{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:11:38Z","timestamp":1783487498575,"version":"3.55.0"},"reference-count":52,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T00:00:00Z","timestamp":1624233600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Swine Innovation Team in Guangdong Modern Agricultural Research System","award":["2020KJ126"],"award-info":[{"award-number":["2020KJ126"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31802033"],"award-info":[{"award-number":["31802033"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007162","name":"Department of Science and Technology of Guangdong Province","doi-asserted-by":"publisher","award":["2019A1515011835"],"award-info":[{"award-number":["2019A1515011835"]}],"id":[{"id":"10.13039\/501100007162","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Promotion Project on Preservation and UtiIization of Local Breed of Live-stock and Poultry","award":["4300-F18260"],"award-info":[{"award-number":["4300-F18260"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Due to the high rate of spontaneous abortion (SAB) in porcine pregnancy, there is a major interest and concern on commercial pig farming worldwide. Whereas the perturbed immune response at the maternal\u2013fetal interface is an important mechanism associated with the spontaneous embryo loss in the early stages of implantation in porcine, data on the specific regulatory mechanism of the SAB at the end stage of the implantation remains scant. Therefore, we used high-throughput sequencing and bioinformatics tools to analyze the healthy and arresting endometrium on day 28 of pregnancy. We identified 639 differentially expressed lncRNAs (DELs) and 2357 differentially expressed genes (DEGs) at the end stage of implantation, and qRT-PCR was used to verify the sequencing data. Gene set variation analysis (GSVA), gene set enrichment analysis (GSEA), and immunohistochemistry analysis demonstrated weaker immune response activities in the arresting endometrium compared to the healthy one. Using the lasso regression analysis, we screened the DELs and constructed an immunological competitive endogenous RNA (ceRNA) network related to SAB, including 4 lncRNAs, 11 miRNAs, and 13 genes. In addition, Blast analysis showed the applicability of the constructed ceRNA network in different species, and subsequently determined HOXA-AS2 in pigs. Our study, for the first time, demonstrated that the SAB events at the end stages of implantation is associated with the regulation of immunobiological processes, and a specific molecular regulatory network was obtained. These novel findings may provide new insight into the possibility of increasing the litter size of sows, making pig breeding better and thus improving the efficiency of animal husbandry production.<\/jats:p>","DOI":"10.3390\/ijms22126644","type":"journal-article","created":{"date-parts":[[2021,6,21]],"date-time":"2021-06-21T13:29:58Z","timestamp":1624282198000},"page":"6644","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Integrated Insight into the Molecular Mechanisms of Spontaneous Abortion during Early Pregnancy in Pigs"],"prefix":"10.3390","volume":"22","author":[{"given":"Xupeng","family":"Zang","sequence":"first","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8732-7377","authenticated-orcid":false,"given":"Ting","family":"Gu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenjing","family":"Wang","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chen","family":"Zhou","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Ding","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengchen","family":"Gu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiqian","family":"Xu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanshe","family":"Xie","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zicong","family":"Li","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"},{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gengyuan","family":"Cai","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9927-2414","authenticated-orcid":false,"given":"Bin","family":"Hu","sequence":"additional","affiliation":[{"name":"Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6554-7918","authenticated-orcid":false,"given":"Linjun","family":"Hong","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenfang","family":"Wu","sequence":"additional","affiliation":[{"name":"National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Guangdong Provincial Key Laboratory of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China"},{"name":"Lingnan Guangdong Laboratory of Modern Agriculture, Guangzhou 510642, China"},{"name":"State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1152\/physiolgenomics.00022.2008","article-title":"Identification of differential gene expression during porcine conceptus rapid trophoblastic elongation and attachment to uterine luminal epithelium","volume":"36","author":"Ross","year":"2009","journal-title":"Physiol. Genom."