{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T12:02:22Z","timestamp":1790424142459,"version":"4.1.0"},"reference-count":75,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T00:00:00Z","timestamp":1618963200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["1"],"award-info":[{"award-number":["1"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007586","name":"Aix-Marseille Universit\u00e9","doi-asserted-by":"publisher","award":["2"],"award-info":[{"award-number":["2"]}],"id":[{"id":"10.13039\/100007586","id-type":"DOI","asserted-by":"publisher"}]},{"name":"AFM-T\u00e9l\u00e9thon","award":["(Strategic pole MNH Decrypt)"],"award-info":[{"award-number":["(Strategic pole MNH Decrypt)"]}]},{"name":"Promex Stiftung F\u00fcr Die Forschung","award":["3"],"award-info":[{"award-number":["3"]}]},{"DOI":"10.13039\/100001819","name":"Rettsyndrome.org","doi-asserted-by":"publisher","award":["4"],"award-info":[{"award-number":["4"]}],"id":[{"id":"10.13039\/100001819","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Association Fran\u00e7aise du Syndrome de Rett (AFSR)","award":["5"],"award-info":[{"award-number":["5"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Mutations in the X-linked MECP2 gene are responsible for Rett syndrome (RTT), a severe neurological disorder. MECP2 is a transcriptional modulator that finely regulates the expression of many genes, specifically in the central nervous system. Several studies have functionally linked the loss of MECP2 in astrocytes to the appearance and progression of the RTT phenotype in a non-cell autonomous manner and mechanisms are still unknown. Here, we used primary astroglial cells from Mecp2-deficient (KO) pups to identify deregulated secreted proteins. Using a differential quantitative proteomic analysis, twenty-nine proteins have been identified and four were confirmed by Western blotting with new samples as significantly deregulated. To further verify the functional relevance of these proteins in RTT, we tested their effects on the dendritic morphology of primary cortical neurons from Mecp2 KO mice that are known to display shorter dendritic processes. Using Sholl analysis, we found that incubation with Lcn2 or Lgals3 for 48 h was able to significantly increase the dendritic arborization of Mecp2 KO neurons. To our knowledge, this study, through secretomic analysis, is the first to identify astroglial secreted proteins involved in the neuronal RTT phenotype in vitro, which could open new therapeutic avenues for the treatment of Rett syndrome.<\/jats:p>","DOI":"10.3390\/ijms22094316","type":"journal-article","created":{"date-parts":[[2021,4,21]],"date-time":"2021-04-21T12:47:07Z","timestamp":1619009227000},"page":"4316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Analysis of Astroglial Secretomic Profile in the Mecp2-Deficient Male Mouse Model of Rett Syndrome"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9314-6575","authenticated-orcid":false,"given":"Yann","family":"Ehinger","sequence":"first","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valerie","family":"Matagne","sequence":"additional","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Cunin","sequence":"additional","affiliation":[{"name":"Inserm U1055, LBFA and BEeSy, Prom\u00e9th\u00e9e Proteomics Platform, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"},{"name":"Prom\u00e9th\u00e9e Proteomics Platform, Institut de Biologie et de Pathologie, CHU Grenoble Alpes, 38000 Grenoble, France"},{"name":"University Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC, PROMETHEE Proteomic Platform, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6983-6461","authenticated-orcid":false,"given":"Emilie","family":"Borloz","sequence":"additional","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5503-9488","authenticated-orcid":false,"given":"Michel","family":"Seve","sequence":"additional","affiliation":[{"name":"Inserm U1055, LBFA and BEeSy, Prom\u00e9th\u00e9e Proteomics Platform, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"},{"name":"Prom\u00e9th\u00e9e Proteomics Platform, Institut de Biologie et de Pathologie, CHU Grenoble Alpes, 38000 Grenoble, France"},{"name":"University Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC, PROMETHEE Proteomic Platform, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6511-0612","authenticated-orcid":false,"given":"Sandrine","family":"Bourgoin-Voillard","sequence":"additional","affiliation":[{"name":"Inserm U1055, LBFA and BEeSy, Prom\u00e9th\u00e9e Proteomics Platform, Universit\u00e9 Grenoble Alpes, 38000 Grenoble, France"},{"name":"Prom\u00e9th\u00e9e Proteomics Platform, Institut de Biologie et de Pathologie, CHU Grenoble Alpes, 38000 Grenoble, France"},{"name":"University Grenoble Alpes, CNRS, Grenoble INP, CHU Grenoble Alpes, TIMC, PROMETHEE Proteomic Platform, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Borges-Correia","sequence":"additional","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6657-5008","authenticated-orcid":false,"given":"Laurent","family":"Villard","sequence":"additional","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Roux","sequence":"additional","affiliation":[{"name":"Aix Marseille University, Inserm, MMG, Marseille Medical Genetics, 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1038\/13810","article-title":"Rett syndrome is caused by mutations in X-linked MECP2, encoding methyl-CpG-binding protein 2","volume":"23","author":"Amir","year":"1999","journal-title":"Nat. