu, L., Liu, Y., Li, C., Zhang, L., Wang, T., Zhao, D., Xu, X., and Zhang, Y. (2018). MicroRNA-221-5p Inhibits Porcine Epidemic Diarrhea Virus Replication by Targeting Genomic Viral RNA and Activating the NF-\u039aB Pathway. Int. J. Mol. Sci., 19.","DOI":"10.3390\/ijms19113381"},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"34034","DOI":"10.1038\/srep34034","article-title":"Up-Regulation of MiR-95-3p in Hepatocellular Carcinoma Promotes Tumorigenesis by Targeting P21 Expression","volume":"6","author":"Ye","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"103922","DOI":"10.1016\/j.meegid.2019.103922","article-title":"Integration Analysis of a MiRNA-MRNA Expression in A549 Cells Infected with a Novel H3N2 Swine Influenza Virus and the 2009 H1N1 Pandemic Influenza Virus","volume":"74","author":"Gao","year":"2019","journal-title":"Infect. Genet. Evol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"8849","DOI":"10.1128\/JVI.00456-10","article-title":"Cellular MicroRNAs Inhibit Replication of the H1N1 Influenza A Virus in Infected Cells","volume":"84","author":"Song","year":"2010","journal-title":"J. Virol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1016\/j.celrep.2017.09.030","article-title":"Host MicroRNAs-221 and -222 Inhibit HIV-1 Entry in Macrophages by Targeting the CD4 Viral Receptor","volume":"21","author":"Lodge","year":"2017","journal-title":"Cell Rep."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"1159","DOI":"10.1128\/JVI.02386-12","article-title":"Increasing Expression of MicroRNA 181 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication and Has Implications for Controlling Virus Infection","volume":"87","author":"Guo","year":"2013","journal-title":"J. Virol."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"13830","DOI":"10.1073\/pnas.1008861107","article-title":"Combined Agonist\u2013Antagonist Genome-Wide Functional Screening Identifies Broadly Active Antiviral MicroRNAs","volume":"107","author":"Santhakumar","year":"2010","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_108","doi-asserted-by":"crossref","first-page":"1241","DOI":"10.1038\/nm1639","article-title":"Cellular MicroRNAs Contribute to HIV-1 Latency in Resting Primary CD4 + T Lymphocytes","volume":"13","author":"Huang","year":"2007","journal-title":"Nat. Med."},{"key":"ref_109","doi-asserted-by":"crossref","first-page":"2795","DOI":"10.2147\/CMAR.S196655","article-title":"High MiR-454-3p Expression Predicts Poor Prognosis in Hepatocellular Carcinoma","volume":"11","author":"Li","year":"2019","journal-title":"Cancer Manag. Res."},{"key":"ref_110","doi-asserted-by":"crossref","unstructured":"Versteeg, K., Menicucci, A.R., Woolsey, C., Mire, C.E., Geisbert, J.B., Cross, R.W., Agans, K.N., Jeske, D., Messaoudi, I., and Geisbert, T.W. (2017). Infection with the Makona Variant Results in a Delayed and Distinct Host Immune Response Compared to Previous Ebola Virus Variants. Sci. Rep., 7.","DOI":"10.1038\/s41598-017-09963-y"},{"key":"ref_111","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1189\/jlb.1211593","article-title":"MiRNA-125b Regulates TNF-\u03b1 Production in CD14+ Neonatal Monocytes via Post-Transcriptional Regulation","volume":"92","author":"Huang","year":"2012","journal-title":"J. Leukoc. Biol."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1016\/j.cell.2006.07.031","article-title":"A Pattern-Based Method for the Identification of MicroRNA Binding Sites and Their Corresponding Heteroduplexes","volume":"126","author":"Miranda","year":"2006","journal-title":"Cell"},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1097\/CCO.0b013e32835b6386","article-title":"Interactions between the Tumor Suppressor P53 and Immune Responses","volume":"25","author":"Menendez","year":"2013","journal-title":"Curr. Opin. Oncol."