{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:14:46Z","timestamp":1791360886398,"version":"4.3.1"},"reference-count":117,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,10,30]],"date-time":"2021-10-30T00:00:00Z","timestamp":1635552000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100007801","name":"Fundaci\u00f3n Seneca","doi-asserted-by":"publisher","award":["19881\/GERM\/15"],"award-info":[{"award-number":["19881\/GERM\/15"]}],"id":[{"id":"10.13039\/100007801","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003329","name":"Ministry of Economy, Industry and Competitiveness","doi-asserted-by":"publisher","award":["RED2018-102499-T"],"award-info":[{"award-number":["RED2018-102499-T"]}],"id":[{"id":"10.13039\/501100003329","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Spanish Ministry of Science and Innovation","doi-asserted-by":"publisher","award":["PID2019-106498GB-I00"],"award-info":[{"award-number":["PID2019-106498GB-I00"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>To analyze the neuroprotective effects of 7,8-Dihydroxyflavone (DHF) in vivo and ex vivo, adult albino Sprague-Dawley rats were given a left intraorbital optic nerve transection (IONT) and were divided in two groups: One was treated daily with intraperitoneal (ip) DHF (5 mg\/kg) (n = 24) and the other (n = 18) received ip vehicle (1% DMSO in 0.9% NaCl) from one day before IONT until processing. At 5, 7, 10, 12, 14, and 21 days (d) after IONT, full field electroretinograms (ERG) were recorded from both experimental and one additional na\u00efve-control group (n = 6). Treated rats were analyzed 7 (n = 14), 14 (n = 14) or 21 d (n = 14) after IONT, and the retinas immune stained against Brn3a, Osteopontin (OPN) and the T-box transcription factor T-brain 2 (Tbr2) to identify surviving retinal ganglion cells (RGCs) (Brn3a+), \u03b1-like (OPN+), \u03b1-OFF like (OPN+Brn3a+) or M4-like\/\u03b1-ON sustained RGCs (OPN+Tbr+). Na\u00efve and right treated retinas showed normal ERG recordings. Left vehicle-treated retinas showed decreased amplitudes of the scotopic threshold response (pSTR) (as early as 5 d), the rod b-wave, the mixed response and the cone response (as early as 10 d), which did not recover with time. In these retinas, by day 7 the total numbers of Brn3a+RGCs, OPN+RGCs and OPN+Tbr2+RGCs decreased to less than one half and OPN+Brn3a+RGCs decreased to approximately 0.5%, and Brn3a+RGCs showed a progressive loss with time, while OPN+RGCs and OPN+Tbr2+RGCs did not diminish after seven days. Compared to vehicle-treated, the left DHF-treated retinas showed significantly greater amplitudes of the pSTR, normal b-wave values and significantly greater numbers of OPN+RGCs and OPN+Tbr2+RGCs for up to 14 d and of Brn3a+RGCs for up to 21 days. DHF affords significant rescue of Brn3a+RGCs, OPN+RGCs and OPN+Tbr2+RGCs, but not OPN+Brn3a+RGCs, and preserves functional ERG responses after IONT.<\/jats:p>","DOI":"10.3390\/ijms222111815","type":"journal-article","created":{"date-parts":[[2021,11,1]],"date-time":"2021-11-01T22:21:34Z","timestamp":1635805294000},"page":"11815","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["7,8-Dihydroxiflavone Maintains Retinal Functionality and Protects Various Types of RGCs in Adult Rats with Optic Nerve Transection"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6770-7463","authenticated-orcid":false,"given":"Alejandro","family":"Gallego-Ortega","sequence":"first","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9352-1400","authenticated-orcid":false,"given":"Beatriz","family":"Vidal-Villegas","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"},{"name":"Servicio de Oftalmolog\u00eda, Hospital Cl\u00ednico San Carlos, Instituto de Investigaci\u00f3n Sanitaria del Hospital Cl\u00ednico San Carlos (IdIC), 28040 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1236-7702","authenticated-orcid":false,"given":"Mar\u00eda","family":"Norte-Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manuel","family":"Salinas-Navarro","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9711-2196","authenticated-orcid":false,"given":"Marcelino","family":"Avil\u00e9s-Trigueros","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5734-482X","authenticated-orcid":false,"given":"Mar\u00eda","family":"Villegas-P\u00e9rez","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9216-1615","authenticated-orcid":false,"given":"Manuel","family":"Vidal-Sanz","sequence":"additional","affiliation":[{"name":"Departamento de Oftalmolog\u00eda, Instituto Murciano de Investigaci\u00f3n Biosanitaria (IMIB) Virgen de la Arrixaca, Universidad de Murcia, Campus de CC de la Salud, El Palmar, 30120 Murcia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,10,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1749-6632.1987.tb23661.x","article-title":"Growth and connectivity of axotomized retinal neurons in adult rats with optic nerves substituted by PNS grafts linking the eye and the midbrain","volume":"495","author":"Aguayo","year":"1987","journal-title":"Ann. 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