{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,7]],"date-time":"2025-11-07T09:41:30Z","timestamp":1762508490424,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T00:00:00Z","timestamp":1621900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Foundation of China","award":["Nos. 31872427","Nos. 31872428"],"award-info":[{"award-number":["Nos. 31872427","Nos. 31872428"]}]},{"name":"Natural Science Foundation of Chongqing","award":["cstc2018jcyjAX0528","cstc2020jscx-msxmX0045"],"award-info":[{"award-number":["cstc2018jcyjAX0528","cstc2020jscx-msxmX0045"]}]},{"name":"The Municipal Graduate Student Research Innovation Project of Chongqing","award":["No. CYB20116"],"award-info":[{"award-number":["No. CYB20116"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The trachea of insects is a tubular epithelia tissue that transports oxygen and other gases. It serves as a useful model for the studying of the cellular and molecular events involved in epithelial tube formation. Almost all of the extracellular matrix can be degraded by Matrix metalloproteinases (MMPs), which is closely related to the processes of development and regeneration. The regulation of trachea by MMPs is roughly known in previous studies, but the detailed regulation mechanism and involved gene function are not fully explored. In this article, we found MMP1 expressed highly during tracheal remodeling, and knocked out it makes the tracheal branch number reduced in Bombyx mori. In trachea of transgenic BmMMP1-KO silkworm, the space expanding of taenidium and epidermal cells and the structure of apical membrane were abnormal. To explore the underlying mechanism, we detected that DE-cadherin and Integrin \u03b21 were accumulated in trachea of transgenic BmMMP1-KO silkworm by immunohistochemistry. Moreover, 5-Bromo-2\u2032-Deoxyuridine (BrdU) labeling showed that knockout of BmMMP1 in silkworm inhibited tracheal cell proliferation, and BmMMP1 also regulated the proliferation and migration of BmNS cells. All of the results demonstrated that BmMMP1 regulates the development of the tracheal tissue by expanding the space of tracheal cuticles and increases the number of tracheal branches by degrading DE-cadherin and Integrin \u03b21.<\/jats:p>","DOI":"10.3390\/ijms22115618","type":"journal-article","created":{"date-parts":[[2021,5,25]],"date-time":"2021-05-25T22:02:23Z","timestamp":1621980143000},"page":"5618","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["A Matrix Metalloproteinase Mediates Tracheal Development in Bombyx mori"],"prefix":"10.3390","volume":"22","author":[{"given":"Yi","family":"Wei","sequence":"first","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Lin","family":"Zhou","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tai-Hang","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"},{"name":"Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Jiang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhan-Qi","family":"Dong","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"},{"name":"Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Hui","family":"Pan","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"},{"name":"Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China"},{"name":"Key Laboratory for Sericulture Functional Genomics and Biotechnology of Agricultural Ministry, Southwest University, Chongqing 400716, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"197","DOI":"10.1177\/10454411930040020401","article-title":"Matrix metalloproteinases: A review","volume":"4","author":"Moore","year":"1993","journal-title":"Crit. 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