{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:14:53Z","timestamp":1792678493476,"version":"4.5.0"},"reference-count":48,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T00:00:00Z","timestamp":1618790400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Czech Science Foundation","award":["18-02993S, 21-10845S"],"award-info":[{"award-number":["18-02993S, 21-10845S"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Glucocorticoids (GCs) are hormones that are released in response to stressors and exhibit many activities, including immunomodulatory and anti-inflammatory activities. They are primarily synthesized in the adrenal gland but are also produced in peripheral tissues via regeneration of adrenal 11-oxo metabolites or by de novo synthesis from cholesterol. The present study investigated the influence of the microbiota on de novo steroidogenesis and regeneration of corticosterone in the intestine of germ-free (GF) and specific pathogen-free mice challenged with a physical stressor (anti-CD3 antibody i.p. injection). In the small intestine, acute immune stress resulted in increased mRNA levels of the proinflammatory cytokines IL1\u03b2, IL6 and Tnf\u03b1 and genes involved in de novo steroidogenesis (Stard3 and Cyp11a1), as well as in regeneration of active GCs from their 11-oxo metabolites (Hsd11b1). GF mice showed a generally reduced transcriptional response to immune stress, which was accompanied by decreased intestinal corticosterone production and reduced expression of the GC-sensitive marker Fkbp5. In contrast, the interaction between stress and the microbiota was not detected at the level of plasma corticosterone or the transcriptional response of adrenal steroidogenic enzymes. The results indicate a differential immune stress-induced intestinal response to proinflammatory stimuli and local corticosterone production driven by the gut microbiota.<\/jats:p>","DOI":"10.3390\/ijms22084229","type":"journal-article","created":{"date-parts":[[2021,4,19]],"date-time":"2021-04-19T11:21:38Z","timestamp":1618831298000},"page":"4229","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["The Gut Microbiota Affects Corticosterone Production in the Murine Small Intestine"],"prefix":"10.3390","volume":"22","author":[{"given":"Peter","family":"Ergang","sequence":"first","affiliation":[{"name":"Institute of Physiology, Czech Academy of Sciences, CZ-142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karla","family":"Vagnerov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Czech Academy of Sciences, CZ-142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petra","family":"Hermanov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences, CZ-549 22 Nov\u00fd Hr\u00e1dek, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Vodi\u010dka","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Czech Academy of Sciences, CZ-142 20 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"J\u00e1gr","sequence":"additional","affiliation":[{"name":"Crop Research Institute, CZ-161 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dagmar","family":"\u0160r\u016ftkov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences, CZ-549 22 Nov\u00fd Hr\u00e1dek, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e1clav","family":"Dvo\u0159\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Crop Research Institute, CZ-161 06 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom\u00e1\u0161","family":"Hudcovic","sequence":"additional","affiliation":[{"name":"Institute of Microbiology, Czech Academy of Sciences, CZ-549 22 Nov\u00fd Hr\u00e1dek, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2335-6715","authenticated-orcid":false,"given":"Ji\u0159\u00ed","family":"P\u00e1cha","sequence":"additional","affiliation":[{"name":"Institute of Physiology, Czech Academy of Sciences, CZ-142 20 Prague, Czech Republic"},{"name":"Department of Physiology, Faculty of Science, Charles University, CZ-128 00 Prague, Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1152\/physrev.00018.2018","article-title":"The Microbiota-Gut-Brain Axis","volume":"99","author":"Cryan","year":"2019","journal-title":"Physiol. 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