{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T02:19:44Z","timestamp":1760235584802,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T00:00:00Z","timestamp":1630281600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002332","name":"Semmelweis Egyetem","doi-asserted-by":"publisher","award":["OTKA PD_132851","OTKA K_125174"],"award-info":[{"award-number":["OTKA PD_132851","OTKA K_125174"]}],"id":[{"id":"10.13039\/501100002332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>We examined the vasoactive effect of estradiol in a rat model of early PCOS and the influence of vitamin D deficiency (VDD). We created a model of chronic hyperandrogenism and VDD in adolescent female Wistar rats (N = 46) with four experimental groups: vitamin D supplemented (T-D+), VDD (T-D-), hyperandrogenic and vitamin D supplemented (T+D+), and hyperandrogenic and VDD (T+D-). T+ groups received an 8-week-long transdermal Androgel treatment, D-animals were on vitamin D-reduced diet and D+ rats were supplemented orally with vitamin D3. Estrogen-induced vasorelaxation of thoracic aorta segments were measured with a wire myograph system with or without the inhibition of endothelial nitric oxide synthase (eNOS) or cyclooxygenase-2 (COX-2). The distribution of estrogen receptor (ER), eNOS and COX-2 in the aortic wall was assessed by immunohistochemistry. VDD aortas showed significantly lower estradiol-induced relaxation independently of androgenic status that was further decreased by COX-2 inhibition. COX-2 inhibition failed to alter vessel function in D+ rats. Inhibition of eNOS abolished the estradiol-induced relaxation in all groups. Changes in vascular function in VDD were accompanied by significantly decreased ER and eNOS staining. Short-term chronic hyperandrogenism failed to, but VDD induced vascular dysfunction, compromised estrogen-dependent vasodilatation and changes in ER and eNOS immunostaining.<\/jats:p>","DOI":"10.3390\/ijms22179404","type":"journal-article","created":{"date-parts":[[2021,8,30]],"date-time":"2021-08-30T11:01:37Z","timestamp":1630321297000},"page":"9404","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Influence of Vitamin D on the Vasoactive Effect of Estradiol in a Rat Model of Polycystic Ovary Syndrome"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3837-8020","authenticated-orcid":false,"given":"R\u00f3bert","family":"Tarszab\u00f3","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology, Markusovszky Lajos University Teaching Hospital, Markusovszky Lajos Street 5, 9700 Szombathely, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5531-0661","authenticated-orcid":false,"given":"B\u00e1lint","family":"B\u00e1nyai","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c9va","family":"Ruisanchez","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Borb\u00e1la","family":"P\u00e9terffy","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1gnes","family":"Kors\u00f3s-Nov\u00e1k","sequence":"additional","affiliation":[{"name":"Department of Pathology, Het\u00e9nyi G\u00e9za Hospital, T\u00f3szegi Street 21, 5000 Szolnok, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6731-3309","authenticated-orcid":false,"given":"Krisztina","family":"Lajtai","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"},{"name":"Department of Obstetrics and Gynecology, Semmelweis University, \u00dcll\u0151i Street 78\/a, 1082 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-4331","authenticated-orcid":false,"given":"R\u00e9ka Eszter","family":"Sziva","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"},{"name":"Department of Obstetrics and Gynecology, Semmelweis University, \u00dcll\u0151i Street 78\/a, 1082 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D\u00f3ra","family":"Gerszi","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"},{"name":"Department of Obstetrics and Gynecology, Semmelweis University, \u00dcll\u0151i Street 78\/a, 1082 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00c1d\u00e1m","family":"Hossz\u00fa","sequence":"additional","affiliation":[{"name":"1st Department of Pediatrics, Semmelweis University, 1082 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7740-9128","authenticated-orcid":false,"given":"Rita","family":"Benk\u0151","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6015-0359","authenticated-orcid":false,"given":"Zolt\u00e1n","family":"Beny\u00f3","sequence":"additional","affiliation":[{"name":"Department of Translational Medicine, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eszter M\u00e1ria","family":"Horv\u00e1th","sequence":"additional","affiliation":[{"name":"Department of Physiology, Semmelweis University, T\u0171zolt\u00f3 Street 37-47, 1094 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella","family":"Masszi","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, National Institute of Psychiatry and Addictions, Lehel Street 59-61, 1135 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Szabolcs","family":"V\u00e1rb\u00edr\u00f3","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, Semmelweis University, \u00dcll\u0151i Street 78\/a, 1082 Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,8,30]]},"reference":[{"key":"ref_1","first-page":"3078","article-title":"Prevalence of the polycystic ovary syndrome in unselected black and white women of the southeastern United States: A prospective study","volume":"83","author":"Knochenhauer","year":"1998","journal-title":"J. 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