{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T05:52:09Z","timestamp":1785822729424,"version":"3.56.0"},"reference-count":175,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T00:00:00Z","timestamp":1635379200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["U19AI106683"],"award-info":[{"award-number":["U19AI106683"]}]},{"name":"NIH","award":["P01AI145818"],"award-info":[{"award-number":["P01AI145818"]}]},{"name":"NIH","award":["5R01AI137174"],"award-info":[{"award-number":["5R01AI137174"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Epithelial barrier disruption and failure of epithelial repair by aberrant epithelial-mesenchymal transition (EMT)-induced basal cells observed in nasal mucosa of chronic rhinosinusitis (CRS) are speculated to play important roles in disease pathophysiology. Microparticles (MPs) are a type of extracellular vesicle (EV) released by budding or shedding from the plasma membrane of activated or apoptotic cells. MPs are detected in nasal lavage fluids (NLFs) and are now receiving attention as potential biomarkers to evaluate the degree of activation of immune cells and injury of structural cells in nasal mucosa of subjects with sinus disease. There are three types of epithelial-cell-derived MPs, which are defined by the expression of different epithelial specific markers on their surface: EpCAM, E-cadherin, and integrin \u03b26 (ITGB6). When these markers are on MPs that are also carrying canonical EMT\/mesenchymal markers (Snail (SNAI1); Slug (SNAI2); alpha-smooth muscle actin (\u03b1SMA, ACTA2)) or pro- and anti-coagulant molecules (tissue factor (TF); tissue plasminogen activator (tPA); plasminogen activator inhibitor-1 (PAI-1)), they provide insight as to the roles of epithelial activation for EMT or regulation of coagulation in the underlying disease. In this review, we discuss the potential of epithelial MPs as research tools to evaluate status of nasal mucosae of CRS patients in the lab, as well as biomarkers for management and treatment of CRS in the clinic.<\/jats:p>","DOI":"10.3390\/ijms222111709","type":"journal-article","created":{"date-parts":[[2021,10,28]],"date-time":"2021-10-28T23:51:36Z","timestamp":1635465096000},"page":"11709","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Epithelial-Cell-Derived Extracellular Vesicles in Pathophysiology of Epithelial Injury and Repair in Chronic Rhinosinusitis: Connecting Immunology in Research Lab to Biomarkers in Clinics"],"prefix":"10.3390","volume":"22","author":[{"given":"Toru","family":"Takahashi","sequence":"first","affiliation":[{"name":"Division of Allergy-Immunology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert P","family":"Schleimer","sequence":"additional","affiliation":[{"name":"Division of Allergy-Immunology, Department of Medicine, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA"},{"name":"Department of Otolaryngology, Northwestern University Feinberg School of Medicine, Chicago, IL 60611, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1517","DOI":"10.1016\/j.jaci.2015.08.037","article-title":"Direct costs of adult chronic rhinosinusitis by using 4 methods of estimation: Results of the US Medical Expenditure Panel Survey","volume":"136","author":"Caulley","year":"2015","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1177\/000348941112000701","article-title":"Incremental Health Care Utilization and Expenditures for Chronic Rhinosinusitis in the United States","volume":"120","author":"Bhattacharyya","year":"2011","journal-title":"Ann. Otol. Rhinol. Laryngol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"333","DOI":"10.1016\/j.anai.2020.01.018","article-title":"Epithelial cell function and remodeling in nasal polyposis","volume":"124","author":"Bankova","year":"2020","journal-title":"Ann. Allergy Asthma Immunol."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1016\/j.jaci.2011.07.049","article-title":"Pathogenesis of chronic rhinosinusitis: Inflammation","volume":"128","author":"Zhang","year":"2011","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.jaci.2009.04.045","article-title":"Alterations in epithelial barrier function and host defense responses in chronic rhinosinusitis","volume":"124","author":"Tieu","year":"2009","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"331","DOI":"10.1146\/annurev-pathol-052016-100401","article-title":"Immunopathogenesis of Chronic Rhinosinusitis and Nasal Polyposis","volume":"12","author":"Schleimer","year":"2017","journal-title":"Annu. Rev. Pathol. Mech. Dis."},{"key":"ref_7","first-page":"649","article-title":"Allergic inflammatory memory in human respiratory epithelial progenitor cells","volume":"560","author":"Dwyer","year":"2018","journal-title":"Nat. Cell Biol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"682","DOI":"10.1164\/rccm.201412-2278OC","article-title":"Cytokines in Chronic Rhinosinusitis. Role in Eosinophilia and Aspirin-exacerbated Respiratory Disease","volume":"192","author":"Stevens","year":"2015","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"1449","DOI":"10.1016\/j.jaci.2015.12.1324","article-title":"Inflammatory endotypes of chronic rhinosinusitis based on cluster analysis of biomarkers","volume":"137","author":"Tomassen","year":"2016","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"944","DOI":"10.1164\/rccm.201109-1706OC","article-title":"Hypoxia-inducible Factor 1 Mediates Nasal Polypogenesis by Inducing Epithelial-to-Mesenchymal Transition","volume":"185","author":"Shin","year":"2012","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1540","DOI":"10.1111\/all.12503","article-title":"Features of mesenchymal transition in the airway epithelium from chronic rhinosinusitis","volume":"69","author":"Hupin","year":"2014","journal-title":"Allergy"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1895","DOI":"10.1016\/j.jaci.2018.02.002","article-title":"Identification of clinically relevant chronic rhinosinusitis endotypes using cluster analysis of mucus cytokines","volume":"141","author":"Turner","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"720","DOI":"10.1016\/j.jaci.2017.01.022","article-title":"Microparticles in nasal lavage fluids in chronic rhinosinusitis: Potential biomarkers for diagnosis of aspirin-exacerbated respiratory disease","volume":"140","author":"Takahashi","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1038\/nri2567","article-title":"Membrane vesicles as conveyors of immune responses","volume":"9","author":"Ostrowski","year":"2009","journal-title":"Nat. Rev. