{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T01:11:09Z","timestamp":1790644269371,"version":"4.1.0"},"reference-count":43,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T00:00:00Z","timestamp":1634169600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Investissement d'Avenir Infrastructures Nationales en Biologie et Sant\u00e9, ProFI project","award":["ANR-10-INBS-08"],"award-info":[{"award-number":["ANR-10-INBS-08"]}]},{"name":"French National Research Agency ANR, LIFE project","award":["ANR-15-IDEX-02"],"award-info":[{"award-number":["ANR-15-IDEX-02"]}]},{"name":"Chemistry Biology Health Graduate School of University Grenoble Alpes","award":["ANR-17-EURE-0003"],"award-info":[{"award-number":["ANR-17-EURE-0003"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Acute liver injury (ALI) is a severe disorder resulting from excessive hepatocyte cell death, and frequently caused by acetaminophen intoxication. Clinical management of ALI progression is hampered by the dearth of blood biomarkers available. In this study, a bioinformatics workflow was developed to screen omics databases and identify potential biomarkers for hepatocyte cell death. Then, discovery proteomics was harnessed to select from among these candidates those that were specifically detected in the blood of acetaminophen-induced ALI patients. Among these candidates, the isoenzyme alcohol dehydrogenase 1B (ADH1B) was massively leaked into the blood. To evaluate ADH1B, we developed a targeted proteomics assay and quantified ADH1B in serum samples collected at different times from 17 patients admitted for acetaminophen-induced ALI. Serum ADH1B concentrations increased markedly during the acute phase of the disease, and dropped to undetectable levels during recovery. In contrast to alanine aminotransferase activity, the rapid drop in circulating ADH1B concentrations was followed by an improvement in the international normalized ratio (INR) within 10\u201348 h, and was associated with favorable outcomes. In conclusion, the combination of omics data exploration and proteomics revealed ADH1B as a new blood biomarker candidate that could be useful for the monitoring of acetaminophen-induced ALI.<\/jats:p>","DOI":"10.3390\/ijms222011071","type":"journal-article","created":{"date-parts":[[2021,10,14]],"date-time":"2021-10-14T09:10:49Z","timestamp":1634202649000},"page":"11071","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Mass Spectrometry-Based Proteomics Reveal Alcohol Dehydrogenase 1B as a Blood Biomarker Candidate to Monitor Acetaminophen-Induced Liver Injury"],"prefix":"10.3390","volume":"22","author":[{"given":"Floriane","family":"Pailleux","sequence":"first","affiliation":[{"name":"Univ. Grenoble Alpes, INSERM, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, FR2048, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pauline","family":"Maes","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, INSERM, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, FR2048, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Jaquinod","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, INSERM, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, FR2048, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Justine","family":"Barthelon","sequence":"additional","affiliation":[{"name":"Univ. Grenoble Alpes, INSERM, CEA, UMR BioSant\u00e9 U1292, CNRS, CEA, FR2048, 38000 Grenoble, France"},{"name":"Clinique Universitaire d\u2019H\u00e9pato-gastroent\u00e9rologie, Centre Hospitalier Universitaire Grenoble, 38000 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0919-8303","authenticated-orcid":false,"given":"Marion","family":"Darnaud","sequence":"additional","affiliation":[{"name":"Hepatobiliary Centre, Paul-Brousse University Hospital, INSERM U1193, 94800 Villejuif, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7013-2533","authenticated-orcid":false,"given":"Claire","family":"Lacoste","sequence":"additional","affiliation":[{"name":"Hepatobiliary Centre, Paul-Brousse University Hospital, INSERM U1193, 94800 Villejuif, France"},{"name":"Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris-Sud, Universit\u00e9 Paris-Saclay, 94270 Le Kremlin-Bic\u00eatre, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1292-373X","authenticated-orcid":false,"given":"Yves","family":"Vandenbrouck","sequence":"additional","affiliation":[{"name":"Univ. 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Gastroenterol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1002\/lt.23370","article-title":"Comparison of the sequential organ failure assessment score with the King\u2019s College Hospital criteria and the model for end-stage liver disease score for the prognosis of acetaminophen-induced acute liver failure","volume":"18","author":"Cholongitas","year":"2012","journal-title":"Liver Transpl."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1016\/j.cgh.2015.10.007","article-title":"Ability of King\u2019s College Criteria and Model for End-Stage Liver Disease Scores to Predict Mortality of Patients with Acute Liver Failure: A Meta-analysis","volume":"14","author":"McPhail","year":"2016","journal-title":"Clin. Gastroenterol. Hepatol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.3748\/wjg.v22.i4.1523","article-title":"Liver transplantation in acute liver failure: A challenging scenario","volume":"22","author":"Mendizabal","year":"2016","journal-title":"World J. Gastroenterol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"593","DOI":"10.1016\/j.jhep.2010.06.002","article-title":"Prognosis indicator in acute liver failure: Is there a place for cell death markers?","volume":"53","author":"Samuel","year":"2010","journal-title":"J. Hepatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1053\/j.gastro.2012.07.011","article-title":"Pathogenesis of liver injury in acute liver failure","volume":"143","author":"Chung","year":"2012","journal-title":"Gastroenterology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1016\/j.clinthera.2015.07.012","article-title":"Changing the Management of Paracetamol Poisoning","volume":"37","author":"Bateman","year":"2015","journal-title":"Clin. Ther."