{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T19:31:51Z","timestamp":1791487911432,"version":"4.3.4"},"reference-count":13,"publisher":"MDPI AG","issue":"15","license":[{"start":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T00:00:00Z","timestamp":1627603200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The distribution of differential extracellular matrix (ECM) in the lateral and medial menisci can contribute to knee instability, and changes in the meniscus tissue can lead to joint disease. Thus, deep proteomic identification of the lateral and medial meniscus cartilage is expected to provide important information for treatment and diagnosis of various knee joint diseases. We investigated the proteomic profiles of 12 lateral\/medial meniscus pairs obtained from excess tissue of osteoarthritis patients who underwent knee arthroscopy surgery using mass spectrometry-based techniques and measured 75 ECM protein levels in the lesions using a multiple reaction monitoring (MRM) assay we developed. A total of 906 meniscus proteins with a 1% false discovery rate (FDR) was identified through a tandem mass tag (TMT) analysis showing that the lateral and medial menisci had similar protein expression profiles. A total of 131 ECM-related proteins was included in meniscus tissues such as collagen, fibronectin, and laminin. Our data showed that 14 ECM protein levels were differentially expressed in lateral and medial lesions (p &lt; 0.05). We present the proteomic characterization of meniscal tissue with mass spectrometry-based comparative proteomic analysis and developed an MRM-based assay of ECM proteins correlated with tissue regeneration. The mass spectrometry dataset has been deposited to the MassIVE repository with the dataset identifier MSV000087753.<\/jats:p>","DOI":"10.3390\/ijms22158181","type":"journal-article","created":{"date-parts":[[2021,7,30]],"date-time":"2021-07-30T03:46:42Z","timestamp":1627616802000},"page":"8181","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Proteomic Analysis of the Meniscus Cartilage in Osteoarthritis"],"prefix":"10.3390","volume":"22","author":[{"given":"Jisook","family":"Park","sequence":"first","affiliation":[{"name":"Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4590-4989","authenticated-orcid":false,"given":"Hyun-Seung","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eun-Bi","family":"Go","sequence":"additional","affiliation":[{"name":"Samsung Biomedical Research Institute, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0855-6516","authenticated-orcid":false,"given":"Ju Yeon","family":"Lee","sequence":"additional","affiliation":[{"name":"Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Young","family":"Kim","sequence":"additional","affiliation":[{"name":"Research Center for Bioconvergence Analysis, Korea Basic Science Institute, Cheongju 28119, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7595-4042","authenticated-orcid":false,"given":"Soo-Youn","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"},{"name":"Department of Clinical Pharmacology and Therapeutics, Samsung Medical Center, Seoul 06351, Korea"},{"name":"Department of Health Science and Technology, Samsung Advanced Institute of Health Science and Technology, Sungkyunkwan University, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dae-Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Orthopedic Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul 06351, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,7,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"7411","DOI":"10.1016\/j.biomaterials.2011.06.037","article-title":"The knee meniscus: Structure-function, pathophysiology, current repair techniques, and prospects for regeneration","volume":"32","author":"Makris","year":"2011","journal-title":"Biomaterials"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1454","DOI":"10.1136\/bjsports-2018-100022","article-title":"Knee osteoarthritis risk is increased 4-6 fold after knee injury\u2014A systematic review and meta-analysis","volume":"53","author":"Poulsen","year":"2019","journal-title":"Br. 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