{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T02:20:27Z","timestamp":1783390827812,"version":"3.54.6"},"reference-count":43,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["H2020-SCI-2016-2017"],"award-info":[{"award-number":["H2020-SCI-2016-2017"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Primary Sj\u00f6gren\u2019s syndrome (pSS) is a complex heterogeneous disease characterized by a wide spectrum of glandular and extra-glandular manifestations. In this pilot study, a SWATH-MS approach was used to monitor extracellular vesicles-enriched saliva (EVs) sub-proteome in pSS patients, to compare it with whole saliva (WS) proteome, and assess differential expressed proteins between pSS and healthy control EVs samples. Comparison between EVs and WS led to the characterization of compartment-specific proteins with a moderate degree of overlap. A total of 290 proteins were identified and quantified in EVs from healthy and pSS patients. Among those, 121 proteins were found to be differentially expressed in pSS, 82% were found to be upregulated, and 18% downregulated in pSS samples. The most representative functional pathways associated to the protein networks were related to immune-innate response, including several members of S100 protein family, annexin A2, resistin, serpin peptidase inhibitors, azurocidin, and CD14 monocyte differentiation antigen. Our results highlight the usefulness of EVs for the discovery of novel salivary-omic biomarkers and open novel perspectives in pSS for the identification of proteins of clinical relevance that could be used not only for the disease diagnosis but also to improve patients\u2019 stratification and treatment-monitoring. Data are available via ProteomeXchange with identifier PXD025649.<\/jats:p>","DOI":"10.3390\/ijms22094864","type":"journal-article","created":{"date-parts":[[2021,5,5]],"date-time":"2021-05-05T11:06:01Z","timestamp":1620212761000},"page":"4864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Characterization of Extracellular Vesicle Cargo in Sj\u00f6gren\u2019s Syndrome through a SWATH-MS Proteomics Approach"],"prefix":"10.3390","volume":"22","author":[{"given":"Francesco","family":"Finamore","sequence":"first","affiliation":[{"name":"Clinical Phisiology Institute-CNR, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Cecchettini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Rheumatology Unit, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3912-2753","authenticated-orcid":false,"given":"Elisa","family":"Ceccherini","sequence":"additional","affiliation":[{"name":"Clinical Phisiology Institute-CNR, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0067-2240","authenticated-orcid":false,"given":"Giovanni","family":"Signore","sequence":"additional","affiliation":[{"name":"Fondazione Pisana per la Scienza, S Giuliano Terme, 56017 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Ferro","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Rheumatology Unit, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3831-4200","authenticated-orcid":false,"given":"Silvia","family":"Rocchiccioli","sequence":"additional","affiliation":[{"name":"Clinical Phisiology Institute-CNR, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Baldini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, Rheumatology Unit, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,4]]},"reference":[{"key":"ref_1","first-page":"3","article-title":"One year in review 2019: Sj\u00f6gren\u2019s syndrome","volume":"37","author":"Cafaro","year":"2019","journal-title":"Clin. 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