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"27066","DOI":"10.3402\/jev.v4.27066","article-title":"Biological properties of extracellular vesicles and their physiological functions","volume":"4","author":"Siljander","year":"2015","journal-title":"J. Extracell. Vesicles"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1510","DOI":"10.1164\/rccm.201612-2457PP","article-title":"Extracellular Vesicle Biology in the Pathogenesis of Lung Disease","volume":"196","author":"Acunzo","year":"2017","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_17","first-page":"24","article-title":"Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Angiogenesis: Potencial Clinical Application","volume":"7","author":"Zuniga","year":"2016","journal-title":"Front. Physiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"19743","DOI":"10.1038\/srep19743","article-title":"Characterization of Induced Pluripotent Stem Cell Microvesicle Genesis, Morphology and Pluripotent Content","volume":"6","author":"Zhou","year":"2016","journal-title":"Sci. Rep."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1275","DOI":"10.1164\/rccm.201701-0170OC","article-title":"Mesenchymal Stromal Cells Modulate Macrophages in Clinically Relevant Lung Injury Models by Extracellular Vesicle Mitochondrial Transfer","volume":"196","author":"Morrison","year":"2017","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3781","DOI":"10.1007\/s00018-018-2771-6","article-title":"Dissecting the biochemical architecture and morphological release pathways of the human platelet extracellular vesiculome","volume":"75","author":"Tegegn","year":"2018","journal-title":"Cell. Mol. Life Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1535750","DOI":"10.1080\/20013078.2018.1535750","article-title":"Minimal information for studies of extracellular vesicles 2018 (MISEV2018): A position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines","volume":"7","author":"Witwer","year":"2018","journal-title":"J. Extracell. Vesicles"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"108748","DOI":"10.1016\/j.biopha.2019.108748","article-title":"Emerging role of extracellular vesicles in lung injury and inflammation","volume":"113","author":"Lanyu","year":"2019","journal-title":"Biomed. Pharmacother."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"S71","DOI":"10.1016\/j.thromres.2012.08.281","article-title":"Microparticles in vascular disorders: How tissue factor-exposing vesicles contribute to pathology and physiology","volume":"130","author":"Kleinjan","year":"2012","journal-title":"Thromb. Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"527","DOI":"10.2147\/COPD.S99547","article-title":"Microparticles in sputum of COPD patients: A potential biomarker of the disease?","volume":"11","author":"Porro","year":"2016","journal-title":"Int. J. Chronic Obstr. Pulm. Dis."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1172\/JCI81129","article-title":"Extracellular vesicle isolation and characterization: Toward clinical application","volume":"126","author":"Xu","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3109\/01902148.2013.784932","article-title":"Differences in the released endothelial microparticle subtypes between human pulmonary microvascular endothelial cells and aortic endothelial cells in vitro","volume":"39","author":"Takahashi","year":"2013","journal-title":"Exp. Lung Res."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S0049-3848(03)00064-1","article-title":"Endothelial cells release phenotypically and quantitatively distinct microparticles in activation and apoptosis","volume":"109","author":"Jimenez","year":"2003","journal-title":"Thromb. Res."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"303","DOI":"10.2147\/COPD.S38931","article-title":"The role of microparticles in chronic obstructive pulmonary disease","volume":"9","author":"Kubo","year":"2014","journal-title":"Int. J. Chronic Obstr. Pulm. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2972","DOI":"10.1093\/eurheartj\/ehu153","article-title":"Association of circulating endothelial microparticles with cardiometabolic risk factors in the Framingham Heart Study","volume":"35","author":"Amabile","year":"2014","journal-title":"Eur. Hear. J."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"3381","DOI":"10.1681\/ASN.2005050535","article-title":"Circulating Endothelial Microparticles Are Associated with Vascular Dysfunction in Patients with End-Stage Renal Failure","volume":"16","author":"Amabile","year":"2005","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1067","DOI":"10.1136\/thoraxjnl-2011-201395","article-title":"Increased circulating endothelial microparticles in COPD patients: A potential biomarker for COPD exacerbation susceptibility","volume":"67","author":"Takahashi","year":"2012","journal-title":"Thorax"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"1148","DOI":"10.1164\/rccm.200712-1835OC","article-title":"Circulating Microparticles from Patients with Septic Shock Exert Protective Role in Vascular Function","volume":"178","author":"Mostefai","year":"2008","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_33","first-page":"e937","article-title":"Endothelial and leukocyte-derived microvesicles and cardiovascular risk after stroke\u2014PROSCIS-B","volume":"96","author":"Huo","year":"2020","journal-title":"Neurol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"266","DOI":"10.1183\/16000617.0009-2016","article-title":"Cell-derived microparticles and the lung","volume":"25","author":"Nieri","year":"2016","journal-title":"Eur. Respir. Rev."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Novelli, F., Neri, T., Tavanti, L., Armani, C., Noce, C., Falaschi, F., Bartoli, M.L., Martino, F., Palla, A., and Celi, A. (2014). Procoagulant, Tissue Factor-Bearing Microparticles in Bronchoalveolar Lavage of Interstitial Lung Disease Patients: An Observational Study. