{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:08:10Z","timestamp":1760234890576,"version":"build-2065373602"},"reference-count":54,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"S\u00e3o Paulo Research Foundation (FAPESP)","award":["#2018\/05181-7 and #2018\/08631-3"],"award-info":[{"award-number":["#2018\/05181-7 and #2018\/08631-3"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The mechanisms through which maternal immunization can modulate offspring thymic maturation of lymphocytes are not fully understood. Here, we aimed to evaluate whether maternal OVA-immunization can inhibit the maturation of IL-17-producing \u03b3\u03b4T cells in offspring thymus, and if this mechanism has epigenetic implications mediated by microRNAs (miRNAs) expression. Wild-type (WT) C57BL\/6 females were immunized with OVA in Alum or Alum alone and were mated with normal WT males. Evaluating their offspring thymus at 3 or 20 days old (d.o.), we observed that maternal OVA immunization could inhibit the thymic frequency of offspring CD27- and IL-17+ \u03b3\u03b4T cells at the neonatal and until 20 days old. Furthermore, we evaluated the expression of function-related \u03b3 and \u03b4 variable \u03b3\u03b4TCR chains (V\u03b31, V\u03b32, V\u03b33, V\u03b44, and V\u03b46.3), observing that maternal OVA-immunization inhibits V\u03b32 chains expression. The small RNAs (sRNAs), particularly miRNAs, and messenger RNAs (mRNA) expression profiles by pools of thymus tissue samples (from 9 to 11 mice) from offspring OVA-immunized or Alum-immunized mothers were analyzed via Illumina sequencing platform and bioinformatics approaches. Using a fold change &gt;4, our results showed that seven miRNAs (mmu-miR-126a-3p, 101a-3p, 744-3p,142-5p, 15a-5p, 532-5p, and 98-5p) were differentially expressed between both groups. Ten target genes were predicted to interact with the seven selected miRNAs. There were no enriched categories of gene ontology functional annotation and pathway enrichment analysis for the target genes. Interestingly, four of the identified miRNAs (mmu-miR-15a, mmu-miR-101 mmu-miR-126, and mmu-miR-142) are related to IL-17 production. Our data is of significance because we demonstrate that maternal immunization can modulate offspring thymic maturation of IL-17-producing \u03b3\u03b4T cells possibly by an epigenetic mechanism mediated by miRNAs.<\/jats:p>","DOI":"10.3390\/ijms22126633","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T13:29:58Z","timestamp":1624282198000},"page":"6633","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Preconceptional Immunization Can Modulate Offspring Intrathymic IL-17-Producing \u03b3\u03b4T Cells with Epigenetic Implications Mediated by microRNAs"],"prefix":"10.3390","volume":"22","author":[{"given":"Thamires Rodrigues","family":"de-Sousa","sequence":"first","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rodrigo","family":"Pess\u00f4a","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrezza","family":"Nascimento","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz Oliveira","family":"Fagundes","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F\u00e1bio da Ressurei\u00e7\u00e3o","family":"Sgnotto","sequence":"additional","affiliation":[{"name":"Division of Hematology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 01246-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto Jos\u00e9 da Silva","family":"Duarte","sequence":"additional","affiliation":[{"name":"Division of Pathology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sabri Saeed","family":"Sanabani","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6092-8394","authenticated-orcid":false,"given":"Jefferson Russo","family":"Victor","sequence":"additional","affiliation":[{"name":"Laboratory of Medical Investigation LIM-56, Division of Clinical Dermatology, Medical School, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, Brazil"},{"name":"Faculdades Metropolitanas Unidas (FMU), School of Health Sciences, S\u00e3o Paulo 04505-002, Brazil"},{"name":"Medical School, Santo Amaro University (UNISA), S\u00e3o Paulo 04829-300, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e541","DOI":"10.1111\/j.1365-2567.2008.03028.x","article-title":"Balance between early life tolerance and sensitization in allergy: Dependence on the timing and intensity of prenatal and postnatal allergen exposure of the mother","volume":"128","author":"Fusaro","year":"2009","journal-title":"Immunology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1282","DOI":"10.4049\/jimmunol.179.2.1282","article-title":"Maternal transmission of resistance to development of allergic airway disease","volume":"179","author":"Matson","year":"2007","journal-title":"J. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"170","DOI":"10.1038\/nm1718","article-title":"Breast milk-mediated transfer of an antigen induces tolerance and protection from allergic asthma","volume":"14","author":"Verhasselt","year":"2008","journal-title":"Nat. Med."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Ellertsen, L.K., Nygaard, U.C., Melkild, I., and L\u00f8vik, M. (2010). Maternal allergen immunisation to prevent sensitisation in offspring: Th2-polarising adjuvants are more efficient than a Th1-polarising adjuvant in mice. BMC Immunol., 11.","DOI":"10.1186\/1471-2172-11-8"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Victor, J.R., Muniz, B.P., Fusaro, A.E., de Brito, C.A., Taniguchi, E.F., Duarte, A.J.S., and Sato, M.N. (2010). Maternal immunization with ovalbumin prevents neonatal allergy development and up-regulates inhibitory receptor Fc gamma RIIB expression on B cells. BMC Immunol., 11.","DOI":"10.1186\/1471-2172-11-11"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"De Lima Lira, A.A., de Oliveira, M.G., de Oliveira, L.M., da Silva Duarte, A.J., Sato, M.N., and Victor, J.R. (2014). Maternal immunization with ovalbumin or Dermatophagoides pteronyssinus has opposing effects on Fc gamma RIIb expression on offspring B cells. Allergy Asthma Clin. Immunol., 10.","DOI":"10.1186\/1710-1492-10-47"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"208","DOI":"10.1159\/000053865","article-title":"Influence of maternal murine immunization with Dermatophagoides pteronyssinus extract on the type I hypersensitivity response in offspring","volume":"127","author":"Fusaro","year":"2002","journal-title":"Int. Arch. Allergy Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"3485","DOI":"10.4049\/jimmunol.171.7.3485","article-title":"Critical role of preconceptional immunization for protective and nonpathological specific immunity in murine neonates","volume":"171","author":"Uthoff","year":"2003","journal-title":"J. Immunol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1067\/mai.2003.39","article-title":"Preconception maternal immunization to dust mite inhibits the type I hypersensitivity response of offspring","volume":"111","author":"Victor","year":"2003","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_10","first-page":"10","article-title":"Preconception immunization can modulate intracellular Th2 cytokine profile in offspring: In vivo influence of interleukin 10 and B\/T cell collaboration","volume":"43","author":"Russo","year":"2018","journal-title":"Cent. Eur. J. Immunol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1111\/j.1365-2567.2007.02618.x","article-title":"Maternal-fetal interaction: Preconception immunization in mice prevents neonatal sensitization induced by allergen exposure during pregnancy and breastfeeding","volume":"122","author":"Fusaro","year":"2007","journal-title":"Immunology"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Garcia de Oliveira, M., da Ressurei\u00e7\u00e3o Sgnotto, F., Rodrigues de Sousa, T., Fagundes, B.O., Duarte, A.J.D.S., and Victor, J.R. (2020). Preconceptional immunization with an allergen inhibits offspring thymic Th17 cells maturation without influence on Th1 and Th2 cells. Eur. Cytokine Netw.","DOI":"10.1684\/ecn.2020.0452"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2371","DOI":"10.1007\/s00018-011-0700-z","article-title":"Regulation and function of IL-17A- and IL-22-producing \u03b3\u03b4 T cells","volume":"68","author":"Morita","year":"2011","journal-title":"Cell Mol. Life Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Victor, J.R., Lezmi, G., and