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/S0169-409X(99)00003-4","article-title":"Comparative placental structure","volume":"38","author":"Enders","year":"1999","journal-title":"Adv. Drug Deliv. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/2049-1891-5-51","article-title":"Cytokines from the pig conceptus: Roles in conceptus development in pigs","volume":"5","author":"Geisert","year":"2014","journal-title":"J. Anim. Sci. Biotechnol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1146\/annurev-immunol-032713-120145","article-title":"Chemokines and Chemokine Receptors: Positioning Cells for Host Defense and Immunity","volume":"32","author":"Griffith","year":"2014","journal-title":"Annu. Rev. Immunol."},{"key":"ref_5","unstructured":"Bidarimath, M. (2016). Immune-Angiogenesis Mechanisms Associated with Porcine Pregnancy Success and Failure. [Ph.D. Thesis, Queen\u2019s University]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"1875","DOI":"10.1095\/biolreprod66.6.1875","article-title":"Conceptus influences the distribution of uterine leukocytes during early porcine pregnancy","volume":"66","author":"Engelhardt","year":"2002","journal-title":"Biol. Reprod."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1053\/plac.1998.0350","article-title":"Immunohistochemical Localization of Vascular Endothelial Growth Factor (VEGF) and its Two Specific Receptors, Flt-1 and KDR, in the Porcine Placenta and Non-pregnant Uterus","volume":"20","author":"Winther","year":"1999","journal-title":"Placenta"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"877","DOI":"10.1189\/jlb.0507330","article-title":"Lymphocyte contributions to altered endometrial angiogenesis during early and midgestation fetal loss","volume":"82","author":"Tayade","year":"2007","journal-title":"J. Leukoc. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"584","DOI":"10.1038\/nri1897","article-title":"Immunology of placentation in eutherian mammals","volume":"6","author":"Moffett","year":"2006","journal-title":"Nat. Rev. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1262\/jrd.18170","article-title":"A review of gene expression in porcine endometrial lymphocytes, endothelium and trophoblast during pregnancy success and failure","volume":"53","author":"Tayade","year":"2007","journal-title":"J. Reprod. Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1161\/CIRCRESAHA.117.312512","article-title":"RNA editing: Unexplored opportunities in the cardiovascular system","volume":"122","author":"Uchida","year":"2018","journal-title":"Circul. Res."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Zimmer-Bensch, G. (2019). Emerging Roles of Long Non-Coding RNAs as Drivers of Brain Evolution. Cells, 8.","DOI":"10.20944\/preprints201911.0031.v1"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"56383","DOI":"10.18632\/oncotarget.10891","article-title":"Construction and analysis of dysregulated lncRNA-associated ceRNA network identified novel lncRNA biomarkers for early diagnosis of human pancreatic cancer","volume":"7","author":"Zhou","year":"2016","journal-title":"Oncotarget"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"656","DOI":"10.1101\/gad.320523.118","article-title":"The long noncoding RNA Falcor regulates Foxa2 expression to maintain lung epithelial homeostasis and promote regeneration","volume":"33","author":"Swarr","year":"2019","journal-title":"Genes Dev."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"875","DOI":"10.1080\/15476286.2020.1827591","article-title":"Long noncoding RNA lnc-NAP sponges mmu-miR-139-5p to modulate Nanog functions in mouse ESCs and embryos","volume":"18","author":"Xie","year":"2020","journal-title":"RNA Biol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"12945","DOI":"10.1111\/jcmm.15889","article-title":"Hyperbaric oxygen-induced long non-coding RNA MALAT1 exosomes suppress MicroRNA-92a expression in a rat model of acute myocardial infarction","volume":"24","author":"Shyu","year":"2020","journal-title":"J. Cell. Mol. Med."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.anireprosci.2017.01.001","article-title":"Adiponectin, orexin A and orexin B concentrations in the serum and uterine luminal fluid during early pregnancy of pigs","volume":"178","author":"Smolinska","year":"2017","journal-title":"Anim. Reprod. Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"471","DOI":"10.1016\/0008-8749(88)90199-2","article-title":"Normal murine and porcine embryos recruit NK cells to the uterus","volume":"115","author":"Croy","year":"1988","journal-title":"Cell. Immunol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"959","DOI":"10.1261\/rna.044560.114","article-title":"The RIDL hypothesis: Transposable elements as functional domains of long noncoding RNAs","volume":"20","author":"Johnson","year":"2014","journal-title":"RNA"},{"key":"ref_20","first-page":"769","article-title":"Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs","volume":"433","author":"Lim","year":"2005","journal-title":"Nat. Cell Biol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"762","DOI":"10.2527\/jas1987.653762x","article-title":"Relationships Between Uterine Length and Number of Fetuses and Prenatal Mortality in Pigs","volume":"65","author":"Wu","year":"1987","journal-title":"J. Anim. Sci."},{"key":"ref_22","first-page":"247","article-title":"Global Transcriptomic Analyses Reveal Genes Involved in Conceptus Development During the Implantation Stages in Pigs","volume":"12","author":"Zang","year":"2021","journal-title":"Front. Genet."},{"key":"ref_23","first-page":"177","article-title":"Initial vascularisation in the pig placenta: I. Demonstration of nonglandular areas by histology and corrosion casts","volume":"238","author":"Dantzer","year":"1994","journal-title":"Anat. Rec. Adv. Integr. Anat. Evol. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"148","DOI":"10.4049\/jimmunol.176.1.148","article-title":"Differential Gene Expression in Endometrium, Endometrial Lymphocytes, and Trophoblasts during Successful and Abortive Embryo Implantation","volume":"176","author":"Tayade","year":"2005","journal-title":"J. Immunol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"588","DOI":"10.1002\/jez.1402660609","article-title":"Comparative placentation in ungulates","volume":"266","author":"King","year":"1993","journal-title":"J. Exp. Zool."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"759","DOI":"10.1006\/cbir.1994.1105","article-title":"Adhesion molecule expression and infiltrating maternal leucocyte phenotypes during blastocyst implantation in the pig","volume":"18","author":"Whyte","year":"1994","journal-title":"Cell Biol. Int."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"155","DOI":"10.1161\/CIRCRESAHA.117.311802","article-title":"Long noncoding RNA discovery in cardiovascular disease: Decoding form to function","volume":"122","author":"Sallam","year":"2018","journal-title":"Circul. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.devcel.2012.12.012","article-title":"The Tissue-Specific lncRNA Fendrr Is an Essential Regulator of Heart and Body Wall Development in the Mouse","volume":"24","author":"Grote","year":"2013","journal-title":"Dev. Cell"},{"key":"ref_29","first-page":"871","article-title":"Long non-coding RNA ADNCR suppresses adipogenic differentiation by targeting miR-204","volume":"1859","author":"Li","year":"2016","journal-title":"Biochim. Biophys. Acta (BBA)-Bioenergy"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"17378","DOI":"10.1002\/jcb.29002","article-title":"Aberrant expression of imprinted lncRNA MEG8 causes trophoblast dysfunction and abortion","volume":"120","author":"Sheng","year":"2019","journal-title":"J. Cell. Biochem."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1775","DOI":"10.1101\/gr.132159.111","article-title":"The GENCODE v7 catalog of human long noncoding RNAs: Analysis of their gene structure, evolution, and expression","volume":"22","author":"Derrien","year":"2012","journal-title":"Genome Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1433","DOI":"10.1101\/gr.078378.108","article-title":"Long noncoding RNAs in mouse embryonic stem cell pluripotency and differentiation","volume":"18","author":"Dinger","year":"2008","journal-title":"Genome Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"2495","DOI":"10.1172\/JCI83361","article-title":"MicroRNA-140-5p and SMURF1 regulate pulmonary arterial hypertension","volume":"126","author":"Rothman","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3697","DOI":"10.1242\/dev.110833","article-title":"Multiple roles for HOXA3 in regulating thymus and parathyroid differentiation and morphogenesis in mouse","volume":"141","author":"Chojnowski","year":"2014","journal-title":"Development"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.ydbio.2016.05.010","article-title":"Temporal and spatial requirements for Hoxa3 in mouse embryonic development","volume":"415","author":"Chojnowski","year":"2016","journal-title":"Dev. Biol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1640","DOI":"10.1016\/j.biopha.2018.10.046","article-title":"TCF7L2 activated HOXA-AS2 decreased the glucocorticoid sensitivity in acute lymphoblastic leukemia through regulating HOXA3\/EGFR\/Ras\/Raf\/MEK\/ERK pathway","volume":"109","author":"Zhao","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1089\/hum.2018.109","article-title":"LncRNA HOXA-AS2 Facilitates Tumorigenesis and Progression of Papillary Thyroid Cancer by Modulating the miR-15a-5p\/HOXA3 Axis","volume":"30","author":"Jiang","year":"2019","journal-title":"Hum. Gene Ther."