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1002\/ana.410140412","article-title":"A progressive syndrome of autism, dementia, ataxia, and loss of purposeful hand use in girls: Rett\u2019s syndrome: Report of 35 cases","volume":"14","author":"Hagberg","year":"1983","journal-title":"Ann. Neurol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"368","DOI":"10.1038\/s41583-018-0006-3","article-title":"Rett syndrome: Insights into genetic, molecular and circuit mechanisms","volume":"19","author":"Ip","year":"2018","journal-title":"Nat. Rev. Neurosci."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1016\/j.mcn.2004.07.006","article-title":"MECP2 is progressively expressed in post-migratory neurons and is involved in neuronal maturation rather than cell fate decisions","volume":"27","author":"Kishi","year":"2004","journal-title":"Mol. Cell. Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1016\/j.neuroscience.2003.11.025","article-title":"Developmental expression of methyl-CpG binding protein 2 is dy-namically regulated in the rodent brain","volume":"123","author":"Mullaney","year":"2004","journal-title":"Neuroscience"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"430","DOI":"10.1016\/j.neuron.2008.10.013","article-title":"The mystery and magic of glia: A perspective on their roles in health and disease","volume":"60","author":"Barres","year":"2008","journal-title":"Neuron"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.neuroscience.2018.11.010","article-title":"Diversity and specificity of astrocyte-neuron communication","volume":"396","author":"Durkee","year":"2019","journal-title":"Neuroscience"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"154","DOI":"10.1038\/s41593-018-0325-8","article-title":"Astrocyte function from information processing to cognition and cognitive impairment","volume":"22","author":"Santello","year":"2019","journal-title":"Nat. Neurosci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1093\/hmg\/11.2.115","article-title":"Insight into Rett syndrome: MeCP2 levels dis-play tissue- and cell-specific differences and correlate with neuronal maturation","volume":"11","author":"Shahbazian","year":"2002","journal-title":"Hum. Mol. Genet."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"311","DOI":"10.1038\/nn.2275","article-title":"Non-cell autonomous influence of MeCP2-deficient glia on neuronal dendritic morphology","volume":"12","author":"Ballas","year":"2009","journal-title":"Nat. Neurosci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"5051","DOI":"10.1523\/JNEUROSCI.0324-09.2009","article-title":"Rett syndrome astrocytes are abnormal and spread MeCP2 deficiency through gap junctions","volume":"29","author":"Maezawa","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1038\/nature10214","article-title":"A role for glia in the progression of Rett\u2019s syndrome","volume":"475","author":"Lioy","year":"2011","journal-title":"Nature"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1007\/s12031-015-0597-3","article-title":"Involvement of MeCP2 in regulation of myelin-related gene ex-pression in cultured rat oligodendrocytes","volume":"57","author":"Sharma","year":"2015","journal-title":"J. Mol. Neurosci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1186\/1744-8069-8-14","article-title":"The expression of spinal methyl-CpG-binding protein 2, DNA methyltransferases and histone deacetylases is modulated in persistent pain states","volume":"8","author":"Tochiki","year":"2012","journal-title":"Mol. Pain."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"670","DOI":"10.1152\/japplphysiol.00411.2015","article-title":"Conditional depletion of methyl-CpG-binding protein 2 in astrocytes depresses the hypercapnic ventilatory response in mice","volume":"119","author":"Garg","year":"2015","journal-title":"J. Appl. Physiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3159","DOI":"10.1113\/JP270369","article-title":"Impaired CO2 sensitivity of astrocytes in a mouse model of Rett syndrome","volume":"593","author":"Turovsky","year":"2015","journal-title":"J. Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"353","DOI":"10.1007\/s12017-015-8363-9","article-title":"Astrocyte transcriptome from the Mecp2(308)-truncated mouse model of rett syndrome","volume":"17","author":"Delepine","year":"2015","journal-title":"Neuromol. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e31629","DOI":"10.7554\/eLife.31629","article-title":"Astrocytic modulation of excitatory synaptic signaling in a mouse model of Rett syndrome","volume":"7","author":"Rakela","year":"2018","journal-title":"Elife"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4579","DOI":"10.1021\/pr900383g","article-title":"The cell line secre-tome, a suitable tool for investigating proteins released in vivo by tumors: Application to the study of p53-modulated proteins secreted in lung cancer cells","volume":"8","author":"Chenau","year":"2009","journal-title":"J. Proteome. Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1080\/14789450.2020.1745069","article-title":"Proteomic examination of the neuroglial secretome: Lessons for the clinic","volume":"17","author":"Kim","year":"2020","journal-title":"Expert Rev. Proteom."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Song, P., Kwon, Y., Joo, J.Y., Kim, D.G., and Yoon, J.H. (2019). Secretomics to discover regulators in diseases. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20163893"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2968","DOI":"10.1093\/hmg\/ddu008","article-title":"Mutant astrocytes differentiated from Rett syndrome pa-tients-specific iPSCs have adverse effects on wild-type neurons","volume":"23","author":"Williams","year":"2014","journal-title":"Hum. Mol. Genet."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1681","DOI":"10.1007\/s00726-019-02796-1","article-title":"Modulation of muscle protein synthesis by amino acids: What consequences for the secretome? A preliminary in vitro study","volume":"51","author":"Goron","year":"2019","journal-title":"Amino Acids"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1038\/nbt.2839","article-title":"ProteomeXchange provides globally coordinated proteomics data submission and dissemination","volume":"32","author":"Vizcaino","year":"2014","journal-title":"Nat. Biotechnol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"826","DOI":"10.1126\/science.1089071","article-title":"Postnatal neurodevelopmental disorders: Meeting at the synapse?","volume":"302","author":"Zoghbi","year":"2003","journal-title":"Science"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"747","DOI":"10.1177\/08830738050200082401","article-title":"Neuropathology of Rett syndrome","volume":"20","author":"Armstrong","year":"2005","journal-title":"J. Child. Neurol."},{"key":"ref_27","first-page":"603","article-title":"Differential neurite growth on astrocyte substrates: Interspecies facilitation in green fluorescent protein-transfected rat and human neurons","volume":"95","author":"Spencer","year":"2000","journal-title":"Neuroscience"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"122","DOI":"10.3389\/fncel.2013.00122","article-title":"Lipocalin-2 is involved in emotional behaviors and cognitive function","volume":"7","author":"Ferreira","year":"2013","journal-title":"Front. Cell Neurosci."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1038\/mp.2017.95","article-title":"Lipocalin-2 regulates adult neurogenesis and contextual discriminative behaviours","volume":"23","author":"Ferreira","year":"2018","journal-title":"Mol. Psychiatry"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s10735-017-9712-9","article-title":"Neuroanatomical distribution of galectin-3 in the adult rat brain","volume":"48","author":"Yoo","year":"2017","journal-title":"J. Mol. Histol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1002\/glia.23730","article-title":"Galectin-3 modulates postnatal subventricular zone gliogenesis","volume":"68","author":"Nicholson","year":"2020","journal-title":"Glia"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1002\/(SICI)1097-4695(199710)33:4<395::AID-NEU5>3.0.CO;2-5","article-title":"Effects of the neurotrophins nerve growth factor, neurotrophin-3, and brain-derived neurotrophic factor (BDNF) on neurite growth from adult sensory neurons in compartmented cultures","volume":"33","author":"Kimpinski","year":"1997","journal-title":"J. Neurobiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"239","DOI":"10.15252\/embj.201592705","article-title":"Astrocytes as secretory cells of the central nervous system: Idiosyncrasies of vesicular secretion","volume":"35","author":"Verkhratsky","year":"2016","journal-title":"EMBO J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"500","DOI":"10.1038\/s41593-020-0602-1","article-title":"Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transcriptomic map","volume":"23","author":"Bayraktar","year":"2020","journal-title":"Nat. Neurosci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"3220","DOI":"10.1016\/j.celrep.2017.11.058","article-title":"Diverging mRNA and protein networks in activated microglia reveal srsf3 suppresses translation of highly upregulated innate immune transcripts","volume":"21","author":"Boutej","year":"2017","journal-title":"Cell Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"917","DOI":"10.1038\/nature03104","article-title":"Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron","volume":"432","author":"Flo","year":"2004","journal-title":"Nature"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1615\/CritRevImmunol.2015012127","article-title":"Lipocalin-2 in the inflammatory activation of brain astrocytes","volume":"35","author":"Lee","year":"2015","journal-title":"Crit. Rev. