{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T19:23:39Z","timestamp":1792092219331,"version":"4.4.0"},"reference-count":52,"publisher":"MDPI AG","issue":"16","license":[{"start":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T00:00:00Z","timestamp":1629244800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004281","name":"Narodowe Centrum Nauki","doi-asserted-by":"publisher","award":["2016\/21\/N\/NZ1\/02850"],"award-info":[{"award-number":["2016\/21\/N\/NZ1\/02850"]}],"id":[{"id":"10.13039\/501100004281","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The Hfq protein is a bacterial RNA chaperone, involved in many molecular interactions, including control of actions of various small RNA regulatory molecules. We found that the presence of Hfq was required for survival of plasmid-containing Escherichia coli cells against high concentrations of chloramphenicol (plasmid p27cmr), tetracycline (pSC101, pBR322) and ampicillin (pBR322), as hfq+ strains were more resistant to these antibiotics than the hfq-null mutant. In striking contrast, production of Hfq resulted in low resistance to high concentrations of kanamycin when the antibiotic-resistance marker was chromosome-borne, with deletion of hfq resulting in increasing bacterial survival. These results were observed both in solid and liquid medium, suggesting that antibiotic resistance is an intrinsic feature of these strains rather than a consequence of adaptation. Despite its major role as RNA chaperone, which also affects mRNA stability, Hfq was not found to significantly affect kan and tet mRNAs turnover. Nevertheless, kan mRNA steady-state levels were higher in the hfq-null mutant compared to the hfq+ strain, suggesting that Hfq can act as a repressor of kan expression.This observation does correlate with the enhanced resistance to high levels of kanamycin observed in the hfq-null mutant. Furthermore, dependency on Hfq for resistance to high doses of tetracycline was found to depend on plasmid copy number, which was only observed when the resistance marker was expressed from a low copy plasmid (pSC101) but not from a medium copy plasmid (pBR322). This suggests that Hfq may influence survival against high doses of antibiotics through mechanisms that remain to be determined. Studies with pBR322\u0394rom may also suggest an interplay between Hfq and Rom in the regulation of ColE1-like plasmid replication. Results of experiments with a mutant devoid of the part of the hfq gene coding for the C-terminal region of Hfq suggested that this region, as well as the N-terminal region, may be involved in the regulation of expression of antibiotic resistance in E. coli independently.<\/jats:p>","DOI":"10.3390\/ijms22168886","type":"journal-article","created":{"date-parts":[[2021,8,18]],"date-time":"2021-08-18T10:54:35Z","timestamp":1629284075000},"page":"8886","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Differential Chromosome- and Plasmid-Borne Resistance of Escherichia coli hfq Mutants to High Concentrations of Various Antibiotics"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0820-7204","authenticated-orcid":false,"given":"Lidia","family":"Gaffke","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krzysztof","family":"Kubiak","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuzanna","family":"Cyske","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4042-7466","authenticated-orcid":false,"given":"Grzegorz","family":"W\u0119grzyn","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, University of Gdansk, Wita Stwosza 59, 80-308 Gdansk, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1016\/S0021-9258(19)45681-0","article-title":"Bacterial proteins required for replication of phage Q\u03b2 ribonucleic acid. Purification and properties of host factor I, a ribonucleic acid-binding protein","volume":"247","author":"Hayward","year":"1972","journal-title":"J. Biol. Chem."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1261\/rna.050047.115","article-title":"RNA reflections: Converging on Hfq","volume":"21","author":"Gottesman","year":"2015","journal-title":"RNA"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1016\/j.mib.2017.10.014","article-title":"New aspects of RNA-based regulation by Hfq and its partner sRNAs","volume":"42","author":"Kavita","year":"2018","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1007\/s00294-019-00990-y","article-title":"New molecular interactions broaden the functions of the RNA chaperone Hfq","volume":"65","author":"Arraiano","year":"2019","journal-title":"Curr. Genet."