{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T15:14:05Z","timestamp":1782227645366,"version":"3.54.5"},"reference-count":47,"publisher":"MDPI AG","issue":"19","license":[{"start":{"date-parts":[[2021,9,30]],"date-time":"2021-09-30T00:00:00Z","timestamp":1632960000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Russian Science Foundation grant","award":["20-14-00055"],"award-info":[{"award-number":["20-14-00055"]}]},{"name":"Budget grant","award":["0259-2021-0015"],"award-info":[{"award-number":["0259-2021-0015"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The latest vaccination campaign has actualized the potential impact of antigenic stimuli on reproductive functions. To address this, we mimicked vaccination\u2019s effects by administering keyhole limpet hemocyanin (KLH ) to CD1 male mice and used their sperm for in vitro fertilization (IVF). Two-cell embryos after IVF with spermatozoa from control (C) or KLH-treated (Im) male mice were transferred to surrogate mothers mated with vasectomized control (C) or KLH-treated (Im) male mice, resulting in four experimental groups: C\u2013C, Im\u2013C, C\u2013Im, and Im\u2013Im. The pre-implantation losses were significantly lower in the Im\u2013C group than in the C\u2013Im group. At the same time, the resorption rates reduced markedly in the C\u2013Im compared to the Im\u2013C group. Embryo and placenta weights were significantly higher in the Im\u2013Im group. Although the GM-CSF levels were lower in the amniotic fluid of the gestating surrogate mothers in the Im\u2013Im group, they were strongly correlated with embryo mass. The number\u2013size trade-off was only significant in the Im\u2013Im group. This suggests a positive, cooperative effect of spermatozoa and seminal fluid from immune-primed males on embryo growth and the optimal distribution of surrogate mother maternal resources despite the negative impact of males\u2019 antigenic challenge on the IVF success rate.<\/jats:p>","DOI":"10.3390\/ijms221910650","type":"journal-article","created":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T10:55:40Z","timestamp":1633085740000},"page":"10650","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Effects of Sperm and Seminal Fluid of Immunized Male Mice on In Vitro Fertilization and Surrogate Mother\u2013Embryo Interaction"],"prefix":"10.3390","volume":"22","author":[{"given":"Galina Vladimirovna","family":"Kontsevaya","sequence":"first","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8118-1362","authenticated-orcid":false,"given":"Ludmila Alekseevna","family":"Gerlinskaya","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yury Mikhailovich","family":"Moshkin","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita Vladimirovna","family":"Anisimova","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aliya Konstantinovna","family":"Stanova","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatyana Ivanovna","family":"Babochkina","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mikhail Pavlovich","family":"Moshkin","sequence":"additional","affiliation":[{"name":"Federal Research Center Institute of Cytology and Genetics, Siberian Branch of RAS, 630090 Novosibirsk, Russia"},{"name":"Biological Institute at Tomsk State University, 634050 Tomsk, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,9,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1016\/j.cmet.2016.01.014","article-title":"Paternal Psychological Stress Reprograms Hepatic Gluconeogenesis in Offspring","volume":"23","author":"Wu","year":"2016","journal-title":"Cell Metab."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1372","DOI":"10.1038\/s41591-018-0102-y","article-title":"Cold-induced epigenetic programming of the sperm enhances brown adipose tissue activity in the offspring","volume":"24","author":"Sun","year":"2018","journal-title":"Nat. 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