{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T23:42:34Z","timestamp":1788997354353,"version":"build-2803163510"},"reference-count":74,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T00:00:00Z","timestamp":1620950400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["DFG STU 258\/7-1"],"award-info":[{"award-number":["DFG STU 258\/7-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005707","name":"Akademie f\u00fcr Tiergesundheit","doi-asserted-by":"publisher","award":["AfT DM 20160728"],"award-info":[{"award-number":["AfT DM 20160728"]}],"id":[{"id":"10.13039\/501100005707","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A therapeutic potential of the TRPA1 channel agonist cinnamaldehyde for use in inflammatory bowel disease is emerging, but the mechanisms are unclear. Semi-quantitative qPCR of various parts of the porcine gastrointestinal tract showed that mRNA for TRPA1 was highest in the colonic mucosa. In Ussing chambers, 1 mmol\u00b7L\u22121 cinnamaldehyde induced increases in short circuit current (\u0394Isc) and conductance (\u0394Gt) across the colon that were higher than those across the jejunum or after 1 mmol\u00b7L\u22121 thymol. Lidocaine, amiloride or bumetanide did not change the response. The application of 1 mmol\u00b7L\u22121 quinidine or the bilateral replacement of 120 Na+, 120 Cl\u2212 or 25 HCO3\u2212 reduced \u0394Gt, while the removal of Ca2+ enhanced \u0394Gt with \u0394Isc numerically higher. \u0394Isc decreased after 0.5 NPPB, 0.01 indometacin and the bilateral replacement of 120 Na+ or 25 HCO3\u2212. The removal of 120 Cl\u2212 had no effect. Cinnamaldehyde also activates TRPV3, but comparative measurements involving patch clamp experiments on overexpressing cells demonstrated that much higher concentrations are required. We suggest that cinnamaldehyde stimulates the secretion of HCO3\u2212 via apical CFTR and basolateral Na+-HCO3\u2212 cotransport, preventing acidosis and damage to the epithelium and the colonic microbiome. Signaling may involve the opening of TRPA1, depolarization of the epithelium and a rise in PGE2 following a lower uptake of prostaglandins via OATP2A1.<\/jats:p>","DOI":"10.3390\/ijms22105198","type":"journal-article","created":{"date-parts":[[2021,5,14]],"date-time":"2021-05-14T10:54:22Z","timestamp":1620989662000},"page":"5198","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["The TRPA1 Agonist Cinnamaldehyde Induces the Secretion of HCO3\u2212 by the Porcine Colon"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4478-1980","authenticated-orcid":false,"given":"David","family":"Manneck","sequence":"first","affiliation":[{"name":"Department of Veterinary Medicine, Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gisela","family":"Manz","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah-Sophie","family":"Braun","sequence":"additional","affiliation":[{"name":"PerformaNat GmbH, Hohentwielsteig 6, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Rosendahl","sequence":"additional","affiliation":[{"name":"PerformaNat GmbH, Hohentwielsteig 6, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9518-9237","authenticated-orcid":false,"given":"Friederike","family":"Stumpff","sequence":"additional","affiliation":[{"name":"Department of Veterinary Medicine, Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, Oertzenweg 19b, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"725","DOI":"10.1152\/physrev.00005.2019","article-title":"Mammalian Transient Receptor Potential TRPA1 Channels: From Structure to Disease","volume":"100","author":"Talavera","year":"2020","journal-title":"Physiol. 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