{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T01:58:28Z","timestamp":1760234308063,"version":"build-2065373602"},"reference-count":52,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007397","name":"Charles University","doi-asserted-by":"publisher","award":["PROGRES Q39","Research Centre program UNCE\/MED\/006 University Center of Clinical and Experimental Liver Surgery"],"award-info":[{"award-number":["PROGRES Q39","Research Centre program UNCE\/MED\/006 University Center of Clinical and Experimental Liver Surgery"]}],"id":[{"id":"10.13039\/100007397","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007280 Application of Modern Technologies in Medicine and Industry"],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/17_048\/0007280 Application of Modern Technologies in Medicine and Industry"]}]},{"name":"Ministry of Education, Youth and Sports of the Czech Republic, financed from The European Regional Development Fund","award":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000787 \u201eFighting INfectious Diseases\""],"award-info":[{"award-number":["CZ.02.1.01\/0.0\/0.0\/16_019\/0000787 \u201eFighting INfectious Diseases\""]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>In liver surgery, biliary obstruction can lead to secondary biliary cirrhosis, a life-threatening disease with liver transplantation as the only curative treatment option. Mesenchymal stromal cells (MSC) have been shown to improve liver function in both acute and chronic liver disease models. This study evaluated the effect of allogenic MSC transplantation in a large animal model of repeated biliary obstruction followed by partial hepatectomy. MSC transplantation supported the growth of regenerated liver tissue after 14 days (MSC group, n = 10: from 1087 \u00b1 108 (0 h) to 1243 \u00b1 92 mL (14 days); control group, n = 11: from 1080 \u00b1 95 (0 h) to 1100 \u00b1 105 mL (14 days), p = 0.016), with a lower volume fraction of hepatocytes in regenerated liver tissue compared to resected liver tissue (59.5 \u00b1 10.2% vs. 70.2 \u00b1 5.6%, p &lt; 0.05). Volume fraction of connective tissue, blood vessels and bile vessels in regenerated liver tissue, serum levels of liver enzymes (AST, ALT, ALP and GGT) and liver metabolites (albumin, bilirubin, urea and creatinine), as well as plasma levels of IL-6, IL-8, TNF-\u03b1 and TGF-\u03b2, were not affected by MSC transplantation. In our novel, large animal (pig) model of repeated biliary obstruction followed by partial hepatectomy, MSC transplantation promoted growth of liver tissue without any effect on liver function. This study underscores the importance of translating results between small and large animal models as well as the careful translation of results from animal model into human medicine.<\/jats:p>","DOI":"10.3390\/ijms22094304","type":"journal-article","created":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T12:47:07Z","timestamp":1619009227000},"page":"4304","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mesenchymal Stromal Cell Therapy in Novel Porcine Model of Diffuse Liver Damage Induced by Repeated Biliary Obstruction"],"prefix":"10.3390","volume":"22","author":[{"given":"Lucie","family":"Vi\u0161tejnov\u00e1","sequence":"first","affiliation":[{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, 323 00 Pilsen, Czech Republic"},{"name":"Department of Histology and Embryology, Faculty of Medicine in Pilsen, Charles University, Karlovarska 48, 301 00 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V\u00e1clav","family":"Li\u0161ka","sequence":"additional","affiliation":[{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, 323 00 Pilsen, Czech Republic"},{"name":"Department of Surgery, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arvind","family":"Kumar","sequence":"additional","affiliation":[{"name":"Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Alej Svobody 76, 323 00 Pilsen, Czech Republic"},{"name":"Department of Surgery, Medical School and Teaching Hospital in Pilsen, Charles University, Alej Svobody 80, 304 60 Pilsen, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"K\u0159e\u010dkov\u00e1","sequence":"additional","affiliation":[{"name":"Biomedical 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