{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:28:42Z","timestamp":1787592522975,"version":"build-2736575974"},"reference-count":25,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2021,8,29]],"date-time":"2021-08-29T00:00:00Z","timestamp":1630195200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Foundation Q17 of Korea (NRF) grant funded by the Korean Government\u2019s Ministry of Science, ICT and Future Planning","award":["2020R1I1A3A04036500"],"award-info":[{"award-number":["2020R1I1A3A04036500"]}]},{"name":"a grant from the Korea Health Technology R&amp;D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health and Welfare, South Korea","award":["HF20C0019"],"award-info":[{"award-number":["HF20C0019"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Parkinson\u2019s disease (PD) is characterized by a loss of dopaminergic cells in the substantia nigra, and its histopathological features include the presence of fibrillar aggregates of \u03b1-synuclein (\u03b1-syn), which are called Lewy bodies and Lewy neurites. Lewy pathology has been identified not only in the brain but also in various tissues, including muscles. This study aimed to investigate the link between serine\/arginine-rich protein specific kinase 3 (srpk3) and \u03b1-syn in muscles in PD. We conducted experiments on the quadriceps femoris of a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced PD mouse model and the C2C12 cell line after treatment with 1-methyl-4-phenylpyridinium (MPP+) and srpk3 short interfering RNA (siRNA). Compared to the control group, the MPTP group showed significantly reduced expression of srpk3, but increased expression of \u03b1-syn. In MPP+-treated C2C12 cells, srpk3 expression gradually decreased and \u03b1-syn expression increased with the increasing MPP+ concentration. Moreover, experiments in C2C12 cells using srpk3 siRNA showed increased expressions of \u03b1-syn and phosphorylated \u03b1-syn. Our results showed that srpk3 expression could be altered by MPTP intoxication in muscles, and this change may be related to changes in \u03b1-syn expression. Furthermore, this study could contribute to advancement of research on the mechanism by which srpk3 plays a role in PD.<\/jats:p>","DOI":"10.3390\/ijms22179375","type":"journal-article","created":{"date-parts":[[2021,8,29]],"date-time":"2021-08-29T21:45:16Z","timestamp":1630273516000},"page":"9375","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Srpk3 Decrease Associated with Alpha-Synuclein Increase in Muscles of MPTP-Induced Parkinson\u2019s Disease Mice"],"prefix":"10.3390","volume":"22","author":[{"given":"Min Hyung","family":"Seo","sequence":"first","affiliation":[{"name":"Department of Meridian and Acupoint, College of Korean Medicine, Sang Ji University, Wonju 26339, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9883-6153","authenticated-orcid":false,"given":"Sujung","family":"Yeo","sequence":"additional","affiliation":[{"name":"Department of Meridian and Acupoint, College of Korean Medicine, Sang Ji University, Wonju 26339, Korea"},{"name":"Research Institute of Korean Medicine, Sang Ji University, Wonju 26339, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,29]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1093\/brain\/124.11.2131","article-title":"Pathophysiology of bradykinesia in Parkinson\u2019s disease","volume":"124","author":"Berardelli","year":"2001","journal-title":"Brain"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1007\/BF00294234","article-title":"Parkinson\u2019s disease: An immunohistochemical study of Lewy body-containing neurons in the enteric nervous system","volume":"79","author":"Wakabayashi","year":"1990","journal-title":"Acta Neuropathol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/j.neulet.2005.11.012","article-title":"Gastric \u03b1-synuclein immunoreactive inclusions in Meissner\u2019s and Auerbach\u2019s plexuses in cases staged for Parkinson\u2019s disease-related brain pathology","volume":"396","author":"Braak","year":"2006","journal-title":"Neurosci. 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