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0095013"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/cc9978","article-title":"High levels of circulating leukocyte microparticles are associated with better outcome in acute respiratory distress syndrome","volume":"15","author":"Guervilly","year":"2011","journal-title":"Crit. Care"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3261","DOI":"10.1111\/all.14419","article-title":"Integrin \u03b26 microparticles in nasal lavage fluids; potential new biomarkers for basal cell activation in chronic rhinosinusitis","volume":"75","author":"Takahashi","year":"2020","journal-title":"Allergy"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"PA3617","DOI":"10.1183\/13993003.congress-2015.PA3617","article-title":"Increased circulating alveolar epithelial microparticles in COPD patients","volume":"46","author":"Takahashi","year":"2015","journal-title":"Allergy Immunol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"3357","DOI":"10.18632\/oncotarget.6581","article-title":"Detection of EpCAM-positive microparticles in pleural fluid: A new approach to mini-invasively identify patients with malignant pleural effusions","volume":"7","author":"Roca","year":"2015","journal-title":"Oncotarget"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"1636","DOI":"10.1161\/STROKEAHA.106.479733","article-title":"Are Circulating Endothelial-Derived and Platelet-Derived Microparticles a Pathogenic Factor in the Cisplatin-Induced Stroke?","volume":"38","author":"Periard","year":"2007","journal-title":"Stroke"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1164\/rccm.201209-1697OC","article-title":"Endothelial Microparticles in Mild COPD and Emphysema: The MESA COPD Study","volume":"188","author":"Thomashow","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"224","DOI":"10.1164\/rccm.201012-2061OC","article-title":"Circulating Endothelial Microparticles as a Measure of Early Lung Destruction in Cigarette Smokers","volume":"184","author":"Gordon","year":"2011","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1111\/j.1538-7836.2005.01384.x","article-title":"Endothelial microparticles induce formation of platelet aggregates via a von Willebrand factor\/ristocetin dependent pathway, rendering them resistant to dissociation","volume":"3","author":"Jy","year":"2005","journal-title":"J. Thromb. Haemost."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"299","DOI":"10.1097\/SHK.0000000000000509","article-title":"Circulating Microparticles, Blood Cells, and Endothelium Induce Procoagulant Activity in Sepsis Through Phosphatidylserine Exposure","volume":"45","author":"Zhang","year":"2016","journal-title":"Shock"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"2432","DOI":"10.1182\/blood-2007-02-069997","article-title":"Activation of plasminogen into plasmin at the surface of endothelial microparticles: A mechanism that modulates angiogenic properties of endothelial progenitor cells in vitro","volume":"110","author":"Lacroix","year":"2007","journal-title":"Blood"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.3324\/haematol.2012.066167","article-title":"Leukocyte- and endothelial-derived microparticles: A circulating source for fibrinolysis","volume":"97","author":"Lacroix","year":"2012","journal-title":"Haematology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1097\/SHK.0000000000000331","article-title":"Enhanced Expression of Cell-Specific Surface Antigens on Endothelial Microparticles in Sepsis-Induced Disseminated Intravascular Coagulation","volume":"43","author":"Matsumoto","year":"2015","journal-title":"Shock"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"580","DOI":"10.1126\/science.1181928","article-title":"Platelets Amplify Inflammation in Arthritis via Collagen-Dependent Microparticle Production","volume":"327","author":"Boilard","year":"2010","journal-title":"Science"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.bbrc.2010.08.095","article-title":"Microparticles mediate enzyme transfer from platelets to mast cells: A new pathway for lipoxin A4 biosynthesis","volume":"400","author":"Tang","year":"2010","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"728","DOI":"10.1161\/ATVBAHA.109.200964","article-title":"Tissue Factor\u2013Bearing Microparticles and Thrombus Formation","volume":"31","author":"Zwicker","year":"2011","journal-title":"Arter. Thromb. Vasc. Biol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1318","DOI":"10.1189\/jlb.0405193","article-title":"Leukocyte-versus microparticle-mediated tissue factor transfer during arteriolar thrombus development","volume":"78","author":"Gross","year":"2005","journal-title":"J. Leukoc. Biol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"5503","DOI":"10.1182\/blood-2010-09-309161","article-title":"Complement activation on platelet-leukocyte complexes and microparticles in enterohemorrhagic Escherichia coli\u2013induced hemolytic uremic syndrome","volume":"117","author":"Sartz","year":"2011","journal-title":"Blood"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1182\/blood-2005-01-0220","article-title":"ICAM-1 on exosomes from mature dendritic cells is critical for efficient naive T-cell priming","volume":"106","author":"Segura","year":"2005","journal-title":"Blood"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1186\/s40560-018-0340-6","article-title":"Role of extracellular vesicles in the development of sepsis-induced coagulopathy","volume":"6","author":"Iba","year":"2018","journal-title":"J. Intensiv. Care"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"1603","DOI":"10.1016\/j.jaci.2014.11.026","article-title":"Exosome secretion by eosinophils: A possible role in asthma pathogenesis","volume":"135","author":"Mazzeo","year":"2015","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"2886","DOI":"10.4049\/jimmunol.1601810","article-title":"Neutrophil Microparticles Deliver Active Myeloperoxidase to Injured Mucosa To Inhibit Epithelial Wound Healing","volume":"198","author":"Slater","year":"2017","journal-title":"J. Immunol."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"510","DOI":"10.1182\/blood-2012-05-431114","article-title":"Antibacterial effect of microvesicles released from human neutrophilic granulocytes","volume":"121","author":"Timar","year":"2013","journal-title":"Blood"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"1260","DOI":"10.1111\/all.14103","article-title":"Immune complexes exposed on mast cell-derived nanovesicles amplify allergic inflammation","volume":"75","author":"Molfetta","year":"2019","journal-title":"Allergy"},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"788","DOI":"10.1016\/j.jaci.2017.07.040","article-title":"Mast cell exosomes can suppress allergic reactions by binding to IgE","volume":"141","author":"Xie","year":"2018","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"3382","DOI":"10.4049\/jimmunol.1600614","article-title":"Mast Cell Degranulation Is Accompanied by the Release of a Selective Subset of Extracellular Vesicles That Contain Mast Cell\u2013Specific Proteases","volume":"197","author":"Kormelink","year":"2016","journal-title":"J. Immunol."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1186\/s12931-020-01346-8","article-title":"Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells","volume":"21","author":"Yin","year":"2020","journal-title":"Respir. Res."