{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T14:27:50Z","timestamp":1788186470571,"version":"build-2803163510"},"reference-count":49,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T00:00:00Z","timestamp":1629936000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Horizon 2020","award":["H2020-SC1-2016-RTD\/731944"],"award-info":[{"award-number":["H2020-SC1-2016-RTD\/731944"]}]},{"DOI":"10.13039\/501100002661","name":"Fonds De La Recherche Scientifique - FNRS","doi-asserted-by":"publisher","award":["J.0018.18"],"award-info":[{"award-number":["J.0018.18"]}],"id":[{"id":"10.13039\/501100002661","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["EY013462"],"award-info":[{"award-number":["EY013462"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Sj\u00f6gren\u2019s syndrome (SS) is an exocrinopathy characterized by the hypofunction of salivary glands (SGs). Aquaporin-5 (AQP5); a water channel involved in saliva formation; is aberrantly distributed in SS SG acini and contributes to glandular dysfunction. We aimed to investigate the role of ezrin in AQP5 mislocalization in SS SGs. The AQP5\u2013ezrin interaction was assessed by immunoprecipitation and proteome analysis and by proximity ligation assay in immortalized human SG cells. We demonstrated, for the first time, an interaction between ezrin and AQP5. A model of the complex was derived by computer modeling and in silico docking; suggesting that AQP5 interacts with the ezrin FERM-domain via its C-terminus. The interaction was also investigated in human minor salivary gland (hMSG) acini from SS patients (SICCA-SS); showing that AQP5\u2013ezrin complexes were absent or mislocalized to the basolateral side of SG acini rather than the apical region compared to controls (SICCA-NS). Furthermore, in SICCA-SS hMSG acinar cells, ezrin immunoreactivity was decreased at the acinar apical region and higher at basal or lateral regions, accounting for altered AQP5\u2013ezrin co-localization. Our data reveal that AQP5\u2013ezrin interactions in human SGs could be involved in the regulation of AQP5 trafficking and may contribute to AQP5-altered localization in SS patients<\/jats:p>","DOI":"10.3390\/ijms22179213","type":"journal-article","created":{"date-parts":[[2021,8,26]],"date-time":"2021-08-26T09:27:57Z","timestamp":1629970077000},"page":"9213","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Ezrin Is a Novel Protein Partner of Aquaporin-5 in Human Salivary Glands and Shows Altered Expression and Cellular Localization in Sj\u00f6gren\u2019s Syndrome"],"prefix":"10.3390","volume":"22","author":[{"given":"Clara","family":"Chivasso","sequence":"first","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2088-7281","authenticated-orcid":false,"given":"Carl Johan","family":"Hagstr\u00f6mer","sequence":"additional","affiliation":[{"name":"Division of Biochemistry and Structural Biology, Lund University, 221 00 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristie L.","family":"Rose","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37240, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Florent","family":"Lhotellerie","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lionel","family":"Leblanc","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37240, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9283-6902","authenticated-orcid":false,"given":"Stefania","family":"Moscato","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cl\u00e9ment","family":"Chevalier","sequence":"additional","affiliation":[{"name":"Center for Microscopy and Molecular Imaging (CMMI), 6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egor","family":"Zindy","sequence":"additional","affiliation":[{"name":"Center for Microscopy and Molecular Imaging (CMMI), 6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maud","family":"Martin","sequence":"additional","affiliation":[{"name":"Laboratory of Neurovascular Signaling, Universit\u00e9 Libre de Bruxelles, 6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benoit","family":"Vanhollebeke","sequence":"additional","affiliation":[{"name":"Laboratory of Neurovascular Signaling, Universit\u00e9 Libre de Bruxelles, 6041 Gosselies, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7oise","family":"Gregoire","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nargis","family":"Bolaky","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7161-7604","authenticated-orcid":false,"given":"Jason","family":"Perret","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Baldini","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Shahnawaz","family":"Soyfoo","sequence":"additional","affiliation":[{"name":"Department of Rheumatology, Erasme Hospital, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6835-7987","authenticated-orcid":false,"given":"Letizia","family":"Mattii","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine, University of Pisa, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin L.","family":"Schey","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN 37240, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9435-8243","authenticated-orcid":false,"given":"Susanna","family":"T\u00f6rnroth-Horsefield","sequence":"additional","affiliation":[{"name":"Division of Biochemistry and Structural Biology, Lund University, 221 00 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0385-5824","authenticated-orcid":false,"given":"Christine","family":"Delporte","sequence":"additional","affiliation":[{"name":"Laboratory of Pathophysiological and Nutritional Biochemistry, Universit\u00e9 Libre de Bruxelles, 1070 Brussels, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"4278","DOI":"10.1002\/anie.200460804","article-title":"Aquaporin water channels (Nobel Lecture)","volume":"43","author":"Agre","year":"2004","journal-title":"Angew. 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