{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T05:51:16Z","timestamp":1762321876864,"version":"build-2065373602"},"reference-count":25,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T00:00:00Z","timestamp":1628640000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Recently, another new cell type was found in the perivascular space called a novel desmin-immunopositive perivascular (DIP) cell. However, the differences between this novel cell type and other nonhormone-producing cells have not been clarified. Therefore, we introduced several microscopic techniques to gain insight into the morphological characteristics of this novel DIP cell. We succeeded in identifying novel DIP cells under light microscopy using desmin immunocryosection, combining resin embedding blocks and immunoelectron microscopy. In conventional transmission electron microscopy, folliculostellate cells, capsular fibroblasts, macrophages, and pericytes presented a flat cisternae of rough endoplasmic reticulum, whereas those of novel DIP cells had a dilated pattern. The number of novel DIP cells was greatest in the intact rats, though nearly disappeared under prolactinoma conditions. Additionally, focused ion beam scanning electron microscopy showed that these novel DIP cells had multidirectional processes and some processes reached the capillary, but these processes did not tightly wrap the vessel, as is the case with pericytes. Interestingly, we found that the rough endoplasmic reticulum was globular and dispersed throughout the cytoplasmic processes after three-dimensional reconstruction. This study clearly confirms that novel DIP cells are a new cell type in the rat anterior pituitary gland, with unique characteristics.<\/jats:p>","DOI":"10.3390\/ijms22168630","type":"journal-article","created":{"date-parts":[[2021,8,11]],"date-time":"2021-08-11T08:35:52Z","timestamp":1628670952000},"page":"8630","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Insight into the Characteristics of Novel Desmin-Immunopositive Perivascular Cells of the Anterior Pituitary Gland Using Transmission and Focused Ion Beam Scanning Electron Microscopy"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9825-2692","authenticated-orcid":false,"given":"Depicha","family":"Jindatip","sequence":"first","affiliation":[{"name":"Department of Anatomy, Faculty of Medicine, Chulalongkorn University, 1873 Rama 4 Rd., Wangmai, Pathumwan, Bangkok 10330, Thailand"},{"name":"Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University, School of Medicine, 3311-1 Yakushiji, Shimotsuke 329-0498, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca Wan-Yan","family":"Poh","sequence":"additional","affiliation":[{"name":"Microscopy Business Group, Carl Zeiss Pte. Ltd., 50 Kaki Bukit Place, 05-01, Singapore 415926, Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ken","family":"Fujiwara","sequence":"additional","affiliation":[{"name":"Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University, School of Medicine, 3311-1 Yakushiji, Shimotsuke 329-0498, Japan"},{"name":"Department of Biological Sciences, Faculty of Science, Shonan Hiratsuka Campus, Kanagawa University, 2946 Tsuchiya Hiratsuka, Hiratsuka 259-1293, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1002\/ar.1092170408","article-title":"Appearance of the cyst- or ductule-like structures and their role in the restoration of the rat pituitary autograft","volume":"217","author":"Gon","year":"1987","journal-title":"Anat. Rec."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1002\/ar.1092240410","article-title":"Intercellular communication between rat anterior pituitary cells","volume":"224","author":"Soji","year":"1989","journal-title":"Anat. Rec."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s12565-012-0144-z","article-title":"Transmission and scanning electron microscopy study of the characteristics and morphology of pericytes and novel desmin-immunopositive perivascular cells before and after castration in rat anterior pituitary gland","volume":"87","author":"Jindatip","year":"2012","journal-title":"Anat. Sci. Int."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1021","DOI":"10.1038\/nmeth.3623","article-title":"Focused ion beams in biology","volume":"12","author":"Narayan","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1007\/978-1-62703-776-1_27","article-title":"Three-dimensional imaging of adherent cells using FIB\/SEM and STEM","volume":"1117","author":"Villinger","year":"2013","journal-title":"Methods Mol. Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"60","DOI":"10.3389\/fnana.2015.00060","article-title":"FIB\/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons","volume":"9","author":"Bosch","year":"2015","journal-title":"Front. Neuroanat."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1007\/s00429-017-1470-7","article-title":"Volume electron microscopy of the distribution of synapses in the neuropil of the juvenile rat somatosensory cortex","volume":"223","author":"Santuy","year":"2018","journal-title":"Brain Struct. Funct."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2535","DOI":"10.1002\/cne.24213","article-title":"Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB\/SEM","volume":"525","author":"Tamada","year":"2017","journal-title":"J. Comp. Neurol."