{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T15:41:29Z","timestamp":1793288489439,"version":"4.5.0"},"reference-count":55,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T00:00:00Z","timestamp":1622419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"State of Berlin","award":["Elsa Neumann Grant to S.K. Becker"],"award-info":[{"award-number":["Elsa Neumann Grant to S.K. Becker"]}]},{"DOI":"10.13039\/100005156","name":"Alexander von Humboldt-Stiftung","doi-asserted-by":"publisher","award":["Georg Forster Research Fellowship to M.A. Sandhu"],"award-info":[{"award-number":["Georg Forster Research Fellowship to M.A. Sandhu"]}],"id":[{"id":"10.13039\/100005156","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>At the onset of lactation, dairy cows suffer from insulin resistance, insulin deficiency or both, similar to human diabetes, resulting in lipolysis, ketosis and fatty liver. This work explored the combined effects of different levels of magnesium (0.1, 0.3, 1 and 3 mM) and insulin (25, 250 and 25,000 pM) on metabolic pathways and the expression of magnesium-responsive genes in a bovine adipocyte model. Magnesium starvation (0.1 mM) and low insulin (25 pM) independently decreased or tended to decrease the accumulation of non-polar lipids and uptake of the glucose analog 6-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-6-deoxyglucose (6-NBDG). Activity of glycerol 3-phosphate dehydrogenase (GPDH) was highest at 25 pM insulin and 3 mM magnesium. Expression of SLC41A1 and SLC41A3 was reduced at 0.1 mM magnesium either across insulin concentrations (SLC41A1) or at 250 pM insulin (SLC41A3). MAGT1 expression was reduced at 3 mM magnesium. NIPA1 expression was reduced at 3 mM and 0.1 mM magnesium at 25 and 250 pM insulin, respectively. Expression of SLC41A2, CNNM2, TRPM6 and TRPM7 was not affected. We conclude that magnesium promotes lipogenesis in adipocytes and inversely regulates the transcription of genes that increase vs. decrease cytosolic magnesium concentration. The induction of GAPDH activity by surplus magnesium at low insulin concentration can counteract excessive lipomobilization.<\/jats:p>","DOI":"10.3390\/ijms22115897","type":"journal-article","created":{"date-parts":[[2021,5,31]],"date-time":"2021-05-31T05:53:38Z","timestamp":1622440418000},"page":"5897","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["The Combined Influence of Magnesium and Insulin on Central Metabolic Functions and Expression of Genes Involved in Magnesium Homeostasis of Cultured Bovine Adipocytes"],"prefix":"10.3390","volume":"22","author":[{"given":"Sandra K.","family":"Becker","sequence":"first","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Sponder","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3051-7820","authenticated-orcid":false,"given":"Mansur A.","family":"Sandhu","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"},{"name":"Department of Veterinary Biomedical Sciences, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Trappe","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Kolisek","sequence":"additional","affiliation":[{"name":"Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4D, 03601 Martin, Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg R.","family":"Aschenbach","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Physiology, Freie Universit\u00e4t Berlin, 14163 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,5,31]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1016\/j.cimid.2011.01.004","article-title":"Lipid mobilization and inflammatory responses during the transition period of dairy cows","volume":"34","author":"Contreras","year":"2011","journal-title":"Comp. 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