{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T03:31:07Z","timestamp":1788838267386,"version":"build-2803163510"},"reference-count":69,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The aim of this study was to investigate the effect of the application of homocysteine as well as its effect under the condition of aerobic physical activity on the activities of matrix metalloproteinases (MMP), lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) in cardiac tissue and on hepato-renal biochemical parameters in sera of rats. Male Wistar albino rats were divided into four groups (n = 10, per group): C: 0.9% NaCl 0.2 mL\/day subcutaneous injection (s.c.); H: homocysteine 0.45 \u00b5mol\/g b.w.\/day s.c.; CPA saline (0.9% NaCl 0.2 mL\/day s.c.) and a program of physical activity on a treadmill; and HPA homocysteine (0.45 \u00b5mol\/g b.w.\/day s.c.) and a program of physical activity on a treadmill. Subcutaneous injection of substances was applied 2 times a day at intervals of 8 h during the first two weeks of experimental protocol. Hcy level in serum was significantly higher in the HPA group compared to the CPA group (p &lt; 0.05). Levels of glucose, proteins, albumin, and hepatorenal biomarkers were higher in active groups compared with the sedentary group. It was demonstrated that the increased activities of LDH (mainly caused by higher activity of isoform LDH2) and mMDH were found under the condition of homocysteine-treated rats plus aerobic physical activity. Independent application of homocysteine did not lead to these changes. Physical activity leads to activation of MMP-2 isoform and to increased activity of MMP-9 isoform in both homocysteine-treated and control rats.<\/jats:p>","DOI":"10.3390\/ijms22136792","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T11:01:38Z","timestamp":1624532498000},"page":"6792","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Four Weeks of Aerobic Training Affects Cardiac Tissue Matrix Metalloproteinase, Lactate Dehydrogenase and Malate Dehydrogenase Enzymes Activities, and Hepatorenal Biomarkers in Experimental Hyperhomocysteinemia in Rats"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8270-3089","authenticated-orcid":false,"given":"Dusan","family":"Todorovic","sequence":"first","affiliation":[{"name":"Faculty of Medicine, Institute of Medical Physiology \u201cRichard Burian\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Stojanovic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Medical Physiology \u201cRichard Burian\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9919-2281","authenticated-orcid":false,"given":"Ana","family":"Medic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Chemistry in Medicine \u201cProf. Dr. Petar Matavulj\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4045-6734","authenticated-orcid":false,"given":"Kristina","family":"Gopcevic","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Chemistry in Medicine \u201cProf. Dr. Petar Matavulj\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2886-7058","authenticated-orcid":false,"given":"Slavica","family":"Mutavdzin","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Medical Physiology \u201cRichard Burian\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanja","family":"Stankovic","sequence":"additional","affiliation":[{"name":"Centre of Medical Biochemistry, Clinical Centre of Serbia, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2196-9438","authenticated-orcid":false,"given":"Dragan","family":"Djuric","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, Institute of Medical Physiology \u201cRichard Burian\u201d, University of Belgrade, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"462","DOI":"10.4330\/wjc.v6.i6.462","article-title":"Antioxidants, inflammation and cardiovascular disease","volume":"6","author":"Mangge","year":"2014","journal-title":"World J. Cardiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1137","DOI":"10.7754\/Clin.Lab.2015.141238","article-title":"Homocysteine in chronic heart failure","volume":"61","author":"Fournier","year":"2015","journal-title":"Clin. Lab."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"\u0160kovierov\u00e1, H., Vidomanov\u00e1, E., Mahmood, S., Sopkov\u00e1, J., Drgov\u00e1, A., \u010cerve\u0148ov\u00e1, T., Hala\u0161ov\u00e1, E., and Lehotsk\u00fd, J. (2016). The molecular and cellular effect of homocysteine metabolism imbalance on human health. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17101733"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/j.autneu.2013.10.006","article-title":"Moderate hyperhomocysteinemia provokes dysfunction of cardiovascular autonomic system and liver oxidative stress in rats","volume":"180","author":"Mendes","year":"2014","journal-title":"Auton. Neurosci."