{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:23:59Z","timestamp":1787505839940,"version":"build-2736575974"},"reference-count":60,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T00:00:00Z","timestamp":1634256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Thyme species are a good source of thymol and carvacrol, which play a key role in controlling diseases. For the first time, the expression patterns of \u03b3-terpinene synthase (TPS2), CYP71D178, and CYP71D180 genes and the amount of phenolics compounds were evaluated in T. migricus and T. daenensis after different methyl jasmonate (MeJA) treatments. The highest thymol and carvacrol contents were observed in T. migricus (86.27%) and T. daenensis (17.87%) at MeJA 100 \u00b5M, which was consistent with the expression patterns of the three investigated genes. All species treated showed high total phenolic and flavonoid content compared to control plants for which the highest amounts were observed in T. vulgaris treated with 100 \u00b5M and 10 \u00b5M MeJA. Furthermore, in the 100 \u00b5M MeJA treatment, the relative expression of TPS2 and CYP71D178 in T. migricus increased 7.47 and 9.86-fold compared with the control, respectively. The highest level of CYP71D180 transcripts (5.15-fold) was also observed for T. daenensis treated. This finding highlights the notion that thymol was known as the dominant component of the essential oil rather than carvacrol in diffident thyme species. This implies that MeJA at different concentrations influenced metabolic pathways and induced expression changes, resulting in a rise in essential oil levels.<\/jats:p>","DOI":"10.3390\/ijms222011124","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T13:35:47Z","timestamp":1634304947000},"page":"11124","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Effect of Methyl Jasmonate on Thymol, Carvacrol, Phytochemical Accumulation, and Expression of Key Genes Involved in Thymol\/Carvacrol Biosynthetic Pathway in Some Iranian Thyme Species"],"prefix":"10.3390","volume":"22","author":[{"given":"Farzad","family":"Kianersi","sequence":"first","affiliation":[{"name":"Department of Agronomy and Plant Breeding, Faculty of Agriculture, Bu-Ali Sina University, Hamedan P.O. Box 6517838695, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5782-5327","authenticated-orcid":false,"given":"Alireza","family":"Pour-Aboughadareh","sequence":"additional","affiliation":[{"name":"Seed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3183964653, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad","family":"Majdi","sequence":"additional","affiliation":[{"name":"Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj P.O. Box 1517566177, Iran"},{"name":"Research Center for Medicinal Plant Breeding and Development, University of Kurdistan, Sanandaj P.O. Box 1517566177, Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0107-1068","authenticated-orcid":false,"given":"Peter","family":"Poczai","sequence":"additional","affiliation":[{"name":"Botany Unit, Finnish Museum of Natural History, University of Helsinki, P.O. Box 7, 00014 Helsinki, Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1016\/j.indcrop.2018.12.046","article-title":"Salicylic acid induced changes in physiological traits and essential oil constituents in different ecotypes of Thymus kotschyanus and Thymus vulgaris under well-watered and water stress conditions","volume":"129","author":"Mohammadi","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_2","first-page":"291","article-title":"Antibacterial activity of Thymus pubescens methanolic extract","volume":"7","author":"Mehrgan","year":"2008","journal-title":"Iran. J. Pharm. Sci."},{"key":"ref_3","first-page":"657026","article-title":"Effects of thymol and carvacrol, constituents of Thymus vulgaris L. essential oil, on the inflammatory response","volume":"2012","author":"Kummer","year":"2012","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_4","first-page":"345","article-title":"Composition and antioxidant activity of the essential oils of Thymus trautvetteri Klokov and Des.-Shost., Thymus migricus Klokov and Des.-Shost. and Thymus caespititius Brot. from Iran","volume":"9","author":"Farjam","year":"2013","journal-title":"Nat. Prod. Indian J."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1080\/0972060X.2013.764190","article-title":"Essential oil composition, total phenolic content, antioxidant activity and antifungal properties of Iranian Thymus daenensis subsp. daenensis Celak. as in influenced by ontogenetical variation","volume":"16","author":"Alizadeh","year":"2013","journal-title":"J. Essent. Oil-Bear. Plants"},{"key":"ref_6","first-page":"205","article-title":"Chemical composition and antimicrobial activity of essential oil of thyme (Thymus vulgaris) from Eastern Morocco","volume":"11","author":"Imelouane","year":"2009","journal-title":"Int. J. Agric. Biol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1272","DOI":"10.1080\/0972060X.2018.1533435","article-title":"Variations in chemical composition and bioactive compounds of Thymus kotschyanus Boiss. and Hohen populations originated from different collection sites","volume":"21","author":"Tohidi","year":"2018","journal-title":"J. Essent. Oil-Bear. Plants"},{"key":"ref_8","first-page":"190","article-title":"Preliminary studies on antibacterial and antiviral activities of five medicinal plants","volume":"4","year":"2013","journal-title":"J. Plant Pathol. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Moreira-Rodr\u00edguez, M., Nair, V., Benavides, J., Cisneros-Zevallos, L., and Jacobo-Vel\u00e1zquez, D.A. (2017). UVA, UVB light, and methyl jasmonate, alone or combined, redirect the biosynthesis of glucosinolates, phenolics, carotenoids, and chlorophylls in broccoli sprouts. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18112330"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Santana-G\u00e1lvez, J., Cisneros-Zevallos, L., and Jacobo-Vel\u00e1zquez, D.A. (2017). Chlorogenic acid: Recent advances on its dual role as a food additive and a nutraceutical against metabolic syndrome. Molecules, 22.","DOI":"10.3390\/molecules22030358"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"728065","DOI":"10.1155\/2012\/728065","article-title":"Essential oils composition and antioxidant properties of three thymus species","volume":"2012","author":"Amiri","year":"2012","journal-title":"Evid.-Based Complement. Altern. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1016\/j.foodchem.2004.04.020","article-title":"Analysis of the essential oils of two Thymus species from Iran","volume":"90","author":"Nickavar","year":"2015","journal-title":"Food Chem."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"459","DOI":"10.1080\/10412905.2009.9700218","article-title":"Correlation of antimicrobial activities of various essential oils and their main aromatic volatile constituents","volume":"21","author":"Hoferl","year":"2009","journal-title":"J. Essent. Oil Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"258","DOI":"10.1016\/j.indcrop.2013.02.002","article-title":"Optimization of the antioxidant capacity of thyme (Thymus vulgaris L.) extracts: Management of the drying process","volume":"46","author":"Rodriguez","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_15","unstructured":"Crocoll, C. (2011). Biosynthesis of the Phenolic Monoterpenes, Thymol and Carvacrol, by Terpene Synthases and Cytochrome P450s in Oregano and Thyme. [Ph.D. Thesis, Biologisch-Pharmazeutischen Fakulte at der Friedrich-Schiller-Universite at Jena]."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1073\/pnas.0407360102","article-title":"The nonmevalonate pathway supports both monoterpene and sesquiterpene formation in snapdragon flowers","volume":"102","author":"Dudareva","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"9879","DOI":"10.1073\/pnas.95.17.9879","article-title":"A 1-deoxy-D-xylulose 5-phosphate reductoisomerase catalyzing the formation of 2-C-methyl-D-erythritol 4-phosphate in an alternative nonmevalonate pathway for terpenoid biosynthesis","volume":"95","author":"Takahashi","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1146\/annurev.arplant.50.1.47","article-title":"The 1-deoxy-D-xylulose-5-phosphate pathway of isoprenoid biosynthesis in plants","volume":"50","author":"Lichtenthaler","year":"1999","journal-title":"Annu. Rev. Plant Physiol. Plant Mol. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"13062","DOI":"10.1073\/pnas.96.23.13062","article-title":"Geranyl diphosphate synthase: Cloning, expression, and characterization of this prenyltransferase as a heterodimer","volume":"9","author":"Burke","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"152","DOI":"10.1016\/j.plaphy.2017.03.016","article-title":"Tissue-specific gene-expression patterns of genes associated with thymol\/carvacrol biosynthesis in thyme (Thymus vulgaris L.) and their differential changes upon treatment with abiotic elicitors","volume":"115","author":"Majdi","year":"2017","journal-title":"Plant Physiol. Biochem."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"219","DOI":"10.1111\/ppl.12168","article-title":"Dynamic changes in plant secondary metabolites during UV acclimation in Arabidopsis thaliana","volume":"152","author":"Hectors","year":"2014","journal-title":"Physiol. Plant."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1007\/s11104-015-2677-0","article-title":"Differential deployment of parthenolide and phenylpropanoids in feverfew plants subjected to divalent heavy metals and transcinnamic acid","volume":"399","author":"Hojati","year":"2016","journal-title":"Plant Soil."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1909","DOI":"10.1007\/s00299-015-1837-2","article-title":"Parthenolide accumulation and expression of genes related to parthenolide biosynthesis affected by exogenous application of methyl jasmonate and salicylic acid in Tanacetum parthenium","volume":"34","author":"Majdi","year":"2015","journal-title":"Plant Cell Rep."