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"36","DOI":"10.3389\/fmolb.2016.00036","article-title":"The Escherichia coli Hfq protein: An unattended DNA-transactions regulator","volume":"3","author":"Cech","year":"2016","journal-title":"Front. Mol. Biosci."},{"key":"ref_6","first-page":"E6089","article-title":"C-terminal domain of the RNA chaperone Hfq drives sRNA competition and release of target RNA","volume":"113","author":"Kavita","year":"2016","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"10978","DOI":"10.1073\/pnas.1814428116","article-title":"Caulobacter crescentus Hfq structure reveals a conserved mechanism of RNA annealing regulation","volume":"116","author":"Jeliazkov","year":"2019","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"e00190","DOI":"10.1042\/BSR20140128","article-title":"New insight into the structure and function of Hfq C-terminus","volume":"35","author":"Fortas","year":"2015","journal-title":"Biosci. Rep."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"7299","DOI":"10.1093\/nar\/gkx431","article-title":"Compaction and condensation of DNA mediated by the C-terminal domain of Hfq","volume":"45","author":"Malabirade","year":"2017","journal-title":"Nucleic Acids Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"16792","DOI":"10.1038\/s41598-018-35060-9","article-title":"Revised role for Hfq bacterial regulator on DNA topology","volume":"8","author":"Malabirade","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"10","DOI":"10.1016\/j.plasmid.2014.04.005","article-title":"Hfq protein deficiency in Escherichia coli affects ColE1-like but not lambda plasmid DNA replication","volume":"73","author":"Cech","year":"2014","journal-title":"Plasmid"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Wien, F., Martinez, D., Le Brun, E., Jones, N.C., Vr\u00f8nning Hoffmann, S., Waeytens, J., Berbon, M., Habenstein, B., and Arluison, V. (2019). The bacterial amyloid-like Hfq promotes in vitro DNA alignment. Microorganisms, 7.","DOI":"10.3390\/microorganisms7120639"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Parekh, V.J., Niccum, B.A., Shah, R., Rivera, M.A., Novak, M.J., Geinguenaud, F., Wien, F., Arluison, V., and Sinden, R.R. (2020). Role of Hfq in genome evolution: Instability of G-quadruplex sequences in E. coli. Microorganisms, 8.","DOI":"10.3390\/microorganisms8010028"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"3668","DOI":"10.1021\/acs.biomac.0c00747","article-title":"Interactions between DNA and the Hfq amyloid-like region trigger a viscoelastic response","volume":"21","author":"Yadav","year":"2020","journal-title":"Biomacromolecules"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Parekh, V.J., Wien, F., Grange, W., De Long, T.A., Arluison, V., and Sinden, R.R. (2020). Crucial role of the C-terminal domain of Hfq protein in genomic instability. Microorganisms, 8.","DOI":"10.3390\/microorganisms8101598"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Geng, J., Song, Y., Yang, L., Feng, Y., Qiu, Y., Li, G., Guo, J., Bi, Y., Qu, Y., and Wang, W. (2009). Involvement of the post-transcriptional regulator Hfq in Yersinia pestis virulence. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006213"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"108722","DOI":"10.1016\/j.celrep.2021.108722","article-title":"MAPS integrates regulation of actin-targeting effector SteC into the virulence control network of Salmonella small RNA PinT","volume":"34","author":"Bischler","year":"2021","journal-title":"Cell Rep."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"610036","DOI":"10.3389\/fmicb.2020.610036","article-title":"AtxA-controlled small RNAs of Bacillus anthracis virulence plasmid pXO1 regulate gene xxpression in trans","volume":"11","author":"Corsi","year":"2021","journal-title":"Front. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"ftaa066","DOI":"10.1093\/femspd\/ftaa066","article-title":"Serovar-dependent differences in Hfq-regulated phenotypes in Actinobacillus pleuropneumoniae","volume":"78","author":"Crispim","year":"2020","journal-title":"Pathog. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dienstbier, A., Amman, F., \u0160tipl, D., Petr\u00e1\u010dkov\u00e1, D., and Ve\u010derek, B. (2019). Comparative integrated omics analysis of the Hfq regulon in Bordetella pertussis. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20123073"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"103559","DOI":"10.1016\/j.jprot.2019.103559","article-title":"Hfq modulates global protein pattern and stress response in Bordetella pertussis","volume":"211","author":"Surmann","year":"2020","journal-title":"J. Proteomics"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"e00232","DOI":"10.1128\/JB.00232-19","article-title":"Loss of RNA chaperone Hfq unveils a toxic pathway in Pseudomonas aeruginosa","volume":"201","author":"Hill","year":"2019","journal-title":"J. Bacteriol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.micres.2018.07.001","article-title":"An essential role for hfq involved in biofilm formation and virulence in serotype 4b Listeria monocytogenes","volume":"215","author":"Yao","year":"2018","journal-title":"Microbiol. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1093\/jac\/dkq067","article-title":"Impact of the RNA chaperone Hfq on multidrug resistance in Escherichia coli","volume":"65","author":"Yamada","year":"2010","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1659","DOI":"10.1093\/jac\/dkv042","article-title":"Systematic analysis of the role of bacterial Hfq-interacting sRNAs in the response to antibiotics","volume":"70","author":"Kim","year":"2015","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"803","DOI":"10.3389\/fmicb.2017.00803","article-title":"Roles of regulatory RNAs for antibiotic resistance in bacteria and their potential value as novel drug targets","volume":"8","author":"Dersch","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.micpath.2019.04.045","article-title":"RNA chaperone hfq mediates persistence to multiple antibiotics in Aeromonas veronii","volume":"132","author":"Zhang","year":"2019","journal-title":"Microb. Pathog."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"2709","DOI":"10.3389\/fmicb.2018.02709","article-title":"Harnessing metabolic regulation to increase Hfq-dependent antibiotic susceptibility in Pseudomonas aeruginosa","volume":"9","author":"Pusic","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e97631","DOI":"10.15252\/embj.201797631","article-title":"The RNA-binding protein Hfq is important for ribosome biogenesis and affects translation fidelity","volume":"37","author":"Andrade","year":"2018","journal-title":"EMBO J."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Turbant, F., Partouche, D., El Hamoui, O., Tr\u00e9pout, S., Legoubey, T., Wien, F., and Arluison, V. (2021). Apomorphine targets the pleiotropic bacterial regulator Hfq. Antibiotics, 10.","DOI":"10.3390\/antibiotics10030257"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"320","DOI":"10.1038\/nrmicro.2016.34","article-title":"Distinguishing between resistance, tolerance and persistence to antibiotic treatment","volume":"14","author":"Brauner","year":"2016","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"PLAS-0001-2013","DOI":"10.1128\/microbiolspec.PLAS-0001-2013","article-title":"Plasmid replication control by antisense RNAs","volume":"2","author":"Brantl","year":"2014","journal-title":"Microbiol. Spectr."},{"key":"ref_33","first-page":"PLAS-0029-2014","article-title":"Mechanisms of theta plasmid replication","volume":"3","author":"Lilly","year":"2015","journal-title":"Microbio. Spectr."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1186\/s13100-014-0027-z","article-title":"Tn5 transposition in Escherichia coli is repressed by Hfq and activated by over-expression of the small non-coding RNA SgrS","volume":"5","author":"Ross","year":"2014","journal-title":"Mob. DNA"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/mmi.12961","article-title":"Hfq binds directly to the ribosome-binding site of IS10 transposase mRNA to inhibit translation","volume":"96","author":"Ellis","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Le Derout, J., Boni, I.V., R\u00e9gnier, P., and Hajnsdorf, E. (2010). Hfq affects mRNA levels independently of degradation. BMC Mol. Biol., 11.","DOI":"10.1186\/1471-2199-11-17"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1080\/15476286.2019.1672514","article-title":"Mechanism for coordinate regulation of rpoS by sRNA-sRNA interaction in Escherichia coli","volume":"17","author":"Kim","year":"2020","journal-title":"RNA Biol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3309","DOI":"10.1111\/1462-2920.13052","article-title":"The RNA chaperone Hfq enables the environmental stress tolerance super-phenotype of Pseudomonas putida","volume":"18","author":"Calles","year":"2016","journal-title":"Environ. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2400","DOI":"10.1093\/nar\/gkv072","article-title":"Structural insights into the recognition of the internal A-rich linker from OxyS sRNA by Escherichia coli Hfq","volume":"43","author":"Wang","year":"2015","journal-title":"Nucleic Acids Res."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1016\/j.jmb.2013.08.026","article-title":"Positional effects of AAN motifs in rpoS regulation by sRNAs and Hfq","volume":"426","author":"Peng","year":"2014","journal-title":"J. Mol. Biol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"5938","DOI":"10.1093\/nar\/gkt276","article-title":"Hfq-bridged ternary complex is important for translation activation of rpoS by DsrA","volume":"41","author":"Wang","year":"2013","journal-title":"Nucleic Acids Res."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"126804","DOI":"10.1016\/j.micres.2021.126804","article-title":"Stress preadaptation and overexpression of rpoS and hfq genes increase stress resistance of Pseudomonas fluorescens ATCC13525","volume":"250","author":"Wu","year":"2021","journal-title":"Microbiol. Res."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"3401","DOI":"10.1128\/jb.175.11.3401-3407.1993","article-title":"The Escherichia coli K-12 \u201cwild types\u201d W3110 and MG1655 have an rph frameshift mutation that leads to pyrimidine starvation due to low pyrE expression levels","volume":"175","author":"Jensen","year":"1993","journal-title":"J. Bacteriol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"88","DOI":"10.2144\/01311st05","article-title":"Integration of PCR fragments at any specific site within cloning vectors without the use of restriction enzymes and DNA ligase","volume":"31","author":"Geiser","year":"2001","journal-title":"Biotechniques"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"6640","DOI":"10.1073\/pnas.120163297","article-title":"One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products","volume":"97","author":"Datsenko","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_46","first-page":"211","article-title":"Plasmids derived from lambdoid bacteriophages as models for studying replication of mobile genetic elements responsible for the production of Shiga toxins by pathogenic Escherichia coli strains","volume":"17","author":"Nejman","year":"2009","journal-title":"J. Mol. Microbiol. Biotechnol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/mr.53.4.491-516.1989","article-title":"Replication of plasmids in gramnegative bacteria","volume":"53","author":"Kues","year":"1989","journal-title":"Microbiol. Rev."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"95","DOI":"10.1016\/0378-1119(77)90000-2","article-title":"Construction and characterization of new cloning vehicles. II. A multipurpose cloning system","volume":"2","author":"Bolivar","year":"1977","journal-title":"Gene"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"4121","DOI":"10.1128\/jb.177.14.4121-4130.1995","article-title":"Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter","volume":"177","author":"Guzman","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_50","unstructured":"Sambrook, J., Fritsch, E.F., and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory, Press."},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Bloch, S., Nejman-Fale\u0144czyk, B., Dydecka, A., \u0141o\u015b, J.M., Felczykowska, A., W\u0119grzyn, A., and W\u0119grzyn, G. (2014). Different expression patterns of genes from the exo-xis region of bacteriophage \u03bb and Shiga toxin-converting bacteriophage \u03a624B following infection or prophage induction in Escherichia coli. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0108233"},{"key":"ref_52","unstructured":"Roche (2009). LightCycler Real-Time PCR Systems\u2014Application Manual, Roche Diagnostics GmbH."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8886\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T06:46:32Z","timestamp":1760165192000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/16\/8886"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,8,18]]},"references-count":52,"journal-issue":{"issue":"16","published-online":{"date-parts":[[2021,8]]}},"alternative-id":["ijms22168886"],"URL":"https:\/\/doi.org\/10.3390\/ijms22168886","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,8,18]]}}}