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1172\/JCI118771","article-title":"Vascular dysfunction in monkeys with diet-induced hyperhomocyst(e)inemia","volume":"98","author":"Lentz","year":"1996","journal-title":"J. Clin. Investig."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2542","DOI":"10.1161\/01.CIR.96.8.2542","article-title":"Hyperhomocysteinemia is a risk factor for arterial endothelial dysfunction in humans","volume":"96","author":"Woo","year":"1997","journal-title":"Circulation"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1161\/hh1101.092180","article-title":"Endothelial dysfunction and elevation of S-adenosyl-homocysteine in cystathionine beta-synthase-deficient mice","volume":"88","author":"Dayal","year":"2001","journal-title":"Circ. Res."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1172\/JCI8342","article-title":"Endothelial dysfunction in a murine model of mild hyperhomocyst(e)inemia","volume":"106","author":"Eberhardt","year":"2000","journal-title":"J. Clin. Investig."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1119","DOI":"10.1161\/01.CIR.95.5.1119","article-title":"Hyperhomocyst(e)inemia is associated with impaired endothelium-dependent vasodilation in humans","volume":"95","author":"Tawakol","year":"1997","journal-title":"Circulation"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.1161\/01.CIR.0000014417.95833.1D","article-title":"Does oral folic acid lower total homocysteine levels and improve endothelial function in children with chronic renal failure?","volume":"105","author":"Kattenhorn","year":"2002","journal-title":"Circulation"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2479","DOI":"10.1161\/01.CIR.102.20.2479","article-title":"Improved vascular endothelial function after oral B vitamins: An effect mediated through reduced concentrations of free plasma homocysteine","volume":"102","author":"Chambers","year":"2000","journal-title":"Circulation"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1016\/j.lfs.2018.10.060","article-title":"Homocysteine accelerates atherosclerosis via inhibiting LXR\u03b1-mediated ABCA1\/ABCG1-dependent cholesterol efflux from macrophages","volume":"214","author":"Jin","year":"2018","journal-title":"Life Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"303","DOI":"10.1016\/j.cjca.2016.12.010","article-title":"Macrophage apoptosis and necrotic core development in atherosclerosis: A rapidly advancing field with clinical relevance to imaging and therapy","volume":"33","author":"Gonzalez","year":"2017","journal-title":"Can. J. Cardiol."},{"key":"ref_14","first-page":"125","article-title":"Homocysteine and oxidative stress markers and inflammation in patients with coronary artery disease","volume":"1","author":"Aparna","year":"2010","journal-title":"Int. J. Biol. Med. Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1007\/s11010-006-9204-9","article-title":"Comparative study on in vitro effects of homocysteine thiolactone and homocysteine on HUVEC cells: Evidence for a stronger proapoptotic and proinflammative homocysteine thiolactone","volume":"291","author":"Kerkeni","year":"2006","journal-title":"Mol. Cell. Biochem."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1016\/j.ajog.2004.01.037","article-title":"Homocysteine thiolactone induces apoptosis in cultured human trophoblasts: A mechanism for homocysteine-mediated placental dysfunction?","volume":"191","author":"Kamudhamas","year":"2004","journal-title":"Am. J. Obstet. Gynecol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"14844","DOI":"10.1074\/jbc.M312948200","article-title":"Homocysteine increases the expression of vascular endothelial growth factor by a mechanism involving endoplasmic reticulum stress and transcription factor ATF4","volume":"279","author":"Roybal","year":"2004","journal-title":"J. Biol. Chem."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"H1206","DOI":"10.1152\/ajpheart.00376.2005","article-title":"Homocysteine causes cerebrovascular leakage in mice","volume":"290","author":"Lominadze","year":"2006","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Esse, R., Barroso, M., Tavares de Almeida, I., and Castro, R. (2019). The contribution of homocysteine metabolism disruption to endothelial dysfunction: State-of-the-art. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20040867"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"H679","DOI":"10.1152\/ajpheart.00145.2003","article-title":"Hyperhomocysteinemia leads to pathologic ventricular hypertrophy in normotensive rats","volume":"285","author":"Joseph","year":"2003","journal-title":"Am. J. Physiol. Heart Circ. Physiol."},{"key":"ref_21","first-page":"175","article-title":"Effect of experimental hyperhomocysteinemia on cardiac structure and function in the rat","volume":"34","author":"Walker","year":"2004","journal-title":"Ann. Clin. Lab. Sci."