{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T22:52:33Z","timestamp":1793659953550,"version":"4.5.1"},"reference-count":60,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T00:00:00Z","timestamp":1622505600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Nanozymes, nanomaterials with enzyme-like activities, are becoming powerful competitors and potential substitutes for natural enzymes because of their excellent performance. Nanozymes offer better structural stability over their respective natural enzymes. In consequence, nanozymes exhibit promising applications in different fields such as the biomedical sector (in vivo diagnostics\/and therapeutics) and the environmental sector (detection and remediation of inorganic and organic pollutants). Prussian blue nanoparticles and their analogues are metal\u2013organic frameworks (MOF) composed of alternating ferric and ferrous irons coordinated with cyanides. Such nanoparticles benefit from excellent biocompatibility and biosafety. Besides other important properties, such as a highly porous structure, Prussian blue nanoparticles show catalytic activities due to the iron atom that acts as metal sites for the catalysis. The different states of oxidation are responsible for the multicatalytic activities of such nanoparticles, namely peroxidase-like, catalase-like, and superoxide dismutase-like activities. Depending on the catalytic performance, these nanoparticles can generate or scavenge reactive oxygen species (ROS).<\/jats:p>","DOI":"10.3390\/ijms22115993","type":"journal-article","created":{"date-parts":[[2021,6,1]],"date-time":"2021-06-01T15:58:44Z","timestamp":1622563124000},"page":"5993","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":166,"title":["Prussian Blue: A Nanozyme with Versatile Catalytic Properties"],"prefix":"10.3390","volume":"22","author":[{"given":"Joan","family":"Estelrich","sequence":"first","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Avda Joan XXIII, 27-31, 08028 Barcelona, Catalonia, Spain"},{"name":"Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Avda. Diagonal 645, 08028 Barcelona, Catalonia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6232-9886","authenticated-orcid":false,"given":"M. Ant\u00f2nia","family":"Busquets","sequence":"additional","affiliation":[{"name":"Department of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, University of Barcelona, Avda Joan XXIII, 27-31, 08028 Barcelona, Catalonia, Spain"},{"name":"Institute of Nanoscience and Nanotechnology (IN2UB), University of Barcelona, Avda. Diagonal 645, 08028 Barcelona, Catalonia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1021\/acs.chemrev.8b00672","article-title":"Nanozymes: Classification, catalytic mechanisms, activity regulation, and applications","volume":"119","author":"Huang","year":"2019","journal-title":"Chem. Rev."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2190","DOI":"10.1021\/acs.accounts.9b00140","article-title":"Nanozymes: From new concepts, mechanisms, and standards to applications","volume":"52","author":"Liang","year":"2019","journal-title":"Acc. Chem. Res."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Stasyuk, S., Smutok, O., Demkiv, O., Prokopiv, T., Gayda, G., Nisnevitch, M., and Gonchar, M. (2020). Synthesis, catalytic properties and application in biosensorics of nanozymes and electronanocatalysts: A review. Sensors, 20.","DOI":"10.3390\/s20164509"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1038\/nnano.2007.260","article-title":"Intrinsic peroxidase-like activity of ferromagnetic nanoparticles","volume":"2","author":"Gao","year":"2007","journal-title":"Nat. Nanotechnol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.mattod.2020.08.020","article-title":"Structure and activity of nanozymes: Inspirations for de novo design of nanozymes","volume":"41","author":"Wang","year":"2020","journal-title":"Mater. Today"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1509","DOI":"10.1345\/aph.1E024","article-title":"Soluble or insoluble Prussian blue for radiocesium and thallium poisoning","volume":"38","author":"Thompson","year":"2004","journal-title":"Ann. Pharmacother."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1537","DOI":"10.1039\/c2gc35079c","article-title":"Preparation of electrochromic Prussian blue nanoparticles dispersible into various solvents for realisation of printed electronics","volume":"14","author":"Ishizaki","year":"2012","journal-title":"Green Chem."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1021\/ed085p612","article-title":"Prussian blue: Artists\u2019 pigment and chemists\u2019 sponge","volume":"85","author":"Ware","year":"2008","journal-title":"J. Chem. Educ."