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"439","DOI":"10.1016\/0016-5085(89)90081-4","article-title":"Early indicators of prognosis in fulminant hepatic failure","volume":"97","author":"Alexander","year":"1989","journal-title":"Gastroenterology"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1063","DOI":"10.1016\/j.jhep.2013.12.026","article-title":"Biomarkers of liver cell death","volume":"60","author":"Eguchi","year":"2014","journal-title":"J. Hepatol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1324","DOI":"10.1002\/lt.21236","article-title":"Predictive value of actin-free Gc-globulin in acute liver failure","volume":"13","author":"Schiodt","year":"2007","journal-title":"Liver Transpl."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1111\/bcp.12699","article-title":"Target biomarker profile for the clinical management of paracetamol overdose","volume":"80","author":"Vliegenthart","year":"2015","journal-title":"Br. J. Clin. Pharmacol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"e1800489","DOI":"10.1002\/pmic.201800489","article-title":"Bioinformatics Tools and Workflow to Select Blood Biomarkers for Early Cancer Diagnosis: An Application to Pancreatic Cancer","volume":"19","author":"Vandenbrouck","year":"2019","journal-title":"Proteomics"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"1260419","DOI":"10.1126\/science.1260419","article-title":"Proteomics. Tissue-based map of the human proteome","volume":"347","author":"Uhlen","year":"2015","journal-title":"Science"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2139","DOI":"10.1074\/mcp.M700163-MCP200","article-title":"Isotope-labeled protein standards: Toward absolute quantitative proteomics","volume":"6","author":"Brun","year":"2007","journal-title":"Mol. Cell. Proteomics"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/978-1-4939-9164-8_18","article-title":"Designing an in Silico Strategy to Select Tissue-Leakage Biomarkers Using the Galaxy Framework","volume":"1959","author":"Nguyen","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"862","DOI":"10.15252\/msb.20155865","article-title":"Transcriptomics resources of human tissues and organs","volume":"12","author":"Uhlen","year":"2016","journal-title":"Mol. Syst. Biol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"D76","DOI":"10.1093\/nar\/gkr1179","article-title":"neXtProt: A knowledge platform for human proteins","volume":"40","author":"Lane","year":"2012","journal-title":"Nucleic Acids Res."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4299","DOI":"10.1021\/acs.jproteome.7b00467","article-title":"The Human Plasma Proteome Draft of 2017: Building on the Human Plasma PeptideAtlas from Mass Spectrometry and Complementary Assays","volume":"16","author":"Schwenk","year":"2017","journal-title":"J. Proteome Res."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2631","DOI":"10.1021\/ac300006b","article-title":"Consecutive proteolytic digestion in an enzyme reactor increases depth of proteomic and phosphoproteomic analysis","volume":"84","author":"Wisniewski","year":"2012","journal-title":"Anal. Chem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1016\/j.talanta.2019.05.028","article-title":"Quantification of surfactant protein D (SPD) in human serum by liquid chromatography-mass spectrometry (LC-MS)","volume":"202","author":"Klont","year":"2019","journal-title":"Talanta"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"570","DOI":"10.1038\/sj.mp.4001497","article-title":"Genetic polymorphism in ethanol metabolism: Acetaldehyde contribution to alcohol abuse and alcoholism","volume":"9","author":"Quertemont","year":"2004","journal-title":"Mol. Psychiatry"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1002\/pmic.201100538","article-title":"Mass spectrometry-based absolute protein quantification: PSAQ (TM) strategy makes use of \u201cnoncanonical\u201d proteotypic peptides","volume":"12","author":"Jaquinod","year":"2012","journal-title":"Proteomics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1074\/mcp.E117.067801","article-title":"New Guidelines for Publication of Manuscripts Describing Development and Application of Targeted Mass Spectrometry Measurements of Peptides and Proteins","volume":"16","author":"Abbatiello","year":"2017","journal-title":"Mol. Cell. Proteomics"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1373\/clinchem.2014.224840","article-title":"From lost in translation to paradise found: Enabling protein biomarker method transfer by mass spectrometry","volume":"60","author":"Grant","year":"2014","journal-title":"Clin. Chem."