{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T22:47:32Z","timestamp":1783032452604,"version":"3.54.6"},"reference-count":67,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T00:00:00Z","timestamp":1634860800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>A series of eight copper (II) complexes with 3-(4-chloro-3-nitrophenyl)thiourea were designed and synthesized. The cytotoxic activity of all compounds was assessed in three human cancer cell lines (SW480, SW620, PC3) and human normal keratinocytes (HaCaT). The complexes 1, 3, 5, 7 and 8 were cytotoxic to the studied tumor cells in the low micromolar range, without affecting the normal cells. The complexes 1, 3, 7 and 8 induced lactate dehydrogenase (LDH) release in all cancer cell lines, but not in the HaCaT cells. They provoked early apoptosis in pathological cells, especially in SW480 and PC3 cells. The ability of compounds 1, 3, 7 and 8 to diminish interleukin-6 (IL-6) concentration in a cell was established. For the first time, the influence of the most promising Cu (II) complexes on intensities of detoxifying and reactive oxygen species (ROS) scavenging the enzymes of tumor cells was studied. The cytotoxic effect of all copper (II) conjugates against standard and hospital bacterial strains was also proved.<\/jats:p>","DOI":"10.3390\/ijms222111415","type":"journal-article","created":{"date-parts":[[2021,10,22]],"date-time":"2021-10-22T09:18:09Z","timestamp":1634894289000},"page":"11415","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["The Cytotoxic Effect of Copper (II) Complexes with Halogenated 1,3-Disubstituted Arylthioureas on Cancer and Bacterial Cells"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6928-4561","authenticated-orcid":false,"given":"Alicja","family":"Chrzanowska","sequence":"first","affiliation":[{"name":"Chair and Department of Biochemistry, Medical University of Warsaw, 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Drzewiecka-Antonik","sequence":"additional","affiliation":[{"name":"Laboratory of X-ray and Electron Microscopy Research, Institute of Physics, Polish Academy of Sciences, 02-668 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Dobrzy\u0144ska","sequence":"additional","affiliation":[{"name":"Students\u2019 Scientific Society at Department of Pharmaceutical Microbiology, Faculty of Pharmacy, Warsaw Medical University, 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Stefa\u0144ska","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Microbiology, Centre for Preclinical Research, Medical University of Warsaw, 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7808-1280","authenticated-orcid":false,"given":"Piotr","family":"Pietrzyk","sequence":"additional","affiliation":[{"name":"Inorganic Chemistry Department, Faculty of Chemistry, Jagiellonian University, 30-387 Krak\u00f3w, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Struga","sequence":"additional","affiliation":[{"name":"Chair and Department of Biochemistry, Medical University of Warsaw, 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3269-9672","authenticated-orcid":false,"given":"Anna","family":"Bielenica","sequence":"additional","affiliation":[{"name":"Chair and Department of Biochemistry, Medical University of Warsaw, 02-097 Warszawa, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1021\/cr400327t","article-title":"Copper active sites in biology","volume":"114","author":"Solomon","year":"2014","journal-title":"Chem. 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