{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,20]],"date-time":"2026-06-20T05:50:24Z","timestamp":1781934624772,"version":"3.54.5"},"reference-count":34,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2017\/25\/B\/NZ3\/00122"],"award-info":[{"award-number":["2017\/25\/B\/NZ3\/00122"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2020\/37\/B\/NZ5\/00100"],"award-info":[{"award-number":["2020\/37\/B\/NZ5\/00100"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004561","name":"Ministry of Education and Science of the Republic of Kazakhstan","doi-asserted-by":"publisher","award":["SHIP-2.3\/CS-02"],"award-info":[{"award-number":["SHIP-2.3\/CS-02"]}],"id":[{"id":"10.13039\/501100004561","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Although malignant ascites (MAs) are known to contribute to various aspects of ovarian cancer progression, knowledge regarding their role in the adhesion of cancer cells to normal peritoneal cells is incomplete. Here, we compared the effect of MAs and benign ascites (BAs) on the adhesion of A2780 and OVCAR-3 cancer cells to omentum-derived peritoneal mesothelial cells (PMCs) and peritoneal fibroblasts (PFBs). The results showed that MAs stimulated the adhesion of A2780 and OVCAR-3 cells to PMCs and PFBs more efficiently than did BAs, and the strongest binding occurred when both cancer and normal cells were exposed to the fluid. Intervention studies showed that MAs-driven adhesion of A2780 cells to PMCs\/PFBs depends on the presence of TGF-\u03b21 and HGF, whereas binding of OVCAR-3 cells was mediated by TGF-\u03b21, GRO-1, and IGF-1. Moreover, MAs upregulated \u03b15\u03b21 integrin expression on PFBs but not on PMCs or cancer cells, vimentin expression in all cells tested, and ICAM-1 only in cancer cells. When integrin-linked kinase was neutralized in PMCs or PFBs, cancer cell adhesion to PMCs and PFBs decreased. Collectively, our report shows that MAs may contribute to the early stages of ovarian cancer metastasis by modulating the proadhesive interplay between normal and cancer cells.<\/jats:p>","DOI":"10.3390\/ijms22084222","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T11:21:38Z","timestamp":1618831298000},"page":"4222","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Malignant Ascites Promote Adhesion of Ovarian Cancer Cells to Peritoneal Mesothelium and Fibroblasts"],"prefix":"10.3390","volume":"22","author":[{"given":"Pawe\u0142","family":"Uruski","sequence":"first","affiliation":[{"name":"Department of Hypertensiology, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8267-5428","authenticated-orcid":false,"given":"Justyna","family":"Miku\u0142a-Pietrasik","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martyna","family":"Paku\u0142a","sequence":"additional","affiliation":[{"name":"Department of Hypertensiology, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1856-2680","authenticated-orcid":false,"given":"Sylwia","family":"Budkiewicz","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7562-0904","authenticated-orcid":false,"given":"Marcin","family":"Drzewiecki","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7629-8737","authenticated-orcid":false,"given":"Andrey N.","family":"Gaiday","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, West Kazakhstan Marat Ospanov Medical University, 50B 12th Microdistrict Apt.21, Aktobe 030008, Kazakhstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Wierzowiecka","sequence":"additional","affiliation":[{"name":"Department of Hypertensiology, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eryk","family":"Naumowicz","sequence":"additional","affiliation":[{"name":"General Surgery Ward, Medical Centre HCP, 28 Czerwca 1956 r. 223\/229 Str., 61-485 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5121-5026","authenticated-orcid":false,"given":"Rafa\u0142","family":"Moszy\u0144ski","sequence":"additional","affiliation":[{"name":"Division of Gynecological Surgery, Poznan University of Medical Sciences, Polna 33 Str., 60-535 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Tykarski","sequence":"additional","affiliation":[{"name":"Department of Hypertensiology, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6066-5747","authenticated-orcid":false,"given":"Krzysztof","family":"Ksi\u0105\u017cek","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology of Ageing and Civilization Diseases, Poznan University of Medical Sciences, D\u0142uga 1\/2 Str., 61-848 Poznan, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.3322\/caac.21166","article-title":"Cancer statistics, 2013","volume":"63","author":"Siegel","year":"2013","journal-title":"CA Cancer J. 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