{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,9]],"date-time":"2026-05-09T05:39:21Z","timestamp":1778305161180,"version":"3.51.4"},"reference-count":65,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T00:00:00Z","timestamp":1628035200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000615","name":"Fight for Sight UK","doi-asserted-by":"publisher","award":["1423\/1424"],"award-info":[{"award-number":["1423\/1424"]}],"id":[{"id":"10.13039\/501100000615","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Wellcome Trust","doi-asserted-by":"publisher","award":["ISSF3"],"award-info":[{"award-number":["ISSF3"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Retinal ganglion cells (RGCs) undergo dendritic pruning in a variety of neurodegenerative diseases, including glaucoma and autosomal dominant optic atrophy (ADOA). Axotomising RGCs by severing the optic nerve generates an acute model of RGC dendropathy, which can be utilized to assess the therapeutic potential of treatments for RGC degeneration. Photobiomodulation (PBM) with red light provided neuroprotection to RGCs when administered ex vivo to wild-type retinal explants. In the current study, we used aged (13\u201315-month-old) wild-type and heterozygous B6;C3-Opa1Q285STOP (Opa1+\/\u2212) mice, a model of ADOA exhibiting RGC dendropathy. These mice were pre-treated with 4 J\/cm2 of 670 nm light for five consecutive days before the eyes were enucleated and the retinas flat-mounted into explant cultures for 0-, 8- or 16-h ex vivo. RGCs were imaged by confocal microscopy, and their dendritic architecture was quantified by Sholl analysis. In vivo 670 nm light pretreatment inhibited the RGC dendropathy observed in untreated wild-type retinas over 16 h ex vivo and inhibited dendropathy in ON-center RGCs in wild-type but not Opa1+\/\u2212 retinas. Immunohistochemistry revealed that aged Opa1+\/\u2212 RGCs exhibited increased nitrosative damage alongside significantly lower activation of NF-\u03baB and upregulation of DJ-1. PBM restored NF-\u03baB activation in Opa1+\/\u2212 RGCs and enhanced DJ-1 expression in both genotypes, indicating a potential molecular mechanism priming the retina to resist future oxidative insult. These data support the potential of PBM as a treatment for diseases involving RGC degeneration.<\/jats:p>","DOI":"10.3390\/ijms22168380","type":"journal-article","created":{"date-parts":[[2021,8,4]],"date-time":"2021-08-04T08:47:52Z","timestamp":1628066872000},"page":"8380","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Red Light Irradiation In Vivo Upregulates DJ-1 in the Retinal Ganglion Cell Layer and Protects against Axotomy-Related Dendritic Pruning"],"prefix":"10.3390","volume":"22","author":[{"given":"Kathy","family":"Beirne","sequence":"first","affiliation":[{"name":"School of Optometry and Vision Sciences, Cardiff University, Cardiff CF24 4HQ, UK"},{"name":"Cardiff Institute for Tissue Engineering and Repair, Cardiff University, Cardiff CF10 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6405-4962","authenticated-orcid":false,"given":"Thomas J.","family":"Freeman","sequence":"additional","affiliation":[{"name":"School of Optometry and Vision Sciences, Cardiff University, Cardiff CF24 4HQ, UK"},{"name":"Cardiff Institute for Tissue Engineering and Repair, Cardiff University, Cardiff CF10 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malgorzata","family":"Rozanowska","sequence":"additional","affiliation":[{"name":"School of Optometry and Vision Sciences, Cardiff University, Cardiff CF24 4HQ, UK"},{"name":"Cardiff Institute for Tissue Engineering and Repair, Cardiff University, Cardiff CF10 3NB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7680-9135","authenticated-orcid":false,"given":"Marcela","family":"Votruba","sequence":"additional","affiliation":[{"name":"School of Optometry and Vision Sciences, Cardiff University, Cardiff CF24 4HQ, UK"},{"name":"Cardiff Institute for Tissue Engineering and Repair, Cardiff University, Cardiff CF10 3NB, UK"},{"name":"Cardiff Eye Unit, University Hospital of Wales, Cardiff CF14 4XW, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,4]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1136\/jmg.35.10.793","article-title":"Clinical features, molecular genetics, and pathophysiology of dominant optic atrophy","volume":"35","author":"Votruba","year":"1998","journal-title":"J. Med. Genet."