{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:21:50Z","timestamp":1771644110912,"version":"3.50.1"},"reference-count":132,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T00:00:00Z","timestamp":1634083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"United States Department of Agriculture, Agricultural Research Service","award":["5030-21220- 006-00D"],"award-info":[{"award-number":["5030-21220- 006-00D"]}]},{"DOI":"10.13039\/100012009","name":"United Soybean Board","doi-asserted-by":"publisher","award":["1920-172-0116-C"],"award-info":[{"award-number":["1920-172-0116-C"]}],"id":[{"id":"10.13039\/100012009","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>The soybean (Glycine max\u00a0L. merr) genotype Fiskeby III is highly resistant to a multitude of abiotic stresses, including iron deficiency, incurring only mild yield loss during stress conditions. Conversely, Mandarin (Ottawa) is highly susceptible to disease and suffers severe phenotypic damage and yield loss when exposed to abiotic stresses such as iron deficiency, a major challenge to soybean production in the northern Midwestern United States. Using RNA-seq, we characterize the transcriptional response to iron deficiency in both Fiskeby III and Mandarin (Ottawa) to better understand abiotic stress tolerance. Previous work by our group identified a quantitative trait locus (QTL) on chromosome 5 associated with Fiskeby III iron efficiency, indicating Fiskeby III utilizes iron deficiency stress mechanisms not previously characterized in soybean. We targeted 10 of the potential candidate genes in the Williams 82 genome sequence associated with the QTL using virus-induced gene silencing. Coupling virus-induced gene silencing with RNA-seq, we identified a single high priority candidate gene with a significant impact on iron deficiency response pathways. Characterization of the Fiskeby III responses to iron stress and the genes underlying the chromosome 5 QTL provides novel targets for improved abiotic stress tolerance in soybean.<\/jats:p>","DOI":"10.3390\/ijms222011032","type":"journal-article","created":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T21:36:15Z","timestamp":1634160975000},"page":"11032","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Mining Fiskeby III and Mandarin (Ottawa) Expression Profiles to Understand Iron Stress Tolerant Responses in Soybean"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2403-8946","authenticated-orcid":false,"given":"Jamie A.","family":"O\u2019Rourke","sequence":"first","affiliation":[{"name":"USDA-ARS, CICGRU, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J.","family":"Morrisey","sequence":"additional","affiliation":[{"name":"USDA-ARS, CICGRU, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryan","family":"Merry","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agronomy, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mary Jane","family":"Espina","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agronomy, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron J.","family":"Lorenz","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agronomy, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert M.","family":"Stupar","sequence":"additional","affiliation":[{"name":"Department of Genetics and Agronomy, University of Minnesota, St. Paul, MN 55108, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9842-7701","authenticated-orcid":false,"given":"Michelle A.","family":"Graham","sequence":"additional","affiliation":[{"name":"USDA-ARS, CICGRU, Ames, IA 50011, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1104\/pp.80.1.175","article-title":"Evidence for a specific uptake system for iron phytosiderophores in roots of grasses","volume":"80","author":"Romheld","year":"1986","journal-title":"Plant Physiol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1111\/j.1469-8137.2009.02908.x","article-title":"Dissecting iron deficiency-induced proton extrusion in Arabidopsis roots","volume":"183","author":"Santi","year":"2009","journal-title":"New Phytol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"694","DOI":"10.1038\/17800","article-title":"A ferric-chelate reductase for iron uptake from soils","volume":"397","author":"Robinson","year":"1999","journal-title":"Nature"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1223","DOI":"10.1105\/tpc.001388","article-title":"IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growth","volume":"14","author":"Vert","year":"2002","journal-title":"Plant Cell"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"538","DOI":"10.1016\/j.tplants.2017.03.008","article-title":"Mobilization of iron by plant-borne coumarins","volume":"22","author":"Tsai","year":"2017","journal-title":"Trends Plant Sci."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1146\/annurev-arplant-042811-105522","article-title":"Iron uptake, translocation, and regulation in higher plants","volume":"63","author":"Kobayashi","year":"2012","journal-title":"Annu. 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