{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T07:42:14Z","timestamp":1793691734452,"version":"4.5.1"},"reference-count":33,"publisher":"MDPI AG","issue":"18","license":[{"start":{"date-parts":[[2021,9,11]],"date-time":"2021-09-11T00:00:00Z","timestamp":1631318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale","doi-asserted-by":"publisher","award":["ATIP-Avenir 2019"],"award-info":[{"award-number":["ATIP-Avenir 2019"]}],"id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002738","name":"Assistance Publique - H\u00f4pitaux de Paris","doi-asserted-by":"publisher","award":["Ann\u00e9e Recherche"],"award-info":[{"award-number":["Ann\u00e9e Recherche"]}],"id":[{"id":"10.13039\/501100002738","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Ischemia is a common cause of acute kidney injury worldwide, frequently occurring in patients undergoing cardiac surgery or admitted to the intensive care unit (ICU). Thus, ischemia-reperfusion injury (IRI) remains one of the main experimental models for the study of kidney diseases. However, the classical technique, based on non-traumatic surgical clamps, suffers from several limitations. It does not allow the induction of multiple episodes of acute kidney injury (AKI) in the same animal, which would be relevant from a human perspective. It also requires a deep and long sedation, raising the question of potential anaesthesia-related biases. We designed a vascular occluding device that can be activated remotely in conscious mice. We first assessed the intensity and the reproducibility of the acute kidney injury induced by this new device. We finally investigated the role played by the anaesthesia in the IRI models at the histological, functional and transcriptomic levels. We showed that this technique allows the rapid induction of renal ischemia in a repeatable and reproducible manner, breaking several classical limitations. In addition, we used its unique specificities to highlight the renal protective effect conferred by the anaesthesia, related to the mitigation of the IRI transcriptomic program.<\/jats:p>","DOI":"10.3390\/ijms22189840","type":"journal-article","created":{"date-parts":[[2021,9,12]],"date-time":"2021-09-12T21:45:57Z","timestamp":1631483157000},"page":"9840","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Anaesthesia-Induced Transcriptomic Changes in the Context of Renal Ischemia Uncovered by the Use of a Novel Clamping Device"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8922-6038","authenticated-orcid":false,"given":"Charles","family":"Verney","sequence":"first","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Legouis","sequence":"additional","affiliation":[{"name":"Laboratory of Nephrology, Department of Medicine, University Hospitals of Geneva, 1205 Geneva, Switzerland"},{"name":"Department of Cell Physiology, Faculty of Medicine, University of Geneva, 1205 Geneva, Switzerland"},{"name":"Division of Intensive Care, University Hospital of Geneva, 1205 Geneva, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Placier","sequence":"additional","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiffany","family":"Migeon","sequence":"additional","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3184-3740","authenticated-orcid":false,"given":"Philippe","family":"Bonnin","sequence":"additional","affiliation":[{"name":"INSERM U1148 Laboratory for Vascular Translational Science (LVTS) H\u00f4pital Bichat, F-75018 Paris, France"},{"name":"Physiologie Clinique\u2014Explorations Fonctionnelles, Assistance Publique H\u00f4pitaux de Paris (APHP), Lariboisi\u00e8re Hospital, F-75010 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Buob","sequence":"additional","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"},{"name":"Department of Anatomopathology, AP-HP, Tenon Hospital, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8260-6265","authenticated-orcid":false,"given":"Juliette","family":"Hadchouel","sequence":"additional","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2771-1596","authenticated-orcid":false,"given":"Pierre","family":"Galichon","sequence":"additional","affiliation":[{"name":"Common and Rare Kidney Diseases (CoRaKID) Unit, Institut National de la Sant\u00e9 and de la Recherche M\u00e9dicale (INSERM) U1155, F-75020 Paris, France"},{"name":"CoRaKID Unit, Sorbonne Universit\u00e9, F-75020 Paris, France"},{"name":"Surgical and Medical Department of Kidney Transplantation, Assistance Publique\u2013H\u00f4pitaux de Paris (APHP), Piti\u00e9-Salp\u00eatri\u00e8re Hospital, F-75013 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1411","DOI":"10.1007\/s00134-015-3934-7","article-title":"Epidemiology of acute kidney injury in critically ill patients: The multinational AKI-EPI 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