{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T12:06:41Z","timestamp":1786709201425,"version":"build-2736575974"},"reference-count":50,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T00:00:00Z","timestamp":1628208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005632","name":"Narodowe Centrum Bada\u0144 i Rozwoju","doi-asserted-by":"publisher","award":["STRATEGMED2\/269807\/14\/NCBR\/2015"],"award-info":[{"award-number":["STRATEGMED2\/269807\/14\/NCBR\/2015"]}],"id":[{"id":"10.13039\/501100005632","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010665","name":"H2020 Marie Sk\u0142odowska-Curie Actions","doi-asserted-by":"publisher","award":["754432"],"award-info":[{"award-number":["754432"]}],"id":[{"id":"10.13039\/100010665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004569","name":"Ministerstwo Nauki i Szkolnictwa Wy\u017cszego","doi-asserted-by":"publisher","award":["2018\u20132023 co-financed project"],"award-info":[{"award-number":["2018\u20132023 co-financed project"]}],"id":[{"id":"10.13039\/501100004569","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Gamma rays and electrons with kinetic energy up to 10 MeV are routinely used to sterilize biomaterials. To date, the effects of irradiation upon human acellular dermal matrices (hADMs) remain to be fully elucidated. The optimal irradiation dosage remains a critical parameter affecting the final product structure and, by extension, its therapeutic potential. ADM slides were prepared by various digestion methods. The influence of various doses of radiation sterilization using a high-energy electron beam on the structure of collagen, the formation of free radicals and immune responses to non-irradiated (native) and irradiated hADM was investigated. The study of the structure changes was carried out using the following methods: immunohistology, immunoblotting, and electron paramagnetic resonance (EPR) spectroscopy. It was shown that radiation sterilization did not change the architecture and three-dimensional structure of hADM; however, it significantly influenced the degradation of collagen fibers and induced the production of free radicals in a dose-dependent manner. More importantly, the observed effects did not disrupt the therapeutic potential of the new transplants. Therefore, radiation sterilization at a dose of 35kGy can ensure high sterility of the dressing while maintaining its therapeutic potential.<\/jats:p>","DOI":"10.3390\/ijms22168467","type":"journal-article","created":{"date-parts":[[2021,8,6]],"date-time":"2021-08-06T08:01:42Z","timestamp":1628236902000},"page":"8467","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Optimization of Novel Human Acellular Dermal Dressing Sterilization for Routine Use in Clinical Practice"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1960-1071","authenticated-orcid":false,"given":"Hanna","family":"Lewandowska","sequence":"first","affiliation":[{"name":"Division of Radiation Modified Polymers, Centre for Radiation Research and Technology Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8980-1474","authenticated-orcid":false,"given":"Andrzej","family":"Eljaszewicz","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Immune Regulation, Medical University of Bia\u0142ystok, Waszyngtona 13, 15-269 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6007-5990","authenticated-orcid":false,"given":"Izabela","family":"Poplawska","sequence":"additional","affiliation":[{"name":"Department of Medical Pathomorphology, Medical University of Bia\u0142ystok, 15-230 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1693-7549","authenticated-orcid":false,"given":"Marlena","family":"Tynecka","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Immune Regulation, Medical University 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Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0902-1660","authenticated-orcid":false,"given":"Jordan","family":"Holl","sequence":"additional","affiliation":[{"name":"Department of Regenerative Medicine and Immune Regulation, Medical University of Bia\u0142ystok, Waszyngtona 13, 15-269 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0838-3478","authenticated-orcid":false,"given":"Hady","family":"Razak Hady","sequence":"additional","affiliation":[{"name":"Department of General and Endocrinological Surgery, Medical University of Bia\u0142ystok, 15-276 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Slawomir Lech","family":"Czaban","sequence":"additional","affiliation":[{"name":"Department of Anaesthesiology and Intensive Therapy, Faculty of Health Sciences, Medical University of Bia\u0142ystok, 15-274 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Reszec","sequence":"additional","affiliation":[{"name":"Department of Medical Pathomorphology, Medical University of Bia\u0142ystok, 15-230 Bia\u0142ystok, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gra\u017cyna","family":"Przybytniak","sequence":"additional","affiliation":[{"name":"Division of Radiation Modified Polymers, Centre for Radiation Research and Technology Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0825-5580","authenticated-orcid":false,"given":"Wojciech","family":"G\u0142uszewski","sequence":"additional","affiliation":[{"name":"Division of Radiation Modified Polymers, Centre for Radiation Research and Technology Institute of Nuclear Chemistry and Technology 16 Dorodna St., 03-195 Warsaw, 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