},{"key":"ref_9","first-page":"21","article-title":"Two novel approaches to study arthropod anatomy by using dualbeam FIB\/SEM","volume":"106","author":"Muzzi","year":"2017","journal-title":"Micron"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"36019","DOI":"10.1038\/srep36019","article-title":"Three-dimensional ultrastructural analyses of anterior pituitary gland expose spatial relationships between endocrine cell secretory granule localization and capillary distribution","volume":"6","author":"Yoshitomi","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1007\/s12565-013-0180-3","article-title":"Changes in fine structure of pericytes and novel desmin-immunopositive perivascular cells during postnatal development in rat anterior pituitary gland","volume":"88","author":"Jindatip","year":"2013","journal-title":"Anat. Sci. Int."},{"key":"ref_12","first-page":"5610","article-title":"Human skeletal muscle fibroblasts, but not myogenic cells, readily undergo adipogenic differentiation","volume":"126","author":"Agley","year":"2013","journal-title":"J. Cell Sci."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1573","DOI":"10.1002\/dvdy.22280","article-title":"Cardiac fibroblast to myofibroblast differentiation in vivo and in vitro: Expression of focal adhesion components in neonatal and adult rat ventricular myofibroblasts","volume":"239","author":"Santiago","year":"2010","journal-title":"Dev. Dyn."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"267","DOI":"10.1016\/S0168-8278(88)80042-4","article-title":"Desmin distinguishes cultured fat-storing cells from myofibroblasts, smooth muscle cells and fibroblasts in the rat","volume":"6","author":"Takase","year":"1988","journal-title":"J. Hepatol."},{"key":"ref_15","first-page":"1433","article-title":"Adult stem cells and repair through granulation tissue","volume":"14","author":"Gutierrez","year":"2009","journal-title":"Front. Biosci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1111\/1523-1747.ep12514579","article-title":"Morphological characteristics of epithelial and fibroblastic cells growing out from biopsies of human skin","volume":"74","author":"Cassiman","year":"1980","journal-title":"J. Investig. Dermatol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1007\/s00441-010-1078-1","article-title":"In situ hybridization reveals that type I and III collagens are produced by pericytes in the anterior pituitary gland of rats","volume":"342","author":"Fujiwara","year":"2010","journal-title":"Cell Tissue Res."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1007\/s00441-012-1513-6","article-title":"Expression of small leucine-rich proteoglycans in rat anterior pituitary gland","volume":"351","author":"Horiguchi","year":"2013","journal-title":"Cell Tissue Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1530\/JOE-16-0033","article-title":"Folliculostellate cell interacts with pericyte via TGF\u03b22 in rat anterior pituitary","volume":"229","author":"Tsukada","year":"2016","journal-title":"J. Endocrinol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"61","DOI":"10.4161\/org.25144","article-title":"The importance of a single primary cilium","volume":"9","author":"Mahjoub","year":"2013","journal-title":"Organogenesis"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.ultramic.2007.10.010","article-title":"Imaging of intracellular spherical lamellar structures and tissue gross morphology by a focused ion beam\/scanning electron microscope (FIB\/SEM)","volume":"108","author":"Drobne","year":"2008","journal-title":"Ultramicroscopy"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1093\/jmicro\/dfv360","article-title":"Three-dimensional shape of the Golgi apparatus in different cell types: Serial section scanning electron microscopy of the osmium-impregnated Golgi apparatus","volume":"65","author":"Koga","year":"2016","journal-title":"Microscopy"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"278","DOI":"10.1016\/j.jsb.2013.11.008","article-title":"Multi-resolution correlative focused ion beam scanning electron microscopy: Applications to cell biology","volume":"185","author":"Narayan","year":"2014","journal-title":"J. Struct. Biol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"i34","DOI":"10.1093\/jmicro\/dfu076","article-title":"Three-dimensional organization of the endoplasmic reticulum membrane around the mitochondrial constriction site in mammalian cells revealed by using focused-ion beam tomography","volume":"63","author":"Ohta","year":"2014","journal-title":"Microscopy"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1007\/s00795-018-0180-4","article-title":"Characteristics of pericytes in diethylstilbestrol (DES)-induced pituitary prolactinoma in rats","volume":"51","author":"Jindatip","year":"2018","journal-title":"Med. Mol. Morphol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8630\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:44:00Z","timestamp":1760165040000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,11]]},"references-count":25,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["ijms22168630"],"URL":"https:\/\/doi.org\/10.3390\/ijms22168630","relation":{},"ISSN":["1422-0067"],"issn-type":[{"type":"electronic","value":"1422-0067"}],"subject":[],"published":{"date-parts":[[2021,8,11]]}}}