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1016\/j.indcrop.2015.11.005","article-title":"Spatial modulation and abiotic elicitors responses of the biosynthesis related genes of mono\/triterpenes in blak cumin (Nigella sativa)","volume":"79","author":"Elyasi","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"561","DOI":"10.1007\/s11240-018-01537-8","article-title":"Identification and expression profiling of rosmarinic acid biosynthetic genes from Satureja khuzistanica under carbon nanotubes and methyl jasmonate elicitation","volume":"136","author":"Fatemi","year":"2019","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"286","DOI":"10.1080\/13880209.2020.1743324","article-title":"Phytochemical, antioxidant, enzyme activity and antifungal properties of Satureja khuzistanica in vitro and in vivo explants stimulated by some chemical elicitors","volume":"58","author":"Fatemi","year":"2020","journal-title":"Pharm. Biol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1038\/s41598-020-65815-2","article-title":"Identification and tissue-specific expression of rutin biosynthetic pathway genes in Capparis spinosa elicited with salicylic acid and methyl jasmonate","volume":"10","author":"Kianersi","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"619","DOI":"10.1007\/s11240-020-01823-4","article-title":"Biosynthesis of rutin changes in Capparis spinosa due to altered expression of its pathway genes under elicitors\u2019 supplementation","volume":"141","author":"Kianersi","year":"2020","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1016\/j.tplants.2012.03.001","article-title":"Transcriptional machineries in jasmonate-elicited plant secondary metabolism","volume":"17","author":"Gholami","year":"2012","journal-title":"Trends Plant Sci."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"269","DOI":"10.1016\/j.indcrop.2015.06.052","article-title":"Changes in the essential oil content and selected traits of sweet basil (Ocimum basilicum L.) as induced by foliar sprays of citric acid and salicylic acid","volume":"76","author":"Mirzajani","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_31","first-page":"1811","article-title":"Plant defences against ants provide a pathway to social parasitism in butterflies","volume":"282","author":"Patricelli","year":"2015","journal-title":"Proc. R. Soc."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1016\/j.indcrop.2016.02.046","article-title":"Stimulated production of triterpenoids of Inonotus obliquus using methyl jasmonate and fatty acids","volume":"85","author":"Xu","year":"2016","journal-title":"Ind. Crops Prod."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"125521","DOI":"10.1016\/j.foodchem.2019.125521","article-title":"Thymol, carvacrol, and antioxidant accumulation in Thymus species in response to different light spectra emitted by light-emitting diodes","volume":"307","author":"Tohidi","year":"2020","journal-title":"Food Chem."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"233","DOI":"10.4103\/0250-474X.91570","article-title":"Seasonal variation in essential oil content and composition of Thyme, Thymus serpyllum L. cultivated in Uttarakhand hills","volume":"73","author":"Verma","year":"2011","journal-title":"Indian J. Pharm. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1016\/j.foodchem.2016.06.070","article-title":"Inhibitory effect of different fennel (Foeniculum vulgare) samples and their phenolic compounds on formation of advanced glycation products and comparison of antimicrobial and antioxidant activities","volume":"213","author":"Salami","year":"2016","journal-title":"Food Chem."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1382","DOI":"10.1080\/0972060X.2014.981600","article-title":"Comparison of essential oil composition, flavonoid content and antioxidant activity in eight Achillea species","volume":"18","author":"Gharibi","year":"2015","journal-title":"J. Essent. Oil-Bear. Plants"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"437","DOI":"10.1016\/j.indcrop.2012.10.012","article-title":"Antioxidant and Antimicrobial Activities of Essential Oil and Extracts of Fennel (Foeniculum vulgare L.) and Chamomile (Matricaria chamomilla L.)","volume":"44","author":"Roby","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.jff.2012.04.007","article-title":"Research on the phenolic compounds and antioxidant activities of Tunisian Thymus capitatus","volume":"4","author":"Bettaieb","year":"2012","journal-title":"J. Funct. Foods"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"153","DOI":"10.1016\/j.foodchem.2016.09.203","article-title":"Essential oil composition, total phenolic, flavonoid contents, and antioxidant activity of Thymus species collected from different regions of Iran","volume":"220","author":"Tohidi","year":"2017","journal-title":"Food Chem."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1016\/S2221-1691(13)60142-2","article-title":"Study of total phenol, flavonoids contents and phytochemical screening of various leaves crude extracts of locally grown Thymus vulgaris","volume":"3","author":"Hossain","year":"2013","journal-title":"Asian Pac. J. Trop Biomed."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1007\/s10528-009-9281-z","article-title":"Genetic variability and geographic differentiation in Thymus daenensis subsp. daenensis, an endangered medicinal plant, as revealed by inter simple sequence repeat (ISSR) markers","volume":"47","author":"Rahimmalek","year":"2009","journal-title":"Biochem. Genet."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Manivannan, A., Soundararajan, P., Park, Y.G., and Jeong, B.R. (2016). Chemical elicitor-induced modulation of antioxidant metabolism and enhancement of secondary metabolite accumulation in cell suspension cultures of Scrophularia kakudensis Franch. Int. J. Mol. Sci., 17.","DOI":"10.3390\/ijms17030399"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1186\/s12906-015-0914-y","article-title":"Antioxidant, antibacterial activity, and phytochemical characterization of Melaleuca cajuputi extract","volume":"15","author":"Nor","year":"2015","journal-title":"BMC Complement. Altern. Med."