},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"876","DOI":"10.1016\/j.ijcard.2016.11.302","article-title":"Monocyte-derived circulating microparticles (CD14+, CD14+\/CD11b+ and CD14+\/CD142+) are related to long-term prognosis for cardiovascular mortality in STEMI patients","volume":"227","author":"Bratseth","year":"2017","journal-title":"Int. J. Cardiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.3389\/fimmu.2018.01463","article-title":"LPS-Challenged Macrophages Release Microvesicles Coated with Histones","volume":"9","author":"Nair","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"435","DOI":"10.1038\/s41467-018-02865-1","article-title":"Activated CD8+ T cell extracellular vesicles prevent tumour progression by targeting of lesional mesenchymal cells","volume":"9","author":"Seo","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"1377","DOI":"10.1007\/s00296-019-04337-1","article-title":"Characterization of cell-derived microparticles in synovial fluid and plasma of patients with rheumatoid arthritis","volume":"39","author":"Michael","year":"2019","journal-title":"Rheumatol. Int."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"107","DOI":"10.2217\/bmm-2016-0261","article-title":"Identification of CD4+ T-cell-derived CD161+ CD39+ and CD39+CD73+ microparticles as new biomarkers for rheumatoid arthritis","volume":"11","author":"Fan","year":"2017","journal-title":"Biomark. Med."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"166","DOI":"10.1053\/j.gastro.2012.03.040","article-title":"Abnormal Plasma Microparticles Impair Vasoconstrictor Responses in Patients with Cirrhosis","volume":"143","author":"Rautou","year":"2012","journal-title":"Gastroenterology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"738","DOI":"10.1016\/j.immuni.2019.01.010","article-title":"Specific decrease in B-cell-derived extracellular vesicles enhances post-chemotherapeutic CD8+ T cell responses","volume":"50","author":"Zhang","year":"2019","journal-title":"Immunity"},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"1173","DOI":"10.1172\/JCI81131","article-title":"Regulation of chronic inflammatory and immune processes by extracellular vesicles","volume":"126","author":"Robbins","year":"2016","journal-title":"J. Clin. Investig."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"977","DOI":"10.1096\/fj.03-1094fje","article-title":"Adhesion and signaling by B cell-derived exosomes: The role of integrins","volume":"18","author":"Clayton","year":"2004","journal-title":"FASEB J."},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1111\/jth.13065","article-title":"Growing thrombi release increased levels of CD235a+ microparticles and decreased levels of activated platelet-derived microparticles. Validation in ST-elevation myocardial infarction patients","volume":"13","author":"Suades","year":"2015","journal-title":"J. Thromb. Haemost."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Koshiar, R.L., Somajo, S., Norstr\u00f6m, E., and Dahlb\u00e4ck, B. (2014). Erythrocyte-Derived Microparticles Supporting Activated Protein C-Mediated Regulation of Blood Coagulation. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0104200"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"1513","DOI":"10.3324\/haematol.2009.008938","article-title":"Circulating erythrocyte-derived microparticles are associated with coagulation activation in sickle cell disease","volume":"94","author":"Schaap","year":"2009","journal-title":"Haematology"},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1146\/annurev-cellbio-101512-122326","article-title":"Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles","volume":"30","author":"Colombo","year":"2014","journal-title":"Annu. Rev. Cell Dev. Biol."},{"key":"ref_75","first-page":"161","article-title":"Cancer: Diagnosis by extracellular vesicles","volume":"523","year":"2015","journal-title":"Nat. Cell Biol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"1286095","DOI":"10.1080\/20013078.2017.1286095","article-title":"Obstacles and opportunities in the functional analysis of extracellular vesicle RNA\u2014An ISEV position paper","volume":"6","author":"Mateescu","year":"2017","journal-title":"J. Extracell. Vesicles"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"453","DOI":"10.1007\/s00281-018-0682-0","article-title":"Molecular interactions at the surface of extracellular vesicles","volume":"40","year":"2018","journal-title":"Semin. Immunopathol."},{"key":"ref_78","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1039\/C7NR08360B","article-title":"Technical challenges of working with extracellular vesicles","volume":"10","author":"Ramirez","year":"2018","journal-title":"Nanoscale"},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"494","DOI":"10.1021\/acsnano.7b07060","article-title":"Multiplexed Profiling of Single Extracellular Vesicles","volume":"12","author":"Lee","year":"2018","journal-title":"ACS Nano"},{"key":"ref_80","doi-asserted-by":"crossref","first-page":"68","DOI":"10.3389\/fcvm.2017.00068","article-title":"A Protocol for Improved Precision and Increased Confidence in Nanoparticle Tracking Analysis Concentration Measurements between 50 and 120 nm in Biological Fluids","volume":"4","author":"Parsons","year":"2017","journal-title":"Front. Cardiovasc. Med."},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"801","DOI":"10.1038\/s41416-019-0726-9","article-title":"Tumour-derived extracellular vesicles in blood of metastatic cancer patients associate with overall survival","volume":"122","author":"Nanou","year":"2020","journal-title":"Br. J. Cancer"},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1632","DOI":"10.1161\/CIRCRESAHA.117.309417","article-title":"Methodological Guidelines to Study Extracellular Vesicles","volume":"120","author":"Coumans","year":"2017","journal-title":"Circ. Res."},{"key":"ref_83","doi-asserted-by":"crossref","unstructured":"Szatanek, R., Baj-Krzyworzeka, M., Zimoch, J., Lekka, M., Siedlar, M., and Baran, J. (2017). The Methods of Choice for Extracellular Vesicles (EVs) Characterization. Int. J. Mol. Sci., 18.","DOI":"10.3390\/ijms18061153"},{"key":"ref_84","doi-asserted-by":"crossref","unstructured":"Akers, J.C., Ramakrishnan, V., Nolan, J.P., Duggan, E., Fu, C.-C., Hochberg, F.H., Chen, C.C., and Carter, B.S. (2016). Comparative Analysis of Technologies for Quantifying Extracellular Vesicles (EVs) in Clinical Cerebrospinal Fluids (CSF). PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0149866"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1161\/CIRCRESAHA.117.311215","article-title":"Extracellular Vesicles and the Application of System Biology and Computational Modeling in Cardiac Repair","volume":"123","author":"Garikipati","year":"2018","journal-title":"Circ. Res."