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3231","DOI":"10.4049\/jimmunol.179.5.3231","article-title":"A dual role of lipocalin 2 in the apoptosis and deramification of activated microglia","volume":"179","author":"Lee","year":"2007","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"234","DOI":"10.1523\/JNEUROSCI.5273-08.2009","article-title":"Lipocalin-2 is an autocrine mediator of re-active astrocytosis","volume":"29","author":"Lee","year":"2009","journal-title":"J. Neurosci."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1626","DOI":"10.1016\/j.celrep.2015.02.012","article-title":"Microglia-secreted galectin-3 acts as a toll-like receptor 4 ligand and contributes to microglial activation","volume":"10","author":"Burguillos","year":"2015","journal-title":"Cell Rep."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"10383","DOI":"10.1523\/JNEUROSCI.1498-12.2012","article-title":"Galectin-3 is required for resident microglia activation and proliferation in response to ischemic injury","volume":"32","author":"Swarup","year":"2012","journal-title":"J. Neurosci."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1038\/nature10907","article-title":"Wild-type microglia arrest pa-thology in a mouse model of Rett syndrome","volume":"484","author":"Derecki","year":"2012","journal-title":"Nature"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"E1","DOI":"10.1038\/nature14444","article-title":"Wild-type microglia do not reverse pathology in mouse models of Rett syndrome","volume":"521","author":"Wang","year":"2015","journal-title":"Nature"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"5367","DOI":"10.1523\/JNEUROSCI.3474-17.2018","article-title":"Epigenetic promoter DNA methylation of miR-124 promotes HIV-1 tat-mediated microglial activation via MECP2-STAT3 axis","volume":"38","author":"Periyasamy","year":"2018","journal-title":"J. Neurosci."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1091\/mbc.e11-09-0784","article-title":"Re-duced expression of MECP2 affects cell commitment and maintenance in neurons by triggering senescence: New perspective for Rett syndrome","volume":"23","author":"Squillaro","year":"2012","journal-title":"Mol. Biol. Cell."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"616","DOI":"10.1016\/j.bbagen.2005.12.020","article-title":"Galectin-3: An open-ended story","volume":"1760","author":"Dumic","year":"2006","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.neubiorev.2014.12.006","article-title":"Diverse functional roles of lipocalin-2 in the central nervous system","volume":"49","author":"Jha","year":"2015","journal-title":"Neurosci. Biobehav. Rev."},{"key":"ref_48","first-page":"305","article-title":"Galectin-3 as a multifunctional protein","volume":"9","author":"Krzeslak","year":"2004","journal-title":"Cell Mol. Biol. Lett."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"97","DOI":"10.3389\/fnana.2014.00097","article-title":"Dendritic spine dysgenesis in Rett syndrome","volume":"8","author":"Xu","year":"2014","journal-title":"Front. Neuroanat."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1145","DOI":"10.1002\/glia.22342","article-title":"Lipocalin 2 is a novel immune mediator of experimental autoimmune encephalomyelitis pathogenesis and is modulated in mul-tiple sclerosis","volume":"60","author":"Berard","year":"2012","journal-title":"Glia"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"4069","DOI":"10.1073\/pnas.1218497110","article-title":"Reactive as-trocytes secrete lcn2 to promote neuron death","volume":"110","author":"Bi","year":"2013","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/1742-2094-8-124","article-title":"Lipocalin 2 in the central nervous sys-tem host response to systemic lipopolysaccharide administration","volume":"8","author":"Ip","year":"2011","journal-title":"J Neuroinflammation"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"5204","DOI":"10.4049\/jimmunol.1301637","article-title":"Phenotypic polarization of activated astrocytes: The critical role of lipocalin-2 in the classical inflammatory activation of astrocytes","volume":"191","author":"Jang","year":"2013","journal-title":"J. Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1176","DOI":"10.1096\/fj.12-222257","article-title":"Secreted protein lipocalin-2 promotes microglial M1 polarization","volume":"27","author":"Jang","year":"2013","journal-title":"FASEB J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1186\/2040-2392-4-3","article-title":"MeCP2 modulates gene expression pathways in astrocytes","volume":"4","author":"Yasui","year":"2013","journal-title":"Mol. Autism"},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2431","DOI":"10.1093\/hmg\/ddp181","article-title":"Mouse models of MeCP2 disorders share gene expression changes in the cerebellum and hypothalamus","volume":"18","author":"Chahrour","year":"2009","journal-title":"Hum. Mol. Genet."