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1097\/FJC.0000000000000829","article-title":"Effect of high homocysteine level on the severity of coronary heart disease and prognosis after stent implantation","volume":"76","author":"Li","year":"2020","journal-title":"J. Cardiovasc. Pharmacol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1536\/ihj.20-099","article-title":"Elevated homocysteine levels associated with atrial fibrillation and recurrent atrial fibrillation","volume":"61","author":"Rong","year":"2020","journal-title":"Int. Heart J."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1023\/A:1006882014593","article-title":"Reduction-oxidation (redox) and vascular tissue level of homocyst(e)ine in human coronary atherosclerotic lesions and role in vascular extracellular matrix remodeling and vascular tone","volume":"181","author":"Tyagi","year":"1998","journal-title":"Mol. Cell. Biochem."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1955","DOI":"10.1038\/s41467-018-04426-y","article-title":"Methionine metabolism influences genomic architecture and gene expression through H3K4me3 peak width","volume":"9","author":"Dai","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_26","first-page":"126","article-title":"Physical activity, exercise, and physical fitness: Definitions and distinctions for health-related research","volume":"100","author":"Caspersen","year":"1985","journal-title":"Public Health Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1561","DOI":"10.1515\/cclm-2015-1040","article-title":"What do we know about homocysteine and exercise? A review from the literature","volume":"54","author":"Palacios","year":"2016","journal-title":"Clin. Chem. Lab. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"4496","DOI":"10.1210\/jc.2001-012056","article-title":"Exercise decreases plasma total homocysteine in overweight young women with polycystic ovary syndrome","volume":"87","author":"Randeva","year":"2002","journal-title":"J. Clin. Endocrinol. Metab."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"163","DOI":"10.4196\/kjpp.2014.18.2.163","article-title":"Regular exercise training increases the number of endothelial progenitor cells and decreases homocysteine levels in healthy peripheral blood","volume":"18","author":"Choi","year":"2014","journal-title":"Korean J. Physiol. Pharmacol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"328","DOI":"10.1111\/j.1365-2796.2005.01546.x","article-title":"Levels of homocysteine are inversely associated with cardiovascular fitness in women, but not in men: Data from the National Health and Nutrition Examination Survey 1999\u20132002","volume":"258","author":"Kuo","year":"2005","journal-title":"J. Intern. Med."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1001\/archpedi.161.2.166","article-title":"Cardiovascular fitness is negatively associated with homocysteine levels in female adolescents","volume":"161","author":"Ruiz","year":"2007","journal-title":"Arch. Pediatrics Adolesc. Med."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"894","DOI":"10.1016\/j.ypmed.2004.03.012","article-title":"Effects of exercise on emerging and traditional cardiovascular risk factors","volume":"39","author":"Duncan","year":"2004","journal-title":"Prev. Med."},{"key":"ref_33","first-page":"1518","article-title":"Homocysteine Increases during Endurance Exercise","volume":"41","author":"Herrmann","year":"2003","journal-title":"Chem. Lab. Med."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"114","DOI":"10.1159\/000070032","article-title":"Influence of Training Volume and Acute Physical Exercise on the Homocysteine Levels in Endurance-Trained Men: Interactions with Plasma Folate and Vitamin B12","volume":"47","author":"Deibert","year":"2003","journal-title":"Ann. Nutr. Metab."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"476","DOI":"10.1056\/NEJMoa011613","article-title":"Wolf Plasma homocysteine as a risk factor for dementia and Alzheimer\u2019s disease","volume":"346","author":"Seshadri","year":"2002","journal-title":"N. Engl. J. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1161\/CIRCULATIONAHA.106.652693","article-title":"Hyperhomocysteinemia alters cardiac substrate metabolism by impairing nitric oxide bioavailability through oxidative stress","volume":"115","author":"Suematsu","year":"2007","journal-title":"Circulation"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1002\/cbf.2938","article-title":"Homocysteine induces energy imbalance in rat skeletal muscle: Is creatine a protector?","volume":"31","author":"Kolling","year":"2013","journal-title":"Cell Biochem. Funct."