{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,25]],"date-time":"2025-10-25T11:32:36Z","timestamp":1761391956599,"version":"build-2065373602"},"reference-count":46,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T00:00:00Z","timestamp":1623628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006109","name":"Vedeck\u00e1 Grantov\u00e1 Agent\u00fara M\u0160VVa\u0160 SR a SAV","doi-asserted-by":"publisher","award":["1\/0173\/19","1\/0204\/16"],"award-info":[{"award-number":["1\/0173\/19","1\/0204\/16"]}],"id":[{"id":"10.13039\/501100006109","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Breast cancer is the most common cancer in females. The aim of this study was to determine the effect of paclitaxel (PTX) and doxorubicin (DOX) therapy on the \u03b2III-tubulin, carbonic anhydrase IX (CA IX), and survivin expression in chemically-induced rat mammary tumors. Animals with induced mammary carcinogenesis were randomly divided into treatment groups and an untreated group. The total proportion of tumors, the proportion of carcinoma in situ (CIS), and invasive carcinoma (IC) were evaluated. Protein expression in tumor tissue was determined using IHC. Statistical analysis of the data, evaluated by Fisher-exact test and unpaired t-test. Significantly increased levels of proteins in the tumor cells were confirmed using the IHC method for all studied proteins. The expression of \u03b2III-tubulin, CA IX, and survivin increased significantly after treatment with both cytostatics (PTX and DOX). Depending on the type of tumor, a significant increase in all proteins was observed in IC samples after PTX treatment, and CA IX expression after DOX treatment. In CIS samples, a significant increase of \u03b2III-tubulin and survivin expression was observed after a DOX treatment. The results suggest that \u03b2III-tubulin, survivin, and CA IX may be significant drug resistance markers and the clinical regulation of their activity may be an effective means of reversing this resistance.<\/jats:p>","DOI":"10.3390\/ijms22126363","type":"journal-article","created":{"date-parts":[[2021,6,14]],"date-time":"2021-06-14T10:30:25Z","timestamp":1623666625000},"page":"6363","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Influence of Paclitaxel and Doxorubicin Therapy of \u00dfIII-Tubulin, Carbonic Anhydrase IX, and Survivin in Chemically Induced Breast Cancer in Female Rat"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3072-1189","authenticated-orcid":false,"given":"Alena","family":"Pastornick\u00e1","sequence":"first","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Ryb\u00e1rov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sl\u00e1vka","family":"Draho\u0161ov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Pathological Anatomy, Jessenius Faculty of Medicine, Comenius University, Koll\u00e1rova 2, 036 59 Martin, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3629-5088","authenticated-orcid":false,"given":"Jozef","family":"Mihalik","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Krehe\u013eov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andriana","family":"Pavliuk-Karachevtseva","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7888-7719","authenticated-orcid":false,"given":"Ingrid","family":"Hodorov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Medical Faculty, \u0160af\u00e1rik University, \u0160rob\u00e1rova 2, 041 83 Ko\u0161ice, Slovakia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"394","DOI":"10.3322\/caac.21492","article-title":"Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries","volume":"68","author":"Bray","year":"2018","journal-title":"CA Cancer J. 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