{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T21:29:21Z","timestamp":1790544561579,"version":"4.1.0"},"reference-count":52,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP15K11056, JP15K11129, JP16K20616, JP18K17179, JP19K10117, and JP19H03833"],"award-info":[{"award-number":["JP15K11056, JP15K11129, JP16K20616, JP18K17179, JP19K10117, and JP19H03833"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"the Tokyo Dental College Research Branding Project (Multidisciplinary Research Center for Jaw Disease (MRCJD): Achieving Longevity and Sustainability by Comprehensive Reconstruction of Oral and Maxillofacial functions)","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"the Science Research Promotion Fund from the Promotion and Mutual Aid Corporation for Pri-vate Schools of Japan","award":["NA"],"award-info":[{"award-number":["NA"]}]},{"name":"grant by subsidy for current expenditures to private institutions of higher education from MEXT of Japan","award":["NA"],"award-info":[{"award-number":["NA"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The ionotropic P2X receptor, P2X7, is believed to regulate and\/or generate nociceptive pain, and pain in several neuropathological diseases. Although there is a known relationship between P2X7 receptor activity and pain sensing, its detailed functional properties in trigeminal ganglion (TG) neurons remains unclear. We examined the electrophysiological and pharmacological characteristics of the P2X7 receptor and its functional coupling with other P2X receptors and pannexin-1 (PANX1) channels in primary cultured rat TG neurons, using whole-cell patch-clamp recordings. Application of ATP and Bz-ATP induced long-lasting biphasic inward currents that were more sensitive to extracellular Bz-ATP than ATP, indicating that the current was carried by P2X7 receptors. While the biphasic current densities of the first and second components were increased by Bz-ATP in a concentration dependent manner; current duration was only affected in the second component. These currents were significantly inhibited by P2X7 receptor antagonists, while only the second component was inhibited by P2X1, 3, and 4 receptor antagonists, PANX1 channel inhibitors, and extracellular ATPase. Taken together, our data suggests that autocrine or paracrine signaling via the P2X7-PANX1-P2X receptor\/channel complex may play important roles in several pain sensing pathways via long-lasting neuronal activity driven by extracellular high-concentration ATP following tissue damage in the orofacial area.<\/jats:p>","DOI":"10.3390\/ijms22115978","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T15:58:44Z","timestamp":1622563124000},"page":"5978","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Functional Coupling between the P2X7 Receptor and Pannexin-1 Channel in Rat Trigeminal Ganglion Neurons"],"prefix":"10.3390","volume":"22","author":[{"given":"Hiroyuki","family":"Inoue","sequence":"first","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"},{"name":"Department of Dental Anesthesiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7521-8096","authenticated-orcid":false,"given":"Hidetaka","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"},{"name":"Department of Dental Anesthesiology, Kanagawa Dental University, Kanagawa 238-8580, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wataru","family":"Ofusa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sadao","family":"Oyama","sequence":"additional","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Kimura","sequence":"additional","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuya","family":"Ichinohe","sequence":"additional","affiliation":[{"name":"Department of Dental Anesthesiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyuki","family":"Shibukawa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Tokyo Dental College, Tokyo 101-0061, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.1038\/387505a0","article-title":"Distinct ATP Receptors on Pain-Sensing and Stretch-Sensing Neurons","volume":"387","author":"Cook","year":"1997","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1152\/physrev.00015.2002","article-title":"Molecular Physiology of P2X Receptors","volume":"82","author":"North","year":"2002","journal-title":"Physiol. 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