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.1152\/physrev.2000.80.3.1107","article-title":"Creatine and creatinine metabolism","volume":"80","author":"Wyss","year":"2000","journal-title":"Physiol. Rev."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Kaplan, P., Tatarkova, Z., Sivonova, M.K., Racay, P., and Lehotsky, J. (2020). Homocysteine and Mitochondria in Cardiovascular and Cerebrovascular Systems. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21207698"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"573","DOI":"10.1007\/s00726-007-0011-8","article-title":"Hydrogen sulfide inhibits myocardial injury induced by homocysteine in rats","volume":"34","author":"Chang","year":"2008","journal-title":"Amino Acids"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1007\/s11010-015-2588-7","article-title":"Effects of mild hyperhomocysteinemia on electron transport chain complexes, oxidative stress, and protein expression in rat cardiac mitochondria","volume":"411","author":"Timkova","year":"2016","journal-title":"Mol. Cell. Biochem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"52","DOI":"10.1016\/j.nutres.2017.01.007","article-title":"1,25-Dihydroxyvitamin D 3 prevents deleterious effects of homocysteine on mitochondrial function and redox status in heart slices","volume":"38","author":"Longoni","year":"2017","journal-title":"Nutr. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"158","DOI":"10.1016\/j.mito.2019.11.004","article-title":"Hydrogen sulfide attenuates hyperhomocysteinemia-induced mitochondrial dysfunctions in brain","volume":"50","author":"Kumar","year":"2020","journal-title":"Mitochondrion"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1007\/s12640-015-9587-z","article-title":"Severe hyperhomocysteinemia decreases respiratory enzyme and Na+-K+ ATPase activities, and leads to mitochondrial alterations in rat amygdala","volume":"29","author":"Kolling","year":"2015","journal-title":"Neurotox. Res."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"48","DOI":"10.1016\/j.neuint.2016.10.001","article-title":"Oxidative stress and mitochondrial dysfunction are the underlying events of dopaminergic neurodegeneration in homocysteine rat model of Parkinson\u2019s disease","volume":"101","author":"Bhattacharjee","year":"2016","journal-title":"Neurochem. Int."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"321","DOI":"10.1016\/j.advenzreg.2003.11.012","article-title":"Methylation demand and homocysteine metabolism","volume":"44","author":"Stead","year":"2004","journal-title":"Adv. Enzym. Regul."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1331","DOI":"10.1046\/j.1523-1755.2003.00206.x","article-title":"Creatine supplementation decrease homocysteine in an animal model of uremia","volume":"64","author":"Taes","year":"2003","journal-title":"Kidney Int."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"5","DOI":"10.1054\/mehy.1999.1014","article-title":"Supplemental creatine may decrease serum homocysteine and abolish the homocysteine \u2018gender gap\u2019 by suppressing endogenous creatine synthesis","volume":"56","author":"Mccarty","year":"2001","journal-title":"Med. Hypotheses"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"557","DOI":"10.1002\/ana.20419","article-title":"Creatine metabolism in combined methylmalonic aciduria and homocystinuria","volume":"57","author":"Bodamer","year":"2005","journal-title":"Ann. Neurol."},{"key":"ref_50","first-page":"237","article-title":"The effect of high intensity interval training on cardioprotection against ischemia-reperfusion injury in Wistar rats","volume":"14","author":"Rahimi","year":"2015","journal-title":"EXCLI J."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"923","DOI":"10.2165\/11317870-000000000-00000","article-title":"Exercise preconditioning of the myocardium","volume":"39","author":"Kavazis","year":"2009","journal-title":"Sports Med."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.biopha.2008.04.001","article-title":"Immunohistochemical detection of N-homocysteinylated proteins in humans and mice","volume":"62","author":"Stanger","year":"2008","journal-title":"Biomed. Pharmacother."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"289","DOI":"10.2174\/156720207782446324","article-title":"Cardiac dys-synchronization and arrhythmia in hyperhomocysteinemia","volume":"4","author":"Moshal","year":"2007","journal-title":"Curr. Neurovasc. Res."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1002\/ajmg.c.30293","article-title":"Hypermethioninemias of genetic and nongenetic origin: A review","volume":"157","author":"Mudd","year":"2011","journal-title":"Am. J. Med. Genet. C"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1716","DOI":"10.1016\/j.freeradbiomed.2012.02.033","article-title":"Methionine excess in diet induces acute lethal hepatitis in mice lacking cystathionine \u03b3-lyase, an animal model of cystathioninuria","volume":"52","author":"Yamada","year":"2012","journal-title":"Free Radic. Biol. Med."