Leite-de-Moraes, M. (2020). New Insights into Asthma Inflammation: Focus on iNKT, MAIT, and \u03b3\u03b4T Cells. Clin. Rev. Allergy Immunol.","DOI":"10.1007\/s12016-020-08784-8"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"de Sousa, T.R., and Victor, J.R. (2020). Natural Self-Ligand Gamma Delta T Cell Receptors (\u03b3\u03b4TCRs) Insight: The Potential of Induced IgG. Vaccines, 8.","DOI":"10.3390\/vaccines8030436"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1111\/all.13618","article-title":"\u03b3\u03b4 T cells amplify Blomia tropicalis-induced allergic airway disease","volume":"74","author":"Belkadi","year":"2019","journal-title":"Allergy"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"de Oliveira, M.G., de Lima Lira, A.A., da Ressurei\u00e7\u00e3o Sgnotto, F., Inoue, A.H.S., Santos, L.S., Nakamatsu, B.Y., Duarte, A.J.D.S., Leite-de-Moraes, M., and Victor, J.R. (2019). Maternal IgG impairs the maturation of offspring intrathymic IL-17-producing \u03b3\u03b4T cells: Implications for murine and human allergies. Clin. Exp. Allergy.","DOI":"10.1111\/cea.13393"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1080\/21645515.2016.1244592","article-title":"Allergen-specific IgG as a mediator of allergy inhibition: Lessons from mother to child","volume":"13","author":"Victor","year":"2017","journal-title":"Hum. Vaccines Immunother."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Victor, J.R. (2020). Do different IgG repertoires play a role in B- and T-cell functional modulation during ontogeny? The \u201chooks without bait\u201d theory. Immunol. Cell Biol.","DOI":"10.1111\/imcb.12335"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"780386","DOI":"10.1155\/2014\/780386","article-title":"Influence of maternal immunization with allergens on the thymic maturation of lymphocytes with regulatory potential in children: A broad field for further exploration","volume":"2014","author":"Victor","year":"2014","journal-title":"J. Immunol. Res."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Inoue, A.H.S., Lira, A.A.L., de-Oliveira, M.G., de Sousa, T.R., Sgnotto, F.D.R., Duarte, A.J.D.S., and Victor, J.R. (2020). The Potential of IgG to Induce Murine and Human Thymic Maturation of IL-10+ B Cells (B10) Revealed in a Pilot Study. Cells, 9.","DOI":"10.3390\/cells9102239"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Santos, L.S., Sgnotto, F.D.R., Sousa, T.R., Orfali, R.L., Aoki, V., Duarte, A.J.D.S., and Victor, J.R. (2019). IgG from atopic dermatitis patients induces non-atopic infant thymic invariant natural killer T (iNKT) cells to produce IL-4, IL-17, and IL-10. Int. J. Derm.","DOI":"10.1111\/ijd.14688"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"e56","DOI":"10.1097\/QAI.0000000000002182","article-title":"IgG From HIV-1-Exposed Seronegative and HIV-1-Infected Subjects Differently Modulates IFN-\u03b3 Production by Thymic T and B Cells","volume":"82","year":"2019","journal-title":"J. Acquir. Immune Defic. Syndr."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Santos, L.S., Sgnotto, F.D.R., Inoue, A.H.S., Padreca, A.F., Menghini, R.P., Duarte, A.J.D.S., and Victor, J.R. (2019). IgG from Non-atopic Individuals Induces In Vitro IFN-\u03b3 and IL-10 Production by Human Intra-thymic \u03b3\u03b4T Cells: A Comparison with Atopic IgG and IVIg. Arch. Immunol. Ther. Exp.","DOI":"10.1007\/s00005-019-00545-6"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1111\/ijd.13907","article-title":"IgG from atopic dermatitis patients induces IL-17 and IL-10 production in infant intrathymic TCD4 and TCD8 cells","volume":"57","author":"Sgnotto","year":"2018","journal-title":"Int. J. Derm."