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"1713526","DOI":"10.1080\/20013078.2020.1713526","article-title":"MIFlowCyt-EV: A framework for standardized reporting of extracellular vesicle flow cytometry experiments","volume":"9","author":"Welsh","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"ref_87","doi-asserted-by":"crossref","first-page":"1326","DOI":"10.3389\/fimmu.2018.01326","article-title":"Systematic Methodological Evaluation of a Multiplex Bead-Based Flow Cytometry Assay for Detection of Extracellular Vesicle Surface Signatures","volume":"9","author":"Wiklander","year":"2018","journal-title":"Front. Immunol."},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1016\/j.transci.2015.10.008","article-title":"Tips and tricks for flow cytometry-based analysis and counting of microparticles","volume":"53","author":"Poncelet","year":"2015","journal-title":"Transfus. Apher. Sci."},{"key":"ref_89","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1016\/j.thromres.2009.12.019","article-title":"Flow cytometric detection of endothelial microparticles (EMP): Effects of centrifugation and storage alter with the phenotype studied","volume":"125","author":"Conraads","year":"2010","journal-title":"Thromb. Res."},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"190","DOI":"10.1111\/j.1538-7836.2008.03200.x","article-title":"Standardization of platelet-derived microparticle counting using calibrated beads and a Cytomics FC500 routine flow cytometer: A first step towards multicenter studies?","volume":"7","author":"Robert","year":"2009","journal-title":"J. Thromb. Haemost."},{"key":"ref_91","doi-asserted-by":"crossref","first-page":"428","DOI":"10.1016\/j.cell.2019.02.029","article-title":"Reassessment of Exosome Composition","volume":"177","author":"Jeppesen","year":"2019","journal-title":"Cell"},{"key":"ref_92","doi-asserted-by":"crossref","first-page":"772","DOI":"10.1016\/j.jacc.2006.10.053","article-title":"Cellular Origins and Thrombogenic Activity of Microparticles Isolated from Human Atherosclerotic Plaques","volume":"49","author":"Leroyer","year":"2007","journal-title":"J. Am. Coll. Cardiol."},{"key":"ref_93","doi-asserted-by":"crossref","first-page":"103","DOI":"10.1186\/s13075-015-0617-2","article-title":"Enhanced activation of dendritic cells by autologous apoptotic microvesicles in MRL\/lpr mice","volume":"17","author":"Dieker","year":"2015","journal-title":"Arthritis Res. Ther."},{"key":"ref_94","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ijcard.2016.01.211","article-title":"CD3(+)\/CD45(+) and SMA-\u03b1(+) circulating microparticles are increased in individuals at high cardiovascular risk who will develop a major cardiovascular event","volume":"208","author":"Suades","year":"2016","journal-title":"Int. J. Cardiol."},{"key":"ref_95","doi-asserted-by":"crossref","unstructured":"Fischer, S., Cornils, K., Speiseder, T., Badbaran, A., Reimer, R., Indenbirken, D., Grundhoff, A., Brunswig-Spickenheier, B., Alawi, M., and Lange, C. (2016). Indication of Horizontal DNA Gene Transfer by Extracellular Vesicles. PLoS ONE, 11.","DOI":"10.1371\/journal.pone.0163665"},{"key":"ref_96","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/cr.2014.44","article-title":"Double-stranded DNA in exosomes: A novel biomarker in cancer detection","volume":"24","author":"Thakur","year":"2014","journal-title":"Cell Res."},{"key":"ref_97","doi-asserted-by":"crossref","first-page":"824","DOI":"10.1111\/cas.13222","article-title":"Extracellular vesicles as trans-genomic agents: Emerging roles in disease and evolution","volume":"108","author":"Kawamura","year":"2017","journal-title":"Cancer Sci."},{"key":"ref_98","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.cell.2016.05.034","article-title":"Digestion of Chromatin in Apoptotic Cell Microparticles Prevents Autoimmunity","volume":"166","author":"Sisirak","year":"2016","journal-title":"Cell"},{"key":"ref_99","doi-asserted-by":"crossref","first-page":"1658","DOI":"10.1161\/CIRCRESAHA.117.309681","article-title":"Extracellular Vesicles in Angiogenesis","volume":"120","author":"Todorova","year":"2017","journal-title":"Circ. Res."},{"key":"ref_100","doi-asserted-by":"crossref","first-page":"1987","DOI":"10.3389\/fimmu.2019.01987","article-title":"Airway Epithelial Cells Generate Pro-Inflammatory Tenascin-C and Small Extracellular Vesicles in Response to TLR3 Stimuli and Rhinovirus Infection","volume":"10","author":"Mills","year":"2019","journal-title":"Front. Immunol."},{"key":"ref_101","doi-asserted-by":"crossref","first-page":"e2016","DOI":"10.1038\/cddis.2015.282","article-title":"Lung epithelial cell-derived extracellular vesicles activate macrophage-mediated inflammatory responses via ROCK1 pathway","volume":"6","author":"Moon","year":"2015","journal-title":"Cell Death Dis."},{"key":"ref_102","doi-asserted-by":"crossref","first-page":"1194","DOI":"10.1016\/j.jaci.2012.12.1565","article-title":"Proinflammatory role of epithelial cell\u2013derived exosomes in allergic airway inflammation","volume":"131","author":"Kulshreshtha","year":"2013","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_103","doi-asserted-by":"crossref","first-page":"4206","DOI":"10.4049\/jimmunol.1000409","article-title":"T Cell-Induced Mast Cell Activation: A Role for Microparticles Released from Activated T Cells","volume":"185","author":"Shefler","year":"2010","journal-title":"J. Immunol."},{"key":"ref_104","doi-asserted-by":"crossref","first-page":"921","DOI":"10.1189\/jlb.0807551","article-title":"Stimulated T cells generate microparticles, which mimic cellular contact activation of human monocytes: Differential regulation of pro- and anti-inflammatory cytokine production by high-density lipoproteins","volume":"83","author":"Scanu","year":"2008","journal-title":"J. Leukoc. Biol."},{"key":"ref_105","doi-asserted-by":"crossref","first-page":"220","DOI":"10.1016\/j.molimm.2013.01.017","article-title":"Lymphocyte-derived microparticles induce bronchial epithelial cells\u2019 pro-inflammatory cytokine production and apoptosis","volume":"55","author":"Qiu","year":"2013","journal-title":"Mol. Immunol."},{"key":"ref_106","doi-asserted-by":"crossref","first-page":"12641","DOI":"10.1038\/s41598-018-31013-4","article-title":"Circulating H3Cit is elevated in a human model of endotoxemia and can be detected bound to microvesicles","volume":"8","author":"Lasselin","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_107","doi-asserted-by":"crossref","first-page":"2512","DOI":"10.1182\/blood-2008-02-140533","article-title":"Annexin 1 mediates the rapid anti-inflammatory effects of neutrophil-derived microparticles","volume":"112","author":"Dalli","year":"2008","journal-title":"Blood"},{"key":"ref_108","doi-asserted-by":"crossref","unstructured":"Peruzzotti-Jametti, L., Bernstock, J.D., Willis, C.M., Manferrari, G., Rogall, R., Fernandez-Vizarra, E., Williamson, J.C., Braga, A., Bosch, A.V.D., and Leonardi, T. (2021). Neural stem cells traffic functional mitochondria via extracellular vesicles. PLoS Biol., 19.","DOI":"10.1371\/journal.pbio.3001166"},{"key":"ref_109","doi-asserted-by":"crossref","unstructured":"Nawaz, M., Shah, N., Zanetti, B.R., Maugeri, M., Silvestre, R.N., Fatima, F., Neder, L., and Valadi, H. (2018). Extracellular Vesicles and Matrix Remodeling Enzymes: The Emerging Roles in Extracellular Matrix Remodeling, Progression of Diseases and Tissue Repair. Cells, 7.","DOI":"10.3390\/cells7100167"},{"key":"ref_110","first-page":"608","article-title":"Extracellular Vesicles: Potential Roles in Regenerative Medicine","volume":"5","author":"Schiffelers","year":"2014","journal-title":"Front. Immunol."