{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T23:12:16Z","timestamp":1788390736233,"version":"build-2803163510"},"reference-count":65,"publisher":"MDPI AG","issue":"20","license":[{"start":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T00:00:00Z","timestamp":1634256000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Natural Science Foundation of China, the State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, China , the National science and technology major special project of transgenes, the Science and Technology of Tianjin, China .","award":["No. 31872115, No. 31470669, NFUZD2015, 2018ZX08020003-001-003, No. 18ZXZYNC00160"],"award-info":[{"award-number":["No. 31872115, No. 31470669, NFUZD2015, 2018ZX08020003-001-003, No. 18ZXZYNC00160"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Plant thaumatin-like proteins (TLPs) play pleiotropic roles in defending against biotic and abiotic stresses. However, the functions of TLPs in broccoli, which is one of the major vegetables among the B. oleracea varieties, remain largely unknown. In the present study, bolTLP1 was identified in broccoli, and displayed remarkably inducible expression patterns by abiotic stress. The ectopic overexpression of bolTLP1 conferred increased tolerance to high salt and drought conditions in Arabidopsis. Similarly, bolTLP1-overexpressing broccoli transgenic lines significantly improved tolerance to salt and drought stresses. These results demonstrated that bolTLP1 positively regulates drought and salt tolerance. Transcriptome data displayed that bolTLP1 may function by regulating phytohormone (ABA, ethylene and auxin)-mediated signaling pathways, hydrolase and oxidoreductase activity, sulfur compound synthesis, and the differential expression of histone variants. Further studies confirmed that RESPONSE TO DESICCATION 2 (RD2), RESPONSIVE TO DEHYDRATION 22 (RD22), VASCULAR PLANT ONE-ZINC FINGER 2 (VOZ2), SM-LIKE 1B (LSM1B) and MALATE DEHYDROGENASE (MDH) physically interacted with bolTLP1, which implied that bolTLP1 could directly interact with these proteins to confer abiotic stress tolerance in broccoli. These findings provide new insights into the function and regulation of bolTLP1, and suggest potential applications for bolTLP1 in breeding broccoli and other crops with increased tolerance to salt and drought stresses.<\/jats:p>","DOI":"10.3390\/ijms222011132","type":"journal-article","created":{"date-parts":[[2021,10,15]],"date-time":"2021-10-15T13:35:47Z","timestamp":1634304947000},"page":"11132","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":46,"title":["BolTLP1, a Thaumatin-like Protein Gene, Confers Tolerance to Salt and Drought Stresses in Broccoli (Brassica oleracea L. var. Italica)"],"prefix":"10.3390","volume":"22","author":[{"given":"Lixia","family":"He","sequence":"first","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinxia","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muzi","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengbin","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Li","sequence":"additional","affiliation":[{"name":"College of Horticulture and Landscape, Tianjin Agricultural University, Tianjin 300384, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunguo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Genetics and Cell Biology, College of Life Sciences, Nankai University, Tianjin 300071, China"},{"name":"State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, Harbin 150040, China"},{"name":"State Key Laboratory of Vegetable Germplasm Innovation, Tianjin Academy of Agricultural Sciences, Tianjin 300381, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s00299-010-0826-8","article-title":"The superfamily of thaumatin-like proteins: Its origin, evolution, and expression towards biological function","volume":"29","author":"Liu","year":"2010","journal-title":"Plant Cell Rep."},{"key":"ref_2","first-page":"1531","article-title":"A tobacco mosaic virus-induced tobacco protein is homologous to the sweet-testing protein thaumatin","volume":"32","author":"Cornelissen","year":"1986","journal-title":"Nature"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"883","DOI":"10.1007\/s00425-008-0790-5","article-title":"Crystal structure analysis of NP24-I, A thaumatin-like protein","volume":"228","author":"Ghosh","year":"2008","journal-title":"Planta"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Petre, B., Major, I., Rouhier, N., and Duplessis, S. 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