{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T17:49:14Z","timestamp":1772819354994,"version":"3.50.1"},"reference-count":66,"publisher":"MDPI AG","issue":"21","license":[{"start":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T00:00:00Z","timestamp":1636243200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004955","name":"Austrian Research Promotion Agency","doi-asserted-by":"publisher","award":["866384"],"award-info":[{"award-number":["866384"]}],"id":[{"id":"10.13039\/501100004955","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJMS"],"abstract":"<jats:p>Deoxynivalenol (DON), a frequent mycotoxin worldwide, impairs human and animal health. The response of microRNAs, small non-coding RNAs, to DON has been scarcely investigated, but holds remarkable potential for biomarker applications. Hence, we aimed to investigate DON-induced changes in the microRNA expression in porcine liver, jejunum and serum by combining targeted and untargeted analyses. Piglets received uncontaminated feed or feed containing 900 \u00b5g\/kg and 2500 \u00b5g\/kg DON for four weeks, followed by a wash-out period. In tissue, only slight changes in microRNA expression were detected, with ssc-miR-10b being downregulated in liver of DON-exposed piglets. In serum, several microRNAs were differentially expressed upon DON exposure, four of which were validated by qPCR (ssc-miR-16, ssc-miR-128, ssc-miR-451, ssc-miR-205). The serum microRNA response to DON increased over time and declined after removal of contaminated diets. Receiver operating curve analyses for individual microRNAs were significant, and a combination of the four microRNAs increased the predictive capacity for DON exposure. Predicted microRNA target genes showed enrichment of several pathways including PIK3-AKT, Wnt\/\u03b2-catenin, and adherens junctions. This study gives, for the first time, a comprehensive view of the porcine microRNA response to DON, providing a basis for future research on microRNAs as biomarkers for mycotoxins.<\/jats:p>","DOI":"10.3390\/ijms222112043","type":"journal-article","created":{"date-parts":[[2021,11,7]],"date-time":"2021-11-07T20:41:14Z","timestamp":1636317674000},"page":"12043","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["MicroRNA Expression Profiling in Porcine Liver, Jejunum and Serum upon Dietary DON Exposure Reveals Candidate Toxicity Biomarkers"],"prefix":"10.3390","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2424-618X","authenticated-orcid":false,"given":"Maia","family":"Segura-Wang","sequence":"first","affiliation":[{"name":"BIOMIN Research Center, BIOMIN Holding GmbH, Technopark 1, 3430 Tulln, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Grenier","sequence":"additional","affiliation":[{"name":"BIOMIN Research Center, BIOMIN Holding GmbH, Technopark 1, 3430 Tulln, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzana","family":"Ilic","sequence":"additional","affiliation":[{"name":"BIOMIN Research Center, BIOMIN Holding GmbH, Technopark 1, 3430 Tulln, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6190-5984","authenticated-orcid":false,"given":"Ursula","family":"Ruczizka","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7016-5853","authenticated-orcid":false,"given":"Maximiliane","family":"Dippel","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7941-8850","authenticated-orcid":false,"given":"Moritz","family":"B\u00fcnger","sequence":"additional","affiliation":[{"name":"University Clinic for Swine, Department for Farm Animals and Veterinary Public Health, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Hackl","sequence":"additional","affiliation":[{"name":"TAmiRNA GmbH, Leberstrasse 20, 1110 Vienna, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8534-4149","authenticated-orcid":false,"given":"Veronika","family":"Nagl","sequence":"additional","affiliation":[{"name":"BIOMIN Research Center, BIOMIN Holding GmbH, Technopark 1, 3430 Tulln, Austria"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,11,7]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1016\/j.anifeedsci.2007.06.006","article-title":"Deoxynivalenol: Toxicity, Mechanisms and Animal Health Risks","volume":"137","author":"Pestka","year":"2007","journal-title":"Anim. 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