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1111\/j.1442-9071.2012.02789.x","article-title":"Retina ganglion cell degeneration in glaucoma: An opportunity missed? A review","volume":"40","author":"Morgan","year":"2012","journal-title":"Clin. Exp. Ophthalmol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1186\/s40478-020-01094-2","article-title":"Retinal ganglion cell degeneration correlates with hippocampal spine loss in experimental Alzheimer\u2019s disease","volume":"8","author":"Bevan","year":"2020","journal-title":"Acta Neuropathol. Commun."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"710","DOI":"10.3389\/fneur.2017.00710","article-title":"Patterns of retinal ganglion cell damage in neurodegenerative disorders: Parvocellular vs magnocellular degeneration in optical coherence tomography studies","volume":"8","author":"Carelli","year":"2017","journal-title":"Front. Neurol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1002\/mds.26486","article-title":"Spectral-domain optical coherence tomography as a potential biomarker in Huntington\u2019s disease","volume":"31","author":"Andrade","year":"2016","journal-title":"Mov. Disord."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1016\/j.phrs.2018.02.018","article-title":"OPA1: How much do we know to approach therapy?","volume":"131","author":"Fogazza","year":"2018","journal-title":"Pharm. Res."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"300","DOI":"10.1111\/j.1755-3768.1983.tb01424.x","article-title":"Histopathology of eye, optic nerve and brain in a case of dominant optic atrophy","volume":"61","author":"Kjer","year":"1983","journal-title":"Acta Ophthalmol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"4217","DOI":"10.1167\/iovs.03-1261","article-title":"OPA1, the disease gene for autosomal dominant optic atrophy, is specifically expressed in ganglion cells and intrinsic neurons of the retina","volume":"45","author":"Pesch","year":"2004","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100935","DOI":"10.1016\/j.preteyeres.2020.100935","article-title":"Dominant optic atrophy: Culprit mitochondria in the optic nerve","volume":"83","author":"Lenaers","year":"2020","journal-title":"Prog. Retin. Eye Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1307","DOI":"10.1093\/hmg\/ddm079","article-title":"Opa1 deficiency in a mouse model of autosomal dominant optic atrophy impairs mitochondrial morphology, optic nerve structure and visual function","volume":"16","author":"Davies","year":"2007","journal-title":"Hum. Mol. Genet."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2942","DOI":"10.1093\/brain\/awq218","article-title":"Opa1 deficiency in a mouse model of dominant optic atrophy leads to retinal ganglion cell dendropathy","volume":"133","author":"Williams","year":"2010","journal-title":"Brain"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1029","DOI":"10.1093\/brain\/awm005","article-title":"A splice site mutation in the murine Opa1 gene features pathology of autosomal dominant optic atrophy","volume":"130","author":"Alavi","year":"2007","journal-title":"Brain"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1424","DOI":"10.1167\/iovs.09-3606","article-title":"Electrophysiological and histologic assessment of retinal ganglion cell fate in a mouse model for OPA1-associated autosomal dominant optic atrophy","volume":"51","author":"Heiduschka","year":"2010","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1167\/iovs.61.6.42","article-title":"Opa1 deficiency leads to diminished mitochondrial bioenergetics with compensatory increased mitochondrial motility","volume":"61","author":"Sun","year":"2020","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"85","DOI":"10.1016\/j.mito.2017.05.002","article-title":"Photostimulation of mitochondria as a treatment for retinal neurodegeneration","volume":"36","author":"Beirne","year":"2017","journal-title":"Mitochondrion"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Begum, R., Powner, M.B., Hudson, N., Hogg, C., and Jeffery, G. (2013). Treatment with 670 Nm light up regulates cytochrome C oxidase expression and reduces inflammation in an age-related macular degeneration model. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0057828"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Samanta, A., Aziz, A.A., Jhingan, M., Singh, S.R., Khanani, A.M., and Chhablani, J. (2021). Emerging therapies in nonexudative age-related macular degeneration in 2020. Asia Pac. J. Ophthalmol.","DOI":"10.1097\/APO.0000000000000355"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"e72135","DOI":"10.1371\/journal.pone.0072135","article-title":"670nm photobiomodulation as a novel protection against retinopathy of prematurity: Evidence from oxygen induced retinopathy models","volume":"8","author":"Natoli","year":"2013","journal-title":"PLoS ONE"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"291","DOI":"10.2337\/db17-0803","article-title":"Photobiomodulation inhibits long-term structural and functional lesions of diabetic retinopathy","volume":"67","author":"Cheng","year":"2018","journal-title":"Diabetes"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"66","DOI":"10.1016\/j.neurobiolaging.2017.01.001","article-title":"Aging retinal function is improved by near infrared light (670 Nm) that is associated with corrected mitochondrial decline","volume":"52","author":"Sivapathasuntharam","year":"2017","journal-title":"Neurobiol. Aging"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1016\/j.neuint.2019.02.018","article-title":"Blue light exacerbates and red light counteracts negative insults to retinal ganglion cells in situ and R28 cells in vitro","volume":"125","author":"Osborne","year":"2019","journal-title":"Neurochem. Int."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3582","DOI":"10.1167\/iovs.10-6664","article-title":"Photobiomodulation protects the retina from light-induced photoreceptor degeneration","volume":"52","author":"Albarracin","year":"2011","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.exer.2018.01.011","article-title":"670nm light treatment following retinal injury modulates M\u00fcller cell gliosis: Evidence from in vivo and in vitro stress models","volume":"169","author":"Lu","year":"2018","journal-title":"Exp. Eye Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e270","DOI":"10.1111\/aos.13354","article-title":"Photobiomodulation reduces drusen volume and improves visual acuity and contrast sensitivity in dry age-related macular degeneration","volume":"95","author":"Merry","year":"2017","journal-title":"Acta Ophthalmol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1013","DOI":"10.1136\/bjophthalmol-2013-304477","article-title":"Photobiomodulation in the treatment of patients with non-center-involving diabetic macular oedema","volume":"98","author":"Tang","year":"2014","journal-title":"Br. J. Ophthalmol."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Grewal, M.K., Sivapathasuntharam, C., Chandra, S., Gurudas, S., Chong, V., Bird, A., Jeffery, G., and Sivaprasad, S. (2020). A pilot study evaluating the effects of 670 Nm photobiomodulation in healthy ageing and age-related macular degeneration. J. Clin. Med., 9.","DOI":"10.3390\/jcm9041001"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e49","DOI":"10.1093\/gerona\/glaa155","article-title":"Optically improved mitochondrial function redeems aged human visual decline","volume":"75","author":"Shinhmar","year":"2020","journal-title":"J. Gerontol. A Biol. Sci. Med. Sci."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1471","DOI":"10.1097\/IAE.0000000000002632","article-title":"A double-masked, randomized, sham-controlled, single-center study with photobiomodulation for the treatment of dry age-related macular degeneration","volume":"40","author":"Markowitz","year":"2020","journal-title":"Retina"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1111\/php.12864","article-title":"Mechanisms and mitochondrial redox signaling in photobiomodulation","volume":"94","author":"Hamblin","year":"2018","journal-title":"Photochem. Photobiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1091","DOI":"10.1111\/j.1751-1097.2008.00394.x","article-title":"Mitochondrial signaling in mammalian cells activated by red and near-IR radiation","volume":"84","author":"Karu","year":"2008","journal-title":"Photochem. Photobiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1882","DOI":"10.1016\/j.bbabio.2014.08.005","article-title":"Re-evaluation of the near infrared spectra of mitochondrial cytochrome c oxidase: Implications for non invasive in vivo monitoring of tissues","volume":"1837","author":"Mason","year":"2014","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.exer.2016.09.006","article-title":"Optical monitoring of retinal respiration in real time: 670 Nm light increases the redox state of mitochondria","volume":"152","author":"Kaynezhad","year":"2016","journal-title":"Exp. Eye Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"495","DOI":"10.1007\/s10103-018-2623-5","article-title":"Photobiomodulation with 808-Nm diode laser light promotes wound healing of human endothelial cells through increased reactive oxygen species production stimulating mitochondrial oxidative phosphorylation","volume":"34","author":"Amaroli","year":"2019","journal-title":"Lasers Med. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"829","DOI":"10.1002\/jbio.201200157","article-title":"Low-level laser therapy (LLLT) reduces oxidative stress in primary cortical neurons in vitro","volume":"6","author":"Huang","year":"2013","journal-title":"J. Biophotonics"},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Chen, A.C.-H., Arany, P.R., Huang, Y.-Y., Tomkinson, E.M., Sharma, S.K., Kharkwal, G.B., Saleem, T., Mooney, D., Yull, F.E., and Blackwell, T.S. (2011). Low-level laser therapy activates NF-KB via generation of reactive oxygen species in mouse embryonic fibroblasts. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0022453"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1016\/j.neuro.2008.01.007","article-title":"Oxidative insults induce DJ-1 upregulation and redistribution: Implications for neuroprotection","volume":"29","author":"Lev","year":"2008","journal-title":"Neurotoxicology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"13511","DOI":"10.1523\/JNEUROSCI.3457-08.2008","article-title":"Neuroprotective effects of near-infrared light in an in vivo model of mitochondrial optic neuropathy","volume":"28","author":"Rojas","year":"2008","journal-title":"J. Neurosci."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1016\/j.neuroscience.2008.03.042","article-title":"Near-infrared light via light-emitting diode treatment is therapeutic against rotenone- and 1-methyl-4-phenylpyridinium ion-induced neurotoxicity","volume":"153","author":"Liang","year":"2008","journal-title":"Neuroscience"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"624","DOI":"10.1111\/php.12606","article-title":"Red light treatment in an axotomy model of neurodegeneration","volume":"92","author":"Beirne","year":"2016","journal-title":"Photochem. Photobiol."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Magharious, M.M., D\u2019Onofrio, P.M., and Koeberle, P.D. (2011). Optic nerve transection: A model of adult neuron apoptosis in the central nervous system. J. Vis. Exp.","DOI":"10.3791\/2241-v"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"639","DOI":"10.1016\/j.neuroscience.2005.12.047","article-title":"Photobiomodulation partially rescues visual cortical neurons from cyanide-induced apoptosis","volume":"139","author":"Liang","year":"2006","journal-title":"Neuroscience"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e201800359","DOI":"10.1002\/jbio.201800359","article-title":"Photobiomodulation preconditioning prevents cognitive impairment in a neonatal rat model of hypoxia-ischemia","volume":"12","author":"Yang","year":"2019","journal-title":"J. Biophotonics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1042\/EBC20190052","article-title":"3-nitrotyrosine and related derivatives in proteins: Precursors, radical intermediates and impact in function","volume":"64","author":"Campolo","year":"2020","journal-title":"Essays Biochem."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1146\/annurev-pharmtox-011112-140320","article-title":"Role of Nrf2 in oxidative stress and toxicity","volume":"53","author":"Ma","year":"2013","journal-title":"Annu. Rev. Pharmacol. Toxicol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.1128\/MCB.25.11.4501-4513.2005","article-title":"Keap1 regulates the oxidation-sensitive shuttling of Nrf2 into and out of the nucleus via a Crm1-dependent nuclear export mechanism","volume":"25","author":"Velichkova","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.jphotobiol.2016.08.049","article-title":"Near-infrared laser increases MDPC-23 odontoblast-like cells proliferation by activating redox sensitive pathways","volume":"164","author":"Rizzi","year":"2016","journal-title":"J. Photochem. Photobiol. B"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"540","DOI":"10.1126\/science.3140380","article-title":"I kappa B: A specific inhibitor of the NF-kappa B transcription factor","volume":"242","author":"Baeuerle","year":"1988","journal-title":"Science"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1007\/s10863-019-09798-4","article-title":"Role of DJ-1 in the mechanism of pathogenesis of Parkinson\u2019s disease","volume":"51","author":"Dolgacheva","year":"2019","journal-title":"J. Bioenerg. Biomembr."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Tang, S., Le, P.K., Tse, S., Wallace, D.C., and Huang, T. (2009). Heterozygous mutation of Opa1 in drosophila shortens lifespan mediated through increased reactive oxygen species production. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0004492"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Ashworth, B.E., Stephens, E., Bartlett, C.A., Serghiou, S., Giacci, M.K., Williams, A., Hart, N.S., and Fitzgerald, M. (2016). Comparative assessment of phototherapy protocols for reduction of oxidative stress in partially transected spinal cord slices undergoing secondary degeneration. BMC Neurosci., 17.","DOI":"10.1186\/s12868-016-0259-6"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Muili, K.A., Gopalakrishnan, S., Eells, J.T., and Lyons, J.