{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T06:05:04Z","timestamp":1771653904069,"version":"3.50.1"},"reference-count":51,"publisher":"MDPI AG","issue":"10","license":[{"start":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T00:00:00Z","timestamp":1621382400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","award":["HOMING PLUS\/2011-4\/10"],"award-info":[{"award-number":["HOMING PLUS\/2011-4\/10"]}],"id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2014\/15\/B\/NZ6\/03716"],"award-info":[{"award-number":["2014\/15\/B\/NZ6\/03716"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Inflammatory bowel disease is characterized by the infiltration of immune cells and chronic inflammation. The immune inhibitory receptor, CD200R, is involved in the downregulation of the activation of immune cells to prevent excessive inflammation. We aimed to define the role of CD200R ligand-CD200 in the experimental model of intestinal inflammation in conventionally-reared mice. Mice were given a dextran sodium sulfate solution in drinking water. Bodyweight loss was monitored daily and the disease activity index was calculated, and a histological evaluation of the colon was performed. TNF-\u03b1 production was measured in the culture of small fragments of the distal colon or bone marrow-derived macrophages (BMDMs) cocultured with CD200+ cells. We found that Cd200\u2212\/\u2212 mice displayed diminished severity of colitis when compared to WT mice. Inflammation significantly diminished CD200 expression in WT mice, particularly on vascular endothelial cells and immune cells. The co-culture of BMDMs with CD200+ cells inhibited TNF-\u03b1 secretion. In vivo, acute colitis induced by DSS significantly increased TNF-\u03b1 secretion in colon tissue in comparison to untreated controls. However, Cd200\u2212\/\u2212 mice secreted a similar level of TNF-\u03b1 to WT mice in vivo. CD200 regulates the severity of DSS-induced colitis in conventionally-reared mice. The presence of CD200+ cells decreases TNF-\u03b1 production by macrophages in vitro. However, during DDS-induced intestinal inflammation secretion of TNF-\u03b1 is independent of CD200 expression.<\/jats:p>","DOI":"10.3390\/ijms22105358","type":"journal-article","created":{"date-parts":[[2021,5,19]],"date-time":"2021-05-19T12:58:07Z","timestamp":1621429087000},"page":"5358","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The CD200 Regulates Inflammation in Mice Independently of TNF-\u03b1 Production"],"prefix":"10.3390","volume":"22","author":[{"given":"Katarzyna","family":"Tonecka","sequence":"first","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Agata","family":"Braniewska","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zofia","family":"Pilch","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuzanna","family":"Sas","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcin","family":"Skorzynski","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3680-0926","authenticated-orcid":false,"given":"Elisabetta","family":"Manuali","sequence":"additional","affiliation":[{"name":"Laboratory of Veterinary and Comparative Histopathology, Istituto Zooprofilattico Sperimentale Umbria e Marche \u201cTogo Rosati\u201d, 06126 Perugia, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4746-6505","authenticated-orcid":false,"given":"Tomasz P.","family":"Rygiel","sequence":"additional","affiliation":[{"name":"Department of Immunology, Medical University of Warsaw, 02-097 Warsaw, Poland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,19]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"91","DOI":"10.3748\/wjg.v20.i1.91","article-title":"Inflammatory Bowel Disease: Pathogenesis","volume":"20","author":"Zhang","year":"2014","journal-title":"World J. 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