{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T14:48:10Z","timestamp":1789138090731,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"13","license":[{"start":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T00:00:00Z","timestamp":1624838400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004747","name":"Katolicki Uniwersytet Lubelski Jana Pawla II","doi-asserted-by":"publisher","award":["Internal grant for Ilona Sadok"],"award-info":[{"award-number":["Internal grant for Ilona Sadok"]}],"id":[{"id":"10.13039\/501100004747","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["OPUS13 grant (2017\/25\/B\/NZ4\/01198) for Magdalena Staniszewska"],"award-info":[{"award-number":["OPUS13 grant (2017\/25\/B\/NZ4\/01198) for Magdalena Staniszewska"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Metabolites and enzymes involved in the kynurenine pathway (KP) are highly promising targets for cancer treatment, including gastrointestinal tract diseases. Thus, accurate quantification of these compounds in body fluids becomes increasingly important. The aim of this study was the development and validation of the UHPLC-ESI-MS\/MS methods for targeted quantification of biologically important KP substrates (tryptophan and nicotinamide) and metabolites(kynurenines) in samples of serum and peritoneal fluid from gastric cancer patients. The serum samples were simply pretreated with trichloroacetic acid to precipitate proteins. The peritoneal fluid was purified by solid-phase extraction before analysis. Validation was carried out for both matrices independently. Analysis of the samples from gastric cancer patients showed different accumulations of tryptophan and its metabolites in different biofluids of the same patient. The protocols will be used for the evaluation of tryptophan and kynurenines in blood and peritoneal fluid to determine correlation with the clinicopathological status of gastric cancer or the disease\u2019s prognosis.<\/jats:p>","DOI":"10.3390\/ijms22136972","type":"journal-article","created":{"date-parts":[[2021,6,28]],"date-time":"2021-06-28T11:45:40Z","timestamp":1624880740000},"page":"6972","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["UHPLC-ESI-MS\/MS Quantification of Relevant Substrates and Metabolites of the Kynurenine Pathway Present in Serum and Peritoneal Fluid from Gastric Cancer Patients\u2014Method Development and Validation"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1154-7581","authenticated-orcid":false,"given":"Ilona","family":"Sadok","sequence":"first","affiliation":[{"name":"Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, Faculty of Science and Health, The John Paul II Catholic University of Lublin, Konstantyn\u00f3w 1J, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3945-7377","authenticated-orcid":false,"given":"Katarzyna","family":"J\u0119druchniewicz","sequence":"additional","affiliation":[{"name":"Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, Faculty of Science and Health, The John Paul II Catholic University of Lublin, Konstantyn\u00f3w 1J, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2555-2114","authenticated-orcid":false,"given":"Karol","family":"Rawicz-Pruszy\u0144ski","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology, Medical University of Lublin, Radziwi\u0142\u0142owska 13, 20-080 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1119-2397","authenticated-orcid":false,"given":"Magdalena","family":"Staniszewska","sequence":"additional","affiliation":[{"name":"Laboratory of Separation and Spectroscopic Method Applications, Centre for Interdisciplinary Research, Faculty of Science and Health, The John Paul II Catholic University of Lublin, Konstantyn\u00f3w 1J, 20-708 Lublin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1002\/ijc.26430","article-title":"Carcinogenesis, prevention and early detection of gastric cancer: Where we are and where we should go","volume":"130","author":"Agudo","year":"2012","journal-title":"Int. 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