-A. (2013). Photobiomodulation induced by 670 Nm light ameliorates MOG35-55 induced EAE in female C57BL\/6 mice: A role for remediation of nitrosative stress. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0067358"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1109\/JSTQE.2016.2561201","article-title":"Proposed mechanisms of photobiomodulation or low-level light therapy","volume":"22","author":"Hamblin","year":"2016","journal-title":"IEEE J. Sel. Top. Quantum Electron."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41419-019-1679-x","article-title":"Parkinson\u2019s disease protein DJ-1 regulates ATP synthase protein components to increase neuronal process outgrowth","volume":"10","author":"Chen","year":"2019","journal-title":"Cell Death Dis."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"13719","DOI":"10.1038\/s41598-020-70486-0","article-title":"Deletion of DJ-1 in rats affects protein abundance and mitochondrial function at the synapse","volume":"10","author":"Almikhlafi","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.1167\/iovs.19-27212","article-title":"Mice with a combined deficiency of superoxide dismutase 1 (Sod1), DJ-1 (Park7), and parkin (Prkn) develop spontaneous retinal degeneration with aging","volume":"60","author":"Zhu","year":"2019","journal-title":"Investig. Ophthalmol. Vis. Sci."},{"key":"ref_56","doi-asserted-by":"crossref","unstructured":"Upadhyay, M., Milliner, C., Bell, B.A., and Bonilha, V.L. (2020). Oxidative stress in the retina and retinal pigment epithelium (RPE): Role of aging, and DJ-1. Redox Biol., 37.","DOI":"10.1016\/j.redox.2020.101623"},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"107830","DOI":"10.1016\/j.exer.2019.107830","article-title":"DJ-1\/PARK7 inhibits high glucose-induced oxidative stress to prevent retinal pericyte apoptosis via the PI3K\/AKT\/MTOR signaling pathway","volume":"189","author":"Zeng","year":"2019","journal-title":"Exp. Eye Res."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"134361","DOI":"10.1016\/j.neulet.2019.134361","article-title":"A role for DJ-1 against oxidative stress in the mammalian retina","volume":"708","author":"Uribe","year":"2019","journal-title":"Neurosci. Lett."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"81","DOI":"10.1016\/j.cotox.2017.11.002","article-title":"NF-\u039aB in oxidative stress","volume":"7","author":"Lingappan","year":"2018","journal-title":"Curr. Opin. Toxicol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1016\/j.freeradbiomed.2008.09.021","article-title":"Sustained oxidative stress inhibits NF-\u039aB activation partially via inactivating the proteasome","volume":"46","author":"Wu","year":"2009","journal-title":"Free Radic. Biol. Med."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"42321","DOI":"10.1074\/jbc.M308192200","article-title":"Nitric oxide activates diverse signaling pathways to regulate gene expression","volume":"278","author":"Hemish","year":"2003","journal-title":"J. Biol. Chem."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1186\/s13041-018-0412-5","article-title":"Digestion of the glycosaminoglycan extracellular matrix by chondroitinase ABC supports retinal ganglion cell dendritic preservation in a rodent model of experimental glaucoma","volume":"11","author":"Tribble","year":"2018","journal-title":"Mol. Brain"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"2453","DOI":"10.1093\/bioinformatics\/btr390","article-title":"Simple Neurite Tracer: Open source software for reconstruction, visualization and analysis of neuronal processes","volume":"27","author":"Longair","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_64","first-page":"387","article-title":"Dendritic organization in the neurons of the visual and motor cortices of the cat","volume":"87","author":"Sholl","year":"1953","journal-title":"J. Anat."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: An Open-Source Platform for Biological-Image Analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8380\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:40:25Z","timestamp":1760164825000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8380"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,4]]},"references-count":65,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["ijms22168380"],"URL":"https:\/\/doi.org\/10.3390\/ijms22168380","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,4]]}}}