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1007\/s11010-018-3311-2","article-title":"Subchronic methionine load induces oxidative stress and provokes biochemical and histological changes in the rat liver tissue","volume":"448","author":"Borozan","year":"2018","journal-title":"Mol. Cell. Biochem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"589","DOI":"10.2174\/157016110792006987","article-title":"Fenofibrate, homocysteine and renal function","volume":"8","author":"Foucher","year":"2010","journal-title":"Curr. Vasc. Pharmacol."},{"key":"ref_58","doi-asserted-by":"crossref","unstructured":"Dong, Q., Tang, G., He, M., Cai, Y., Cai, Y., Xing, H., Sun, L., Li, J., Zhang, Y., and Fan, F. (2012). Methylenetetrahydrofolate reductase C677T polymorphism is associated with estimated glomerular filtration rate in hypertensive Chinese males. BMC Med. Genet., 13.","DOI":"10.1186\/1471-2350-13-74"},{"key":"ref_59","doi-asserted-by":"crossref","unstructured":"Marti, F., Vollenweider, P., Marques-Vidal, P.M., Mooser, V., Waeber, G., Paccaud, F., and Bochud, M. (2011). Hyperhomocysteinemia is independently associated with albuminuria in the population-based CoLaus study. BMC Public Health, 11.","DOI":"10.1186\/1471-2458-11-733"},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"873","DOI":"10.1093\/clinchem\/40.6.873","article-title":"Rapid HPLC determination of total homocysteine and other thiols in serum and plasma: Sex differences and correlation with and folate concentrations in healthy subjects","volume":"40","author":"Jacobsen","year":"1994","journal-title":"Clin. Chem."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"S61","DOI":"10.4149\/gpb_2011_SI1_61","article-title":"Response of secretory pathways Ca2+ ATPase gene expression to hyperhomocysteinemia and\/or ischemic preconditioning in rat cerebral cortex and hippocampus","volume":"30","author":"Pavlikova","year":"2011","journal-title":"Gen. Physiol. Biophys."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1111\/j.1753-0407.2008.00003.x","article-title":"Exercise test and glucose homeostasis in rats treated with alloxan during the neonatal period or fed a high calorie diet","volume":"1","author":"Contarteze","year":"2009","journal-title":"J. Diabetes"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1016\/S1388-9842(99)00048-3","article-title":"Altered balance between matrix gelatinases (MMP-2 and MMP-9) and their tissue inhibitors in human dilated cardiomyopathy: Potential role of MMP-9 in myosin-heavy chain degradation","volume":"1","author":"Buhler","year":"1999","journal-title":"Eur. J. Heart Fail."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","article-title":"A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding","volume":"72","author":"Bradford","year":"1976","journal-title":"Anal. Biochem."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/0009-8981(79)90036-6","article-title":"Interference of the measurement of lactate dehydrogenase (LDH) activity in human serum and plasma by LDH from blood cells","volume":"99","author":"Hollaar","year":"1979","journal-title":"Clin. Chim. Acta"},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"2106","DOI":"10.1016\/S0021-9258(19)41689-X","article-title":"Kinetic studies on pig heart cytoplasmic malate dehydrogenase","volume":"250","author":"Frieden","year":"1975","journal-title":"J. Biol. Chem."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"765","DOI":"10.1136\/jcp.18.6.765","article-title":"Lactic dehydrogenase isoenzymes in normal and pathological spinal fluids","volume":"18","author":"Cunningham","year":"1965","journal-title":"J. Clin. Pathol."},{"key":"ref_68","first-page":"337","article-title":"Zymographic demonstration of lactate and malate dehydrogenases isoenzymes in the rodent salivary glands","volume":"22","author":"Yoshimura","year":"1970","journal-title":"Histochemie"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1006\/abio.1994.1186","article-title":"Quantitative zymography detection of picogram quantities of gelatinases","volume":"218","author":"Kleiner","year":"1994","journal-title":"Anal. Biochem."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/13\/6792\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:23:14Z","timestamp":1760163794000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/13\/6792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,24]]},"references-count":69,"journal-issue":{"issue":"13","published-online":{"date-parts":[[2021,7]]}},"alternative-id":["ijms22136792"],"URL":"https:\/\/doi.org\/10.3390\/ijms22136792","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,6,24]]}}}