},{"key":"ref_111","first-page":"583","article-title":"Exosomes released during reticulocyte maturation bind to fibronectin via integrin \u03b14\u03b21","volume":"267","author":"Rieu","year":"2000","journal-title":"JBIC J. Biol. Inorg. Chem."},{"key":"ref_112","doi-asserted-by":"crossref","first-page":"385","DOI":"10.1681\/ASN.2012101031","article-title":"TGF-\u03b21\u2013Containing Exosomes from Injured Epithelial Cells Activate Fibroblasts to Initiate Tissue Regenerative Responses and Fibrosis","volume":"24","author":"Borges","year":"2012","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_113","doi-asserted-by":"crossref","first-page":"2892","DOI":"10.1073\/pnas.0409781102","article-title":"The induction of matrix metalloproteinase and cytokine expression in synovial fibroblasts stimulated with immune cell microparticles","volume":"102","author":"Distler","year":"2005","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_114","doi-asserted-by":"crossref","first-page":"2337","DOI":"10.1111\/febs.14485","article-title":"Monocyte-derived extracellular vesicles stimulate cytokine secretion and gene expression of matrix metalloproteinases by mesenchymal stem\/stromal cells","volume":"285","author":"Gebraad","year":"2018","journal-title":"FEBS J."},{"key":"ref_115","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/j.matbio.2017.10.007","article-title":"Proteases and glycosidases on the surface of exosomes: Newly discovered mechanisms for extracellular remodeling","volume":"75\u201376","author":"Sanderson","year":"2019","journal-title":"Matrix Biol."},{"key":"ref_116","doi-asserted-by":"crossref","first-page":"13202","DOI":"10.1074\/jbc.M115.638759","article-title":"Rapid Trimming of Cell Surface Polysialic Acid (PolySia) by Exovesicular Sialidase Triggers Release of Preexisting Surface Neurotrophin","volume":"290","author":"Sumida","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_117","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/j.cell.2020.05.050","article-title":"Microglial Remodeling of the Extracellular Matrix Promotes Synapse Plasticity","volume":"182","author":"Nguyen","year":"2020","journal-title":"Cell"},{"key":"ref_118","doi-asserted-by":"crossref","first-page":"979","DOI":"10.1189\/jlb.4MR0316-102R","article-title":"Angiogenesis and wound repair: When enough is enough","volume":"100","author":"DiPietro","year":"2016","journal-title":"J. Leukoc. Biol."},{"key":"ref_119","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1016\/j.canlet.2017.06.012","article-title":"The roles of integrin \u03b1v\u03b26 in cancer","volume":"403","author":"Niu","year":"2017","journal-title":"Cancer Lett."},{"key":"ref_120","doi-asserted-by":"crossref","first-page":"1763594","DOI":"10.1080\/20013078.2020.1763594","article-title":"The \u03b1v\u03b26 integrin in cancer cell-derived small extracellular vesicles enhances angiogenesis","volume":"9","author":"Krishn","year":"2020","journal-title":"J. Extracell. Vesicles"},{"key":"ref_121","doi-asserted-by":"crossref","first-page":"1429","DOI":"10.1172\/JCI36183","article-title":"Biomarkers for epithelial-mesenchymal transitions","volume":"119","author":"Zeisberg","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_122","doi-asserted-by":"crossref","first-page":"760","DOI":"10.1136\/thoraxjnl-2013-204608","article-title":"Epithelial\u2013mesenchymal transition in lung development and disease: Does it exist and is it important?","volume":"69","author":"Bartis","year":"2014","journal-title":"Thorax"},{"key":"ref_123","doi-asserted-by":"crossref","first-page":"1420","DOI":"10.1172\/JCI39104","article-title":"The basics of epithelial-mesenchymal transition","volume":"119","author":"Kalluri","year":"2009","journal-title":"J. Clin. Investig."},{"key":"ref_124","doi-asserted-by":"crossref","unstructured":"Kim, H., Lee, S., Shin, E., Seong, K.M., Jin, Y.W., Youn, H., and Youn, B. (2020). The Emerging Roles of Exosomes as EMT Regulators in Cancer. Cells, 9.","DOI":"10.3390\/cells9040861"},{"key":"ref_125","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1172\/JCI122478","article-title":"APC-activated long noncoding RNA inhibits colorectal carcinoma pathogenesis through reduction of exosome production","volume":"129","author":"Wang","year":"2019","journal-title":"J. Clin. Investig."},{"key":"ref_126","doi-asserted-by":"crossref","first-page":"581","DOI":"10.1093\/qjmed\/hcz093","article-title":"Snail1-dependent cancer-associated fibroblasts induce epithelial-mesenchymal transition in lung cancer cells via exosomes","volume":"112","author":"You","year":"2019","journal-title":"QJM Int. J. Med."},{"key":"ref_127","doi-asserted-by":"crossref","first-page":"3197","DOI":"10.2147\/OTT.S192218","article-title":"Exosomal Tenascin-c induces proliferation and invasion of pancreatic cancer cells by WNT signaling","volume":"12","author":"Qian","year":"2019","journal-title":"OncoTargets Ther."},{"key":"ref_128","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1681\/ASN.2018121251","article-title":"Cellular Senescence in the Kidney","volume":"30","author":"Docherty","year":"2019","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_129","doi-asserted-by":"crossref","first-page":"14532","DOI":"10.1038\/ncomms14532","article-title":"Cellular senescence mediates fibrotic pulmonary disease","volume":"8","author":"Schafer","year":"2017","journal-title":"Nat. Commun."},{"key":"ref_130","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1016\/j.cmet.2020.06.004","article-title":"Small Extracellular Vesicles Have GST Activity and Ameliorate Senescence-Related Tissue Damage","volume":"32","author":"Labora","year":"2020","journal-title":"Cell Metab."},{"key":"ref_131","doi-asserted-by":"crossref","first-page":"329","DOI":"10.1016\/j.cmet.2019.05.015","article-title":"Extracellular Vesicle-Contained eNAMPT Delays Aging and Extends Lifespan in Mice","volume":"30","author":"Yoshida","year":"2019","journal-title":"Cell Metab."},{"key":"ref_132","doi-asserted-by":"crossref","first-page":"3956","DOI":"10.1016\/j.celrep.2019.05.095","article-title":"Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3","volume":"27","author":"Borghesan","year":"2019","journal-title":"Cell Rep."