{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:14:45Z","timestamp":1791000885725,"version":"4.1.0"},"reference-count":32,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"This research was funded by EUREKA Network project E!13638 Innova-Fertility \u201cAdvanced technologies for innovative infertility treatment\u201d","award":["01.2.2-MITA-K-702-12-0004"],"award-info":[{"award-number":["01.2.2-MITA-K-702-12-0004"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>When looking for the causes and treatments of infertility, much attention is paid to one of the reproductive tissues\u2014the endometrium. Therefore, endometrial stem cells are an attractive target for infertility studies in women of unexplained origin. Menstrual blood stem cells (MenSCs) are morphologically and functionally similar to cells derived directly from the endometrium; with dual expression of mesenchymal and embryonic cell markers, they proliferate and regenerate better than bone marrow mesenchymal stem cells. In addition, menstrual blood stem cells are extracted in a non-invasive and painless manner. In our study, we analyzed the characteristics and the potential for decidualization of menstrual blood stem cells isolated from healthy volunteers and women diagnosed with infertility. We demonstrated that MenSCs express CD44, CD166, CD16, CD15, BMSC, CD56, CD13 and HLA-ABC surface markers, have proliferative properties, and after induction of menstrual stem cell differentiation into epithelial direction, expression of genes related to decidualization (PRL, ESR, IGFBP and FOXO1) and angiogenesis (HIF1, VEGFR2 and VEGFR3) increased. Additionally, the p53, p21, H3K27me3 and HyperAcH4 proteins\u2019 expression increased during MenSCs decidualization, they secrete proteins that are involved in the regulation of the actin cytoskeleton, estrogen and relaxin signaling pathways and the management of inflammatory processes. Our findings reveal the potential use of MenSCs for the treatment of reproductive disorders.<\/jats:p>","DOI":"10.3390\/ijms22136774","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T04:24:36Z","timestamp":1624508676000},"page":"6774","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Menstrual Blood-Derived Endometrial Stem Cells\u2019 Impact for the Treatment Perspective of Female Infertility"],"prefix":"10.3390","volume":"22","author":[{"given":"Giedr\u0117","family":"Skliut\u0117","sequence":"first","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raminta","family":"Bau\u0161yt\u0117","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"},{"name":"Centre of Obstetrics and Gynaecology of the Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Santari\u0161ki\u0173 St., LT-08661 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronika","family":"Borutinskait\u0117","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giedr\u0117","family":"Valiulien\u0117","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Algirdas","family":"Kaupinis","sequence":"additional","affiliation":[{"name":"Proteomic Center, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8478-8517","authenticated-orcid":false,"given":"Mindaugas","family":"Valius","sequence":"additional","affiliation":[{"name":"Proteomic Center, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Rama\u0161auskait\u0117","sequence":"additional","affiliation":[{"name":"Centre of Obstetrics and Gynaecology of the Institute of Clinical Medicine, Faculty of Medicine, Vilnius University, Santari\u0161ki\u0173 St., LT-08661 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1077-9439","authenticated-orcid":false,"given":"R\u016bta","family":"Navakauskien\u0117","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Institute of Biochemistry, Life Sciences Center, Vilnius University, Saul\u0117tekio av. 7, LT-10257 Vilnius, Lithuania"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1016\/j.clinbiochem.2018.03.012","article-title":"Fertility and infertility: Definition and epidemiology","volume":"62","author":"Vander","year":"2018","journal-title":"Clin. 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