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1781","DOI":"10.4155\/bio-2018-0268","article-title":"2018 White Paper on Recent Issues in Bioanalysis: \u2018A global bioanalytical community perspective on last decade of incurred samples reanalysis (ISR)\u2019 (Part 1\u2014Small molecule regulated bioanalysis, small molecule biomarkers, peptides & oligonucleotide bioanalysis)","volume":"10","author":"Welink","year":"2018","journal-title":"Bioanalysis"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2152","DOI":"10.1093\/clinchem\/35.10.2152","article-title":"Determination of the Lower Limit of Detection","volume":"35","author":"Anderson","year":"1989","journal-title":"Clin. Chem."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"D959","DOI":"10.1093\/nar\/gkt1251","article-title":"Plasma Proteome Database as a resource for proteomics research: 2014 update","volume":"42","author":"Nanjappa","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"22","DOI":"10.1016\/j.toxlet.2019.05.005","article-title":"Insights into mechanisms and severity of drug-induced liver injury via computational systems toxicology approach","volume":"312","author":"Peng","year":"2019","journal-title":"Toxicol. Lett."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Urman, J.M., Herranz, J.M., Uriarte, I., Rullan, M., Oyon, D., Gonzalez, B., Fernandez-Urien, I., Carrascosa, J., Bolado, F., and Zabalza, L. (2020). Pilot Multi-Omic Analysis of Human Bile from Benign and Malignant Biliary Strictures: A Machine-Learning Approach. Cancers, 12.","DOI":"10.3390\/cancers12061644"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s10565-018-9431-3","article-title":"Clinical trans-omics: An integration of clinical phenomes with molecular multiomics","volume":"34","author":"Wang","year":"2018","journal-title":"Cell Biol. Toxicol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Prassas, I., Chrystoja, C.C., Makawita, S., and Diamandis, E.P. (2012). Bioinformatic identification of proteins with tissue-specific expression for biomarker discovery. BMC Med., 10.","DOI":"10.1186\/1741-7015-10-39"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"3724","DOI":"10.1021\/acs.jproteome.6b00547","article-title":"Identification of Organ-Enriched Protein Biomarkers of Acute Liver Injury by Targeted Quantitative Proteomics of Blood in Acetaminophen- and Carbon-Tetrachloride-Treated Mouse Models and Acetaminophen Overdose Patients","volume":"15","author":"Qin","year":"2016","journal-title":"J. Proteome Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1016\/S2468-1253(17)30266-2","article-title":"Risk stratification after paracetamol overdose using mechanistic biomarkers: Results from two prospective cohort studies","volume":"3","author":"Dear","year":"2018","journal-title":"Lancet Gastroenterol. Hepatol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"571","DOI":"10.1002\/ueg2.12093","article-title":"Plasma procalcitonin may be an early predictor of liver injury in acetaminophen poisoning: A prospective cohort study","volume":"9","author":"Nuzzo","year":"2021","journal-title":"UEG J."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1007\/978-1-61779-148-2_7","article-title":"Production and Use of Stable Isotope-Labeled Proteins for Absolute Quantitative Proteomics","volume":"Volume 753","author":"Lebert","year":"2011","journal-title":"Gel-Free Proteomics"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1016\/j.talanta.2017.04.042","article-title":"Introducing plasma\/serum glycodepletion for the targeted proteomics analysis of cytolysis biomarkers","volume":"170","author":"Maes","year":"2017","journal-title":"Talanta"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"3929","DOI":"10.1021\/pr3003326","article-title":"Introducing AAA-MS, a Rapid and Sensitive Method for Amino Acid Analysis Using Isotope Dilution and High-Resolution Mass Spectrometry","volume":"11","author":"Louwagie","year":"2012","journal-title":"J. Proteome Res."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1007\/978-1-4939-9164-8_9","article-title":"Protein Biomarker Discovery in Non-depleted Serum by Spectral Library-Based Data-Independent Acquisition Mass Spectrometry","volume":"1959","author":"Kraut","year":"2019","journal-title":"Methods Mol. Biol."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"3148","DOI":"10.1093\/bioinformatics\/btaa118","article-title":"Proline: An efficient and user-friendly software suite for large-scale proteomics","volume":"36","author":"Bouyssie","year":"2020","journal-title":"Bioinformatics"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1093\/bioinformatics\/btw580","article-title":"DAPAR & ProStaR: Software to perform statistical analyses in quantitative discovery proteomics","volume":"33","author":"Wieczorek","year":"2017","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"966","DOI":"10.1093\/bioinformatics\/btq054","article-title":"Skyline: An open source document editor for creating and analyzing targeted proteomics experiments","volume":"26","author":"MacLean","year":"2010","journal-title":"Bioinformatics"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"D442","DOI":"10.1093\/nar\/gky1106","article-title":"The PRIDE database and related tools and resources in 2019: Improving support for quantification data","volume":"47","author":"Csordas","year":"2019","journal-title":"Nucleic Acids Res."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11071\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:14:28Z","timestamp":1760166868000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,14]]},"references-count":43,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ijms222011071"],"URL":"https:\/\/doi.org\/10.3390\/ijms222011071","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,14]]}}}