{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T02:12:11Z","timestamp":1785291131561,"version":"3.55.0"},"reference-count":62,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T00:00:00Z","timestamp":1623110400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fund for Scientific Research of Flanders","award":["C16\/17\/010"],"award-info":[{"award-number":["C16\/17\/010"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The airways of patients with primary ciliary dyskinesia (PCD) contain persistently elevated neutrophil numbers and CXCL8 levels. Despite their abundance, neutrophils fail to clear the airways from bacterial infections. We investigated whether neutrophil functions are altered in patients with PCD. Neutrophils from patients and healthy controls (HC) were isolated from peripheral blood and exposed to various bacterial stimuli or cytokines. Neutrophils from patients with PCD were less responsive to low levels of fMLF in three different chemotaxis assays (p &lt; 0.05), but expression of the fMLF receptors was unaltered. PCD neutrophils showed normal phagocytic function and expression of adhesion molecules. However, PCD neutrophils produced less reactive oxygen species upon stimulation with bacterial products or cytokines compared to HC neutrophils (p &lt; 0.05). Finally, the capacity to release DNA, as observed during neutrophil extracellular trap formation, seemed to be reduced in patients with PCD compared to HC (p = 0.066). These results suggest that peripheral blood neutrophils from patients with PCD, in contrast to those of patients with cystic fibrosis or COPD, do not show features of over-activation, neither on baseline nor after stimulation. If these findings extend to lung-resident neutrophils, the reduced neutrophil activity could possibly contribute to the recurrent respiratory infections in patients with PCD.<\/jats:p>","DOI":"10.3390\/ijms22126172","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T12:08:55Z","timestamp":1623154135000},"page":"6172","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["The Antimicrobial Activity of Peripheral Blood Neutrophils Is Altered in Patients with Primary Ciliary Dyskinesia"],"prefix":"10.3390","volume":"22","author":[{"given":"Maaike","family":"Cockx","sequence":"first","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6910-5797","authenticated-orcid":false,"given":"Marfa","family":"Blanter","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieke","family":"Gouwy","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pieter","family":"Ruytinx","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Abouelasrar Salama","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Knoops","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"No\u00ebmie","family":"P\u00f6rtner","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lotte","family":"Vanbrabant","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2653-5885","authenticated-orcid":false,"given":"Natalie","family":"Lorent","sequence":"additional","affiliation":[{"name":"Pneumology and Cystic Fibrosis Unit, Department of Pneumology, University Hospitals Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mieke","family":"Boon","sequence":"additional","affiliation":[{"name":"Pediatric Pneumology and Cystic Fibrosis Unit, Department of Pediatrics, University Hospitals Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofie","family":"Struyf","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Immunology, Department of Microbiology, Immunology and Transplantation, Rega Institute for Medical Research, University of Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1007\/s00431-012-1785-6","article-title":"Primary ciliary dyskinesia, an orphan disease","volume":"172","author":"Boon","year":"2013","journal-title":"Eur. J. Pediatrc."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1136\/adc.2006.096958","article-title":"Primary ciliary dyskinesia: Current state of the art","volume":"92","author":"Bush","year":"2007","journal-title":"Arch. Dis. Child."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"913","DOI":"10.1164\/rccm.201301-0059CI","article-title":"Primary Ciliary Dyskinesia. Recent Advances in Diagnostics, Genetics, and Characterization of Clinical Disease","volume":"188","author":"Knowles","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1517\/21678707.2015.989212","article-title":"Genetics, diagnosis and future treatment strategies for primary ciliary dyskinesia","volume":"3","author":"Daniels","year":"2015","journal-title":"Expert Opin. Orphan Drugs"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"67","DOI":"10.2147\/TACG.S127129","article-title":"Primary ciliary dyskinesia: Mechanisms and management","volume":"10","author":"Damseh","year":"2017","journal-title":"Appl. Clin. Genet."},{"key":"ref_6","first-page":"39","article-title":"Treatment recommendations in Primary Ciliary Dyskinesia","volume":"18","author":"Polineni","year":"2016","journal-title":"Paediatr. Respir. Rev."