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Genetic variations of ABCB4 are associated with several rare cholestatic diseases. The available treatments are not efficient for a significant proportion of patients with ABCB4-related diseases and liver transplantation is often required. The development of novel therapies requires a deep understanding of the molecular mechanisms regulating ABCB4 expression, intracellular traffic, and function. Using an immunoprecipitation approach combined with mass spectrometry analyses, we have identified the small GTPase RAB10 as a novel molecular partner of ABCB4. Our results indicate that the overexpression of wild type RAB10 or its dominant-active mutant significantly increases the amount of ABCB4 at the plasma membrane expression and its phosphatidylcholine floppase function. Contrariwise, RAB10 silencing induces the intracellular retention of ABCB4 and then indirectly diminishes its secretory function. Taken together, our findings suggest that RAB10 regulates the plasma membrane targeting of ABCB4 and consequently its capacity to mediate phosphatidylcholine secretion.<\/jats:p>","DOI":"10.3390\/ijms22137087","type":"journal-article","created":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T10:03:19Z","timestamp":1625047399000},"page":"7087","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["RAB10 Interacts with ABCB4 and Regulates Its Intracellular Traffic"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2105-1491","authenticated-orcid":false,"given":"Amel","family":"Ben Saad","sequence":"first","affiliation":[{"name":"Inserm, Universit\u00e9 Paris-Saclay, Physiopathog\u00e9n\u00e8se et traitement des maladies du foie, UMR_S 1193, Hepatinov, 91400 Orsay, France"},{"name":"Inserm, Sorbonne Universit\u00e9, Centre de Recherche Saint-Antoine (CRSA), UMR_S 938, Institute of 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