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"1524","DOI":"10.1016\/j.foodchem.2009.01.051","article-title":"GC\/MS analysis and in vitro antioxidant activity of essential oil and methanol extracts of Thymus caramanicus Jalas and its main constituent carvacrol","volume":"115","author":"Ebrahimabadi","year":"2009","journal-title":"Food Chem."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.foodcont.2003.10.005","article-title":"The in vitro antimicrobial and antioxidant activities of the essential oils and methanol extracts of endemic Thymus spathulifolius","volume":"15","author":"Sokmen","year":"2004","journal-title":"Food Control"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1016\/j.foodchem.2006.09.017","article-title":"Antioxidant and antiacetylcholinesterase activities of five plants used as Portuguese food spices","volume":"103","author":"Mata","year":"2007","journal-title":"Food Chem."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1016\/j.indcrop.2012.06.012","article-title":"Evaluation of six drying treatments with respect to essential oil yield, composition and color characteristics of Thymys daenensis subsp. daenensis. Celak leaves","volume":"42","author":"Rahimmalek","year":"2013","journal-title":"Ind. Crops Prod."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1016\/j.indcrop.2018.11.076","article-title":"Impact of mulching on growth essential oil composition and its biological activity in Monarda didyma L","volume":"129","author":"Szumny","year":"2019","journal-title":"Ind. Crops Prod."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"3741","DOI":"10.1021\/jf980087w","article-title":"Carnacini, A. Essential oil chemical composition of wild populations of italian oregano spice (Origanum vulgare ssp. hirtum (Link) Ietswaart): A preliminary evaluation of their use in chemotaxonomy by cluster analysis. 1. Inflorescences","volume":"46","author":"Russo","year":"1998","journal-title":"J. Agric. Food. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1007\/s11103-010-9636-1","article-title":"Terpene synthases of oregano (Origanum vulgare L.) and their roles in the pathway and regulation of terpene biosynthesis","volume":"73","author":"Crocoll","year":"2010","journal-title":"Plant. Mol. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1586","DOI":"10.1104\/pp.103.021196","article-title":"Induction of volatile terpene biosynthesis and diurnal emission by methyl jasmonate in foliage of Norway spruce","volume":"132","author":"Martin","year":"2003","journal-title":"Plant Physiol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"2652","DOI":"10.1021\/jf040421q","article-title":"Induction of de novo volatile terpene biosynthesis via cytosolic and plastidial pathways by methyl jasmonate in foliage of Vitis vinifera L.","volume":"53","author":"Hampel","year":"2005","journal-title":"J. Agric. Food Chem."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"699","DOI":"10.3390\/metabo4030699","article-title":"Recent advances in the application of metabolomics to studies of biogenic volatile organic compounds (BVOC) produced by plant","volume":"4","author":"Iijima","year":"2014","journal-title":"Metabolites"},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Jiang, A.L., Liu, Y.N., Liu, R., Ren, A., Ma, H.Y., Shu, L.B., Shi, L., Zhu, J., and Zhao, M.W. (2019). Integrated proteomics and metabolomics analysis provides insights into ganoderic acid biosynthesis in response to methyl jasmonate in Ganoderma lucidum. Int. J. Mol. Sci., 20.","DOI":"10.3390\/ijms20246116"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"6389","DOI":"10.1126\/science.aau6389","article-title":"A specialized metabolic network selectively modulates Arabidopsis root microbiota","volume":"364","author":"Huang","year":"2019","journal-title":"Science"},{"key":"ref_56","unstructured":"Rechinger, K.H. (1963). Flora Iranica, Verlagsanstalt Wien Austria Akademische Druke-U."},{"key":"ref_57","first-page":"112","article-title":"Sequencing and variation of terpene synthase gene (TPS2) as the major gene in biosynthesis of thymol in different Thymus species","volume":"1","author":"Tohidi","year":"2020","journal-title":"Phytochem"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394ct method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.indcrop.2015.09.048","article-title":"Variation of essential oil content and antioxidant activity of Lonicera species in different sites of China","volume":"77","author":"Zhang","year":"2015","journal-title":"Ind. Crops Prod."},{"key":"ref_60","first-page":"1902","article-title":"Identification of essential oil components by gas chromatography\/quadrupole mass spectrometry","volume":"16","author":"Adams","year":"2007","journal-title":"J. Am. Soc. Mass Spectrom."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11124\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:15:24Z","timestamp":1760166924000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/20\/11124"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,15]]},"references-count":60,"journal-issue":{"issue":"20","published-online":{"date-parts":[[2021,10]]}},"alternative-id":["ijms222011124"],"URL":"https:\/\/doi.org\/10.3390\/ijms222011124","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,15]]}}}