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"135","DOI":"10.3389\/fped.2017.00135","article-title":"Primary Ciliary Dyskinesia: An Update on Clinical Aspects, Genetics, Diagnosis, and Future Treatment Strategies","volume":"5","author":"Mirra","year":"2017","journal-title":"Front. Pediatr."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1007\/s11136-017-1564-y","article-title":"The patient\u2019s experience of primary ciliary dyskinesia: A systematic review","volume":"26","author":"Behan","year":"2017","journal-title":"Qual. Life Res."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"118","DOI":"10.1378\/chest.129.1.118","article-title":"Mucus Properties in Children with Primary Ciliary Dyskinesia: Comparison with cystic fibrosis","volume":"129","author":"Bush","year":"2006","journal-title":"Chest"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1183\/13993003.01390-2015","article-title":"Changes in airway inflammation during pulmonary exacerbations in patients with cystic fibrosis and primary ciliary dyskinesia","volume":"47","author":"Ratjen","year":"2016","journal-title":"Eur. Respir. J."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"961","DOI":"10.1164\/rccm.201509-1818OC","article-title":"Acute Respiratory Distress Syndrome Neutrophils Have a Distinct Phenotype and Are Resistant to Phosphoinositide 3-Kinase Inhibition","volume":"194","author":"Juss","year":"2016","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"531","DOI":"10.1165\/rcmb.2012-0492TR","article-title":"The Neutrophil in Chronic Obstructive Pulmonary Disease. Too Little, Too Late or Too Much, Too Soon?","volume":"48","author":"Hoenderdos","year":"2013","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"554","DOI":"10.1080\/01902140902777490","article-title":"Immune responses of airway neutrophils are impaired in asthma","volume":"35","author":"Baines","year":"2009","journal-title":"Exp. Lung Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"312","DOI":"10.1038\/cmi.2017.118","article-title":"Chemoattractants and cytokines in primary ciliary dyskinesia and cystic fibrosis: Key players in chronic respiratory diseases","volume":"15","author":"Cockx","year":"2018","journal-title":"Cell. Mol. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1016\/j.jcf.2015.03.003","article-title":"Inflammation in cystic fibrosis lung disease: Pathogenesis and therapy","volume":"14","author":"Cantin","year":"2015","journal-title":"J. Cyst. Fibros."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"16","DOI":"10.1097\/MOH.0000000000000009","article-title":"The role of neutrophils in cystic fibrosis","volume":"21","author":"Gifford","year":"2014","journal-title":"Curr. Opin. Hematol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1283","DOI":"10.1084\/jem.20122220","article-title":"Diverse novel functions of neutrophils in immunity, inflammation, and beyond","volume":"210","year":"2013","journal-title":"J. Exp. Med."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1111\/nyas.12445","article-title":"Regulation of immune responses by neutrophils","volume":"1319","author":"Wang","year":"2014","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1612","DOI":"10.1172\/JCI84538","article-title":"Neutrophil extracellular traps\u2014The dark side of neutrophils","volume":"126","author":"Borregaard","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1007\/s00281-013-0374-8","article-title":"Neutrophils in innate and adaptive immunity","volume":"35","author":"Jaillon","year":"2013","journal-title":"Semin. Immunopathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1016\/j.critrevonc.2018.10.003","article-title":"Congenital neutropenia and primary immunodeficiency diseases","volume":"133","author":"Spoor","year":"2019","journal-title":"Crit. Rev. Oncol. Hematol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1182\/asheducation-2016.1.43","article-title":"Primary immune deficiencies with defects in neutrophil function","volume":"2016","author":"Dinauer","year":"2016","journal-title":"Hematol. Am. Soc. Hematol. Educ. Program."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1601090","DOI":"10.1183\/13993003.01090-2016","article-title":"European Respiratory Society guidelines for the diagnosis of primary ciliary dyskinesia","volume":"49","author":"Lucas","year":"2017","journal-title":"Eur. Respir. J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1159\/000363242","article-title":"Cystic Fibrosis Sputum DNA Has NETosis Characteristics and Neutrophil Extracellular Trap Release Is Regulated by Macrophage Migration-Inhibitory Factor","volume":"6","author":"Dwyer","year":"2014","journal-title":"J. Innate Immun."},{"key":"ref_25","first-page":"1","article-title":"Advanced Role of Neutrophils in Common Respiratory Diseases","volume":"2017","author":"Liu","year":"2017","journal-title":"J. Immunol. Res."