{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:11:39Z","timestamp":1760235099061,"version":"build-2065373602"},"reference-count":71,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T00:00:00Z","timestamp":1627430400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Herein, for the first time, the potential relationships between the cytoskeleton-associated proteins DAAM1 and PREP with different testicular disorders, such as classic seminoma (CS), Leydig cell tumor (LCT), and Sertoli cell-only syndrome (SOS), were evaluated. Six CS, two LCT, and two SOS tissue samples were obtained during inguinal exploration in patients with a suspect testis tumor based on clinical examination and ultrasonography. DAAM1 and PREP protein levels and immunofluorescent localization were analyzed. An increased DAAM1 protein level in CS and SOS as compared to non-pathological (NP) tissue was observed, while LCT showed no significant differences. Conversely, PREP protein level increased in LCT, while it decreased in CS and SOS compared to NP tissue. These results were strongly supported by the immunofluorescence staining, revealing an altered localization and signal intensity of DAAM1 and PREP in the analyzed samples, highlighting a perturbed cytoarchitecture. Interestingly, in LCT spermatogonia, a specific DAAM1 nuclear localization was found, probably due to an enhanced testosterone production, as confirmed by the increased protein levels of steroidogenic enzymes. Finally, although further studies are needed to verify the involvement of other formins and microtubule-associated proteins, this report raised the opportunity to indicate DAAM1 and PREP as new potential markers, supporting the cytoskeleton dynamics changes occurring during normal and\/or pathological cell differentiation.<\/jats:p>","DOI":"10.3390\/ijms22158094","type":"journal-article","created":{"date-parts":[[2021,7,28]],"date-time":"2021-07-28T11:12:52Z","timestamp":1627470772000},"page":"8094","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Preliminary Investigation on the Involvement of Cytoskeleton-Related Proteins, DAAM1 and PREP, in Human Testicular Disorders"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7357-5353","authenticated-orcid":false,"given":"Massimo","family":"Venditti","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Sez, Fisiologia Umana e Funzioni Biologiche Integrate \u201cF. Bottazzi\u201d, Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d, Via Costantinopoli 16, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davide","family":"Arcaniolo","sequence":"additional","affiliation":[{"name":"Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d, Via Luigi De Crecchio 2, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marco","family":"De Sio","sequence":"additional","affiliation":[{"name":"Dipartimento della Donna, del Bambino e di Chirurgia Generale e Specialistica, Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d, Via Luigi De Crecchio 2, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1007-7840","authenticated-orcid":false,"given":"Sergio","family":"Minucci","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina Sperimentale, Sez, Fisiologia Umana e Funzioni Biologiche Integrate \u201cF. Bottazzi\u201d, Universit\u00e0 degli Studi della Campania \u201cLuigi Vanvitelli\u201d, Via Costantinopoli 16, 80138 Napoli, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"755","DOI":"10.2174\/138955707781024472","article-title":"Molecular Targets for the Treatment of Testicular Germ Cell Tumors","volume":"7","author":"Chieffi","year":"2007","journal-title":"Mini Rev. Med. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"53","DOI":"10.2174\/1574892814666190111120023","article-title":"New Anti-Cancer Strategies in Testicular Germ Cell Tumors","volume":"14","author":"Chieffi","year":"2019","journal-title":"Recent Pat. Anti-Cancer Drug Discov."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"De Martino, M., Chieffi, P., and Esposito, F. (2021). miRNAs and Biomarkers in Testicular Germ Cell Tumors: An Update. 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