},{"key":"ref_133","doi-asserted-by":"crossref","first-page":"1527","DOI":"10.1164\/rccm.201708-1580OC","article-title":"Increased Extracellular Vesicles Mediate WNT5A Signaling in Idiopathic Pulmonary Fibrosis","volume":"198","author":"Lehmann","year":"2018","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_134","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1182\/blood-2018-07-818211","article-title":"The multifaceted role of fibrinogen in tissue injury and inflammation","volume":"133","author":"Luyendyk","year":"2019","journal-title":"Blood"},{"key":"ref_135","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1002\/JLB.3MR0120-390R","article-title":"Eosinophils in wound healing and epithelial remodeling: Is coagulation a missing link?","volume":"108","author":"Coden","year":"2020","journal-title":"J. Leukoc. Biol."},{"key":"ref_136","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.2337\/diabetes.54.7.2143","article-title":"Tissue Factor as a Link Between Wounding and Tissue Repair","volume":"54","author":"Chen","year":"2005","journal-title":"Diabetes"},{"key":"ref_137","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1111\/j.1365-2133.2004.06261.x","article-title":"Epithelial tissue-type plasminogen activator expression, unlike that of urokinase, its receptor, and plasminogen activator inhibitor-1, is increased in chronic venous ulcers","volume":"151","author":"Weckroth","year":"2004","journal-title":"Br. J. Dermatol."},{"key":"ref_138","doi-asserted-by":"crossref","first-page":"L1035","DOI":"10.1152\/ajplung.00214.2009","article-title":"Procoagulant alveolar microparticles in the lungs of patients with acute respiratory distress syndrome","volume":"297","author":"Bastarache","year":"2009","journal-title":"Am. J. Physiol. Cell. Mol. Physiol."},{"key":"ref_139","doi-asserted-by":"crossref","first-page":"608","DOI":"10.1136\/thx.2006.063305","article-title":"The alveolar epithelium can initiate the extrinsic coagulation cascade through expression of tissue factor","volume":"62","author":"Bastarache","year":"2007","journal-title":"Thorax"},{"key":"ref_140","doi-asserted-by":"crossref","first-page":"412","DOI":"10.1136\/thoraxjnl-2020-216243","article-title":"COVID-19, immunothrombosis and venous thromboembolism: Biological mechanisms","volume":"76","author":"Loo","year":"2021","journal-title":"Thorax"},{"key":"ref_141","doi-asserted-by":"crossref","first-page":"878","DOI":"10.1161\/ATVBAHA.120.315547","article-title":"Patients With COVID-19 Have Elevated Levels of Circulating Extracellular Vesicle Tissue Factor Activity That Is Associated With Severity and Mortality\u2014Brief Report","volume":"41","author":"Rosell","year":"2021","journal-title":"Arterioscler. Thromb. Vasc. Biol."},{"key":"ref_142","doi-asserted-by":"crossref","first-page":"1284","DOI":"10.1161\/CIRCRESAHA.110.233056","article-title":"Microparticles in Hemostasis and Thrombosis","volume":"108","author":"Owens","year":"2011","journal-title":"Circ. Res."},{"key":"ref_143","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1055\/s-0036-1592301","article-title":"Microparticles and Fibrinolysis","volume":"43","author":"Vallier","year":"2016","journal-title":"Semin. Thromb. Hemost."},{"key":"ref_144","doi-asserted-by":"crossref","first-page":"2017","DOI":"10.1681\/ASN.2013101066","article-title":"Human Urinary Exosomes as Innate Immune Effectors","volume":"25","author":"Hiemstra","year":"2014","journal-title":"J. Am. Soc. Nephrol."},{"key":"ref_145","doi-asserted-by":"crossref","first-page":"1858","DOI":"10.1096\/fj.08-119131","article-title":"Characterization of exosome-like vesicles released from human tracheobronchial ciliated epithelium: A possible role in innate defense","volume":"23","author":"Kesimer","year":"2009","journal-title":"FASEB J."},{"key":"ref_146","doi-asserted-by":"crossref","first-page":"1393","DOI":"10.1172\/JCI72094","article-title":"Bitter and sweet taste receptors regulate human upper respiratory innate immunity","volume":"124","author":"Lee","year":"2014","journal-title":"J. Clin. Investig."},{"key":"ref_147","doi-asserted-by":"crossref","first-page":"1131","DOI":"10.1126\/science.1173869","article-title":"Motile Cilia of Human Airway Epithelia Are Chemosensory","volume":"325","author":"Shah","year":"2009","journal-title":"Science"},{"key":"ref_148","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/bs.mcb.2017.07.004","article-title":"Characterization of GPCRs in extracellular vesicle (EV)","volume":"142","author":"Medapati","year":"2017","journal-title":"Methods Cell Biol."},{"key":"ref_149","doi-asserted-by":"crossref","first-page":"627","DOI":"10.1016\/j.tips.2020.07.001","article-title":"The Convergence of Extracellular Vesicle and GPCR Biology","volume":"41","author":"Bebelman","year":"2020","journal-title":"Trends Pharmacol. Sci."},{"key":"ref_150","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.jaci.2014.05.049","article-title":"Epithelial barrier function: At the front line of asthma immunology and allergic airway inflammation","volume":"134","author":"Georas","year":"2014","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_151","doi-asserted-by":"crossref","first-page":"1499","DOI":"10.1016\/j.jaci.2020.04.010","article-title":"Epithelial barriers in allergy and asthma","volume":"145","author":"Hellings","year":"2020","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_152","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1164\/rccm.201704-0667OC","article-title":"Exhaustion of Airway Basal Progenitor Cells in Early and Established Chronic Obstructive Pulmonary Disease","volume":"197","author":"Ghosh","year":"2018","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_153","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1136\/thx.52.3.213","article-title":"Epithelial barrier formation by airway basal cells","volume":"52","author":"Erjefalt","year":"1997","journal-title":"Thorax"},{"key":"ref_154","doi-asserted-by":"crossref","first-page":"752","DOI":"10.1016\/j.devcel.2018.03.001","article-title":"Spatial-Temporal Lineage Restrictions of Embryonic p63+ Progenitors Establish Distinct Stem Cell Pools in Adult Airways","volume":"44","author":"Yang","year":"2018","journal-title":"Dev. Cell"},{"key":"ref_155","doi-asserted-by":"crossref","unstructured":"Meng, J., Zhou, P., Liu, Y., Liu, F., Yi, X., Liu, S., Holtappels, G., Bachert, C., and Zhang, N. (2013). The Development of Nasal Polyp Disease Involves Early Nasal Mucosal Inflammation and Remodelling. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0082373"},{"key":"ref_156","doi-asserted-by":"crossref","first-page":"49","DOI":"10.1164\/rccm.201207-1292OC","article-title":"Excessive Fibrin Deposition in Nasal Polyps Caused by Fibrinolytic Impairment through Reduction of Tissue Plasminogen Activator Expression","volume":"187","author":"Takabayashi","year":"2013","journal-title":"Am. J. Respir. Crit. Care Med."},{"key":"ref_157","doi-asserted-by":"crossref","first-page":"1387","DOI":"10.1016\/j.jaci.2012.03.005","article-title":"Eosinophilic esophagitis: Epithelial mesenchymal transition contributes to esophageal remodeling and reverses with treatment","volume":"129","author":"Kagalwalla","year":"2012","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_158","doi-asserted-by":"crossref","first-page":"1512","DOI":"10.1016\/j.jaip.2017.04.024","article-title":"Biotherapeutics in Chronic Rhinosinusitis with and without Nasal Polyps","volume":"5","author":"Bachert","year":"2017","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_159","doi-asserted-by":"crossref","first-page":"1989","DOI":"10.1016\/j.bbamem.2013.04.018","article-title":"EpCAM: Structure and function in health and disease","volume":"1828","author":"Schnell","year":"2013","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_160","doi-asserted-by":"crossref","first-page":"2241","DOI":"10.1242\/jcs.108.6.2241","article-title":"Expression of the beta 6 integrin subunit in development, neoplasia and tissue repair suggests a role in epithelial remodeling","volume":"108","author":"Breuss","year":"1995","journal-title":"J. Cell Sci."},{"key":"ref_161","doi-asserted-by":"crossref","first-page":"42","DOI":"10.1111\/1523-1747.ep12327199","article-title":"Keratinocytes in human wounds express alpha v beta 6 integrin","volume":"106","author":"Haapasalmi","year":"1996","journal-title":"J. Investig. Dermatol."},{"key":"ref_162","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1016\/S0002-9440(10)63113-6","article-title":"Increased expression of beta6-integrin in skin leads to spontaneous development of chronic wounds","volume":"164","author":"Hakkinen","year":"2004","journal-title":"Am. J. Pathol."},{"key":"ref_163","doi-asserted-by":"crossref","first-page":"24677","DOI":"10.18632\/oncotarget.8250","article-title":"Epithelial-to-mesenchymal transition leads to loss of EpCAM and different physical properties in circulating tumor cells from metastatic breast cancer","volume":"7","author":"Hyun","year":"2016","journal-title":"Oncotarget"},{"key":"ref_164","doi-asserted-by":"crossref","first-page":"183503","DOI":"10.1016\/j.bbamem.2020.183503","article-title":"Role of tight junctions in the epithelial-to-mesenchymal transition of cancer cells","volume":"1863","author":"Kyuno","year":"2021","journal-title":"Biochim. Biophys. Acta Biomembr."},{"key":"ref_165","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1016\/j.jaci.2016.03.050","article-title":"Regulation of bronchial epithelial barrier integrity by type 2 cytokines and histone deacetylases in asthmatic patients","volume":"139","author":"Wawrzyniak","year":"2017","journal-title":"J. Allergy Clin. Immunol."},{"key":"ref_166","doi-asserted-by":"crossref","first-page":"415","DOI":"10.1038\/nrc2131","article-title":"Snail, Zeb and bHLH factors in tumour progression: An alliance against the epithelial phenotype?","volume":"7","author":"Peinado","year":"2007","journal-title":"Nat. Rev. Cancer"},{"key":"ref_167","doi-asserted-by":"crossref","first-page":"1053","DOI":"10.1136\/thx.2009.121798","article-title":"SNAI transcription factors mediate epithelial-mesenchymal transition in lung fibrosis","volume":"64","author":"Jayachandran","year":"2009","journal-title":"Thorax"},{"key":"ref_168","doi-asserted-by":"crossref","first-page":"663","DOI":"10.1016\/j.molonc.2015.12.006","article-title":"The transcription factors Slug (SNAI2) and Snail (SNAI1) regulate phospholipase D (PLD) promoter in opposite ways towards cancer cell invasion","volume":"10","author":"Ganesan","year":"2016","journal-title":"Mol. Oncol."},{"key":"ref_169","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1038\/nrm3758","article-title":"Molecular mechanisms of epithelial\u2013mesenchymal transition","volume":"15","author":"Lamouille","year":"2014","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_170","doi-asserted-by":"crossref","first-page":"1061","DOI":"10.1016\/j.jaip.2016.12.027","article-title":"Clinical Characteristics of Patients with Chronic Rhinosinusitis with Nasal Polyps, Asthma, and Aspirin-Exacerbated Respiratory Disease","volume":"5","author":"Stevens","year":"2017","journal-title":"J. Allergy Clin. Immunol. Pract."},{"key":"ref_171","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1002\/alr.21674","article-title":"Prevalence of confirmed asthma varies in chronic rhinosinusitis subtypes","volume":"6","author":"Promsopa","year":"2016","journal-title":"Int. Forum Allergy Rhinol."},{"key":"ref_172","doi-asserted-by":"crossref","first-page":"534","DOI":"10.1016\/j.chest.2018.10.007","article-title":"Tissue Factor Facilitates Wound Healing in Human Airway Epithelial Cells","volume":"155","author":"Davis","year":"2018","journal-title":"Chest"},{"key":"ref_173","doi-asserted-by":"crossref","first-page":"1234850","DOI":"10.1126\/science.1234850","article-title":"Epithelial Plasticity: A Common Theme in Embryonic and Cancer Cells","volume":"342","author":"Nieto","year":"2013","journal-title":"Science"},{"key":"ref_174","doi-asserted-by":"crossref","first-page":"1988","DOI":"10.1111\/all.13222","article-title":"Beyond epithelial-to-mesenchymal transition: Common suppression of differentiation programs underlies epithelial barrier dysfunction in mild, moderate, and severe asthma","volume":"72","author":"Loffredo","year":"2017","journal-title":"Allergy"},{"key":"ref_175","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.jaci.2018.10.056","article-title":"Chronic rhinosinusitis in elderly patients is associated with an exaggerated neutrophilic proinflammatory response to pathogenic bacteria","volume":"143","author":"Morse","year":"2019","journal-title":"J. Allergy Clin. Immunol."}],"container-title":["International Journal of Molecular Sciences"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/21\/11709\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T07:22:20Z","timestamp":1760167340000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/1422-0067\/22\/21\/11709"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,28]]},"references-count":175,"journal-issue":{"issue":"21","published-online":{"date-parts":[[2021,11]]}},"alternative-id":["ijms222111709"],"URL":"https:\/\/doi.org\/10.3390\/ijms222111709","relation":{},"ISSN":["1422-0067"],"issn-type":[{"value":"1422-0067","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,28]]}}}