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1155\/2011\/139493","article-title":"Analysis of Sputum Markers in the Evaluation of Lung Inflammation and Functional Impairment in Symptomatic Smokers and COPD Patients","volume":"31","author":"Paone","year":"2011","journal-title":"Dis. Markers"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"601","DOI":"10.1016\/j.intimp.2004.11.007","article-title":"Effect of cystic fibrosis exacerbations on neutrophil function","volume":"5","author":"Brockbank","year":"2005","journal-title":"Int. Immunopharmacol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1043","DOI":"10.1016\/S0140-6736(87)91476-0","article-title":"Neutrophils from subjects with chronic obstructive lung disease show enhanced chemotaxis and extracellular proteolysis","volume":"2","author":"Burnett","year":"1987","journal-title":"Lancet"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1111\/resp.12657","article-title":"Differential neutrophil activation in viral infections: Enhanced TLR-7\/8-mediated CXCL8 release in asthma","volume":"21","author":"Tang","year":"2016","journal-title":"Respirology"},{"key":"ref_30","unstructured":"Konstan, M.W. (2019, February 28). Clinical Trials Gov 2018-02-23 2006; NCT00322868, Available online: https:\/\/clinicaltrials.gov\/ct2\/search."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Cockx, M., Gouwy, M., Godding, V., De Boeck, K., Van Damme, J., Boon, M., and Struyf, S. (2017). Neutrophils from Patients with Primary Ciliary Dyskinesia Display Reduced Chemotaxis to CXCR2 Ligands. Front. Immunol., 8.","DOI":"10.3389\/fimmu.2017.01126"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3346\/jkms.2003.18.1.36","article-title":"Chemotaxis of Blood Neutrophils from Patients with Primary Ciliary Dyskinesia","volume":"18","author":"Koh","year":"2003","journal-title":"J. Korean Med. Sci."},{"key":"ref_33","first-page":"838","article-title":"Neutrophil function in the immotile cilia syndrome","volume":"99","author":"Corkey","year":"1982","journal-title":"J. Lab. Clin. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1016\/j.cellsig.2007.11.008","article-title":"Tumor necrosis factor-alpha (TNF-\u03b1) induces integrin CD11b\/CD18 (Mac-1) up-regulation and migration to the CC chemokine CCL3 (MIP-1\u03b1) on human neutrophils through defined signalling pathways","volume":"20","author":"Montecucco","year":"2008","journal-title":"Cell. Signal."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"678","DOI":"10.1038\/nri2156","article-title":"Getting to the site of inflammation: The leukocyte adhesion cascade updated","volume":"7","author":"Ley","year":"2007","journal-title":"Nat. Rev. Immunol."},{"key":"ref_36","first-page":"409","article-title":"Leukocyte locomotory function in children with the immotile cilia syndrome","volume":"41","author":"Antonelli","year":"1986","journal-title":"Helv. Paediatr. Acta"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1001\/archotol.1990.01870040087020","article-title":"Characterization of a Chemotactic Defect in Patients with Kartagener Syndrome","volume":"116","author":"Walter","year":"1990","journal-title":"Arch. Otolaryngol. Head Neck Surg."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Forsman, H., \u00d6nnheim, K., Andr\u00e9asson, E., Christenson, K., Karlsson, A., Bylund, J., and Dahlgren, C. (2013). Reactivation of Desensitized Formyl Peptide Receptors by Platelet Activating Factor: A Novel Receptor Cross Talk Mechanism Regulating Neutrophil Superoxide Anion Production. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0060169"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"29750","DOI":"10.1074\/jbc.M110.119388","article-title":"Posttranslational Modification of the NH2-terminal Region of CXCL5 by Proteases or Peptidylarginine Deiminases (PAD) Differently Affects Its Biological Activity","volume":"285","author":"Mortier","year":"2010","journal-title":"J. Biol. Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"169","DOI":"10.1189\/jlb.0413219","article-title":"Expression profile of cord blood neutrophils and dysregulation of HSPA1A and OLR1 upon challenge by bacterial peptidoglycan","volume":"95","author":"Fong","year":"2014","journal-title":"J. Leukoc. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1407","DOI":"10.1016\/S0002-9440(10)65009-2","article-title":"Cystic Fibrosis Transmembrane Conductance Regulator Does Not Affect Neutrophil Migration across Cystic Fibrosis Airway Epithelial Monolayers","volume":"156","author":"Pizurki","year":"2000","journal-title":"Am. J. Pathol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"756","DOI":"10.1164\/ajrccm.157.3.9704008","article-title":"Neutrophil Adhesion Molecule Surface Expression and Responsiveness in Cystic Fibrosis","volume":"157","author":"Russell","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1136\/thx.56.6.432","article-title":"Enhanced neutrophil response in chronic obstructive pulmonary disease","volume":"56","author":"Noguera","year":"2001","journal-title":"Thorax"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1664","DOI":"10.1164\/ajrccm.158.5.9712092","article-title":"Expression of Adhesion Molecules and G Proteins in Circulating Neutrophils in Chronic Obstructive Pulmonary Disease","volume":"158","author":"Noguera","year":"1998","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1465-9921-7-59","article-title":"Blood neutrophil activation markers in severe asthma: Lack of inhibition by prednisolone therapy","volume":"7","author":"Mann","year":"2006","journal-title":"Respir. Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1111\/j.1365-2249.2005.02893.x","article-title":"Reduced iC3b-mediated phagocytotic capacity of pulmonary neutrophils in cystic fibrosis","volume":"142","author":"Morris","year":"2005","journal-title":"Clin. Exp. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1136\/thoraxjnl-2017-210134","article-title":"Delayed neutrophil apoptosis enhances NET formation in cystic fibrosis","volume":"73","author":"Gray","year":"2018","journal-title":"Thorax"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1211","DOI":"10.1016\/j.intimp.2013.05.035","article-title":"Aberrant neutrophil functions in stable chronic obstructive pulmonary disease: The neutrophil as an immunotherapeutic target","volume":"17","author":"Stockley","year":"2013","journal-title":"Int. Immunopharmacol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"509","DOI":"10.3109\/08820139.2015.1041606","article-title":"Chemotactic and Phagocytic Activity of Blood Neutrophils in Allergic Asthma","volume":"44","author":"Mosca","year":"2015","journal-title":"Immunol. Investig."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1910","DOI":"10.1164\/ajrccm.152.6.8520754","article-title":"Neutrophil-derived long-lived oxidants in cystic fibrosis sputum","volume":"152","author":"Delacourt","year":"1995","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"149","DOI":"10.3109\/02770909609054546","article-title":"Increased Spontaneous Production and Generation of Superoxide Anion by Blood Neutrophils in Patients with Asthma","volume":"33","author":"Teramoto","year":"1996","journal-title":"J. Asthma"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"L997","DOI":"10.1152\/ajplung.00156.2002","article-title":"Distinct cytokine production by lung and blood neutrophils from children with cystic fibrosis","volume":"284","author":"Corvol","year":"2003","journal-title":"Am. J. Physiol. Cell. Mol. Physiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1016\/j.jcf.2012.04.010","article-title":"Increase in interleukin-8 production from circulating neutrophils upon antibiotic therapy in cystic fibrosis patients","volume":"11","author":"Montemurro","year":"2012","journal-title":"J. Cyst. Fibros."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"522","DOI":"10.1183\/09031936.00027409","article-title":"Differential gene expression and cytokine production from neutrophils in asthma phenotypes","volume":"35","author":"Baines","year":"2010","journal-title":"Eur. Respir. J."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1111\/j.1365-2249.1992.tb06953.x","article-title":"Decreased polymorphonuclear leucocyte chemotactic response to leukotriene B4 in cystic fibrosis","volume":"89","author":"Lawrence","year":"1992","journal-title":"Clin. Exp. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"867","DOI":"10.1136\/thx.49.9.867","article-title":"Desensitisation of neutrophil responses by systemic interleukin 8 in cystic fibrosis","volume":"49","author":"Dai","year":"1994","journal-title":"Thorax"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1136\/thorax.56.1.62","article-title":"Directed neutrophil migration to IL-8 is increased in cystic fibrosis: A study of the effect of erythromycin","volume":"56","author":"Brennan","year":"2001","journal-title":"Thorax"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Tissue-based map of the human proteome","volume":"347","author":"Fagerberg","year":"2015","journal-title":"Science"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1189\/jlb.1003479","article-title":"Synergy between proinflammatory ligands of G protein-coupled receptors in neutrophil activation and migration","volume":"76","author":"Gouwy","year":"2004","journal-title":"J. Leukoc. Biol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1002\/eji.201444818","article-title":"Serum amyloid A chemoattracts immature dendritic cells and indirectly provokes monocyte chemotaxis by induction of cooperating CC and CXC chemokines","volume":"45","author":"Gouwy","year":"2015","journal-title":"Eur. J. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"869","DOI":"10.4049\/jimmunol.1700905","article-title":"A High-Throughput Real-Time Imaging Technique to Quantify NETosis and Distinguish Mechanisms of Cell Death in Human Neutrophils","volume":"200","author":"Gupta","year":"2018","journal-title":"J. Immunol."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-017-15027-y","article-title":"Monocytes from patients with Primary Ciliary Dyskinesia show enhanced inflammatory properties and produce higher levels of pro-inflammatory cytokines","volume":"7","author":"Cockx","year":"2017","journal-title":"Sci. Rep."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6172\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:11:56Z","timestamp":1760163116000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6172"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,8]]},"references-count":62,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ijms22126172"],"URL":"https:\/\/doi.org\/10.3390\/ijms22126172","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,8]]}}}