{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:01:10Z","timestamp":1790812870311,"version":"4.1.0"},"reference-count":75,"publisher":"MDPI AG","issue":"17","license":[{"start":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T00:00:00Z","timestamp":1630540800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100014721","name":"Beneficientia Stiftung","doi-asserted-by":"publisher","award":["BEN2019\/48"],"award-info":[{"award-number":["BEN2019\/48"]}],"id":[{"id":"10.13039\/501100014721","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007514","name":"Universit\u00e0 di Pisa","doi-asserted-by":"publisher","award":["PRA - Progetti di Ricerca di Ateneo\u201d Institutional Research Grants - Project no. PRA_2020_58 \u201cAgenti innovativi e nanosistemi per target molecolari nell'ambito dell'oncologia di precisione"],"award-info":[{"award-number":["PRA - Progetti di Ricerca di Ateneo\u201d Institutional Research Grants - Project no. PRA_2020_58 \u201cAgenti innovativi e nanosistemi per target molecolari nell'ambito dell'oncologia di precisione"]}],"id":[{"id":"10.13039\/501100007514","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003407","name":"Ministero dell\u2019Istruzione, dell\u2019Universit\u00e0 e della Ricerca","doi-asserted-by":"publisher","award":["PRIN call, project code: 2017WBZFHL"],"award-info":[{"award-number":["PRIN call, project code: 2017WBZFHL"]}],"id":[{"id":"10.13039\/501100003407","id-type":"DOI","asserted-by":"publisher"}]},{"name":"M-ERA-NET 2","award":["\"SmartHyCAR\", number: 4274"],"award-info":[{"award-number":["\"SmartHyCAR\", number: 4274"]}]},{"DOI":"10.13039\/501100004505","name":"Universit\u00e0 di Catania","doi-asserted-by":"publisher","award":["PIAno di inCEntivi per la RIcerca di Ateneo 2020\/2022 CHANCE_Linea di Intervento 1"],"award-info":[{"award-number":["PIAno di inCEntivi per la RIcerca di Ateneo 2020\/2022 CHANCE_Linea di Intervento 1"]}],"id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004505","name":"Universit\u00e0 di Catania","doi-asserted-by":"publisher","award":["GRABIO_Linea di intervento 2"],"award-info":[{"award-number":["GRABIO_Linea di intervento 2"]}],"id":[{"id":"10.13039\/501100004505","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The angiogenin protein (ANG) is one of the most potent endogenous angiogenic factors. In this work we characterized by means of potentiometric, spectroscopic and voltammetric techniques, the copper complex species formed with peptide fragments derived from the N-terminal domain of the protein, encompassing the sequence 1-17 and having free amino, Ang1-17, or acetylated N-terminus group, AcAng1-17, so to explore the role of amino group in metal binding and cellular copper uptake. The obtained data show that amino group is the main copper anchoring site for Ang1-17. The affinity constant values, metal coordination geometry and complexes redox-potentials strongly depend, for both peptides, on the number of copper equivalents added. Confocal laser scanning microscope analysis on neuroblastoma cells showed that in the presence of one equivalent of copper ion, the free amino Ang1-17 increases cellular copper uptake while the acetylated AcAng1-17 strongly decreases the intracellular metal level. The activity of peptides was also compared to that of the protein normally present in the plasma (wtANG) as well as to the recombinant form (rANG) most commonly used in literature experiments. The two protein isoforms bind copper ions but with a different coordination environment. Confocal laser scanning microscope data showed that the wtANG induces a strong increase in intracellular copper compared to control while the rANG decreases the copper signal inside cells. These data demonstrate the relevance of copper complexes\u2019 geometry to modulate peptides\u2019 activity and show that wtANG, normally present in the plasma, can affect cellular copper uptake.<\/jats:p>","DOI":"10.3390\/ijms22179530","type":"journal-article","created":{"date-parts":[[2021,9,2]],"date-time":"2021-09-02T10:02:11Z","timestamp":1630576931000},"page":"9530","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Peptides Derived from Angiogenin Regulate Cellular Copper Uptake"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1990-3723","authenticated-orcid":false,"given":"Giovanni","family":"Tabb\u00ec","sequence":"first","affiliation":[{"name":"Institute of Crystallography\u2014National Council of Research\u2014CNR, via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6127-5962","authenticated-orcid":false,"given":"Lorena Maria","family":"Cucci","sequence":"additional","affiliation":[{"name":"Nano Hybrid BioInterfaces Lab (NHBIL), Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4290-0336","authenticated-orcid":false,"given":"Calogero","family":"Pinzino","sequence":"additional","affiliation":[{"name":"Institute for the Chemistry of OrganoMetallic Compounds (ICCOM), National Council of Research\u2014CNR, via G. Moruzzi 1, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessia","family":"Munzone","sequence":"additional","affiliation":[{"name":"Aix-Marseille Univesit\u00e9, 52 Avenue Escadrille Normandie Niemen, 13013 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2567-3637","authenticated-orcid":false,"given":"Tiziano","family":"Marzo","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, via Bonanno Pisano 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1150-1551","authenticated-orcid":false,"given":"Silvia","family":"Pizzanelli","sequence":"additional","affiliation":[{"name":"Institute for the Chemistry of OrganoMetallic Compounds (ICCOM), National Council of Research\u2014CNR, via G. Moruzzi 1, 56124 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5348-5863","authenticated-orcid":false,"given":"Cristina","family":"Satriano","sequence":"additional","affiliation":[{"name":"Nano Hybrid BioInterfaces Lab (NHBIL), Department of Chemical Sciences, University of Catania, Viale Andrea Doria 6, 95125 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1556-934X","authenticated-orcid":false,"given":"Antonio","family":"Magr\u00ec","sequence":"additional","affiliation":[{"name":"Institute of Crystallography\u2014National Council of Research\u2014CNR, via Paolo Gaifami 18, 95126 Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4193-7612","authenticated-orcid":false,"given":"Diego","family":"La Mendola","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Pisa, via Bonanno Pisano 6, 56126 Pisa, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1080\/15476286.2016.1272746","article-title":"RNA biology of angiogenin: Current state and perspectives","volume":"14","author":"Lyons","year":"2016","journal-title":"RNA Biol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"399","DOI":"10.1093\/abbs\/gmv131","article-title":"Three decades of research on angiogenin: A review and perspective","volume":"48","author":"Sheng","year":"2016","journal-title":"Acta Biochim. 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