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"847","DOI":"10.1006\/nbdi.2001.0428","article-title":"Gene expression profiling in postmortem Rett Syndrome brain: Differential gene expression and patient classification","volume":"8","author":"Colantuoni","year":"2001","journal-title":"Neurobiol. Dis."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"239","DOI":"10.1093\/brain\/awy323","article-title":"Towards a better diagnosis and treatment of Rett syndrome: A model synaptic disorder","volume":"142","author":"Banerjee","year":"2019","journal-title":"Brain"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.brainres.2012.12.008","article-title":"Galectin-3 enhances angiogenic and migratory po-tential of microglial cells via modulation of integrin linked kinase signaling","volume":"1496","author":"Wesley","year":"2013","journal-title":"Brain Res."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1016\/j.freeradbiomed.2020.02.023","article-title":"Compromised immune\/inflammatory responses in Rett syndrome","volume":"152","author":"Pecorelli","year":"2020","journal-title":"Free Radic. Biol. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"217","DOI":"10.3389\/fnmol.2017.00217","article-title":"Galectin-3 negatively regulates hippo-campus-dependent memory formation through inhibition of integrin signaling and galectin-3 phosphorylation","volume":"10","author":"Chen","year":"2017","journal-title":"Front. Mol. Neurosci."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1746","DOI":"10.1038\/cdd.2011.40","article-title":"Galectin-3 drives oligodendrocyte differentiation to control myelin integrity and function","volume":"18","author":"Pasquini","year":"2011","journal-title":"Cell Death Differ."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"112862","DOI":"10.1016\/j.physbeh.2020.112862","article-title":"Impaired emotional response to stress in mice lacking galectin-1 or galectin-3","volume":"220","author":"Sartim","year":"2020","journal-title":"Physiol. Behav."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.neuropharm.2013.03.024","article-title":"BDNF deregulation in Rett syndrome","volume":"76","author":"Li","year":"2014","journal-title":"Neuropharmacology"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"e10889","DOI":"10.15252\/emmm.201910889","article-title":"Huntingtin phosphorylation governs BDNF homeostasis and improves the phenotype of Mecp2 knockout mice","volume":"12","author":"Ehinger","year":"2020","journal-title":"EMBO Mol. Med."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2085","DOI":"10.1161\/STROKEAHA.114.005733","article-title":"Neuronal production of lipocalin-2 as a help-me signal for glial activation","volume":"45","author":"Xing","year":"2014","journal-title":"Stroke"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1016\/j.bbi.2019.01.019","article-title":"The role of galectin-3 in modulation of anxiety state level in mice","volume":"78","author":"Stajic","year":"2019","journal-title":"Brain Behav. Immun."},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1016\/j.jprot.2019.01.009","article-title":"A large scale proteome analysis of the gefitinib primary resistance overcome by KDAC inhibition in KRAS mutated adenocarcinoma cells overexpressing amphiregulin","volume":"195","author":"Lehmann","year":"2019","journal-title":"J. Proteomics"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"5140360","DOI":"10.1155\/2017\/5140360","article-title":"Tubulin beta-3 chain as a new candidate protein biomarker of human skin aging: A preliminary study","volume":"2017","author":"Lehmann","year":"2017","journal-title":"Oxid. Med. Cell Longev."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"2758","DOI":"10.1021\/pr1012784","article-title":"General statistical modeling of data from protein rel-ative expression isobaric tags","volume":"10","author":"Breitwieser","year":"2011","journal-title":"J. Proteome Res."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1093\/nar\/gkn923","article-title":"Bioinformatics enrichment tools: Paths toward the comprehen-sive functional analysis of large gene lists","volume":"37","author":"Huang","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_72","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1038\/nprot.2008.211","article-title":"Systematic and integrative analysis of large gene lists using DA-VID bioinformatics resources","volume":"4","author":"Huang","year":"2009","journal-title":"Nat. Protoc."},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"623","DOI":"10.1021\/acs.jproteome.8b00702","article-title":"Cytoscape StringApp: Network analysis and visualiza-tion of proteomics data","volume":"18","author":"Doncheva","year":"2019","journal-title":"J Proteome Res."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1038\/nmeth.3125","article-title":"Neuronal morphometry directly from bitmap images","volume":"11","author":"Ferreira","year":"2014","journal-title":"Nat. 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