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Guari, Y., and Larionova, J. (2019). Prussian Blue-Type Nanoparticles and Nanocomposites. Synthesis, Devices and Applications, Pan Stanford.","DOI":"10.1201\/9780429024740"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1413","DOI":"10.1016\/j.drudis.2020.05.014","article-title":"Prussian blue nanoparticles: Synthesis, surface modification and biomedical applications","volume":"25","author":"Busquets","year":"2020","journal-title":"Drug Discov. Today"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"85","DOI":"10.3390\/applnano2020008","article-title":"Prussian blue and its analogs as a novel nanostructured antibacterial materials","volume":"2","author":"Taglietti","year":"2021","journal-title":"Appl. Nano"},{"key":"ref_12","unstructured":"(2013). WHO Model List of Essential Medicines, World Health Organization. [3rd ed.]. Available online: https:\/\/www.who.int\/groups\/expert-committee-on-selection-and-use-of-essential-medicines\/essential-medicines-lists."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Estelrich, J., and Busquets, M.A. (2021). Prussian blue: A safe pigment with zeolitic-like activity. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22020780"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"662","DOI":"10.1021\/acs.analchem.9b04931","article-title":"Integrating Prussian blue analog-based nanozyme and online visible light absorption approach for continuous hydrogen sulfide monitoring in brains of living rats","volume":"92","author":"Wang","year":"2020","journal-title":"Anal. Chem."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2812","DOI":"10.1021\/acs.nanolett.8b04729","article-title":"Hollow Prussian blue nanozymes drive neuroprotection against ischemic stroke via attenuating oxidative stress, counteracting inflammation, and suppressing cell apoptosis","volume":"19","author":"Zhang","year":"2019","journal-title":"Nano Lett."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"3387","DOI":"10.1039\/c2ce25040c","article-title":"Size- and shape-controlled synthesis of Prussian blue nanoparticles by a polyvinylpyrrolidone-assisted crystallization process","volume":"14","author":"Ming","year":"2012","journal-title":"CrystEngComm"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1246\/bcsj.20180368","article-title":"Nanoarchitectonics: A new materials horizon for Prussian blue and its analogues","volume":"92","author":"Azhar","year":"2019","journal-title":"Bull. Chem. Soc. Jpn."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Dacarro, G., Taglietti, A., and Pallavicini, P. (2018). Prussian blue nanoparticles as a versatile photothermal tool. Molecules, 23.","DOI":"10.3390\/molecules23061414"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"35068","DOI":"10.1021\/acsami.0c18357","article-title":"Achieving ultrasmall Prussian blue nanoparticles as high-performance biomedical agents with multifunctions","volume":"12","author":"Qin","year":"2020","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"113066","DOI":"10.1016\/j.molliq.2020.113066","article-title":"A colloid approach to decorate latex particles with Prussian blue nanozymes","volume":"309","author":"Alsharif","year":"2020","journal-title":"J. Mol. Liq."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1016\/j.inoche.2009.10.015","article-title":"Biocompatible Prussian blue nanoparticles: Preparation, stability, cytotoxicity, and potential use as an MRI contrast agent","volume":"13","author":"Shokouhimehr","year":"2010","journal-title":"Inorg. Chem. Commun."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"491","DOI":"10.3389\/fchem.2020.00491","article-title":"Reversible inhibition of iron oxide nanozyme by guanidine chloride","volume":"8","author":"Mo","year":"2020","journal-title":"Front. Chem."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"89","DOI":"10.59566\/IJBS.2008.4089","article-title":"Free radicals, antioxidants in disease and health","volume":"4","author":"He","year":"2008","journal-title":"Int. J. Biomed. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"11302","DOI":"10.1021\/jacs.8b05223","article-title":"Catalytically synthesized Prussian blue nanoparticles defeating natural enzyme peroxidase","volume":"140","author":"Komkova","year":"2018","journal-title":"J. Am. Chem. Soc."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"5110","DOI":"10.1039\/c0jm00174k","article-title":"Prussian blue modified iron oxide magnetic nanoparticles and their high peroxidase-like activity","volume":"20","author":"Zhang","year":"2010","journal-title":"J. Mater. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"362","DOI":"10.1016\/j.talanta.2013.12.028","article-title":"Prussian blue nanoparticles as peroxidase mimetics for sensitive colorimetric detection of hydrogen peroxide and glucose","volume":"120","author":"Zhang","year":"2014","journal-title":"Talanta"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1007\/s00604-015-1697-z","article-title":"Catalytic nanocrystalline coordination polymers as an efficient peroxidase mimic for labeling and optical immunoassays","volume":"183","author":"Peterek","year":"2016","journal-title":"Microchim. Acta"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4958","DOI":"10.1021\/acs.nanolett.7b02102","article-title":"Mimicking peroxidase activities with Prussian blue nanoparticles and their cyanometallate structural analogues","volume":"17","author":"Kozell","year":"2017","journal-title":"Nano Lett."