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e123","DOI":"10.1093\/nar\/gkp596","article-title":"Transcriptome analysis by strand-specific sequencing of complementary DNA","volume":"37","author":"Parkhomchuk","year":"2009","journal-title":"Nucleic Acids Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1038\/nbt.3122","article-title":"StringTie enables improved reconstruction of a transcriptome from RNA-seq reads","volume":"33","author":"Pertea","year":"2015","journal-title":"Nat. Biotechnol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"e166","DOI":"10.1093\/nar\/gkt646","article-title":"Utilizing sequence intrinsic composition to classify protein-coding and long non-coding transcripts","volume":"41","author":"Sun","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"D222","DOI":"10.1093\/nar\/gkt1223","article-title":"Pfam: The protein families database","volume":"42","author":"Finn","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"W12","DOI":"10.1093\/nar\/gkx428","article-title":"CPC2: A fast and accurate coding potential calculator based on sequence intrinsic features","volume":"45","author":"Kang","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_44","first-page":"4.10.1\u20134.10.14","article-title":"Using RepeatMasker to Identify Repetitive Elements in Genomic Sequences","volume":"25","author":"Chen","year":"2009","journal-title":"Curr. Protoc. Bioinform."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"77","DOI":"10.3102\/10769986027001077","article-title":"Quick and Easy Implementation of the Benjamini-Hochberg Procedure for Controlling the False Positive Rate in Multiple Comparisons","volume":"27","author":"Thissen","year":"2002","journal-title":"J. Educ. Behav. Stat."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"D1034","DOI":"10.1093\/nar\/gky905","article-title":"LncRNADisease 2.0: An updated database of long non-coding RNA-associated diseases","volume":"47","author":"Bao","year":"2019","journal-title":"Nucleic Acids Res."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1089\/omi.2011.0118","article-title":"Clusterprofiler: An R Package for Comparing Biological Themes Among Gene Clusters","volume":"16","author":"Yu","year":"2012","journal-title":"OMICS: A J. Integr. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/0165-2427(94)90151-1","article-title":"Analyses of monoclonal antibodies reactive with porcine CD44 and CD45","volume":"43","author":"Zuckermann","year":"1994","journal-title":"Veter-Immunol. Immunopathol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1095\/biolreprod.113.114181","article-title":"Expression of Heparanase Is Associated with Breed-Specific Morphological Characters of Placental Folded Bilayer Between Yorkshire and Meishan Pigs1","volume":"90","author":"Hong","year":"2014","journal-title":"Biol. Reprod."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"R1","DOI":"10.1186\/gb-2003-5-1-r1","article-title":"MicroRNA targets in Drosophila","volume":"5","author":"Enright","year":"2003","journal-title":"Genome Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"e05005","DOI":"10.7554\/eLife.05005","article-title":"Predicting effective microRNA target sites in mammalian mRNAs","volume":"4","author":"Agarwal","year":"2015","journal-title":"eLife"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"W451","DOI":"10.1093\/nar\/gkl243","article-title":"RNAhybrid: MicroRNA target prediction easy, fast and flexible","volume":"34","author":"Rehmsmeier","year":"2006","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6644\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:20:24Z","timestamp":1760163624000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/12\/6644"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,21]]},"references-count":52,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2021,6]]}},"alternative-id":["ijms22126644"],"URL":"https:\/\/doi.org\/10.3390\/ijms22126644","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,21]]}}}