},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13045-019-0754-1","article-title":"Targeting MTOR for Cancer Therapy","volume":"12","author":"Hua","year":"2019","journal-title":"J. Hematol. Oncol."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1016\/j.bbamcr.2007.03.010","article-title":"P38 MAP-Kinases Pathway Regulation, Function and Role in Human Diseases","volume":"1773","author":"Cuenda","year":"2007","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"W244","DOI":"10.1093\/nar\/gkaa467","article-title":"MiRNet 2.0: Network-Based Visual Analytics for MiRNA Functional Analysis and Systems Biology","volume":"48","author":"Chang","year":"2020","journal-title":"Nucleic Acids Res."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"7083","DOI":"10.1016\/j.vaccine.2018.09.016","article-title":"Dynamic Changes of Circulating MiRNAs Induced by the Ebola Virus Vaccine VSV-EBOV","volume":"36","author":"Fischer","year":"2018","journal-title":"Vaccine"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"e23590","DOI":"10.1002\/jcla.23590","article-title":"Differential MicroRNA Expression in the Peripheral Blood from Human Patients with COVID-19","volume":"34","author":"Li","year":"2020","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_119","first-page":"157","article-title":"WHO Declares COVID-19 a Pandemic","volume":"91","author":"Cucinotta","year":"2020","journal-title":"Acta Biomed."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1967","DOI":"10.1016\/S0140-6736(20)31379-9","article-title":"COVID-19, Nuclear War, and Global Warming: Lessons for Our Vulnerable World","volume":"395","author":"Muller","year":"2020","journal-title":"Lancet"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/B978-0-12-418687-3.00018-5","article-title":"Transient Mammalian Cell Transfection with Polyethylenimine (PEI)","volume":"529","author":"Longo","year":"2013","journal-title":"Methods Enzym."},{"key":"ref_122","doi-asserted-by":"crossref","unstructured":"Benmoussa, A., Diallo, I., Salem, M., Michel, S., Gilbert, C., S\u00e9vigny, J., and Provost, P. (2019). Concentrates of Two Subsets of Extracellular Vesicles from Cow\u2019s Milk Modulate Symptoms and Inflammation in Experimental Colitis. Sci. Rep., 9.","DOI":"10.1038\/s41598-019-51092-1"},{"key":"ref_123","doi-asserted-by":"crossref","unstructured":"Ye, J., Coulouris, G., Zaretskaya, I., Cutcutache, I., Rozen, S., and Madden, T.L. (2012). Primer-BLAST: A Tool to Design Target-Specific Primers for Polymerase Chain Reaction. BMC Bioinform., 13.","DOI":"10.1186\/1471-2105-13-134"},{"key":"ref_124","doi-asserted-by":"crossref","first-page":"749","DOI":"10.3858\/emm.2010.42.11.076","article-title":"Suitable Reference Genes for Relative Quantification of MiRNA Expression in Prostate Cancer","volume":"42","author":"Schaefer","year":"2010","journal-title":"Exp. Mol. Med."},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2(-Delta Delta C(T)) Method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_126","doi-asserted-by":"crossref","unstructured":"Bullard, J.H., Purdom, E., Hansen, K.D., and Dudoit, S. (2010). Evaluation of Statistical Methods for Normalization and Differential Expression in MRNA-Seq Experiments. BMC Bioinform., 11.","DOI":"10.1186\/1471-2105-11-94"},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1038\/nbt1394","article-title":"Discovering MicroRNAs from Deep Sequencing Data Using MiRDeep","volume":"26","author":"Chen","year":"2008","journal-title":"Nat. Biotechnol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/7\/3792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T14:11:26Z","timestamp":1760364686000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/7\/3792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,6]]},"references-count":127,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2021,4]]}},"alternative-id":["ijms22073792"],"URL":"https:\/\/doi.org\/10.3390\/ijms22073792","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,6]]}}}