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"4015","DOI":"10.1021\/acs.nanolett.8b01522","article-title":"Catalyzed and electrocatalyzed oxidation of L-tyrosine and L-phenylalanine to dopachrome by nanozymes","volume":"18","author":"Hou","year":"2018","journal-title":"Nano Lett."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"2348","DOI":"10.1021\/acs.analchem.7b04883","article-title":"Prussian blue nanoparticles as a catalytic label in sandwich nanozyme-linked immunosorbent assay","volume":"90","author":"Farka","year":"2018","journal-title":"Anal. Chem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5860","DOI":"10.1021\/jacs.5b12070","article-title":"Prussian blue nanoparticles as multienzyme mimetics and reactive oxygen species scavengers","volume":"138","author":"Zhang","year":"2016","journal-title":"J. Am. Chem. Soc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1166\/jnn.2013.6871","article-title":"Prussian blue modified ferritin as peroxidase mimetics and its applications in biological detection","volume":"13","author":"Zhang","year":"2013","journal-title":"J. Nanosci. Nanotechnol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1016\/j.colsurfa.2015.11.035","article-title":"Polystyrene@Au@prussian blue nanocomposites with enzyme-like activity and their application in glucose detection","volume":"490","author":"Zhang","year":"2016","journal-title":"Colloids Surfaces A"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1016\/j.colsurfb.2018.01.010","article-title":"Sparks fly between ascorbic acid and iron-based nanozymes: A study on Prussian blue nanoparticles","volume":"163","author":"Zhang","year":"2018","journal-title":"Colloids Surfaces B"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"98215","DOI":"10.1039\/C5RA18589K","article-title":"Prussian blue modified metal-organic framework MIL-101(Fe) with intrinsic peroxidase-like catalytic activity as a colorimetric biosensing platform","volume":"5","author":"Cui","year":"2015","journal-title":"RSC Adv."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"8802","DOI":"10.1039\/C5NJ01744K","article-title":"PB@Co3O4 nanoparticles as both oxidase and peroxidase mimics and their application for colorimetric detection of glutathione","volume":"39","author":"Yang","year":"2015","journal-title":"New J. Chem."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"15556","DOI":"10.1021\/acs.iecr.0c02154","article-title":"Self-reducing Prussian blue on Ti3C2Tx MXene nanosheets as a dual-functional nanohybrid for hydrogen peroxide and pesticide sensing","volume":"59","author":"He","year":"2020","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3017","DOI":"10.1021\/acsnano.9b07886","article-title":"A hybrid VOx incorporated hexacyanoferrate nanostructured hydrogel as a multienzyme mimetic via cascade reactions","volume":"14","author":"Sahar","year":"2020","journal-title":"ACS Nano"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3951","DOI":"10.1039\/C8AY01456F","article-title":"Dual function hollow structures mesoporous Prussian blue mesocrystals for glucose biosensors","volume":"10","author":"Zheng","year":"2018","journal-title":"Anal. Methods"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1007\/s00604-019-3826-6","article-title":"Hollow Prussian blue nanocubes as peroxidase mimetic and enzyme carriers for colorimetric determination of ethanol","volume":"186","author":"Wang","year":"2019","journal-title":"Microchim. Acta"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"12161","DOI":"10.1021\/acs.iecr.6b02953","article-title":"Facile synthesis of Prussian blue\/hollow polypyrrole nanocomposites for enhanced hydrogen peroxide sensing","volume":"55","author":"Yang","year":"2016","journal-title":"Ind. Eng. Chem. Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"4905","DOI":"10.1007\/s12274-018-2079-8","article-title":"Prussian blue with intrinsec heme-like structure as peroxidase enzyme","volume":"11","author":"Chen","year":"2018","journal-title":"Nano Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"5189","DOI":"10.1021\/acsnano.0c10388","article-title":"Platinum-doped Prussian blue nanozymes for multiwavelength bioimaging guided photothermal therapy of tumor and anti-inflammation","volume":"15","author":"Li","year":"2021","journal-title":"ACS Nano"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2858","DOI":"10.1021\/acsnano.8b00309","article-title":"Engineering of a sized biocatalyst for combined tumor starvation and low-temperature photothermal therapy","volume":"12","author":"Zhou","year":"2018","journal-title":"ACS Nano"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"5205","DOI":"10.1002\/adma.201202367","article-title":"A hydrogen peroxide-responsive O2 nanogenerator for ultrasound and magnetic resonance dual modality imaging","volume":"24","author":"Yang","year":"2012","journal-title":"Adv. Mater."