{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T12:02:23Z","timestamp":1778673743586,"version":"3.51.4"},"reference-count":54,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T00:00:00Z","timestamp":1631232000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003621","name":"Ministry of Science, ICT and Future Planning","doi-asserted-by":"publisher","award":["2014R1A2A1A11052187"],"award-info":[{"award-number":["2014R1A2A1A11052187"]}],"id":[{"id":"10.13039\/501100003621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Amino acids, as nutrients, are expected to improve sleep disorders. This study aimed to evaluate the generation- and age-dependent sleep-improving effects of \u03b3-aminobutyric acid (GABA) and 5-hydroxytryptophan (5-HTP) coadministration. The differentially expressed genes and generation-related behavior after the administration of a GABA\/5-HTP mixture were measured in a Drosophila model, while age-related changes in gene expression and oxidative stress-related parameters were measured in a mouse model. The GABA\/5-HTP-treated group showed significant behavioral changes compared to the other groups. Sequencing revealed that the GABA\/5-HTP mixture influenced changes in nervous system-related genes, including those involved in the regulation of the expression of behavioral and synaptic genes. Additionally, total sleep time increased with age, and nighttime sleep time in the first- and third-generation flies was significantly different from that of the control groups. The GABA\/5-HTP mixture induced significant changes in the expression of sleep-related receptors in both models. Furthermore, the GABA\/5-HTP mixture reduced levels of ROS and ROS reaction products in an age-dependent manner. Therefore, the increase in behavioral changes caused by GABA\/5-HTP mixture administration was effective in eliminating ROS activity across generations and ages.<\/jats:p>","DOI":"10.3390\/ijms22189822","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T21:45:57Z","timestamp":1631483157000},"page":"9822","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Changes in Locomotor Activity and Oxidative Stress-Related Factors after the Administration of an Amino Acid Mixture by Generation and Age"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7016-9798","authenticated-orcid":false,"given":"Yejin","family":"Ahn","sequence":"first","affiliation":[{"name":"Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5199-2685","authenticated-orcid":false,"given":"Ki-Bae","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Food Science and Nutrition, Jeju National University, Jeju 63243, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suhyeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea"},{"name":"Lotte R&D Center, Seoul 07594, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8869-3929","authenticated-orcid":false,"given":"Hyung Joo","family":"Suh","sequence":"additional","affiliation":[{"name":"Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea"},{"name":"Transdisciplinary Major in Learning Health Systems, Department of Healthcare Sciences, Graduate School, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4056-6706","authenticated-orcid":false,"given":"Kyungae","family":"Jo","sequence":"additional","affiliation":[{"name":"Department of Integrated Biomedical and Life Science, Graduate School, Korea University, Seoul 02841, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"149","DOI":"10.18071\/isz.71.0149","article-title":"Pharmacological and nonpharmacological treatment of insomnias with regard to sleep medicine","volume":"71","author":"Faludi","year":"2018","journal-title":"Ideggyogyaszati szemle"},{"key":"ref_2","first-page":"1","article-title":"Treating insomnia with medications","volume":"2","author":"Pagel","year":"2018","journal-title":"Sleep Sci. 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