{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:05:48Z","timestamp":1790809548620,"version":"4.1.0"},"reference-count":45,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T00:00:00Z","timestamp":1621987200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010198","name":"Ministerio de Econom\u00eda, Industria y Competitividad, Gobierno de Espa\u00f1a","doi-asserted-by":"publisher","award":["AGL2015\u201070140\u2010R"],"award-info":[{"award-number":["AGL2015\u201070140\u2010R"]}],"id":[{"id":"10.13039\/501100010198","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["PID2019\u2010107145RB\u2010I00"],"award-info":[{"award-number":["PID2019\u2010107145RB\u2010I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["BIO2017-86500-R"],"award-info":[{"award-number":["BIO2017-86500-R"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100019408","name":"Ministerio para la Transici\u00f3n Ecol\u00f3gica y el Reto Demogr\u00e1fico","doi-asserted-by":"publisher","award":["PRCV00820"],"award-info":[{"award-number":["PRCV00820"]}],"id":[{"id":"10.13039\/501100019408","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Horizon 2020","award":["731014"],"award-info":[{"award-number":["731014"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Embryonic implantation is a key step in the establishment of pregnancy. In the present work, we have carried out an in-depth proteomic analysis of the secretome (extracellular vesicles and soluble proteins) of two bovine blastocysts embryonic trophectoderm primary cultures (BBT), confirming different epithelial\u2013mesenchymal transition stages in these cells. BBT-secretomes contain early pregnancy-related proteins and angiogenic proteins both as cargo in EVs and the soluble fraction. We have demonstrated the functional transfer of protein-containing secretome between embryonic trophectoderm and maternal MSC in vitro using two BBT primary cultures eight endometrial MSC (eMSC) and five peripheral blood MSC (pbMSC) lines. We observed that eMSC and pbMSC chemotax to both the soluble fraction and EVs of the BBT secretome. In addition, in a complementary direction, we found that the pattern of expression of implantation proteins in BBT-EVs changes depending on: (i) their epithelial\u2013mesenchymal phenotype; (ii) as a result of the uptake of eMSC- or pbMSC-EV previously stimulated or not with embryonic signals (IFN-); (iii) because of the stimulation with the endometrial cytokines present in the uterine fluid in the peri-implantation period.<\/jats:p>","DOI":"10.3390\/ijms22115638","type":"journal-article","created":{"date-parts":[[2021,5,26]],"date-time":"2021-05-26T10:30:32Z","timestamp":1622025032000},"page":"5638","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Embryonic Trophectoderm Secretomics Reveals Chemotactic Migration and Intercellular Communication of Endometrial and Circulating MSCs in Embryonic Implantation"],"prefix":"10.3390","volume":"22","author":[{"given":"Alexandra","family":"Calle","sequence":"first","affiliation":[{"name":"Departamento de Reproducci\u00f3n Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00edctor","family":"Toribio","sequence":"additional","affiliation":[{"name":"CBM-SO, Instituto de Investigaci\u00f3n Sanitaria Princesa (IIS-IP), 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7484-2866","authenticated-orcid":false,"given":"Mar\u00eda","family":"Y\u00e1\u00f1ez-M\u00f3","sequence":"additional","affiliation":[{"name":"CBM-SO, Instituto de Investigaci\u00f3n Sanitaria Princesa (IIS-IP), 28049 Madrid, Spain"},{"name":"Departamento de Biolog\u00eda Molecular, UAM, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5868-2134","authenticated-orcid":false,"given":"Miguel \u00c1ngel","family":"Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Reproducci\u00f3n Animal, Instituto Nacional de Investigaci\u00f3n y Tecnolog\u00eda Agraria y Alimentaria (INIA), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1530\/REP-11-0364","article-title":"Expression of mesenchymal-related genes by the bovine trophectoderm following conceptus attachment to the endometrial epithelium","volume":"143","author":"Yamakoshi","year":"2012","journal-title":"Reproduction"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1016\/j.devcel.2014.03.005","article-title":"Transcriptional mechanisms link epithelial plasticity to adhesion and differentiation of epidermal progenitor cells","volume":"29","author":"Lee","year":"2014","journal-title":"Dev. 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