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"de Oliveira, M.G., Lira, A.A.L., Sgnotto, F.D.R., Inoue, A.H.S., Beltrame, G.R., da Silva, D., Menghini, R.P., Duarte, A.J.D.S., and Victor, J.R. (2018). Maternal immunization downregulates offspring TCD4 regulatory cells (Tregs) thymic maturation without implications for allergy inhibition. Scand. J. Immunol., e12721.","DOI":"10.1111\/sji.12721"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1186\/s13223-017-0195-8","article-title":"Preconception allergen sensitization can induce B10 cells in offspring: A potential main role for maternal IgG","volume":"13","author":"Oliveira","year":"2017","journal-title":"Allergy Asthma Clin. Immunol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1563","DOI":"10.1080\/21645515.2017.1299299","article-title":"Low doses of IgG from atopic individuals can modulate in vitro IFN-\u03b3 production by human intra-thymic TCD4 and TCD8 cells: An IVIg comparative approach","volume":"13","author":"Sgnotto","year":"2017","journal-title":"Hum. Vaccin. Immunother."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1038\/ni.1717","article-title":"CD27 is a thymic determinant of the balance between interferon-gamma- and interleukin 17-producing gammadelta T cell subsets","volume":"10","author":"Ribot","year":"2009","journal-title":"Nat. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1007\/978-1-4614-6217-0_11","article-title":"Differentiation and activation of \u03b3\u03b4 T Lymphocytes: Focus on CD27 and CD28 costimulatory receptors","volume":"785","author":"Ribot","year":"2013","journal-title":"Adv. Exp. Med. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1006\/smim.1996.0046","article-title":"Origin and development of the gamma delta T-cell system in sheep: A critical role for the thymus in the generation of TcR diversity and tissue tropism","volume":"8","author":"Cahill","year":"1996","journal-title":"Semin. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1225","DOI":"10.1084\/jem.172.4.1225","article-title":"Late dominance of the inflammatory process in murine influenza by gamma\/delta + T cells","volume":"172","author":"Carding","year":"1990","journal-title":"J. Exp. Med."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1038\/nri797","article-title":"Gammadelta T cells: Functional plasticity and heterogeneity","volume":"2","author":"Carding","year":"2002","journal-title":"Nat. Rev. Immunol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.smim.2010.03.006","article-title":"gammadelta T cell subsets: A link between TCR and function?","volume":"22","author":"Born","year":"2010","journal-title":"Semin. Immunol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"467","DOI":"10.1038\/nri2781","article-title":"Gammadelta T cell effector functions: A blend of innate programming and acquired plasticity","volume":"10","author":"Bonneville","year":"2010","journal-title":"Nat. Rev. Immunol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1038\/ni.2247","article-title":"Intrathymic programming of effector fates in three molecularly distinct \u03b3\u03b4 T cell subtypes","volume":"13","author":"Narayan","year":"2012","journal-title":"Nat. Immunol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"5576","DOI":"10.4049\/jimmunol.179.8.5576","article-title":"Exacerbation of collagen-induced arthritis by oligoclonal, IL-17-producing gamma delta T cells","volume":"179","author":"Roark","year":"2007","journal-title":"J. Immunol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3076","DOI":"10.4049\/jimmunol.163.6.3076","article-title":"IL-4-producing gamma delta T cells that express a very restricted TCR repertoire are preferentially localized in liver and spleen","volume":"163","author":"Gerber","year":"1999","journal-title":"J. Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1016\/0092-8674(93)90529-Y","article-title":"The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14","volume":"75","author":"Lee","year":"1993","journal-title":"Cell"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1016\/j.cell.2009.01.045","article-title":"Small RNAs as guardians of the genome","volume":"136","author":"Malone","year":"2009","journal-title":"Cell"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Haas, J.D., Nistala, K., Petermann, F., Saran, N., Chennupati, V., Schmitz, S., Korn, T., Wedderburn, L.R., Forster, R., and Krueger, A. (2011). Expression of miRNAs miR-133b and miR-206 in the Il17a\/f locus is co-regulated with IL-17 production in alphabeta and gammadelta T cells. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0020171"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Schmolka, N., Papotto, P.H., Romero, P.V., Amado, T., Enguita, F.J., Amorim, A., Rodrigues, A.F., Gordon, K.E., Coroadinha, A.S., and Boldin, M. (2018). MicroRNA-146a controls functional plasticity in gammadelta T cells by targeting NOD1. Sci. Immunol., 3.","DOI":"10.1126\/sciimmunol.aao1392"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.aller.2018.04.001","article-title":"Preconceptional allergen immunization can induce offspring IL-17 secreting B cells (B17): Do they share similarities with regulatory B10 cells?","volume":"46","author":"Lira","year":"2018","journal-title":"Allergol. Immunopathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.immuni.2004.06.008","article-title":"Positive selection of dendritic epidermal gammadelta T cell precursors in the fetal thymus determines expression of skin-homing receptors","volume":"21","author":"Xiong","year":"2004","journal-title":"Immunity"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"92","DOI":"10.1101\/gr.082701.108","article-title":"Most mammalian mRNAs are conserved targets of microRNAs","volume":"19","author":"Friedman","year":"2009","journal-title":"Genome Res."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"2519","DOI":"10.1084\/jem.20061692","article-title":"A role for Dicer in immune regulation","volume":"203","author":"Cobb","year":"2006","journal-title":"J. Exp. Med."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1142","DOI":"10.1002\/eji.201746987","article-title":"MicroRNA-142 controls thymocyte proliferation","volume":"47","author":"Mildner","year":"2017","journal-title":"Eur. J. Immunol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1002\/hep.29573","article-title":"MicroRNA 15a\/16-1 suppresses aryl hydrocarbon receptor-dependent interleukin-22 secretion in CD4","volume":"67","author":"Lu","year":"2018","journal-title":"Hepatology"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2287","DOI":"10.1007\/s10753-020-01298-7","article-title":"Influences of Regulation of miR-126 on Inflammation, Th17\/Treg Subpopulation Differentiation, and Lymphocyte Apoptosis through Caspase Signaling Pathway in Sepsis","volume":"43","author":"Zou","year":"2020","journal-title":"Inflammation"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"277","DOI":"10.1139\/bcb-2019-0174","article-title":"MiR-126 targets IL-17A to enhance proliferation and inhibit apoptosis in high-glucose-induced human retinal endothelial cells","volume":"98","author":"Chen","year":"2020","journal-title":"Biochem. Cell Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1007\/s10753-020-01325-7","article-title":"MicroRNA-532-3p Regulates Pro-Inflammatory Human THP-1 Macrophages by Targeting ASK1\/p38 MAPK Pathway","volume":"44","author":"Dinesh","year":"2021","journal-title":"Inflammation"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2311","DOI":"10.3892\/ol.2020.11803","article-title":"Small RNA profiles of HTLV-1 asymptomatic carriers with monoclonal and polyclonal rearrangement of the T-cell antigen receptor \u03b3-chain using massively parallel sequencing: A pilot study","volume":"20","author":"Nascimento","year":"2020","journal-title":"Oncol. Lett."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1186\/s13027-020-00343-2","article-title":"Global expression of noncoding RNome reveals dysregulation of small RNAs in patients with HTLV-1-associated adult T-cell leukemia: A pilot study","volume":"16","author":"Nascimento","year":"2021","journal-title":"Infect. Agent Cancer"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Sticht, C., De La Torre, C., Parveen, A., and Gretz, N. (2018). miRWalk: An online resource for prediction of microRNA binding sites. PLoS ONE, 13.","DOI":"10.1371\/journal.pone.0206239"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6633\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:20:22Z","timestamp":1760163622000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6633"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,21]]},"references-count":54,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ijms22126633"],"URL":"https:\/\/doi.org\/10.3390\/ijms22126633","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2021,6,21]]}}}