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Gao, X., Wang, Q., Cheng, C., Lin, S., Lin, T., Liu, C., and Han, X. (2020). The application of Prussian blue nanoparticles in tumor diagnosis and treatment. Sensors, 20.","DOI":"10.3390\/s20236905"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"44410","DOI":"10.1021\/acsami.7b17022","article-title":"Erythrocyte-membrane-coated Prussian blue\/manganese dioxide nanoparticles as H2O2-responsive oxygen generators to enhance cancer chemotherapy\/photothermal therapy","volume":"9","author":"Peng","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"13875","DOI":"10.1021\/acsami.7b01365","article-title":"\u201cOne-for-all\u201d-type, biodegradable Prussian blue\/manganese dioxide hybrid nanocrystal for trimodal imaging-guided photothermal therapy and oxygen regulation of breast cancer","volume":"9","author":"Peng","year":"2017","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"6049","DOI":"10.1016\/S0021-9258(18)63504-5","article-title":"Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein)","volume":"244","author":"McCord","year":"1969","journal-title":"J. Biol. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"276","DOI":"10.1016\/0003-2697(71)90370-8","article-title":"Superoxide dismutase\u2014Improved assays and an assay applicable to acrylamide gels","volume":"44","author":"Beaucham","year":"1971","journal-title":"Anal. Biochem."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1039\/CT8946500899","article-title":"Oxidation of tartaric acid in presence of iron","volume":"65","author":"Fenton","year":"1894","journal-title":"J. Chem. Soc. Trans."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1007\/s10311-009-0242-x","article-title":"Degradation of dye rhodamine B under visible irradiation with Prussian blue as a photo-Fenton reagent","volume":"9","author":"Liu","year":"2009","journal-title":"Environm. Chem. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1039\/C4CY00947A","article-title":"Prussian blue\/TiO2 nanocomposites as a heterogeneous photo-Fenton catalyst for degradation of organic pollutants in water","volume":"5","author":"Li","year":"2015","journal-title":"Catal. Sci. Technol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.apcatb.2015.05.033","article-title":"Excellent photo-Fenton catalysts of Fe-Co Prussian blue analogues and their reaction mechanism study","volume":"179","author":"Li","year":"2015","journal-title":"Appl. Catal. B"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1016\/j.catcom.2016.01.004","article-title":"Hydrazine drastically promoted Fenton oxidation of bisphenol A catalysed by a Fe III-Co Prussian blue analogue","volume":"77","author":"Li","year":"2016","journal-title":"Catal. Commun."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"17568","DOI":"10.1039\/C5CC06281K","article-title":"Three-dimensional hierarchical Prussian blue composed of ultrathin nanosheets: Enhanced hetero-catalytic and adsorption properties","volume":"51","author":"Bu","year":"2015","journal-title":"Chem. Commun."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1174","DOI":"10.1021\/acsami.8b14987","article-title":"Prussian blue microcrystals with morphology evolution as a high-performance photo-Fenton catalyst for degradation of organic pollutants","volume":"11","author":"Wang","year":"2019","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.redox.2016.10.010","article-title":"Tumor cells have decreased ability to metabolize H2O2: Implications for pharmacological ascorbate to cancer therapy","volume":"10","author":"Doskey","year":"2016","journal-title":"Redox Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"26108","DOI":"10.1021\/acsami.8b10345","article-title":"Prussian blue nanozyme with multienzyme activity reduces colitis in mice","volume":"10","author":"Zhao","year":"2018","journal-title":"ACS Appl. Mater. Interfaces"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2843","DOI":"10.7150\/thno.33727","article-title":"Nanozyme-mediated catalytic nanotherapy for inflammatory bowel disease","volume":"9","author":"Zhao","year":"2019","journal-title":"Theranostics"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/11\/5993\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:09:56Z","timestamp":1760162996000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/11\/5993"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,1]]},"references-count":60,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ijms22115993"],"URL":"https:\/\/doi.org\/10.3390\/ijms22115993","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,1]]}}}