{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T10:06:47Z","timestamp":1781690807650,"version":"3.54.5"},"reference-count":54,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Ministry of Science and Technology, Taiwan","award":["MOST 108-2628-B-002-013"],"award-info":[{"award-number":["MOST 108-2628-B-002-013"]}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST 108-2113-M-002-011"],"award-info":[{"award-number":["MOST 108-2113-M-002-011"]}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST 109-2628-B-002-037"],"award-info":[{"award-number":["MOST 109-2628-B-002-037"]}]},{"name":"Ministry of Science and Technology, Taiwan","award":["MOST 109-2113-M-002-003"],"award-info":[{"award-number":["MOST 109-2113-M-002-003"]}]},{"name":"National Taiwan University, Taiwan","award":["NTU-CDP-108L7861"],"award-info":[{"award-number":["NTU-CDP-108L7861"]}]},{"name":"National Taiwan University, Taiwan","award":["NTU-CDP-106R7867"],"award-info":[{"award-number":["NTU-CDP-106R7867"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Urate oxidase initiates the uric acid degradation pathways and is extensively used for protein drug development for gout therapy and serum uric acid diagnosis. We first present the biochemical and structural elucidation of a urate oxidase from the extremophile microorganism Deinococcus radiodurans (DrUox). From enzyme characterization, DrUox showed optimal catalytic ability at 30 \u00b0C and pH 9.0 with high stability under physiological conditions. Only the Mg2+ ion moderately elevated its activity, which indicates the characteristic of the cofactor-free urate oxidase family. Of note, DrUox is thermostable in mesophilic conditions. It retains almost 100% activity when incubated at 25 \u00b0C and 37 \u00b0C for 24 h. In this study, we characterized a thermostable urate oxidase, DrUox with high catalytic efficiency and thermal stability, which strengthens its potential for medical applications.<\/jats:p>","DOI":"10.3390\/ijms22115611","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T13:14:21Z","timestamp":1621948461000},"page":"5611","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Molecular Elucidation of a Urate Oxidase from Deinococcus radiodurans for Hyperuricemia and Gout Therapy"],"prefix":"10.3390","volume":"22","author":[{"given":"Yi-Chih","family":"Chiu","sequence":"first","affiliation":[{"name":"Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 115024, Taiwan"},{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting-Syuan","family":"Hsu","sequence":"additional","affiliation":[{"name":"Taipei First Girl High School, Taipei 10045, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen-Yu","family":"Huang","sequence":"additional","affiliation":[{"name":"Taipei First Girl High School, Taipei 10045, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0008-7383","authenticated-orcid":false,"given":"Chun-Hua","family":"Hsu","sequence":"additional","affiliation":[{"name":"Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei 115024, Taiwan"},{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"},{"name":"Institute of Biochemical Sciences, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"947","DOI":"10.1038\/nsb1197-947","article-title":"Crystal structure of the protein drug urate oxidase-inhibitor complex at 2.05 A resolution","volume":"4","author":"Bachet","year":"1997","journal-title":"Nat. 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